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Molecular Identification of Several Pseudomonas aeruginosa Virulence Genes

Molecular Identification of Several Pseudomonas aeruginosa Virulence Genes

Baraa Qasim Mohammed1, Asmaa Hamoody Abdullah1, Ahmed Raheem Rayshan2* 

1Department of Microbiology, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq; 2Department of Physiology, Pharmacology, and Biochemistry, College of Veterinary Medicine, University of Al-Qadisiyah, Al-Diwaniyah, Iraq.

*Correspondence | Ahmed Raheem Rayshan, Department of Physiology, Pharmacology, and Biochemistry, University of Al-Qadisiyah, Al-Diwaniyah, Iraq; Email: ahmed.rayshan@qu.edu.iq 

Figure 1

The incidence of P. aeruginosa isolated from dog’s ear samples according the area. 

Figure 2

The incidence of P. aeruginosa isolated from dogs ear samples according to date. 

Figure 3

P. aeruginosa isolates on selective and differential media. 

Figure 4

Phospholipase enzyme and hemolysin produce by P. aeruginosa on blood and egg yolk agar.  

Figure 5

Result sheet of VITEK2system for P. aeruginosa. 

Figure 6

The amplification results of 16SrRNA gene of Unknown bacterial species fractionated on 1.5% agarose gel electrophoresis stained with Eth.Br. M: 100bp ladder marker. Lanes 1-6 resemble 1500bp PCR products. 

Figure 7

Agarose gel electrophoresis shows amplification product of 504 bp,153 bp,128 bp fragments of (exoT, oprl, phz+M) gene by PCR. (1.5%) agarose gel (5 volt cm2 1.5 hours). Lane M: DNA ladder (1000 plus) bp. 

Pakistan Journal of Zoology

October

Pakistan J. Zool., Vol. 56, Iss. 5, pp. 2001-2500

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