Comparison of Photochemical Tissue Bonding Using Rose Bengal Dye and Conventional Suturing for Closure of Incisional Cutaneous Wounds in Canine Model
Comparison of Photochemical Tissue Bonding Using Rose Bengal Dye and Conventional Suturing for Closure of Incisional Cutaneous Wounds in Canine Model
Saad Ahmad1, Shahbaz ul Haq1, Shujaat Hussain2, Khurram Ashfaq1, Shahrood Ahmed Siddiqui3,4, Arsalan Khan5*, Abubakar Yameen1, Muhammad Wasim Usmani6, Rafiq Ullah7 and Muhammad Arslan Aslam1*
ABSTRACT
The healing of cutaneous wounds is a fundamental aspect of surgical procedures, with traditional suturing being the most common method of closure. However, novel techniques such as Photochemical Tissue Bonding (PTB) have emerged, offering potential improvements in healing outcomes. This study aimed to compare the efficacy of PTB, utilizing Rose Bengal dye and monochromatic green light, with that of conventional suturing in a canine model. Twenty mongrel canines were divided into two groups: Group A underwent PTB, and Group B received conventional suturing for closure of ventral midline cutaneous incisions. Parameters assessed included healing score, healing time, tensile strength of the healed tissue, and histological characteristics. The PTB group demonstrated superior outcomes in several parameters. Tensile strength was significantly higher in the PTB group (79.06 ± 2.3) compared to the suturing group (72.22 ± 1.5) (p<0.05). Epidermis and dermis thicknesses were also significantly greater in the PTB group (135.2 ± 3.7 µm and 1532 ± 146 µm, respectively) than in the suturing group (p<0.05). Additionally, the PTB group showed a higher collagen content percent (85.8 ± 1.8%) and an improved healing score (2.9 ± 0.07) compared to the suturing group. Most notably, the healing time was significantly shorter in the PTB group (3.2 ± 0.86 days) versus the suturing group (14.3 ±1.91 days) (p<0.05). The findings suggest that PTB is a superior method for cutaneous wound closure in canines compared to conventional suturing, evidenced by enhanced tissue integrity, faster healing times, and improved histological outcomes.
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