Laila M. Fadda1, Nouf M. Al-Rasheed1, Iman H. Hasan1, Hanaa M. Ali2,3*, Nawal M. Al-Rasheed1,4, Musaed Al-Fayez5, Aly M. Ahmed5, Nada Almutlaq1, Nehal Qasem1 and Reem Khalaf1
... hepatotoxicity. N-acetylcysteine (NAC) is the best antidote for APAP but may induce a variety of side effects. This study was designed to compare the potential impact of NAC with that of Thymoquinone (THQ), and/or Curcumin (CUR) either alone or in combination on liver injury induced by inflammation, oxidative stress in response to APAP toxicity in rats. Serum aminotransferases (ALT and AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), total prote...
Zaheer Abbas*, Shaukat Ali, Jalal-Ud-Din and Ghulam M. Ali*
...on period, addition of L-cysteine in co-cultivation medium, heat shock to immature embryos prior to infection, resting stage and Agrobacterium strains that deliver T-DNA into plant cell from which whole plant can be regenerated. The results indicated that optimal embryo physiological age is 15 and 18 days after pollination and transient transformation efficiency decreased at 22 days after pollination. Enhanced transient beta glucuronidase (GUS) expression with...
Amna Waseem, Sikander Ali* and Syeda Wajiha Khalid
... it with low levels of L-cysteine HCl to protect it from instability. It was observed that under optimum conditions at 30°C temperature, pH 7, and incubation period 64 h, the lipase activity was increased up to 124±2.48 U/g. The crude enzyme was purified by ammonium sulfate precipitation method at 40 %. The molecular weight of the enzyme was found to be 33 kDa. The results showed that the enzyme produced from mutant strain gave significantly higher ...
S. Justin Carlus1,*, Atiyeh M Abdallah1,2, Abdulhadi H. Al-Mazroea1, Mazen Khalid Al-Harbi3 and Khalid M Al-Harbi1
...d, and maternal hyperhomocysteinemia is an independent risk factor for CHD. In Saudi Arabia CHD represents one of the most important health problems. Here we examined the association between methylenetetrahydrofolate reductase (MTHFR) gene polymorphisms and CHD and DS co-inheritance in patients from Al Madinah, Saudi Arabia. MTHFR rs1801133 and rs1801131 polymorphisms were genotyped in 99 CHD patients with or without DS and 126 ethnically matched...
Zakirullah1, Hamayun Khan1, Ali Gohar2, Haq Amanullah1,*, Muhammad Younas1, Muhammad Tarique Tunio3, Muhammad Saleem Khan4, Ibrar Muhammad Khan1 and Noushad Khan5
...vorable application of L-cysteine for cryopreservation of Achai and Holstein Friesian bull semen under the subtropical conditions of Peshawar region of Pakistan. Semen of six adult bulls, three Achai (the indigenous breed) and three Holstein Friesian (exotic breed) amount to the experimental material at Government Cattle Breeding and Dairy Farm Harichand, Charsadda. Separate artificial vagina was utilized for semen collection from either breed, maintained at 4...
Sajida Sabahat1, Asif Nadeem1,*, Maryam Javed1, Muhammad Yasir Zahoor1, Abu Saeed Hashmi1, Ghulam Yasein2 and Ghulam Abbas1
Hala Rajab1, Muhammad Sayyar Khan1*, Safdar Hussain Shah1 and Syed Mehar Ali Shah2
...erproduce high levels of cysteine and glutathione for enhanced stress tolerance especially against heavy metals. ...
Tong Feng, Zilu Zhang, Minghao Qu, Chan Luo, Laiba Shafique, Qingyou Liu and Kuiqing Cui*
...tated from tryptophan to cysteine. The results of this study have important reference significance for the future correlation analysis between MC1R gene and Nubian goat wool color traits and the color genetic mechanism of Nubian goats.
...
Ahmet Ozer Sehirli1,2, Serkan Sayiner3*, Ayliz Velioglu-Ogunc4, Nedime Serakinci5, Emel Eksioglu-Demiralp6, Berrak Yegen7, Feriha Ercan8 andGoksel Sener2
... the presence of N-acetylcysteine (NAC). The renal mass of Wistar albino rats was reduced at a rate of 5/6. Captopril, Valsartan and NAC were applied intra-peritoneal alone or in combination. Blood pressure and heart rate were monitored at weekly intervals over a period of six weeks. Serum creatinine, blood urea nitrogen (BUN), lactate dehydrogenase (LDH) activity, cytokines (TNF-α, IL-1β, IL-6) concentrations, urinary volume, creatinine, and both s...
Masroor Ellahi Babar*
...morphic regions includes cysteine nucleotide deletion, AT bases insertion and transvertions (C>G; G>T). Deletion of A bases at c.1-1075 and hetrozygosity (CT) at the c1-1046 were analyzed along with some other SNPs are very important. These evidences points towards the change of expression of those protein which are nearest to the region of gene regulation and enhancer region. After observing taxas several distinct clads of camel breeds it was found that...
Ibrar Muhammad Khan1, Dezhi Xu1, Zubing Cao1, Hongyu Liu1, Adnan Khan2, Sajid Ur Rahman3, Jam Zaheer Ahmed4, Muhammad Akmal Raheem5 and Yunhai Zhang1*
...he antioxidants agents(L-cysteine and vitamin E) were added into semen diluent and assessed the freeze-thawed semen quality parameters. The ejaculates were collected from two crossbred groups (Sahiwal × Holstein-Friesian), and (Achai × Jersey) and, split into seven aliquots while extended with three different concentration (5, 7.5 and10 mmol) of L-cysteine diluents and followed by other three concen...
Alamaary Mohaammed Saad1,3, Abd Wahid Haron1*, Mohamed Ali2,
Mark Wen Han Hiew1 and Lawan Adamu1
...each, ten ejaculates for cysteine in experiment 1 and the other ten for ascorbic acid groups in experiment 2. Each ejaculate from an individual horse was divided into six equal parts of the study, and they were cryopreserved using the same procedure. Different concentrations of either cysteine (0, 0.25, 0.5, 1, 2, and 4mg/ml) or ascorbic acid (0, 0.25, 0.5, 1, 2, and 4mg/ml) were added to the semen of the first and second ex...
Junli Liu1, Tongfang Yang2 and Lisheng Wu3*
..., 2, 4 μg/ml. P21 and cysteine-containing aspartic protease 3 (Caspase-3) protein expression in U2OS cells were detected by western blot, thiazole blue (MTT) and plate cloning experiments to determine cell survival and colony formation, flow cytometry to measure apoptosis, and quantitative real-time polymerase chain reaction (qRT-PCR) to assay LINC00857 and miR-340 expression. Bioinformatics and dual luciferase reports analyzed the targeting relationship be...
Muhammad Hammad Fayyaz*, S. Murtaza Hassan Andrabi and Muhammad Shafiq Haider
... Glutathione (GLU) and L-cysteine (CYS) in the extender. In the first experiment, semen samples from each of the five mature bucks were pooled and diluted in Tris-citric acid (TCA) extender containing Glutathione (0.5mM, 1mM), L-cysteine (5mM CYS, 10mM CYS), and control. In experiment 2, semen samples (n=5) were diluted in TCA extender containing differential doses of combined antioxidants (0.5 mM GLU+ 5mM CYS, 0.5mM + 10mM ...
Hamdy Abdala Elnagar1, Wael Mohamed Wafa1*, Abdel-Khalek El-Sayed Abdel-Khalek2
...ows, Cytokines, N-acetyl-cysteine, Reproduction
...
Zheng-Yong Wen1, 2*, Chuan-Jie Qin1,2, Bin Li1,2, Rui Li1,2 and Xiao-Tao Shi3*
... a-helix domains and two cysteine residues were conserved in vertebrates. Three-dimensional (3D) structure modeling revealed that pvLeptin was highly conserved with that of other tetrapods. Genetic synteny analysis revealed that lepB had specifically lost in siluriformes teleosts. Phylogenetic analysis showed that fish lineage contained two clades of leptinA and leptinB, and the pvleptin was grouped into leptinA clade and shared a close relationship with its c...
Chalothon Amporn1, Somchit Guntaprom1*, Sarawut Duongmawong1, Wilasinee Srisanyong1, Sirikanda Thanasuwan1, Phalita Koonnadilokpot1, Dechawut Bunyaluk2, Juggrid Jugsumrit3, Jakrit Yaeram4, Chompunut Lumsangkul5
...earch study investigated cysteine supplements and their effects upon in vitro maturation (IVM) media. Experimentally, cumulus-oocyte complexes (COCs) which were obtained from bovine ovaries underwent culturing for a period of 22 h in TCM-199 containing an additional 10% fetal bovine serum, streptomycin (100 μg/ml), penicillin (100 U/ml), 25 μg/ml FSH, 2 IU/ml hCG, 0.2 mM sodium pyruvate, 1 μg/ml 17-β-Estradiol, and either 0, 100, or 200 μM/ml ...
Seyedmohammad Karimi1, Mohammad Darvishi2*, Mohammad Barati3, Ramin Hamidi Frahani4, Mohammad Hassan Kazemi5
...r. Melatonin and N-acetylcysteine, two drugs that have previously been shown to protect the liver with their antioxidant properties, may reduce the hepatic toxicity induced by Remdesivir. Given that few studies have been performed on the role of oral melatonin and N-acetylcysteine on reducing the hepatic adverse effects of Remdesivir, we decided to conduct this clinical trial study. In this double-blind, randomized clinical ...
Nida Sadaqat1, Sheheyar Ahmed Khan1, Amna Bibi1, Sadaf Zahra1, Muhammad faisal Salamt1, Noreen Latief2 and Fatima Ali1*
...e the effect of N-acetyl cysteine (NAC) on biochemical alterations during the post-burn stage in scalded rats. Twenty-four male Sprague-Dawley rats were anesthetized and dorsum was shaved. Second-degree skin burn was induced by immersing an iron mold (1.0 cm2 in diameter) in boiling (100°C) water for 5 min and placed on the back of the rats for 20 sec without applying pressure. Rats were divided into three groups; control group, burn injury group, burn inj...
Fatima Ali1*, Rimsha Tahir1 and Tahir Rehman Samiullah2
...ombination with N-acetyl cysteine (NAC) for the treatment of excisional wounds in rats. Sprague Dawley (SD) rats were inflicted with excisional wounds. Wounds were treated with honey as control group and in combinations with 1%, 3% and 5% NAC. Wound area was measured after 3rd, 7th, 10th and 14th day. Improvement in wound healing was also confirmed by analyzing angiogenesis, apoptosis and chemotaxis genes. A significant increase was observed in the percentage ...
Fariha Qahar and Muhammad Sayyar Khan*
...rate-limiting enzyme for cysteine (Cys), and GSH biosynthesis, were subjected to H2O2, metolachlor, and atrazine-induced oxidative stress. The overexpression of the NtSAT4 gene from Nicotiana tobacco under 35S promoters in various compartments of the cell, which includes cytosol, plastids, and mitochondria in transgenic lines, resulted in enhanced tolerance in terms of lesser wilting and pigment discoloration to induced stress compared to non-transformed plant...
Fariha Javaid1*, Zahoor Qadir Samra1, Madeeha Shahzad Lodhi1,2, Aroosha Hussain1 and Gulnaz Pervaiz1
...in is a thiol-containing cysteine protease isolated from the stem, peel, and leaf parts of Ananas comosus. Protease accounts for 60% of the total enzyme market. Being a plant-based cysteine protease, it has various pharmaceutical and biotechnological applications. The isolation and purification cost of the enzyme is highest, so there is a need to develop cost-effective purification methods. This research work aims to purify ...
