Haemato-Biochemical and Histopathological Effects of Fungicide Pyraclostrobin on Japanese Quail (Coturnix japonica)
Haemato-Biochemical and Histopathological Effects of Fungicide Pyraclostrobin on Japanese Quail (Coturnix japonica)
Nabeel Ahmad1, Fuan Qiu2, Iqra Shahid1, Abdul Ghaffar1,*, Yasir Mahmood1, Khalid Mehmood3,*, Habiba Jamil1, Fozia Afzal1, Maryam Mahmood1, Batha Tafuzal1, Mohamed Farouk Elsadek4, Muhammad Usman Saleem5, Sajid Hameed3, and Naeem Akram Malik6
ABSTRACT
The use of different pesticides (including fungicides i.e., pyraclostrobin) is increasing day by day in crop farming for achieving high production from crops which is the major source of environmental hazards. These pesticides including pyraclostrobin are related to humans and animals environment and health. Therefore, the goal of the current study was to ascertain the haematological, serum biochemical and histopathological effects of the organic fungicide pyraclostrobin on Japanese quails. The quails (n= 48) that seemed to be sexually active and mature having 57±3 g weight was randomly and equally (n= 12) divided into control (group A), and treatment groups (B, C, and D). The birds were administered pyraclostrobin orally during first and second weeks @ 15, 30, and 50 mg/kg of body weight to treatment groups B, C, and D respectively. On 7 and 15 days, the samples of blood were drawn and placed in vacutainers for blood and serum analysis, while liver, kidney, and spleen samples were collected for histopathological investigations. The results showed that birds in treatment groups (B, C, and D) were dull, having ruffled feathers, watery droppings, salivated, consuming less feed, and rowed often. The blood analysis indicated that pyraclostrobin had a substantial impact on WBC, RBC, Hb, HCT, MCV, MCH, and MCHC counts in treatment groups. Results revealed that in the treatment group’s WBC count was higher compared to the control group. Compared to the control group, the values for RBCs, Hb, HCT, MCV, MCH, and MCHC fall in the treatment groups. Regarding serum biochemistry the values of AST and ALT were significantly higher (P<0.05) compared to the control group. Histopathological investigations exhibited congestion, fatty change, edema, haemorrhages, atrophy of hepatocytes, and eccentric nuclei of hepatocytes in liver tissue. In kidneys edema, renal tubule degeneration, necrosis of renal tubule, and sporadic haemorrhage were observed. Furthermore, in spleen edema, reduced lymphoid tissues, and degeneration of red and white pulp was visible. Altogether, pyraclostrobin causes clinico-haematological changes including anemia, hematopoietic stress and immune-suppression in birds.
To share on other social networks, click on any share button. What are these?
