Piper betle leaf is deep green heart shaped belongs to family Piperaceae. This plant is used for the treatment of different diseases like diabetes, cancer, inflammatory responses, oxidant injuries etc. in the present study ethanolic extract of Piper betle leaf was used (300 mg/kg). Diabetes was induced in male albino rats by using Alloxan (150 mg/kg). Glutathione (GSH), Catalase (CAT), Malondialdehyde (MDA), Nitric oxide (NO), micronutrients (Vitamin A, Vitamin C and Vitamin E), serum glucose level and Advance glycation end products (AGE’s) were estimated. The results showed that levels of the Malondialdehyde (0.087, 0.151 and 0.104 respectively), Advance glycation end products (0.026, 0.053, 0.025) and Nitric oxide (0.07, 0.438, 0.190) were found decreased while level of the GSH (0.184, 0.188, 0.207), CAT (0.087, 0.031, 0.091) and vitamin A (0.196, 0.136, 0.178), E (0.199, 0.140, 0.179) and C (0.163, 0.120, 0.164) were increased after administration of Piper betle leaf extract. Blood glucose level was also found to be decreased. It is concluded that Piper betle leaf can be used as antidiabetic drug because it is involved in lowering the blood glucose level and Piper betle leaf is a good source of anti-oxidants.