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Effect of Chronic Adrenocorticotropine Hormone Treatment on LPS-induced Slight Inflammation via Inhibiting Nuclear Translocation of Glucocorticoid-glucocorticoid Receptor Complex in Lung of Pig

Effect of Chronic Adrenocorticotropine Hormone Treatment on LPS-induced Slight Inflammation via Inhibiting Nuclear Translocation of Glucocorticoid-glucocorticoid Receptor Complex in Lung of Pig

Zhiyuan Sun1, 3, Xuhui Zhang2,*, Xian Li3, Yanping Huang3, Shiyan Sui3, Weifeng Liu3, Guochao Ni3, Xiaozhou Xu1, Jianbo Yang1, Xue Lian1 and Hui Jia1

1Department of Animal Husbandry and Veterinary Medicine, Jiangsu Polytechnic College of Agriculture and Forestry, Jurong 212400, P. R. China
2Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, P. R. China
3Key Laboratory of Animal Physiology & Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, P. R. China
 
Xuhui Zhang and Zhiyuan Sun contributed equally to this work and are considered as co-first authors.

*  Corresponding author: huihui19820131@163.com

Fig. 1.

Effects of LPS/ACTH on serum TNF-α concentration. Pigs were administered LPS injection with or without continuous ACTH pretreatment. TNF-α protein level in serum was determine by ELISA. TNF-α, Tumor necrosis factor; LPS, lipopolysaccharide; ACTH, adrenocorticotropine. Mean values with different letters (a, b, c, or d) are significantly different (P <0.05) from each other. n = 5-6.

Fig. 2.

Expression of TLR2 mRNA (a) TLR4 mRNA (b) in porcine lung tissue. Pigs were administered LPS injection with or without continuous ACTH pretreatment. TNF-α protein level in serum was determine by ELISA. TLR, toll-like receptor 2; LPS, lipopolysaccharide; ACTH, adrenocorticotropine hormone. b-actin was chosen as a reference for normalization in the qPCR and Western blot analyses. Mean values with different letters (a, b, c, or d) are significantly different (P <0.05) from each other. n = 5-6.
 

Fig. 3.

GR mRNA (a) total GR protein (b) cytoplasmic (c) and nuclear (d) GR protein content in porcine lung tissue.

Pakistan Journal of Zoology

February

Vol. 54, Iss. 1, Pages 1-501

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