Articles
183 open-access articles · page 2 of 10
Research ArticleBreeding Biology and Egg Parameters of Red-Wattled Lapwing (Vanellus indicus) in Agri-Fields of Gomal Landscape, District Tank, Khyber Pakhtunkhwa, Pakistan
Shahid Amin, Aziz Ur Rehman, Umer Farooq, Muhammad Fawad Fareed, Ashfaq, Imran khan et al.
The study investigated various features of the breeding-Biology of Red-Wattled Lapwing (Vanellus indicus) and characteristics of its eggs in agricultural fields of Gomal, district Tank. The male bird chose a location for the nesting season and the both birds (…
9 Apr 2025DOI 10.17582/journal.Biologia/2025/71.1.1.5
Research ArticleGreen-Synthesis-Zinc-Oxide-Nanoparticles-Bacillus
Mehwish Iqtedar, Mahnoor Siddique, Asma Shahzad, Afshan Kaleem, Roheena Abdullah
Zinc oxide nanoparticles are widely and safely used due to their characteristic nature. The current study is focused at microbial synthesis of Zinc oxide nanoparticles (ZnO NPs) and their applications in biomedical and environmental areas. Intracellular ZnO NP…
21 Mar 2025DOI 10.17582/journal.Biologia/2024/70.2.66.74
Research ArticleLegal and Illegal Trade of Wildlife and Domesticated Birds in District Tank, Khyber Pakhtunkhwa: A Case Study of Two Local Markets (Mandies)
Shahid Amin, Muhammad Fawad Fareed, Inayat Ullah, Aziz Ur Rehman, Umer Farooq, Danial Bakhsh Saigra et al.
The commercial use of wild plants and animals is commonly referred to by means of wildlife-trade. The data was collected from two (02) unlike localities of the district Tank. The data was proofed on a questionnaire beside during animal classification, local an…
9 Mar 2025DOI 10.17582/journal.Biologia/2024/70.2.61.65
ReviewBacterial Diversity Associated with Dung Beetles: Ecological Perspectives
Sheeza Sakhawat, Aniza Iftikhar, Uswa Zeb, Aqsa Noreen, Suleman Hussain Shah, Mubashar Hussain
This study reviews the bacterial diversity associated with the gut microbiota of dung beetles, focusing on the microbial ecology, taxonomy, and environmental factors influencing the composition and functionality of these bacterial populations. Dung beetles are…
8 Feb 2025DOI 10.17582/journal.Biologia/2024/70.2.54.60
Research ArticleDiversity and Distributional Patterns of Grasshoppers in Croplands of District Gujrat, Punjab, Pakistan
Mubashar Hussain, Syeda Nafeesa Kazam, Aqsa Noreen, Suleman Hussain Shah, Uswa Zeb, Aniza Iftikhar
Grasshoppers are major herbivores that occupy agricultural landscapes across the globe due to their ecological, behavioural, and taxonomic diversification. The periodic assessment of the population of grasshoppers in field crops is crucial in devising and impl…
18 Jan 2025DOI 10.17582/journal.Biologia/2024/70.2.38.46
Research ArticleAnalysis of Clinical and Hematological Findings in Malaria Infected Patients in Hyderabad, Sindh
Zarina Chang, Nadir Ali Birmani, Adil Hassan Chang, Laraib Jamali, Noreena Chang, Fouzia Chang
To investigate the effects of Plasmodium species on hematological parameters in a descriptive case study was carried out on malaria positive patients. The objective of the study is to determine how malaria disturbs complete blood cell parameters. If it is left…
18 Jan 2025DOI 10.17582/journal.Biologia/2024/70.2.47.53- Research Article
Effect of Different Lysine Regimen on Performance Characteristics and Economic Efficiency of First Progeny of Aseel Parents Flock
Tahira Batool, Salman Farooq, Nabila Roohi, Abdul Ghayas
This experiment was designed to evaluate the effects of various lysine regimens on the growth performance of local Aseel chicken progeny. In total, 240 day-old chicks constituting 60 of each Lakha, Peshawari, Mianwali and Mushki varieties, selected and placed…
5 Jan 2025DOI 10.17582/journal.Biologia/2024/70.1.30.37 
Research ArticleErysiphe diffusa, a New Record for Powdery Mildew Fungi from Pakistan Breeding
Afshan Akbar, Saliha Afzal, Najam Ul Sehar Afshan, Muhammad Fiaz, Abdul Nasir Khalid
During mycological field surveys in 2021, infected leaves of Lespedeza tomentosa were gathered from District Swat and Battagram, Khyber Pakhtunkhwa, Pakistan. The infection symptoms seemed to be white powder on foliage with numerous tiny blackened to brownish…
5 Jan 2025DOI 10.17582/journal.Biologia/2024/70.1.16.20
Research ArticleProcess Optimization for the Production of Alpha Amylase by Aspergillus niger Kbt-3 Using Submerged Fermentation
Roheena Abdullah, Kinza Nisar, Afshan Kaleem, Mehwish Iqtedar
The use of alpha amylase as a substitute for chemical processes has gained significant momentum in the field of biotechnology. As a result, there is a growing need to explore untapped reserves of fungi with high potential for efficient alpha amylase production…
5 Jan 2025DOI 10.17582/journal.Biologia/2024/70.1.21.29
Research ArticleRelationship of Anosmia with Nasopharyngeal Swab-Based Sample Collection for Covid-19
Muneer Ahmed Solangi, Mahvish Jabeen Channa
COVID-19 caused a significant number of infections and deaths since first appearance in December 2019 in Wuhan, China. Anosmia and hyposmia had been viewed as the first signs of COVID-19. However, these could also be triggered by preceding factors in the popul…
4 Jan 2025DOI 10.17582/journal.Biologia/2024/70.1.8.15
Research ArticleIn Silico and In Vitro Analysis of Red Kidney Beans (Phaseolus vulgaris) and Black-Eyed Beans (Vigna unguiculata) and Their Phytochemical Analysis
Afshan Kaleem, Iqra Noor, Roheena Abdullah, Mehwish Iqtedar
Beans and legumes are an excellent source of protein, minerals, dietary fiber, carbohydrates and bioactive compounds. They serve as medicinal plants and are used as common food in developing countries. The objective of the current study was to analyze the vari…
4 Jan 2025DOI 10.17582/journal.Biologia/2024/70.1.1.7- Research Article
Dietary inclusion of phytogenic feed additives (PFA) as alternative to in feed antibiotics modulating gut microbial dynamics in broiler chicken
ZUBIA RASHID, ZULFIQAR ALI MIRANI, SITWAT ZEHRA, SYED MUDDASSAR HUSSAIN GILANI, ARIF ALI CHISHTI, ABID AZHAR et al.
Food safety and quality have been a significant and crucial predicament recently. In poultry, development of novel antimicrobial strategies for drug-resistant pathogens is desired. Here, Phytogenic Feed Additives (PFA) were administered in chicken diet to deci…
27 Feb 2024 - Research Article
Heavy metals (Cd, Pb & Zn) accumulation in Cauliflower (Brassica oleracea. Var. Botyris) and associated health risks assessment in three Districts of Punjab, Pakistan
ZEESHAN REHMAN, RANA ABRAR HUSSAIN, SHAISTA JABEEN, SAKHAWAT ALI, ZAHAR NOREEN, IRUM MUKHTAR
Continuous discharge of untreated urban and industrial waste water is a source of heavy metals in soil is a major threat to ecological system and human health. Present study was carried out to evaluate human health risks in association with food chain contamin…
27 Feb 2024 - Research Article
Antimicrobial activities of Methanolic and Aqueous extracts of Nigella Sativa and Ficus Carica
SHAHZADA NADEEM ABBAS, KIRAN JAMSHED, SARAH BUSHRA NASIR, SAMAN SHAHID, NASIR MAHMOOD
New antimicrobials agents are needed to combat drugs resistance in microorganisms. The aqueous and methanolic extracts of Nigella sativa and Ficus carica seeds were used against bacteria (Staphylococcus aureus and Escherichia coli) and Fungi (Candida albicans,…
27 Feb 2024 - Research Article
Monitoring mangroves plantation sites through integration of repeat terrestrial photographs and spaceborne imagery
UROOJ SAEED, SAJID RASHID AHMAD, HAMMAD GILANI, RAB NAWAZ, NAEEM SHAHZAD, IRFAN ASHRAF et al.
This study shows the outcome of realistic steps towards the coastal habitat conservation through mangroves plantation in Keti Bunder (Indus delta), Thatta district, Sindh province, Pakistan. From the visual interpretation of repeat terrestrial photographs and…
27 Feb 2024 - Research Article
Assessment of Roadside Soil and Brassica Rapa Contamination by Heavy Metals in District Buner, Pakistan
MUJIBUR RAHMAN, MUHAMMAD AYAZ KHAN, WISAL SHAH, ALIA NAZ
This study was conducted in Buner district of Pakistan to investigate the presence of Cadmium (Cd) and Lead (Pb) in roadside soil and Brassica rapa (Shalgham). For that purpose, soil and B. rapa samples were collected from primary, secondary, tertiary roadside…
27 Feb 2024 - Research Article
Evaluation of antioxidative and antidiabetic potential of piper betle leaf in alloxan induced diabetic albino Rats
HAFIZ MUHAMMAD ARSALAN, SUMMARA TABASSUM, MUHAMMAD SHERAZ YASIN, FAROOQ SALEEM, ZEEMAL SEEMAB AMIN, MUNIB ASHFAQ et al.
Piper betle leaf is deep green heart shaped belongs to family Piperaceae. This plant is used for the treatment of different diseases like diabetes, cancer, inflammatory responses, oxidant injuries etc. in the present study ethanolic extract of Piper betle leaf…
27 Feb 2024 - Research Article
Sex determination through Barr bodies of neutrophils in Humans and Dogs
MUSHTAQ HUSSAIN LASHARI, RABIA HAMEED, UMER FAROOQ, MUSADIQ IDRIS, ZIA UR REHMAN
Blood samples of healthy adult humans (n = 40) and dogs (n = 30) grouped as males and females, were collected aseptically through appropriate measures. Thin blood smears were stained using Field stain ‘A’ and ‘B’. The PMNs were counted…
27 Feb 2024 - Research Article
Enhanced Sodium Chloride disturbs the Genetic stability in Ocimum tenuiflorum L.
MUBEEN RIAZ, KIRAN ZAHID, SUMAIRA ASLAM CHOHAN, MUHAMMAD ASHFAQ, MUHAMMAD ALI, RIFFAT SIDDIQUE et al.
The present investigation was carried out to evaluate the effects of NaCl stress on genetic stability of lines of Ocimum tenuiflorum L. using RAPD markers. For genetic characterization of controlled and NaCl stressed plants, the extracted DNAs were subjected t…
27 Feb 2024 - Research Article
Proteolytic activity analysis in germination defective spores of Bacillus
Uqba Mehmood
Proteolytic enzymes are characteristically produced by Bacillus species during sporulation and germination. The hydrolysis of spore proteins to free amino acids, is accomplished by proteases, in first 20 minutes of spore germination. The analysis of Spo mutant…
27 Feb 2024