Faiza Ambreen*1 and Muhammad Javed2
...fos) in peripheral blood erythrocytes of freshwater fish, Oreochromis niloticus by using Comet assay. The 96-h LC50 value of pesticide mixture was estimated for 180-old fingerlings of O. niloticusin a static system and then four sub-lethal concentrations viz. 1/3rd of LC50, 1/4th of LC50, 1/5th of LC50 and 1/6th of LC50 were calculated and fi...
S. Jalbani1, N. T. Narejo2,*, P. Khan2 and Y.M. Jalbani3
...ifferences between total erythrocytes count, hemoglobin concentration and erythrocyte sedimentation rate of the two sexes. Male fish had total erythrocytes 5.05 ± 0.5 × 106 and hemoglobin content 11.5 ± 1.5 g/in ml compared to 4.04 ± 0.5 × 106 and 7.3 ± 1.3 g/in ml in the female. The female fish had significantly higher values of ESR in comparison of male. It may ...
Sheng Niu1, Ali-Raza Jahejo1, Fa-jie Jia1, Xin Li1, Guan-bao Ning1, Ding Zhang1, Hai-li Ma1, Wei-fang Hao2, Wen-wei Gao1, Yu-jun Zhao1, Shi-min Gao1, Jian-hui Li1, Gui-lan Li1, Fang Yan1, Rong-kun Gao1, Huan-chun Chen1,3 and Wen-xia Tian1,*
... been shown that chicken erythrocytes constitutively express toll-like receptors (TLRs), cytokines, and avian β-defensins (AvBDs). Host defense peptides (HDPs) are important effector molecules in innate immunity. In this study, thiram has been used to induce tibial dyschondroplasia (TD) and the chickens were injected with different doses of recombinant glutathione-S-transferase A3 (rGSTA3) proteins. We explored the responses of HDPs in
Tian-Yi Zhao1, Zi-Qing Liu2, Bo Yang1, Qiao Gao1, Hong-Yu Zhang1, Pei-Yu He1, Ming-Hua Duan1* and Yu-Li Yan1*
...ncrease in the number of erythrocytes and a significant increase in hemoglobin content (P < 0.05). Compared with the control group, 5-Fu group showed a significant decreased in the number of CD71/Ter119-labeled erythrocytes (P < 0.001) and CD4/CD8-labeled T cells (P < 0.001) in peripheral blood and the spleen of mice, which was restored significantly in the number of CD71/Ter119-labeled
Mumtaz Ali Khan1, Sher Bahadar Khan2, Shakoor Ahmad2, Irshad Ahmad3, Kashif Prince1*, Ghazunfar Rashid1, Mahboob Ali1, Imdad Ullah Khan4Asad Ullah5, Naimat Ullah4, Muhammad Shoaib2 and Said Sajjad Ali Shah2
...(P<0.05) decreases in erythrocytes counts (RBC) and hemoglobin levels while the mean WBC, platelets, packet cell volume, serum creatinine, bilirubin, liver enzymes, total, glucose and urea in blood significantly (P<0.05) increased. Fluctuations in mean erythrocyte counts (RBC), hemoglobin, PCV and total bilirubin were beyond the limits while others were within normal ranges in sheep infected with Clostridium perfringens type A. The total bilirubin...

Turgay Şişman1,*, Muhammet Çağrı Keskin1, Hatice Dane1, Şeymanur Adil1, Fatime Geyikoğlu1, Suat Çolak2 and Esra Canpolat1

...ormalities of peripheral erythrocytes and lung, liver, and kidney histopathologies were evaluated in marsh frog (Pelophylax ridibundus) in agricultural areas. The most prominent histopathological alterations observed in lung tissue were noted as epithelial hyperplasia, thickness of alveolar septa, dilatations and congestions of blood capillaries, and melanomacrophage accumulation. An increase in the number and widening of melanomacrophage centers were a...
Muhammad Farhan Qadir1, Xin-Yu Han1, Meng-li Qiao1, Ying Wang1, Ding Zhang1, Yu-hai Bi1,2, Ali Raza Jahejo1,Qian-qian Cheng1 and Wen-xia Tian1,*
... small;">Chicken’s erythrocytes are most abundant cells circulating in the body which participate in inflammation and immune responses. However, expression levels of prostaglandins-related genes in H9N2 infected chicken’s erythrocytes have not been studied yet. So, the present study was designed to investigate the PG-related genes in erythrocytes of H9N2 infected chickens. In c...

Saddar Faheem1,2, Hameeda Kalhoro1, Naeem Tariq Narejo1*, Muhammad Hanif Chandio3, Memon Samina4, Shahnaz Rashid5 and Ghulam Abbas5*

...ely. Results showed that erythrocytes (2.09 × 106 to 2.26 × 106), leucocytes (164.90 × 103 to145.55 × 103) and haemoglobin (10.34 g/100 ml to 7.06 g/100 ml) concentration were significantly higher in male fish during summer and winter than that of female. In female fish, erythrocyte sedimentation rate (ESR) in summer and winter season was found higher (7.3 mm/h to 2.56 mm/h). Thrombocytes, neutrophils, lymphocytes, eosinophil and monocy...
Guan-bao Ning1, Sheng Niu1, Yue-jian Li1,2, Xiao-xiao Lu1,3, Shi-xiong Yang1,  Ali-Raza Jahejo1, Ding Zhang1, Wei-fang Hao4, Wen-wei Gao1, Yu-jun Zhao1, Jian-hui Li1, Fang Yan1, Rong-kun Gao1, Yu-hai Bi1,5, Wen-xia Tian1* and Ling-xia Han6*
...MD) in chickens. Chicken erythrocytes are nucleated cells of myeloid origin which contribute to gases exchange, transportation and immunity. The objective of this study was to reveal the immune responses of MDV-infected erythrocytes of chicken by transcriptome analysis. Twenty specific pathogen-free (SPF) chickens were divided into two groups as control and treatment. The chickens of treatment group were inoculated with MDV,...
Nianhong Huang, Yan Wu, Yuanyuan Li* and Jinhong Zhao*
...ized and non-parasitized erythrocytes showed that most analyzed features were significantly different for both linear and area dimensions. The sequences of 18S rRNA gene of Hepatozoon sp. were amplified, cloned and demonstrated the homology and the variation. Phylogenetic analysis based on the 18S rRNA gene analyzed in the present study together with other published Hepatozoon species showed that there is no significant difference in the geograph...

Sajid Abdullah1*, Huma Naz2*, Zunera Abbas1, Uzma Nazir1, Mehrunisa Basharat1, Tanveer Ahmed3, Adnan Ahmad Qazi2

...ormalities in peripheral erythrocytes of Wallago attu were assessed by micronucleus assay. Fish were exposed to different concentrations (1/3rd, 1/4th, 1/5thand 1/7th of LC50) of Pb, Cr and Cd, separately, for three weeks. Blood sample of fish was collected to see the micronuclei (MN) and deshape nuclei (DN) in peripheral erythrocytes of fish after one-week interval. Fish W. attu showed significant induction of MN and DN in ...
Rizwana Sultan1, Asim Aslam1, Muhammad Yasin Tipu1, Habib ur Rehman2, Saba Usman1, Ahsan Anjum1,*, Muhammad Saeed Imran1, Muhammad Usman3 and Muhammad Zahid Iqbal3
...ated a decrease in total erythrocytes, packed cell volume, haemoglobin concentration, and red blood cell indices. Differential leukocyte analysis revealed leukocytosis, heterophilia, eosinophilia, monocytosis, and lymphocytosis. Serum biochemistry showed marked elevation in aspartate transaminase, alkaline phosphatase and creatinine and a significant decline in alanine transaminase, total protein, total albumin, globulin, triglycerides and cholesterol values. ...
Muhammad Farhan Qadir1, Xin-Yu Han1, Meng-li Qiao1, Qian-qian Cheng1
Raza Ali Mangi1, Ali Raza Jahejo1, Afrasyab Khan1, Yu-hai Bi1,2 and Wen-xia Tian1*
...to human health. Chicken erythrocytes are known as most abundant circulating cells in the body which participate in inflammation and immune responses. Influenza viruses interact with the host cells at cell surface, leading to cell death in human and animals through apoptosis. Current study was designed to investigate the expression levels of apoptosis-related genes in the H9N2 infected chicken erythrocytes. qRT-PCR and Weste...

Mariam Arain1, Asghar Ali Kamboh1* and Muhammad Javed Arshed2

... hemoglobin, hematocrit, erythrocytes, mean cell hemoglobin, mean corpuscular volume, mean corpuscular hemoglobin concentration, leukocytes, neutrophils, lymphocytes and monocytes count were significantly higher (p<0.05) in the Se treated group compared to control. It was concluded that Se supplemented goats have better gut microflora composition and immune response compared to goats fed on basal diet without Se supplementation.

...

Ahlam G. Khalifa1*, Walaa A. Moselhy1, Hanaa M. Mohammed2 and Khaled Abdou1 

...e ratio of polychromatic erythrocytes to normochromatic erythrocytes proved that deltamethrin had a stronger cytotoxic effect on the bone marrow cells than the CS/DM Nps and S/DM Nps groups. It was concluded that these innovative nanoformulations of DM were less toxic, which would lower health risks and boost application safety.

Keywords | Cytotoxic, Chromosome aberrations, Loaded, Nanopesticides, Safety 

...

Febriza Dwiranti1, Nur Fadhilah2, Ursula Paulawati Maker2, Priyo Sambodo3* 

... males and the number of erythrocytes and leukocytes (the highest number of neutrophils), respectively, were higher than the results in the foregoing study. Conclusion: There are different body hair patterns in male and female S. papuensis. Additionally, obvious differences in certain blood values of cuscus are generally assumed to be common phenomena in vertebrates, especially mammals.

Keywords | Blood value, cuscus, endemic, morphology, Papua 

Tertia Delia Nova1*, Yulia Yelita2, Rizky Machicula1

...erved were the number of erythrocytes, hemoglobin levels, hematocrit percentage, the number of lymphocytes, monocytes, heterophils, eosinophils, and basophils. Three treatments of 5%, 10%, and 15% levels of kiambang flour in feed were ready. The variables measured were also ration consumption, weight gain and conversion as well as carcass, and abdominal fat during growth. The experimental results showed that the average feed consumption ranged from 4034.89 to ...
Irfan Shahzad Sheikh1, Syed Haseeb Shah1, Niamatullah Kakar2*, Mohammad Masood Tariq1, Muhammad Essa Kakar3 and Muhammad Zahid Mustafa1
...y on hemoglobin (Hb) and erythrocytes (RBCs) in all treatment groups treated till day 42 compared with the control group. Remarkably, the AGPs showed a positive impact on the liver enzymes such as alanine aminotransferase (ALT) and aspartate aminoransferase (AST) on day 42. A non-significant effect was seen on hematological and biochemical profile of liver and kidney of broiler chicken on days 14th and 28th. In conclusion, the study showed significant synergis...

Sehrish Ashraaf1, Hafiz Muhammad Tahir1*, Chand Raza1, Ayesha Muzamil1, Farman Ahmad Chaudhary2, Muhammad Summer1 and Aamir Ali1

...bserve the mean count of erythrocytes, hemoglobin, platelets, white blood cells (neutrophils, monocytes, eosinophils and basophils) in control and experimental groups. Based on the preliminary results a mixture of best concentration of sericin and curcumin was successfully achieved and was used in further studies. In the group that was given carrageenan alone, the number of erythrocytes, hemoglobin and platelets were decreas...

Sehrish Ashraaf1, Hafiz Muhammad Tahir1*, Chand Raza1, Ayesha Muzamil1, Farman Ahmad Chaudhary2, Muhammad Summer1 and Aamir Ali1

...bserve the mean count of erythrocytes, hemoglobin, platelets, white blood cells (neutrophils, monocytes, eosinophils and basophils) in control and experimental groups. Based on the preliminary results a mixture of best concentration of sericin and curcumin was successfully achieved and was used in further studies. In the group that was given carrageenan alone, the number of erythrocytes, hemoglobin and platelets were decreas...

Aamir Mushtaq1*, Fatima Habib2, Umar Farooq Gohar3, Abdul Malik4 and Mobasher Ahmad2

... not affect the level of erythrocytes, leukocytes, thrombocytes, hemoglobin, hematocrit, creatinine and liver enzymes (SGOT, SGPT and TB) in mice. However, it significantly (P ≤ 0.05) increased the level of ALP in mice but on the other hand it lowered the blood glucose and cholesterol levels significantly along with increase in body weight of mice. It is concluded that P. anisum methanolic extract has a broad therapeutic index and does not produce considera...

Sukandi Sukandi1,4, Djoni Prawira Rahardja2*, Herry Sonjaya2, Hasbi Hasbi2, Sudirman Baco2, Sri Gustina3, Kirana Dara Dinanti Adiputra1

...d hematological profile (erythrocytes, hemoglobin, hematocrit, and leukocytes). Body physiological and hematological data were analyzed using factorial analysis to compare Bali cattle (horned and polled) and the time factor of measurement (morning and afternoon), followed by Duncan’s test. The results showed that horned and polled Bali cattle were significantly (P<0.05) different in rectal temperature, erythrocytic counts, hemoglobin levels, and hemat...

Ertika Fitri Lisnanti1,4, Widya Paramita Lokapirnasari2*, Eka Pramyrtha Hestianah3, Mohammad Anam Al Arif2, Zein Ahmad Baihaqi4,5 

...he number of leukocytes, erythrocytes, hemoglobin levels, hematocrit, platelets, lymphocytes, monocytes, granulocytes, types of leukocytes and blood glucose levels in broilers. The addition of different of Marsh fleabane leaves water extract in the feed had a significant effect on the levels of Hb, leukocytes and erythrocytes were within the normal range while all blood glucose was in a normal state apart from P4 (decrease),...

Afrasyab Khan1,3, Ali Raza Jahejo1,3, Meng-li Qiao1,3, Xin-yu Han1,3, Raza Ali Mangi1,3, Ding Zhang1,3, Yu-hai Bi2, George F Gao1,3* and Wen-xia Tian1,3*

...es expression in chicken erythrocytes infected with Mycoplasma synoviae (M. synoviae). Therefore, the aim of the current in-vitro study was to determine the interaction between chicken erythrocytes and M. synoviae using Transmission electron microscope (TEM) and further to investigate the mRNA gene expression of MyD88 (Myeloid differentiation primary response 88), CCL5 (C-C Motif Chemokine Ligand 5), MDA5 (melanoma different...

Elly Roza*, Salam N. Aritonang, Yulia Yellita, Hilda Susanty, Rizqan

...udy were the hematology (erythrocytes, leukocytes, hemoglobin, and hematocrit) of Murrah buffalo. The results obtained in sequential studies are as follows: Erythrocytes (3.94 – 5.30 x106/ µL), Leukocytes (6.6 – 9.8 x103/µL), Hemoglobin (8.9 – 9.8 g/dL) Hematocrit (21.2 – 24.2%) and milk production (3.75 – 5.95 kg/head/day). From the results of the study, it was found that feeding 50...
Raza Ali Mangi1,2, Ali Raza Jahejo1,2, Afrasyab Khan1,2, Meng-li Qiao1,2, Muhammad Farhan Qadir1,2, Mazhar Hussain Mangi3,  Shi-xiong Yang1,2, Xin-yu Han1,2, Sheng Niu1,2, Ding Zhang1,2, Ying Wang1,2 and Wen-xia Tian1,2*
...red by TD index. Chicken erythrocytes constitutively expressed transcripts for HDR and PRRs genes. Moreover, the quantitative real time PCR (qRT-PCR) results showed that HDRs and PPRs expressions were suppressed by thiram, which found upregulated in rGSTA3 protein supplementary group. These findings demonstrated that mRNA expressions of HDRs and PPRs are highly related to thiram. Moreover, rGSTA3 protein increased the immune response of HDRs and PPRs. Conclusi...

Xin-yu Han1,2, Afrasyab Khan1,2, Ali Raza Jahejo1,2, Qian-qing Cheng1,2, Meng-li Qiao1,2, Raza Ali Mangi1,2, Muhammad Farhan Qadir1,2, Ding Zhang1,2, Ying Wang1,2, Yu-hai Bi1,3, Rui-wen Fan1,2 and Wen-xia Tian1,2

...le TLRs genes in chicken erythrocytes, studies have been lacking. This study investigated the expression profile of TLRs pathway immune gene in chicken erythrocytes in response to Mycoplasma synoviae. The purpose of the current in-vitro study was to determine the chicken erythrocytes interaction with M. synoviae using a transmission electron microscope (TEM). The mRNA gene expression of TL...

Pakistan Journal of Zoology

February

Pakistan J. Zool., Vol. 56, Iss. 1, pp. 01-501

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