Hamayun Khan1,*, Momen Khan2, Muhammad Subhan Qureshi3, Shakoor Ahmad1, Ali Gohar4, Hameed Ullah5, FarmanUllah6, Arab Hussain7, Pershotam Khatri8, Said Sajjad Ali Shah1, Hamid Rehman1 and Azmatullah Khan1
...ality characteristics of post-thawed spermatozoa such as motility, viability and plasma membrane integrity. The data was statistically analyzed with one-way analysis of variance using SPSS. The highest significant (P<0.05) response was obtained in extenders containing 0.75% green tea extract for all studied parameters. Based on the findings of the current study, it can be concluded that supplementation of green tea extract in semen extender showed significa...
Alamaary Mohaammed Saad1,3, Abd Wahid Haron1*, Mohamed Ali2,
Mark Wen Han Hiew1 and Lawan Adamu1
...s used as a control. The post-thawed semen from all the samples were assessed for motility, oxidative stress, sperm membrane integrity, morphology, and acrosome integrity. Four stallions were nominated for AI in the current study. The best result from different concentrations of cysteine and ascorbic acid were used for AI. Thirty mares were divided into three groups of 10 mares each and were then inseminated using the frozen semen with the following extenders:...
Noor Bad Shah Khattak1, Hamayun Khan1, Muhammad Tarique Tunio2, Shakoor Ahmad1, Nazir Ahmad3, Syed Murtaza Hassan Andrabi4, Obaidullah5, Ihsan Ali1,5,* and Nan-Zhu Fang5,*
...(GT) extract on standard post-thawed quality indicators of frozen buffalo bull’s spermatozoa. Semen samples were obtained from the bulls for three weeks through artificial vagina. Egg yolk was used as an extender, in which different concentrations of GT extract (0.0%, 0.50%, 1.0%, 1.5%, 2.0% and 2.5%) were added. Semen quality indicators studied were the percentage of motility, survivability, plasma membrane integrity and acrosome integrity of frozen buf...
Ibrahim El-Ratel1*, Mostafa El-Moghazy2, Amira El-Gaml2, Ibrahim Abu El-Naser2
...ldehyde was decreased in post-thawed semen by all PEE levels. Conception rate of doe rabbits was improved by artificial insemination with fresh and thawed buck semen with all PEE levels, being the highest with PEE (300 mg/buck) and slightly higher for fresh than thawed semen. In conclusion, PEE oral administration (300 mg/buck) for 40 days may be used, as an effective tool, to improve antioxidants capacity, immunity, and quality and fertilizability of fresh an...
Sigit Bintara1*, Riyan Nugroho Aji1, Panjono2, Ali Agus3
Chairdin Dwi Nugraha1, Rafika Febriani Putri1, Ari Ardiantoro2, Wike Andre Septian1, Ahmad Furqon3, Kuswati Kuswati1, Nashi Widodo4, Suyadi Suyadi1*
Rizkie Elvania1, Tintin Rostini1, Sakiman2, Ani Susilawati2, Emilda Adriani2, Abd Malik1*
...e quality of spermatozoa post-thawed. Therefore, research was conducted on the use of lycopene as an effort to improve the quality of spermatozoa post-thawing. This study aimed to assess the impact of incorporating lycopene into both the skim milk and egg yolk extender at varying doses on pre-freeze and post-thawed Kalang buffalo semen. Four Kalang buffalo, aged between 6 to 7 years and weighing 500-550 kg, were involved in ...
Nawab Ali1, Akeel Ahmed Memon1, Asmatullah kaka1, Amjad Hussain Mirani2, Muhammad Ibrahim Panhwar3*, Nisar Ahmed Solangi1, Kashif Ali Malik1, Fazul U Rahman1, Moin Akhtar Vistro1
...tly (P<0.05) improved post-thawed motility (68.1±0.42), morphology (73.5±0.3), membrane integrity (70.8±0.36) and live dead ratio (77.1±0.41) were observed in group B as compared to other groups. For in vivo fertility assessment, twenty goats were selected and divided into two experimental groups, viz., group A and B (n=10/group). The goats of both groups were synchronized using the Ovsynch protocol. Group A goats were inseminate...
Atikah Nur Baity1, Noni Ashri Maghfiroh1, Syalsa Bella Fitriana1, Kurniawan Dwi Prihantoko2, Dyah Maharani2, Diah Tri Widayati2*
... on DNA fragmentation of post-thawed semen of Bali bull. This research was conducted at the Laboratory of Animal Physiology and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada. Seventy-five semen straws produced from one bull were divided into 3 groups; each group consisted of 25 semen straws as long as 3, 6, and 8 months of storage in liquid nitrogen. The quality of thawed semen included motility, viability, abnormality, plasma membrane integ...
Langgeng Priyanto1,2*, Herdis2, Santoso2, Pradita Iustitia Sitaresmi2,
Tri Puji Priyatno2, Rahma Isartina Anwar2, Desiana Ade Mahari2,
Florentina Bety Indah Lupitasari2, Maman Surachman2,
I Wayan Angga Darmawan2, Arfan Abrar1 and Arum Setiawan3
...asma membrane quality of post-thawed Tropical Brahman bull sperm. Sperm samples were obtained and pooled from eight sexually mature Tropical Brahman Bull, separated into seven equal proportions, and diluted with TEY extender Semen was supplemented with 0 (C0;E0), 5 (C5;E5), 10 (C10;E10), and 15 (C15;E15) mg/mL of each α-tocopherol and ascorbic acid, respectively. Total sperm motility, viability, abnormalities, and semen membrane plasma were analyzed afte...
Abdullah Channo1,2*, Asmatullah Kaka1, Akeel Ahmed Memon1, Mool Chand Malhi3, Muhammad Bakhsh4, Qudratullah Kalwar5, Shakeel Ahmed Tunio6 and Muhammad Ibrahim Panhwar7
...sed egg yolk extender on post-thawed quality of Tharparkar cattle bull semen. The research was carried out at Department of Animal Reproduction, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agricultural University Tandojam. In this study four fertile Tharparkar bulls (A, B, C and D) having age of 4-5 years were used. A total of 52 (n=13) ejaculates were collected with the help of artificial vagina, after the collection macroscopic (color, volume ...
