Issue · 2025
Pakistan J. Zool., Vol. 57, Iss. 4, pp. 1503-2002
62 articles · pp. 1503-2002 · All issues →
Research ArticleEffect of Dried Orange (Citrus sinensis) Pulp on Growth Performance, Serum Biochemical Parameters, and Nutrient Digestibility in Broiler Chickens
Najam ul Saqib, Ziaul Islam, Asad Sultan, Muhammad Shuaib, Ibiwoye Demilade, Shahrood Ahmed Siddiqui et al.
This study investigated the effect of dried orange pulp on growth performance, immunity, serum biochemical parameters, and nutrient utilization in broiler chickens. Two hundred and forty straight-run broiler chicks (Cobb 500) were allocated to 4 groups with 5…
30 May 2025DOI 10.17582/journal.pjz/20230803122156
Research ArticleEffects of Moringa oleifera on Growth Biomarkers, Carcass Characteristics and Hematological Parameters of Broiler Chicken
Sana Ullah, Faiqah Ramzan, Muhammad Shoaib Khan, Shakeeb Ullah
Antibiotics are frequently employed for promoting growth and preventing diseases, yet their application entails drawbacks including residual effects, drug toxicity, and the rise of bacterial resistance. The objective of the present study was to examine the imp…
2 Jul 2025DOI 10.17582/journal.pjz/20231206100720
Research ArticleImpact of Different Intensities of Green-Red Light During Incubation on Embryonic Index and Hatching Performance in Chicken
Muhammad Faisal Riaz, Athar Mahmud, Jibran Hussain, Saima
The poultry industry artificially incubates eggs by providing all constituents of natural incubation except light. However, in the last decade, many studies have provided enough proof that photo-stimulation during incubation has a positive impact on embryonic…
4 Jun 2025DOI 10.17582/journal.pjz/20231228215710
Research ArticleGenomic Study of Silent Heat: A Novel Mutation in HSD17β1 Gene Muting the Potential of Black Gold of Asia
Basit Imtiaz, Asif Nadeem, Huma Mujahid, Maryam Javed
Buffaloes are useful in the conversion of straw into agro-industry waste and bio-fertilizer, in addition to giving milk, meat, and mechanical power to humans. Between 65 and 60% of the world’s milk is produced by buffaloes. Regardless of their excellent…
13 Jun 2025DOI 10.17582/journal.pjz/20220817040832- Research Article
Effects of Calcium Supplementation on Blood Metabolites, Subclinical Mastitis and Reproductive Efficiency in Poor Performing Postpartum Dairy Cows
Shah Murad Khan, Rifat Ullah Khan, Hamayun Khan, Sarzamin Khan
Calcium supplementation is anticipated to positively impact reproductive performance, potentially enhancing fertility rates and outcomes. The current study aimed to assess the effects of varied calcium supplementation levels on milk yield, blood metabolites, u…
5 Jul 2025DOI 10.17582/journal.pjz/20240203053322 
Research ArticleEffect of Caffeine on the Testosterone and Cortisol Levels of University Football Players
Badar Mohy ud Din, Muhammad Zafar Iqbal Butt, Yasmeen Tabassum, Muhammad Amir Iqbal
This current study investigates the effect of caffeine on the testosterone and cortisol levels of university football players. For this purpose 120 university-level male football players (age: 22.39 ± 1.69 years; height: 172.9 ± 5.85 cm; body mas…
5 Jul 2025DOI 10.17582/journal.pjz/20230530150540
Research ArticleMolecular Epidemiology of Theileria lestoquardi in Small Ruminants in District Charsadda, Khyber Pakhtunkhwa, Pakistan
Shahab-ud-Din, Mashal Khan, Shad Muhammad, Said Sajjad Ali Shah, Hameed Ullah, Arab Hussain et al.
Small ruminant theileriosis is an economically significant disease in tropical and subtropical countries caused by Theileria lestoquardi, Theileria ovis, and Theileria luwenshuni, of which T. lestoquardi is very pathogenic. The goal of the current study was to…
2 Jul 2025DOI 10.17582/journal.pjz/20231109090656
Research ArticleIn vivo Anti-hyperlipidemic Potential of Chloroform Extract of Fagonia indica in Streptozotocin-induced Rats
Atiq-ur-Rehman, Sana Ullah Umair, Irfan Raza, Qudsia Kanwal, Bisma Mushtaq, Irfan ul Haq
The plant Fagonia indica is used in the treatment of various diseases. The purpose of this study was to elucidate the in vivo anti-hyperglycemic and antihyperlipidemic activity of chloroform extract of this plant in streptozotocin (STZ) induced diabetic rats.…
2 Jul 2025DOI 10.17582/journal.pjz/20230825095504
Research ArticlePolymorphic Analysis of the GH Gene (Exon 3 and 4) and its Association with Growth Traits in Pakistani Quail (Coturnix japonica PK)
Memoona Adil, Jibran Hussain, Sehrish Firyal, Saadat Ali, Zaka Ur Rehman, Muhammad Tayyab et al.
Genetic markers, such as single nucleotide polymorphisms (SNPs) in growth hormone (GH) genes, can play a significant role in selection for improving growth traits in poultry birds. The data on SNPs of GH genes of C. japonica in Pakistan is limited. Therefore,…
2 Jul 2025DOI 10.17582/journal.pjz/20230731171437- Research Article
Effect of L-Carnitine Supplementation on Oxidative Stress, Metabolic and Hormonal Profile in Pregnant Rabbit Doe
Chahat Ali, Hamayun khan, Rifat Ullah Khan, Muhammad Shuaib, Shahzada Khurram Adrian Shah, Madiha Hassan et al.
Several variables, such as hematology and hormone profile, influence the physiological state of pregnancy and throughout the various phases of pregnancy, the female reproductive system needs energy. Fetal health and production are negatively impacted by the bo…
1 Jul 2025DOI 10.17582/journal.pjz/20240205133249 
Research ArticleEffects of Ecological Factors on Occupancy of Artificial Nest Boxes by the Great Tit (Parus major): A Comprehensive Analysis
Dong-Ho Lee, Tae-Kyung Eom, Jae-Kang Lee, Hyeongyu Ko, Joo-Hee Kim, Sung-Hyun Ahn et al.
This study was conducted to clarify the ecological factors influencing the occupation of artificial nest boxes by the great tits (Parus major) in temperate mixed forests of South Korea. The occupation was determined based on the presence of the eggs and not by…
21 Jun 2025DOI 10.17582/journal.pjz/20240117094114- Research Article
Serological identification of Toxoplasma gondii in Goats and their Female Possessors in Kurram District of Khyber Pakhtunkhwa
Nabeela Shaukat, Mohsin Shah, Muhammad Imran, Fatima Muccee, Sher Zaman Safi, Shahrood Ahmed Siddiqui et al.
Toxoplasma gondii is a protozoan parasite that causes toxoplasmosis in animals. The present study was conducted to investigate the percentage of toxoplasmosis in goats and their female possessors in the Kurram district Pakistan. A total of 200 blood samples (1…
14 Jun 2025DOI 10.17582/journal.pjz/20230724074505 
Research ArticleImpact of Seasonal Variation on the Growth Dynamics of the Marine Catfish, Arius maculatus (Siluriformes: Ariidae) Collected from Karachi Fish Harbour
Muhammad Amin, Mohammad Shoaib, Naveed Ahmad, Mohammad Attaullah, Ayaz Ali Khan, Hazrat Ali et al.
Overfishing is the main cause of fish stock exploitation in the marine environment. In the current study growth dynamics of the marine catfish, Arius maculatus collected from Karachi Fish Harbour were studied. The relationship between total length and weight (…
8 Jun 2025DOI 10.17582/journal.pjz/20231125071011- Research Article
Influence of Cannon Bone Circumference on Some Body Size Traits in Harnai Sheep Breed of Balochistan
Bakhtawar Korai, Hubdar Ali Kaleri, Shahrukh Khan Korai, Zeenat Korai, Safeer Ahmed, Zaheer Ahmed Nizamani et al.
The excessive increase in demand for small ruminant meat is a result of both population growth and depletion of natural resources. One of the most significant economic characteristics that enhances an animal’s capacity to withstand excessive body weight,…
3 Jun 2025DOI 10.17582/journal.pjz/20231128090338 
Research ArticleThe Use of Genetic Merit and Allelic Frequencies to Alter bTB Susceptibility and Disease Progression in Cow Herds
Bejaoui Safa, Ferchichi Mohamed Amine, Jemmali Bayrem
The study aimed to characterize the relationship between Holstein cows’ TLR2 gene polymorphisms and their susceptibility to tuberculosis. In total, 200 Tunisian Holstein cows were used, in wich 100 control and 100 cases. PCR-RFLP and direct sequencing PC…
3 Jun 2025DOI 10.17582/journal.pjz/20240205022049
Research ArticleSerological Evidence of Chlamydiosis, Q Fever and Brucellosis in District Dadu, Pakistan
Hubdar Ali Kolachi, Shahid Hussain Abro, Asghar Ali Kambhoh, Saeed Ahmed Soomro, Nazeer Hussian Kalhoro, Abdul Ahad Soomro
Brucella melitensis, Chlamydophila (=Chlamydia) abortus and Coxiella burnetii are common abortifacient bacterial pathogens in sheep and goats. The establishment of active animal surveillance is essential to detect incidences as outbreaks of infections in small…
30 May 2025DOI 10.17582/journal.pjz/20210324160335
Research ArticleKey Genes Associated with Nonalcoholic Fatty Liver Disease and Colorectal Cancer in Experimental Animal Models
Fan Zhang, Shuai Han, Weijie Song
The objective of this study was to examine the differentially expressed genes between sick and normal tissues in animal models of nonalcoholic fatty liver disease (NAFLD) and colorectal cancer (CRC) based on the gene expression database (GEO) and to study the…
30 May 2025DOI 10.17582/journal.pjz/20230427020444
Research ArticleStudy on Hemato-biochemical Alterations, Oxidative Stress and Epidemiology of Parasitic Infestation along with Therapeutic Effect of Eprinomectin in Sheep in South Punjab
Muhammad Ali Raza, Riaz Ahmed Gul, Ali Raza, Khalid Mehmood, Abdul Qayyum, Muhammad Zahid et al.
Livestock plays a pivotal role in the rural economy because almost 35-40 million population in the villages are getting 30-40% of their income from livestock resources. The main health concern of sheep is parasitic infestation, which causes a decrease in weigh…
3 Jun 2025DOI 10.17582/journal.pjz/20231117063624- Research Article
Milk Yield, Composition and Physicochemical Properties of Indigenous and F1 Crossbred (Bos indicus with Bos taurus) Cows in Azad Jammu and Kashmir
Beenish Shahid, Ramla Komal, Fayzan Sultan Awan, Muhammad Ijaz Khan
Milk composition is a fiercely disputed topic among the nutrition scientists, so it’s crucial to know if crossbreeding of indigenous cows has an impact on milk composition. The present study was conducted to evaluate and compare the milk yield, physicoch…
14 Jun 2025DOI 10.17582/journal.pjz/20231004062217 
Research ArticlePutative Remedial Effect of Zinc Oxide Nanoparticles on the Apoptotic Response in Nile Tilapia Exposed to Potential Micro Plastics in Sewage Effluent
May Al-Bariqi, Promy Virk, Manal Ahmed Awad, Manal Qayyadh Saad Alanazi, Khalid Ortashi
Microplastics (MPs) are an emerging class of ubiquitous pollutants that pose a threat to aquatic biota. It has been established that Sewage treatment plant (STP) effluents are a potential source of MPs into the aquatic compartment of the environment. The prese…
8 Jun 2025DOI 10.17582/journal.pjz/20240126193405
Research ArticleAging and Infection-Based Alterations in Collagen Type 1 of Nile Tilapia (Oreochromis niloticus L.) Skin
Hossam I. Kadira, Ibrahim I. Al-Hawary, Abdallah Salah, Zizy I. Elbialy
Collagen is the amplest animal protein polymer and makes up about 30% of the total protein in the animal body. Collagen extracted from fish waste is not associated with serious infectious diseases nor is there religious objection. Here, we investigated the col…
14 Jun 2025DOI 10.17582/journal.pjz/20220925230911- Research Article
Biochemical Assessment of Oxidative Status and Liver Biomarkers in Acetaminophen Associated Hepatic-Toxicity in Broiler Chicks
Muhammad Mushtaq, Muhammad Shuaib, Muhammad Abdullah, Aamir Iqbal, Abubakar Sufyan, Muhammad Ismail Khan et al.
The present study was designed to evaluate the antioxidant activity of the methanolic extract of coriander seeds extract in broiler chicks during induced hepato-toxicity through acetaminophen. A total of 300 broilers (Cobb) chicks were used as tested criteria.…
30 May 2025DOI 10.17582/journal.pjz/20230613050644 
Research ArticleComparative Morphology and Morphometry of the Olfactory Organ of the Six Korean Spined Loaches, Genus Iksookimia, as a New Taxonomic Key
Seung Woon Yun, Jong Young Park, Hyun Tae Kim
The olfactory structure of Korean spined loaches, Iksookimia koreensis, I. pumila, I. pacifica, I. longicorpus, I. hugowolfeldi, I. yongdokensis, were investigated by anatomical observation and statistical analysis. They share the same olfactory morphology cha…
4 Jun 2025DOI 10.17582/journal.pjz/20230830034720
Research ArticleWhole Genome Sequence and In Silico Analysis of Probiotic Potential of Limosilactobacillus fermentum PC-10 and PC-76 Isolated from Poultry Gut
Adnan Mehmood, Muhammad Nawaz, Masood Rabbani, Muhammad Hassan Mushtaq
The Limosilactobacillus fermentum (PC-10 and PC-76) originally isolated from poultry gut have displayed antibacterial activity against Salmonella Gallinarum and potential in vitro probiotic properties. The aim of this study was to elucidate the genetic traits…
8 Jun 2025DOI 10.17582/journal.pjz/20230818034435
Research ArticleIn-Vitro and In-Vivo Antibacterial Effects of Hydroxamic Acid in Broilers
Muhammad Adnan Shams, Sarzamin Khan, Muhammad Shuaib, Muhammad Mushtaq, Najma Shams, Muhammad Aamir et al.
This study investigated the antibacterial efficacy of hydroxamic acid extracted from Azam maize root powder. The methanolic extract was tested for the availability of hydroxamic acid as an antibacterial agent through In-Vitro and In-Vivo tests in locally isola…
14 Jun 2025DOI 10.17582/journal.pjz/20231009121321
Research ArticleConstruction, Characterization, and Biosensing Potential of an SOS Inducible mCherry Based Reporter Plasmid
Shaista Bano, Sarfraz A. Tunio
Improved variants of mushroom coral derived monomeric red fluorescent protein have been considered as a reliable reporter protein for gene expression. One of these variants is the mCherry protein which is encoded by the mCherry gene. The aim of the present stu…
14 Jun 2025DOI 10.17582/journal.pjz/20230403060422
Research ArticleAn Annotated Checklist of Ichthyofaunal Diversity of the Godavari River, Andhra Pradesh, India
N. Veerabhadra Rao, P. Jawahar, N. Jayakumar, N.V. Sujathkumar, V. Rani, Ramesh Rathod et al.
A thorough examination of Ichthyofaunal diversity was carried out in the Godavari River in Andhra Pradesh from February 2022 to July 2023. The samples were identified up to the species level using standard taxonomic approaches such as morphometric, meristic, a…
22 Jun 2025DOI 10.17582/journal.pjz/20230820044600
Research ArticleMolecular Epidemiology of Enteric Pathogens Associated with Diarrheal Disease in Goat Kids
Maria Azmat, Jawaria Ali Khan, Muhammad Ijaz, Kamran Ashraf, Omer Naseer
Neonatal diarrhea is one of the leading cause of mortalities in goats leading to economic losses. The current research was conducted to study the prevalence of Salmonella and Escherichia coli in diarrheic goat kids. Fecal samples of 200 diarrheic goats up to t…
2 Jul 2025DOI 10.17582/journal.pjz/20231005073531- Research Article
Effect of Marinades on Quality Characteristics of Spent Hen Meat
Rashid Ali Korejo, Atta Hussain Shah, Muneer Ahmed Jamali, Tarique Ahmed Khokhar, Gul Bahar Khaskheli, Ghulam Shabir Barham et al.
This study investigates the influence of marinades on the quality attributes of spent hen meat. Spent hen meat, while a valuable protein source, often faces challenges related to tenderness and palatability. The study focuses on effect of marination on essenti…
5 Jul 2025DOI 10.17582/journal.pjz/20231127052631 
Short CommunicationCharacteristics and Temporal Patterns of Testes and Ovaries Development in the Hybrid Snakehead of Channa argus (♂) × Channa maculate (♀)
Aiguo Zhou, Shaolin Xie, Yanfeng Chen, Huijuan Tang, Jixing Zou
This study presents histological observations of hybrid snakehead ovaries and testes at different stages, as well as ultrastructural observations of sperm. The ovaries of hybrid snakehead exhibit a batch asynchronous type of development from stage III to stage…
6 Jul 2025DOI 10.17582/journal.pjz/20240114032649- Research Article
Prevalence, Awareness, and the Response of the General Population Towards Covid-19 in 2023
Abdul Rauf, Salma Farooq, Syed Ayaz Kazmi, Abdul Razzaq Ahmed, Muhammad Zahid Latif, Mohammed Merae Alshahrani et al.
The COVID-19 pandemic, initiated by the SARS CoV-2 virus, originated in Wuhan, China, in December 2019 and swiftly transcended international borders, impacting nearly every nation. While Pakistan faced significant challenges due to the virus, the country effec…
30 May 2025DOI 10.17582/journal.pjz/20231104200036 - Research Article
Prevalence, Awareness, and the Response of the General Population Towards Covid-19 in 2023
Abdul Rauf, Salma Farooq, Syed Ayaz Kazmi, Abdul Razzaq Ahmed, Muhammad Zahid Latif, Mohammed Merae Alshahrani et al.
The COVID-19 pandemic, initiated by the SARS CoV-2 virus, originated in Wuhan, China, in December 2019 and swiftly transcended international borders, impacting nearly every nation. While Pakistan faced significant challenges due to the virus, the country effec…
30 May 2025DOI 10.17582/journal.pjz/20231104200036 - Research Article
Prevalence, Awareness, and the Response of the General Population Towards Covid-19 in 2023
Abdul Rauf, Salma Farooq, Syed Ayaz Kazmi, Abdul Razzaq Ahmed, Muhammad Zahid Latif, Mohammed Merae Alshahrani et al.
The COVID-19 pandemic, initiated by the SARS CoV-2 virus, originated in Wuhan, China, in December 2019 and swiftly transcended international borders, impacting nearly every nation. While Pakistan faced significant challenges due to the virus, the country effec…
30 May 2025DOI 10.17582/journal.pjz/20231104200036 
Research ArticleStudies on the Identification of Variants in Ribosomal RNA Genes in Maternal Diabetic Families: A Combined Approach Involving Nucleotide Sequence Analysis and Bioinformatics
Sajid ul Ghafoor, Aziz-ud-Din, Khushi Muhammad, Inamullah, Abid ul Ghafoor
The MT-RNR2 gene plays a crucial role in encoding 16S ribosomal RNA, which is essential for the mitoribosomal larger subunit (mt-LSU). Variations in this gene have the potential to disrupt the secondary structure of 16S rRNA, affecting the assembly of mt-LSU n…
13 Jun 2025DOI 10.17582/journal.pjz/20240123131923
Research ArticleCombined Effect of Decellularized Rat Brain Tissue and Nerve Growth Factor on 5 Neural Differentiation of PC12 Cell Line in 2D Cell Culture
Seyed Majid Jalalian Hosseini, Javad Baharara, Shahrbanoo Oryan, Mohammad Amin Kerachian
Recent advancements in tissue engineering suggest that biomaterials, particularly decellularized extracellular matrix (ECM), have the potential to improve the targeting and effectiveness of regenerative therapies in the central nervous system. In present exper…
21 Jun 2025DOI 10.17582/journal.pjz/20240213050000
Research ArticleDetection System of Compound Amplification of Autosome and Y Chromosome Loci: Establishment and Practical Application
Fang Si, Longfei Ma, Xiangyang Liu
The objective of this study was to establish a new compound amplification detection system by using the loci of autosomes and Y chromosomes to achieve the classification of DNA, so as to identify forensic samples. A total of two hundred fifty blood samples wer…
2 Jul 2025DOI 10.17582/journal.pjz/20240101170030
Research ArticleProduction of an Asparaginase-Like Domain of AnsA from Methanocaldococcus jannaschii in Escherichia coli, its purification and In Silico Analysis
Maham Ijaz, Mohsin Shad, Arshia Nazir, Naseema Azim, Muhammad Sajjad
L-asparaginase (E.C 3.5.1.1) is a highly valuable enzyme owing to its ability to function as a chemotherapeutic agent against certain lymphomas such as acute lymphoblastic leukemia. This enzyme also mitigates the level of acrylamide production in processed foo…
2 Jul 2025DOI 10.17582/journal.pjz/20231025191403
Research ArticleHisto-morphometric Indices and Catalase Response in Muscles, Gills and Intestine of Cadmium Stressed Hypophthalmichthys molitrix
Rida Ajmaal, Huma Naz, Khalid Mehmood, Moazama Batool, Najeeb Ur Rehman, Tanveer Ahmed et al.
The experiment was done to assess at the impact of cadmium (Cd) on histology and antioxidant enzyme (catalase) activity in different tissues (gills, intestine, and muscles) of freshwater fish, Hypophthalmichthys molitrix. For this purpose, acute toxicity of Cd…
5 Jul 2025DOI 10.17582/journal.pjz/20230509070520
Research ArticleEffect of Dietary Solanum nigrum Extract Supplementation on Growth Performance, Nutrient Digestibility, and Anti-Oxidant Activity in Labeo rohita Fingerlings
Muhammad Arshad, Syed Makhdoom Hussain, Li Jiali, Majid Hussain, Zeeshan Yousaf
This study aimed to investigate the effect of Solanum nigrum extract (SNE) supplementation on the growth performance, nutrient digestibility, and antioxidant status of Labeo rohita fingerlings fed soybean meal diet. The fish were fed a formulated diet with var…
13 Jun 2025DOI 10.17582/journal.pjz/20230725204942- Research Article
Impact of Supplementation of Poultry Feed with Locally Characterized Recombinant Thermostable Xylanase on the Growth Performance of Broiler Chicks
Asma Waris, Ali Raza Awan, Sehrish Firyal, Naeem Rashid, Abu Seed Hashmi, Muhammad Wasim et al.
The current study evaluated the efficiency of locally characterized recombinant thermostable xylanase (XYLTN) from Thermotoga naphthophila in broiler chicks. The XYLTN was produced using BL21 Codon Plus (DE3) cells having pET-21a containing xylanase gene from…
8 Jun 2025DOI 10.17582/journal.pjz/20231024054103 
Research ArticleDiversity of Butterfly Fauna in Potohar Plateau, Pakistan
Waheed Iqbal, Muhammad Faheem Malik
An investigation was carried out to study the diversity of butterfly fauna at the 53 selected locales of Potohar Plateau, Pakistan during July 2017 to September, 2019. During this study, 32 species of butterflies were identified out of 1117 specimens that belo…
8 Jun 2025DOI 10.17582/journal.pjz/20231217050506
Research ArticleEvaluation of Ocimum basilicum and Moringa oleifera as a Natural Preservatives of Canned Tuna Product
Ghada A.K. Kirrella, Nader Y. Moustafa, Nancy Nagy, Reda Abdallah
This study was conducted to evaluate the quality of local canned tuna products sold in supermarkets in Kafr El Sheikh governorate, through physicochemical, bacteriological and sensory parameters assessment in an attempt to improve the quality of local products…
13 Jun 2025DOI 10.17582/journal.pjz/20220802150853
Research ArticleComparison of Floored and Cage Housed Broiler Breeder Farms for Energy and Economic Efficiency in Pakistan: A Case Study of Broiler Breeder’s Farm in District Punjab
Ishtiaq Ahmad Khan, Sarzamin Khan, Muhammad Shuaib, Sohaib Ul Hassan, Abdulmohsen Hussen Alqhtani, Anthony Pokoo-Aikins et al.
Research trials were conducted at commercial broiler breeder farms (n=60,000) to compare the economic and energy efficiency of floored and caged housing systems. Efficiency was evaluated by the inputs and outputs used. The egg prices were as per market rates a…
13 Jun 2025DOI 10.17582/journal.pjz/20230810114347
Research ArticleGenetic Diversity and Molecular Characterization of Virulence Determinants and Integrons in Pseudomonas aeruginosa Isolated from Canal Water in Peshawar Pakistan
Urooj Amjad, Kafeel Ahmad, Hifsa Saadia, Imran Muhammad Khan
Pseudomonas aeruginosa is a clinically significant Gram-negative bacillus with remarkable ability to thrive in diverse environments that could be attributed to its minimal nutritional demands allowing it to exist in settings like wastewater, surface water, and…
22 Jun 2025DOI 10.17582/journal.pjz/20231124100835
Research ArticleRhodiola rosea Stimulates Brain Microcirculation in Rats with Hypoxic Brain Injury at High Altitude
Qiang Chen, Chengshuang Yang, Daojin Wang, Sha Luo, Siqing Ma
Acute hypobaric and hypoxic environment can reduce the activity of peroxidase in brain tissue and increase the accumulation of peroxide products, so as to enhance the risk of cerebral microcirculation disorders. Rhodiola rosea (RR) is a classic prescription of…
2 Jul 2025DOI 10.17582/journal.pjz/20240206130209
Research ArticleMolecular Identification and Genome-Wide Analysis of a New Strain of Porcine Parvovirus Type 4 in Northwestern Sichuan, China
Miao Yin, Xi-wen Chen, Xing-Yan Tong, Yu-Lu Yang, Xiao-Yue Tang, Cong Wang et al.
The aim of this study was to understand the infection status and full sequence characteristics of porcine parvovirus type 4 (PPV4) in pig herds in a breeding farm in north western Sichuan, China. Two hundred ninety-six serum samples from the breeding farm were…
2 Jul 2025DOI 10.17582/journal.pjz/20221118131127- Short Communication
Polymorphism in CD40 Gene Associated with the Prevalence of Rheumatoid Arthritis in Pakistan
Naila Riaz, Muhammad Arshad, Faiza Zubair
The polymorphism in CD40 gene have been associated with rheumatoid arthritis (RA). A single SNP rs1535045 was genotyped in 100 RA patients and 100 healthy controls. Some risk factors linked with RA like hypertension, diabetes and smoking were also assessed in…
6 Jul 2025DOI 10.17582/journal.pjz/20191230091215 
Short CommunicationMolecular Characterization and Genetic Identification of Pakistani Partridges Through DNA-Barcoding
Muhammad Zia ul Haq, Asim Iqbal, Rana Manzoor Ahmad, Aftab Ahmad Anjum, Aqsa Arshad, Muhammad Wasim et al.
Now a days, DNA barcoding using COI gene (mitochondrial cytochrome c oxidase subunit I) has gained more attention, because of these barcodes validity and simplicity for identification of bird species has increased a lot. In present study, 650bp region of COI g…
6 Jul 2025DOI 10.17582/journal.pjz/20230630100651
Research ArticleBrevilin A Binds with Neh1 Domain of NRF2 and Inhibits its Translocation into Nucleus in Cancer Cells
Sameena Gul, Muhammad Akhtar Ali, Muhammad Khan
Nuclear factor erythroid 2-related factor 2 (NRF2) is a transcriptional factor which is constitutively activated in various cancers where it confer survival advantages by transcribing cytoprotective genes involved in cancer initiation, progression, metastasis…
5 Jul 2025DOI 10.17582/journal.pjz/20240403042208
Research ArticleEffect of Feeding Different Milk Types on Pre-Weaning Performance of Kundi Buffalo Calves in Pakistan
Hassan Mahmood Warriach, Niaz Ali Kaka, Amir Amanullah Solangi, Aijaz Kumbher, Sobia Majeed, Khizar Hayat et al.
Rearing buffalo calves with a low-cost feeding regime remains a major challenge in many buffalo production systems of the world. The current study aimed to evaluate the effect of feeding different milk types on pre-weaning performance of Kundi buffalo calves i…
5 Jul 2025DOI 10.17582/journal.pjz/MH20230213070202
Short CommunicationEffect of Supplementation of Papaya Seed (Carica papaya) on Growth Performance, Carcass Traits, and Histomorphology of Japanese Quails
Irfan Ullah, Rafi Ullah, Muhammad Shuaib, Sohaib Ul Hassan, Abdulmohsen Hussen Alqhtani, Anthony Pokoo-Aikins et al.
This study was conducted to examine the effect of papaya seed on growth performance, gut morphometry, and economics in Japanese quail. Two hundred and twenty day-old quail chicks were used for the experimental purposes and were divided into four groups each wi…
6 Jul 2025DOI 10.17582/journal.pjz/20230824082655
Short CommunicationComparative Analysis Reveals Recent Mitochondrial Introgression and Genome Size Variation in the Yellow Goosefish Lophius litulon
Jing Luo, Yaoren Zhang, Jun Yang, Shengyong Xu
Uncovering intraspecific genetic differentiation is important for evolutionary and phylogenetic investigations of marine fish species. Compared with partial mitochondrial sequences, the mitochondrial genome (mitogenome) is more informative, providing higher re…
6 Jul 2025DOI 10.17582/journal.pjz/20240123020753
Research ArticleEffects of Antibiotic Treated Mulberry Leaves Feeding on Larval Growth, Cocoon Production, and Gut Bacteria of Silkworm
Areeba Mashaal, Mubashar Hussain, Aleena Naeem, Kiran Aftab, Ghulam Dastgeer, Shumaila Irum et al.
The effect of different concentrations of Levofloxacin and Cefixime on bacterial profile, larval growth, and cocoon production of silkworms was evaluated by rearing under controlled conditions of temperature, relative humidity, and photoperiod (25 ± 1 &…
14 Jun 2025DOI 10.17582/journal.pjz/20231201085224- Research Article
The Effects of Brewer’s Yeast on Growth Performance, Rumen Fermentation, and Serum Antioxidant Markers in Beef Cattle
Mingming Jiang, Yanling Zheng, Daoping Ren, Qudrat Ullah, Muhammad Zahoor Khan, Yulin Ma
This study aimed to investigate the impact of brewer’s yeast supplementation on the productive performance, rumen fermentation, and serum antioxidant capacity of Simmental × Charolais crossbred beef cattle. Forty healthy beef cattle with same body…
30 May 2025DOI 10.17582/journal.pjz/20240110151005 
Research ArticleNesting Distribution and Conservation of the Brown Booby (Sula leucogaster) on the Red Sea Coast of Saudi Arabia
Mohammed Almalki
Brown boobies (Sula leucogaster) have a wide range throughout the pantropical oceans, with a few exceptions. Even though this species has an extensive distribution range, little is known about its breeding distribution in the Red Sea region. Brown booby popula…
4 Jun 2025DOI 10.17582/journal.pjz/20230926145030
Research ArticleCow Comfort Levels and Milk Production of Holstein Cows in Cool and Cold Seasons
Coskun Aksu, Savas Atasever
Cow comfort is a crucial factor for both milk quality and quantity, as well as the health of the animal. This study aimed to determine the variation of comfort indicators and milk production during cool and cold seasons in dairy cows, as there is still a lack…
8 Jun 2025DOI 10.17582/journal.pjz/20240123101513
Research ArticleThe Attenuative Effects of Zinc Oxide Nanoparticles and S-Methylmethionine Sulfonium Chloride Against D-Galactose Induced Liver Oxidative Stress and Apoptosis in Albino Rats
Tarek Kamal Abouzed, Marwa F. Ezzeldeen, Azza Mansour Elkattawy, Ola A. Elbohy, Doaa Abdullah Dorghamm
D-galactose has been documented to induce a sign of aging and is linked to liver injury. Our study aimed to evaluate the protective effect of zinc oxide nanoparticles and S-Methylmethionine sulfonium chloride against D-galactose-induced liver injury in rats an…
13 Jun 2025DOI 10.17582/journal.pjz/20230523160530- Research Article
Effect of Aqueous Extract of Cyclotrichium niveum on the Lipophilic Vitamins, Cholesterol and Fatty Acids Profile in the Brain and Kidney of Streptozotocin-induced Diabetes in Rats
Mehmet Guvenc, Mustafa Topaloglu
This study examined the influence of Cyclotrichium niveum (CN) extract on fatty acids, ADEK vitamins, cholesterol, and malondialdehyde levels in the brain and kidney of streptozotocin (STZ)-induced diabetes in rats. Thirty-two adults female Wistar albino rats…
21 Jun 2025DOI 10.17582/journal.pjz/20221230101257 
Research ArticleA Model for Early Detection of Lumpy Skin Disease in Cattle Using Ensemble Technique
Saqib Ali Khaskheli, Muhammad Yaqoob Koondhar, Zulfikar Ahmed Maher, Gul Bahar Khaskheli, Azhar Ali Khaskheli
Lumpy skin disease in cattle is endemic disease showing a threat to overall livestock industries. In the past several traditional practices have been used to analyse these types of diseases such as poly chain reaction (PCR), clinical practices, laser, photonic…
21 Jun 2025DOI 10.17582/journal.pjz/20230810102300
Research ArticleMarek’s Disease in Chicken Flocks in JuRong, Jiangsu Province, China: Pathology, Virus Isolation, and Molecular Characteristics Analysis
Xue Lian, Hui Meng, Yuan Zhang, Cuihua He, Yongqian Pu, Shanfeng Wang et al.
Marek’s disease (MD), one of the most important avian immunosuppressive and neoplastic diseases, causes enormous economic losses in China. In 2023, seven 3-month-old chickens were determined to be infected with Marek’s disease virus (MDV) by histop…
1 Jul 2025DOI 10.17582/journal.pjz/20231224151430- Short Communication
Sexual Dimorphisms in Body Measurement Traits of Boer Goats in South Africa
Victoria Rankotsane Hlokoe, Motsatsi Surprise Ramoshaba, Thobela Louis Tyasi
Sexual dimorphism is the consequence of the combined sex-specific genes on the sex chromosomes and sex-specific gene expression in female and male animals. The study was conducted to investigate the influence of sex on body weight (BW) and linear body measurem…
6 Jul 2025DOI 10.17582/journal.pjz/20220317100341 - Research Article
Effect of Bio-Fermented Feed on Simmental Crossbred Cattle’s Growth Performance, Rumen Fermentation, and Antioxidant Status
Daoping Ren, Yiping Guo, Mingming Jiang, Yuxin Sha, Qudrat Ullah, Yulin Ma et al.
This study aimed to investigate the impact of microbial fermentation of crop by-product feed on the performance, rumen fermentation, and serum antioxidant properties of Simmental crossbred cattle. Forty healthy Simmental crossbred with similar body weights wer…
1 Jul 2025DOI 10.17582/journal.pjz/20230928085050