Regulation of Collagen Metabolism in Keratinocyte-Fibroblast Organotypic Co-culture by Pressure
Regulation of Collagen Metabolism in Keratinocyte-Fibroblast Organotypic Co-culture by Pressure
Yang Liu1,2,3, Baimei Liu1,2,3, Chenchen Li1,2,3 and Meiwen An1,2,3,*
A, SEM morphology of CG three-dimensional scaffolds; B, keratinocytes; C, Fusion of fibroblasts; D keratinocytes morphology on CG scaffolds, HE stains of group B (KM in KM&FM without pressure); E, fibroblasts morphology on CG scaffolds, HE stains of group F (FM in KM&FM without pressure).
A, the comparation expression of type I collagen mRNA in KM; B, type III collagen mRNA expression in KM; C, type I collagen mRNA expression in FM; D, type III collagen mRNA expression in FM; E, production of type I collagen; F, production of type III collagen: a to d shows significant difference of protein expression of KM (*P<0.05), and e and f shows significant difference of protein expression of FM (*P<0.05). n=3.
A, the comparation expression of IL-1α mRNA in KM; B, the comparation expression of IL-1α mRNA in FM; C, the production of IL-1α; D, the comparation expression of MMP-3 mRNA in KM; E, the comparation expression of MMP-3 mRNA in FM; F, the production of MMP-3: a to d shows significant difference of protein concentration of KM (*P<0.05), and e and f shows significant difference of protein concentration of FM (*P<0.05). n=3.
