Abdul Nabi Jatt1,*, Sarfraz Ali Tunio1, Shaista Bano Memon1, Abdul Sattar Qureshi2 and Muhammad Aqeel Bhutto2
...ase, acidic-phosphatase, lipase, valine arylamidase, cystine arylamidase, napthol-AS-BI-phosphohydrolase, β-galactosidase, leucine arylamidase, and trypsin. While, esterase, esterase lipase, α-chymotrypsine, α-galactosidase, β-glucuronidase, α-glucosidase, β-glucosidase, N-acetyl- β-glucosaminidase, α-mannosidase, and α-fucosidase were recorded as negative. Moreover, S. dy...
Amna Waseem, Sikander Ali* and Syeda Wajiha Khalid
...duction of extracellular lipase (EC 3.1.1.3) from a Rhizopus oligosporus mutant strain developed by chemical mutagenesis. The wild-type was simultaneously treated with different concentration of EMS and MMS. Among 13 mutant variants, EM-7 showing maximum lipase activity of 71±3.55 U/g, was selected. Resistance in the hyper producing strain was developed by exposing it with low levels of L-cysteine HCl to...
Studies on amylase and lipase activity in fishes fed with diet containing different feed ingredients
Mirfa Murtaza1, Sajid Abdullah1, Wardah Hassan1*, Khalid Abbas1, Huma Naz1, Muhammad Anjum Zia2
Studies on amylase and lipase activity in fishes fed with diet containing different feed ingredients
... activity of amylase and lipase was higher in all fish species fed with corn gluten. Liver showed higher
amylase activity than the intestine, while the activity of lipase was higher in intestine. It was concluded that different
fish species respond differently to various feed ingredients. Therefore it is important to follow the acceptability and
digestibility standards during the selection of ingredients...
amylase activity than the intestine, while the activity of lipase was higher in intestine. It was concluded that different
fish species respond differently to various feed ingredients. Therefore it is important to follow the acceptability and
digestibility standards during the selection of ingredients...
Haniya Mazhar1, Naaz Abbas2, Zahid Hussain3, Amir Sohail4, Syed Shahid Ali4*
Extracellular lipase production from Bacillus subtilis using agro-industrial waste and fruit peels
...ed for the production of lipases by solid-state fermentation (SSF) using agro-industrial wastes as substrate. A total of 14 bacterial strains were isolated, screened and compared for lipase production. Among the evaluated strains, Bacillus subtilis strain NL-39 was selected on the basis of best lipase production. The maximum enzyme activity (34.93 U/ml) was shown by B. subtilis using SSF w...
Haniya Mazhar1, Naaz Abbas2, Tehseen Zamir3, Zahid Hussain1, Syed Shahid Ali4*
...ization. Best medium for lipase production was olive oil and mustard oil cake and the best carbon sources were fructose and maltose whereas yeast extract was found to be the best nitrogen source, showing 55U/mL enzyme activity. The best inoculum size for the growth of this strain was 4-5%. Optimum pH was 8 and best temperature was 40oC for the reported strain. Addition of divalent Mg2+ and Ca2+ ions resulted in 2-fold increase ...
Ghalia Nawal1, Muhammad Irfan2*, Saira Ashfaq3, Hafiz Abdullah Shakir1
...ganisms. Among microbial lipases, the bacterial lipases holds very prominent stance. The specificity of lipases is far-fetched for their point selection and kinds of reactions they undergo on their discernible substrates. Most suitable production plan involves Sub-merged liquid fermentation in fed batch manner using agricultural wastes. Bacterial lipase...
Yasir Ali1,2*, Bashir Ahmad1, Naqeebullah Jogezai3 and Adil Hussain1
...ease and alkaline active lipase producing psychrotrophic bacteria from water and soil samples collected from different glaciers of Karakorum Range of mountains, Pakistan. Serial dilution and plating approaches were exploited for bacterial isolation and the isolates were qualitatively screened for proteolytic and lipolytic activity with skim milk and Tributyrin agar (TBA plate) assay method. From the collected samples, a total of 20 bacterial isolates (PAK-01 t...
Parsa Riaz and Muhammad Naeem*
Digestive Enzymes Activity with Gut Morphometric Parameter of Carnivorous Fish Wallago attu (Siluridae, Siluriformes)
...gestive enzymes, such as lipases, proteases and amylases in the digestive tract of a wild, freshwater, carnivorous Wallago attu. Wallago attu is a fast growing catfish that belongs to family siluridae under the order siluriformes. It has good market demand as a food fish having high nutritional value, and high protein content in its flesh, Carnivorous in nature. Descriptive data of the studied traits included total body weight, total body length, gut weight, g...
Jen-Hong Chu1, Ya-Wen Lan2, Shyn-Shin Sheen3 and Angela Chien3*
Ramazan Erdogan1*, Mikail Tel2, Vedat Cinar2 and Ragip Pala2
...G), adipose triglyceride lipase (ATGL), glucose transporter-4 (GLUT-4) and insulin receptor substrate-1 (IRS-1) in adipose tissue of rats fed on zinc picolinate supplement diet. A total of 42 male 8-week-old Wistar albino rats were randomly divided into 6 groups, each of seven; Control (C), Zinc picolinate (ZP), Chronic exercise (CE), Chronic exercise+Zin picolinate (CE+ZP), Acute exercise (AE), Acute exercise+Zinc picolinate (AE+ZP). Rats were subjected to a ...
Zhengyi Fu1,2,3,4, Rui Yang2,3,4, Zhenhua Ma2,3,4*, Mingyang Han2,3,4 and Yifu Wang2,3,4
...he other two groups. The lipase activity of HL and HC was higher than that of control group in both stomach and foregut. This study indicates that increasing the proportion of lipid in the dietary improves growth performance and digestion performance of barramundi, while higher proportions of carbohydrate in the diet don’t affect their growth performance. Considering the cost of production, carbohydrate could be a potential energy supplementing source.
Nagarathinam Arunkumar*, Jainullabudeen Gulsar Banu, Nagarajan Gopalakrishnan and Arkalgud Hiriyannaiah Prakash
...numerous applications of lipases in chemical, food, pharmaceutical, biotechnological, detergents and surfactants, and agricultural industries and in waste treatment plants proved their importance. In this study, 23 wax-degrading bacterial strains isolated from four mealybug species infesting cotton were screened for lipase production. On the basis of the 16S rRNA gene sequences, lipase-pos...
Johar Hussian1, Faiz Muhammad2, Shafqat Fatimah Rehmani3, Aqeel Ahmad4, Nazir Ahmad Lone4, Moomal Bughio5, Syed Khurram Freed1 and Shakeel Ahmed Khan4*
...neuraminidase and phospholipase C. The prepared antigen were stored at -86oC and used for HA test. All antigen prepared by different enzyme were found to give significant HA titer up to 7 log2. During stability test antigen prepared by phospholipase C were found most stable up to six month by giving constant 7 log2 HA titer, while neuraminidase induced antigen were stable up to five months (7 log2). Trypsin treated antigen w...
Hung Van Mai1, Tuan Duc Nguyen2, Nang Thu Tran Thi3 and Hung Phuc Nguyen4*
...acid (BA) concentration, lipase activity, and lipid apparent digestibility coefficient (ADC) in pompano. The FSBM1 was produced by fermentation of defatted soybean meal (SBM) with Bacillus subtilis B3. The FSBM2 and FSBM3 were commercially available products, which were produced by fermentation of SBM with Lactobacillus spp. Five diets were formulated and denoted as FMD (fish meal-based diet), SBMD (SBM-based diet), FSBM1D (FSBM1-based diet), FSBM2D (FSBM2-bas...
Heba El-Zahar*, Zeinab Abd El-Rahman, Abbas El-Naggar
...rogen, blood creatinine, lipase and alkaline phosphatase (p<0.01) in IBD dogs. The mean values of IBD-related biomarkers (CRP, haptoglobin, and fecal calprotectin concentrations) increased significantly (p<0.05 or p<0.01) compared to controls. CRP, haptoglobin concentrations, and fecal calprotectin were found to have a strong positive correlation. Furthermore, blood enzymes, glucose and electrolytes were significantly correlated with CRP as an IBD-rel...
Laiba Shafique1*, Mahroze Fatima2, Syed Zakir Hussain Shah3, Muhammad Afzal4, Huma Naz5, Saif ur Rehman1 and Qingyou Liu1*
...ontrol diet. Activity of lipase enzyme was reduced by formic acid supplementation as well as vitamin D3. The observed formic acid and vitamin D3 (interactions) were synergistically bone mineralization of phosphorus, sodium, copper and also showed positive result for the activity of amylase, protease and lipase. Finally, formic acid and vitamin D3improves bone mineralization and digestive enzymes activity in the C. idella.
Xing Lu1, Mahmoud A.O. Dawood2, Fan Wu1, Hua Wen1, Wei Liu1, Juan Tian1, Ming Jiang1, Li-Juan Yu1, Xiang Li3, Ning Xu3 and Hong-Wei Liang1*
...1 (sod1) and lipoprotein lipase (lpl) genes in the liver of Chinese soft-shelled turtle (P < 0.05). All the above results indicated that appropriate dietary L-carnitine supplementation could improve growth performance, increase muscle protein content, reduce serum TG concentration, and induce the antioxidant- and lipometabolism-associated genes expression in Chinese soft-shelled turtle....
Ali Ahmad1*, Zubair Aslam1, Korkmaz Bellitürk2, Ehsan Ullah1, Ali Raza1 and Muhammad Asif3
...s (cellulose, proteases, lipase, amylases, chitinase), which degrade organic substrates even after they have been secreted. Earthworms change the chemical and physical characteristics of organic substrates, over time reducing the C:N ratio, increasing the surface area exposed to bacteria, and improving the microbial degradability of organic materials. This study aims to demonstrate how organic amendments, rather than chemical fertilizers, might improve soil he...
Müge Aliye Hekimoglu and Sırma Sönmez
...stive enzymes (protease, lipase, amylase), weight and length, body colour and survival rate. The image analysis method was used to determine skin colour. The chemical analysis method was used to determine the activity of enzymes. The results showed that supplementation of 2000 mg/kg of dietary significantly increased weight, length and specific growth rate (P < 0.05). While no change in colour was observed in the control group at the end of the experiment, ...
Jie Chen, Qinghua Qiu, Yanjiao Li, Xianghui Zhao, Lanjiao Xu, Xiaowen Xiong, Qingqi Wen, Mingren Qu and Kehui Ouyang*
... i.e., hormone-sensitive lipase (HSL) expression in visceral pre-adipocytes were higher than that in subcutaneous pre-adipocytes, but adipogenesis transcriptional factors such as sterol regulatory element-binding protein 1c (SREBP-1c) and peroxisome proliferator-activated receptor-gamma (PPAR-γ) were lower than that in subcutaneous pre-adipocytes. Moreover, the LGM group increased the expressions of LC-3, reduced the p62 in both subcutaneous and visceral...
SYED ALI RAZA1, MUHAMMAD WASEEM MUMTAZ2*, AYOUB RASHID CH1, ABDUL WAHEED1, JAMES WILLIAM3 & MUHAMMAD ARSHAD1
...tioxidant and pancreatic lipase inhibitory properties of different hydroethanolic leaf extracts of Conocarpus lancifolius. Freeze drying assisted ultra-sonication was adopted for extract preparation. The results showed that highest total antioxidant power of 291 ± 0.50 mg ASE/g DE was exhibited by 60% ethanolic extract which was slightly lower than antioxidant power of BHA (309.16 ± 3.90 mg ASE/g DE). The β-carotene bleaching inhibiti...
Saddam Hussein Mahmoud* and Shaimaa Nabhan Yassein
...edium. Hemolysis, phospholipase activities, and biofilm formation were among the virulence tested factors. Hemolysis and phospholipase activities were detected in 76.2% and 83.33% of C. albicans isolates, respectively, while biofilm formation was detected in 100% of C. albicans isolates. The diagnosis was confirmed using 25 of the most virulent C. albicans isolates, by polymers chains reaction (PCR) to detect the KER1 gene r...
Muhammad Shoaib1, Muhammad Mahboob Ali Hamid1, Shafaq Amir2, Shaukat Ali Bhatti1*, Hafiz Hassan Iqbal1, Najam-us-Sahar1 and Mubsher Hussain1,3
...etermine the efficacy of lipase and emulsifier + lipase in broilers reared on energy diluted diet. Five hundred and twenty birds were divided into 8 treatments groups with 5 replicates of 13 birds in each. Total 8 diets viz., PC (Positive control), NC1 (Negative control 1; 75 Kcal/kg reduced energy), NC1L (NC1 + lipase at 0.015%), NC1LB (NC1 + lipase at ...
Jacob Situmeang1, Lovita Adriani1*, Deny Saefulhadjar1, Safri Ishmayana2
... yogurt has protease and lipase enzyme activity, which can increase digestive tract enzymes in laying hens. In this study, it is hoped that probiotics can increase protein levels and reduce lipid and cholesterol levels in chicken egg yolks. This study aims to determine the activity of the protease and lipase enzymes from yogurt and the effect and level of use of probiotic powder, which can increase protein and reduce lipid a...
Ahsan Abbas Abro1, Imrana Khushk1, Abdul Nabi Jatt2, Chaudhary Haider Ali3, Abdul Sattar Qureshi1*, Farah Naz Talpur4, Safia Bano1
...tify;">Aspergillus niger lipase was employed in transesterification of used cooking oil into biodiesel. Fungus cultivated in molasses mineral medium with tryptone as the nitrogen source for 5 days at 37 °C and 6.0 pH, strain produced a maximum lipase concentration of 29.39 (U/mL). Effect of metal ions, pH, pH stability, temperature, and thermostability on lipase activity were examined....
Maysoon S. Abbas, Shaimaa N. Yassein
...s of V. cholerae. Phospholipase activity showed a positive result in 6 out of 9 isolates (66.66%), proteinase activity in 7 out of 9 isolates (77.77%), while hemolytic activity and biofilm formation were positive in all V. cholerae isolates, with a 100% rate. For the first time in Iraq, isolation of Vibrio spp. has been reported in birds and owners. This study concludes that the high percentage of Vibrio spp. in birds is linked to the significance of zoonotic ...
Ria Dewi Andriani1, Lilik Eka Radiati1, Teti Estiasih2*
...oldband snapper visceral lipase. The glycerides composition of HGSVO was monoglyceride, diglyceride, triglyceride, glycerol, and free fatty acids, and contained omega-3 fatty acids comprising of docosahexaenoic acid (DHA) of 17.62% and eicosapentaenoic acid (EPA) of 3.60%. HGSVO was formulated in dairy creamer at concentrations of 0.2%, 0.4%, and 0.6% (w/v). The results showed that the differences formulated of HGSVO in dairy creamer had a significant effect (...
Ayub Khawar1, Noor Khan1*, Khalid Javed Iqbal2, Mahroze Fatima1, Fayyaz Rasool3, Khalid Mahmood Anjum4, Hamda Azmat1, Shahid Sherzada1, Anjum Khalique5, Sadia Nazir1, Sheeza Bano1, Sakhawat Ali6 and Muhammad Asghar1
Baraa Qasim Mohammed1, Asmaa Hamoody Abdullah1, Ahmed Raheem Rayshan2*
...ctor hemolysin, a phospholipase enzyme. The 16S rRNA gene was first identified as a virulence gene by polymerase chain reaction study, followed by the identification of the exotoxin T, outer membrane protein I, and phenzaine+ M genes. Three (27.27%) of the isolates were P. aeruginosa carrying exoT genes, four (36.36%) P. aeruginosa isolated carried oprl genes, and three (27.27%) isolates of P. aeruginosa carrying phz+M genes. By using traditional PCR, oprl had...
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