John G. Bruno
...eloped against extracted lipopolysaccharides (LPS) of E. coli O157 and the so called “Big six” non-O157 Shiga-toxin producing E. coli (STEC) serovars (O26, O45, O103, O111, O121 and O145). These aptamers were assessed by enzyme-linked aptamer sorbent assay (ELASA) to rank their relative affinities and discriminatory ability for distinguishing between each of the STEC serovars. The most specific biotinylated aptamers from the ELASA screening were th...
Zhenyu Chang1, Hui Zhang1, Khalid Mehmood1,2, Mujeeb Ur Rehman1, Xiaodan Yuan1, Zhaoyang Li1, Fazul Nabi1, Xiaoxing Wu1, Xinxin Tian1, Xueting Liu1, Jingen Xu3 and Donghai Zhou1,*
... cells were treated with lipopolysaccharide (LPS) and recombinant human (rh) TFF3. Cell proliferation and TFF3 mRNA expression were examined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assays and real-time quantitative PCR, respectively. The results showed that rhTFF3 effectively prevented the cell injury induced by LPS (P<0.05). A low concentration of LPS (10 µg/mL) down-regulated the expression of TFF3 mRNA, wherea...
Jun-Ying Liu, He-He Liu*, Jie Kou, Yang-Mei Zhao, Ji-Wen Wang, Liang Li and Xiao-Hui Du
...family and can recognize lipopolysaccharide (LPS), the main component of gram-negative bacteria. To investigate how TLR4 responds to LPS, the TLR4 transcription levels in duck liver, spleen and bursa of Fabricius were examined after a model for immune stress was implemented through LPS injection in Peking ducks. The results from this assay suggested that LPS injection significantly increased the concentration of tumor necrosis factor-α (TNF-&alpha...
Xiaojie Ding, Xiling Sun, Zuien Wang, Qiusheng Zheng, Xiaofei Yu, Wenjin Hao, Kejun Wang, Wenjuan Xu and Zhengping Dong*
...), interleukin-6 (IL-6), lipopolysaccharide (LPS) as well as colonic mucosal tissue TLR4/9 and NF-kB. We got the following results: (1) The rat IBS-D model was successfully prepared; (2) Wumei Pill could reduce the contents of serum TNF-α, IL-6 and LPS in rats(P<0.05), and alleviate the inflammatory reaction and accumulation of bacterial endotoxins; (3) Wumei Pill could reduce the transcriptional level of TLR4/9mRNA, to decrease TLR4/9 protein synthes...
Xiaopeng Tang1,2* and Kangning Xiong1,2
Ali Zaman1, Nabila Roohi1 and Muhammad Irfan2*
...nd its immunogens, i.e., lipopolysaccharide (LPS) and outer membrane protein (OMP) on Nili Ravi buffaloes. Prepubertal Nili Ravi female buffaloes (N=18) of 10-12 months age in good health condition were divided into five treatment groups (n=3 each) and a control group (n=3). Treatment groups’ animals were exposed to P. multocida bacterial culture and its immunogens, i.e., LPS (oral and intravenous) and OMP (oral and subcutaneous). Animals were analyzed f...
Yawang Sun, Yongjiang Wu, Jingbo Chen, Zili Wang, Juncai Chen, You Yang and Guozhong Dong*
...nt DNA damage induced by lipopolysaccharide (LPS) in bovine mammary epithelial cell line MAC-T cells. Cells were cultured for 48 h with different LPS levels (0, 0.1, 0.5, 2.5, 12.5, and 100 ng/mL). The results showed that cell viability was negatively correlated with LPS concentrations. The production of reactive oxygen species increased in a dose-depend manner. Cellular concentrations of oxidative damage markers were positively correlated with applied LPS con...
Zhiyuan Sun1, 3, Xuhui Zhang2,*, Xian Li3, Yanping Huang3, Shiyan Sui3, Weifeng Liu3, Guochao Ni3, Xiaozhou Xu1, Jianbo Yang1, Xue Lian1 and Hui Jia1
...r chronic stress affects lipopolysaccharide (LPS) -induced inflammation via the crosstalk. Pigs were administered LPS injection with or without continuous adrenocorticotropine hormone (ACTH) pretreatment. Twenty-four 30-day-old Duroc × Landrace × Large White crossbred piglets (12 ± 0.5 kg) were randomly assigned to 4 treatments: control, LPS, ACTH, and ACTH+LPS groups. Each group consisted of 6 male piglets. All ACTH pigs and ACTH+LPS pigs w...
Xiaopeng Tang1*, Kangning Xiong1 and Rejun Fang2*
...C-J2 cells challenged by lipopolysaccharide (LPS). Cells were treated with: (1) EGF (0 ng / mL) + LPS (0 μg / mL) (Control group), (2) EGF (100 ng / mL) + LPS (0 μg / mL) (EGF group), (3) EGF (0 ng / mL) + LPS (1 μg / mL) (LPS group), and (4) EGF (100 ng / mL) + LPS (1 μg / mL) (EGF+LPS group) for 24 h. The gene expression of glutamine transport protein Na+-dependent neutral amino acid transporter (ASCT2) and protein expression of ASCT2 were measur...
Wuritunashun*, Burenbatu, Narenqiqige, Jiuguniang, Eerdunduleng, Shuanglian Wang, Cuiqin Gong, Hashengaowa, Huizhi Jin, Baiwurihan and Chunhaizi
...tor VII, proteoglycan 4, lipopolysaccharide-binding protein (LBP), thrombospondin-4, and plasminogen activator inhibitor 1 were downregulated. Bioinformatics analysis of these differentially expressed proteins revealed that they play a critical role in immune-related responses. Parallel reaction monitoring validation also substantiated the results of the LFQ proteomics approach by analyzing the expression of LBP and SERPINA10. This work identifies the proteins...
Abdulaziz A. Alaqil*
...travenously injection of lipopolysaccharides (LPS). A total of one hundred twenty, 40-week-old, laying hens from the Hy-line breed were allocated at random into four groups of 30 hens each. For eight consecutive weeks (40–48 weeks of age), two groups of the layers were given a basal diet as control, while the remaining two groups were given a basal diet enhanced with a 2 g/kg diet of LA bacteria. At 47 wk of age, layers in one of the control or LA g...
Taidong Wang1, Xiaowei Huang1, Jian Huang2, Guangfu Lv3 and Zhe Lin1*
...an significantly inhibit lipopolysaccharide-induced fever in rats with elevated body temperature, reduce serum TNF-α, IL-1β and IL-6 levels, reduce the hypothalamus COX2, caspase3 and Bax protein expression levels and improve BCL2 protein expression levels, play an antipyretic effect....
Li Min1, Yong Chen2, Shunying Wu3, Xuefei Zheng1 and Mucheng Xu1*
...0 μ m/m and 1 μ of lipopolysaccharide (LPS) for 24 h. The ADPSCs of young rats were divided into groups A and B, respectively at 0 and 1 μ/mL of LPS for 24 h. The number of mineralized nodules and ALP activity in group B were significantly higher than those in group D. The protein expression of OCN and ALP in group B was significantly higher than that in group A. ALP protein expression in group D was significantly higher than that in group C. The prot...
Laila Ibouzine-Dine1*, Inssaf Berkiks1,2, Hasnaa Mallouk1, Mouloud Lamtai1, Zineb El Marzouki1, Amal Dimaoui1, Abdelhalem Mesfioui1, Aboubaker El Hessni1
... immune challenges, like lipopolysaccharide (LPS) exposure, can induce lasting changes in brain structure, neurochemistry, and function, a process known as “early-life programming.” These changes are associated with neuropsychiatric disorders, including depression. While the nitric oxide (NO) pathway is implicated in depression, how changes in NO kinetics impact sex-dependent affective behaviors has not been thoroughly investigated. This study focu...
