Protective Effects of Tea Leaf Extract (Camellia sinensis) Against Cypermethrin-Induced Toxicity in Gill and Liver of Rainbow Trout (Oncorhynchus mykiss)
Protective Effects of Tea Leaf Extract (Camellia sinensis) Against Cypermethrin-Induced Toxicity in Gill and Liver of Rainbow Trout (Oncorhynchus mykiss)
Tayfun Karatas1*, Betul Apaydın Yıldırım2 and Serkan Yıldırım3
Effect of tea leaf extract on histological structure of fish liver induced with cypermethrin. A, normal histological structure of liver. B shows severe degeneration (thin arrow), severe steatosis (arrowhead), and severe necrosis (thick arrow) in hepatocytes, as well as severe inflammation (star) after treatment with cypermethrin. C shows effect of 50 mg of TLE on CMN exposure, modarate degeneration, modarate steatosis and mild necrosis in hepatocytes, mild hyperemia (empty star) and inflammation (star) is visible. D shows effect of 100 mg of TLE on CMN exposure, mild steatosis in hepatocytes (arrowhead) is visible. H and E, Bar: 50 µm.
Effect of tea leaf extract on histological structure of fish gill induced with Cypermethrin. A normal histological structure of gill. B shows severe adhesion (thick arrow), severe necrosis (thin arrow), severe inflammation (star), and severe degeneration (arrowhead) in gill lamellar epithelium after treatment with cypermethrin. C shows effect of 50 mg of TLE on CMN exposure, severe degeneration (arrowhead), mild necrosis (thin arrow), and moderate adhesion (thick arrow) in gill lamellar epithelium is visible. D shows effect of 50 mg of TLE on CMN exposure, moderate degeneration (arrowhead) and mild adhesion (thick arrow) in gill lamellar epithelium is visible, H and E, Bar: 50 µm.
Effect of tea leaf extract on 8-OHdG expression in liver hepatocytes. A shows negative 8-OHdG expression in hepatocytes. B shows severe 8-OHdG expression in hepatocytes (arrowhead) of fish after exposure to CMN. C shows moderate 8-OHdG expression in hepatocytes of 50 mg of TLE on CMN exposure. D shows mild 8-OHdG expression in hepatocytes of 100 mg of TLE on CMN exposure H and E, Bar: 50 µm.
Effect of tea leaf extract on 8-OHdG expression in gill epithelium. A shows negative 8-OHdG expression in the gill epithelium. B shows severe 8-OHdG expression in the gill epithelium (arrowhead) of CMN exposure. C shows moderate 8-OHdG expression in the gill epithelium (arrowhead) of 50 mg of TLE on CMN exposure. D shows mild 8-OHdG expression in the gill epithelium (arrowhead) of 100 mg of TLE on CMN exposure, H and E, Bar: 50 µm.
Effect of tea leaf extract on Caspase 3 expression in liver hepatocytes. A shows negative Caspase 3 expression in hepatocytes of control fish. B shows severe Caspase 3 expression in hepatocytes (arrowhead) of fish exposed to CMN. C shows effect of moderate Caspase 3 expression in hepatocytes (arrowhead) of TLE (50mg) pretreated fish after exposure to CMN. D shows, mild Caspase 3 expression in hepatocytes (arrowhead) of TLE (100mg) pretreated fish after exposure to CMN, H and E, Bar: 50 µm.
Effect of tea leaf extract on Caspase 3 expression in gill epithelium. A shows negative Caspase 3 expression in epithelium of control fish. B shows severe Caspase 3 expression in the gill epithelium (arrowhead) of fish exposed to CMN. C shows effect of moderate Caspase 3 expression in the gill epithelium (arrowhead) of TLE (50mg) pretreated fish after exposure to CMN. D shows mild Caspase 3 expression in the gill epithelium (arrowhead) of TLE (100mg) pretreated fish after exposure to CMN, H and E, Bar: 50 µm.
