The Expression and Distribution of MIF, ERK, Akt and Rb/E2F in Pulmonary Hypertension in Broilers
The Expression and Distribution of MIF, ERK, Akt and Rb/E2F in Pulmonary Hypertension in Broilers
Yanmei Wang1,2, Haoyun Li1, Lijuan Han1 and Wenkui Wang1*
mRNA expression of MIF (A), Raf (B), ERK (C), Akt (D), GSK3β (E), cyclin D1 (F), Rb (G), E2F (H) in the lungs of PH broilers (n = 3, compared with healthy lungs, ** P <0.01). MIF: migration inhibition factor.
Western blotting analysis of protein expression in PH broilers lung. (a) The effect of PH broilers lung on MIF, Raf, ERK, Akt, GSK3β, cyclin D1, Rb, E2F protein expression. (b) Western blotting analysis of the test results: (n = 3, compared with healthy broilers lung, ** P <0.01).
Immunohistochemical analysis of protein in broiler lungs (magnification: 400×). a, MIF; b, p-Raf; c, p-ERK; d, p-Akt; e, p-GSK3β; f, cyclin D1; 1, negative control with PBS; 2, the lungs of healthy broilers; 3, the lungs of PH broilers.
