Tiansen Li1, Meiling Huang2, Zhen Wang1,Fei Guo3,Hui Zhang1,* and Chuangfu Chen1,*
...this reason omp10 mutant was constructed to examine the impacts of the outer membrane protein 10 (Omp10) on bacterial survival, virulence, phagosome-lysosome fusion, and apoptosis induction, as assessed in appropriate in vitro (cell culture) and animal models. Results showed the omp10 mutant was dramatically attenuated for survival in macrophages and mice than the parent strain 2308. With decrease in the...
Muhammad Naeem1, Bushra Sadia1,2,*, Faisal Saeed Awan1and Muhammad Anjum Zia3
...V9, UV11, UV12 and UV 15 mutants producing halo zones. Percentage blood clot lysis led to screening of mutants UV 2, UV 6, UV 7, UV 9, UV 11, UV 12, UV 15 and EB 3, EB 4, EB 11, EB 13 with hyperactivity as compared to wild bacterial strain. Out of the total 15 isolates of EtBr-treated strains, EB4 gave the maximum enzyme activity (7.3 ± 0.005 U ml-1) with a zone diameter of 6 mm. While UV6 was found to be t...
Muhammad Asim, Muhammad Asif, Muhammad Munir* and Muhammad Aslam**
... and its hypernodulating mutant, NODI-3. There was a significant difference in the percent uptake of 3H-ABA between the two varieties both in inoculated and uninoculated plants. A marked difference in the percent distribution of 3H-ABA between different parts of the plants was observed at different developmental phases of root nodules. Leaves of NODI-3 retained more radioactivity as compared to Williams-82 in inoculated as well as uninoculated plants. The stem...
Amna Waseem, Sikander Ali* and Syeda Wajiha Khalid
...Rhizopus oligosporus mutant strain developed by chemical mutagenesis. The wild-type was simultaneously treated with different concentration of EMS and MMS. Among 13 mutant variants, EM-7 showing maximum lipase activity of 71±3.55 U/g, was selected. Resistance in the hyper producing strain was developed by exposing it with low levels of L-cysteine HCl to protect it from instability. It was observed that under optim...
Maryam Javed*, Farwa Saghir, Naveera Aziz, Maham Saeed, Asif Nadeem and Wasim Shehzad
...and buffalo depicted the mutant form Adenine. Results of this study illustrated the genetic tendency of large domestic bovines towards aggression and attack, so they should be handled with great caution.
... 
Waqas Ali* and Mudasser Habib
...e ability to make escape mutants. ...
Ali Awais, Charassri Nualsri and Watcharin Soonsuwon*
...e panicle length in both mutants were higher than that of control. Several mutants also revealed some notable phenotypic variations in the roots, seeds, panicles and leaf morphology. Phenotypic observations determined that the Dawk Kha 50 has more potential of variability than Dawk Pa-yawm towards EMS. Later selection in the advance generations might be useful to isolate agronomically useful mutant
Bibi Nazia Murtaza1,2, Azhar Qayum3, Shamaila Inayat Nadeem1, Naif Awdh Al-Maliki4, Abdulaziz Alamri4 and Abdul Rauf Shakoori2,5,*
...dimensional structure of mutant proteins were built by Swiss-Model and were further subjected to structural alignment and stability studies by I-Mutant Suite and DUET server. Both variants were predicted as ‘disease causing’ and protein stability analysis revealed p.G138V to be more destabilizing variant than p.E31K. When three-dimensional structures of variants were subjected to molecular docking with GTP, the m...
Ragia S. Mohamed1, Rania F. El Naggar2, Mamdouh. M. Hamoud1, Mohamed M. Hamoud3, Abdulrhman M. Gamal1, Samah E. Laban1, Shimaa A.E. Nasr1, Manal M. Zaki1, ElShaimaa Ismael1, Osama K. Zahran1*
Sajida Sabahat1, Asif Nadeem1,*, Maryam Javed1, Muhammad Yasir Zahoor1, Shahid Nabeel1 and Abu Saeed Hashmi2
Bibi Nazia Murtaza1, Mazhar Saeed Chaudry2, Shamaila Inayat Nadeem1, Muhammad Shahid Nadeem3 and Abdul Rauf Shakoori4,*
...nd relapse in NHL. K ras mutant isoforms, being the negative predictors for prognosis, are vital to analyse before the start of adjuvant therapy. Further studies needed to confirm the functional aspects of rs112445441 and other variants involved in NHL pathogenesis.
... 
Mohammad Aquil Siddiqui1*, Muhammad Tahir Khan1, Ghulam Shah Nizamani1, Shafquat Yasmeen1, Imtiaz Ahmed Khan1, Abdullah Khatri1 and Nighat Seema Soomro2
...evaluate three potential mutant lines of lentil against their parent (M-85) and two check varieties (NIA-Masoor-05 and NIA-Masoor-16). The pooled data of the crop, after two years of evaluation, indicated the earliest maturity in the mutant AEL-40/30 (92.0 days). Plant height was observed to be low in all the studied mutant lines (AEL-40/30, AEL-13/30 and AEL-12/30) against the parent and ...
Ju Zhang1,2, Dong-Sheng Jia3, Shi-Hao Zhao1,2, Xiao-Liang Xie3, Hong Chen4 and Hai-Feng Li4,*
...nation in sir-2.1 mutant nematodes. The antioxidant activity of HGQ was investigated by determining reactive oxygen species (ROS) level, paraquat-stress survival rate, malondialdehyde (MDA) content as well as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities.We observed that HGQ exerted little effect on the growth of wild-type N2 nematodes at concentration < 0.6 mg/ml, and 0.05 mg/ml of the HGQ significantly prolo...
Comparative Analysis of Muscle Development in Zebrafish with Different Intermuscular-Bo nes Patterns
Jian Yang1, Guangxiang Tong2, Zhipeng Sun2, Xianhu Zheng2, Weihua Lv2, Dingchen Cao2, Xiaowen Sun2 and Youyi Kuang2*
...ish (Danio rerio) mutant to analyze the impact of imbs deficiency on adjacent muscle development and growth by examining expression of muscle-specific genes and muscle structure. Five muscle-specific genes including myod, myog, myf5, mef2ca and sox6 were selected to test and verify the expression differences in embryonic development stages (3 hpf, 6 hpf, 12 hpf, 24 hpf, and 72 hpf) and post-embryonic stages (15 dpf, 30...
Wenyou Huang1, Dan Yü1, Song Huang1,2, Jian Xiao2, Ping Qi2, Anhua Song2* and Zhen Huang1*
...ation of a collection of mutants with increased virulence. One mutant, designated, MlUV-40b showed ＃3.3-fold increase in virulence as compared to the wild type parent, with an LC50 = 0.3 ×105 conidia/ml versus 1.1 × 105 conidia/ml, respectively and LT
Dina A. Abdulrahman1, Ayman H. EL-Deeb2, Momtaz A. Shaheen1 & Hussein A. Hussein2
...f any
new or mutant strains and update the used vaccine accordingly.
Methods: Twelve epithelial samples were collected from cattle showing signs suspecting of FMD.
The samples were tested by real time RT-PCR using universal primers for detection of all seven
serotypes of FMDV. The positive-resulted samples were then tested using conventional PCR and pan
FMD primers. The same samples were tested again ...
Iram Liaqat1,*, Safdar Ali Mirza2, Sumera Sajjad3, Shaukat Ali1,*, Muhammad Faiz Qamar4 and Ikram Ul Haq5
...ssed. ΔfliI mutant Salmonella strain was used to transform fliI overproducing plasmid pTrc99A by electroporation. Using vital dyes (Alexaflour 488), visualization of motility was observed in wild type, Δ fliI mutant and fliI complemented strainwhich was further assessed by biofilm formation ability. Swimming, swarming motility alongwith significantly reduced biofilm formation wa...
Mohammad Aquil Siddiqui1*, Muhammad Mahran Aslam1, Mahboob Ali Sial1, Nighat Seema Soomro1, Muhammad Tahir Khan1, Shafquat Yasmeen1, Shumaila Sial2 and Imtiaz Ahmad Khan1
...2012 (R00-125/14) is the mutant line that developed through mutation breeding (Gamma radiation) from Rainbow parent. Initially, the promising mutant line was selected from mutant population and advanced homozygous mutant progenies during 2004-05 for yield evaluation in zonal varietal trials and the national uniform rapeseed yield trials (NURYT). The resu...
Tiara Widyaputri*, Aldryan Cristianto Pratama, Dian Vidiastuti, Yudit Oktanella
...frequency is one and the mutant allele is 0 with a heterozygous genotype frequency of 0% in the population. According to the result, the polymorphism that appears in the CD18 gene by PCR-RFLP method in all samples shows the substitution mutation type G base to C transversion and is not related to the BLAD mutation.
Keywords | Dairy cows, CD18 gene, Restriction enzyme, PCR-RFLP, Friesian Holstein crossbreed
...
Yang Yang1,2, Hong Zhou3, Ji Shao1,2, Xinyi Zhang1,2, Pengpeng Xia1,2, Mingxu Zhou4,5, Qiangde Duan1,2 and Guoqiang Zhu1,2*
...TEC (F18), hcp1 deletion mutant (F18ab△hcp1) and complement strain (F18ab△hcp1/phcp1), their pathogenic differences were compared, and the function of hcp1 was discussed from the aspects of growth curve, biofilm formation, adherence, invasion, cytotoxicity, and so on. The results showed that hcp1 of the T6SS did not influence bacterial growth and biofilm formation. In F18ab△hcp1, bacterial motility, adherence and invasion towards the intestinal porcine e...
Amreen Zahra1*, Mushtaq A. Saleem2, Hasnain Javed3, Muhammad Azmat Ullah Khan4 and Abdul Rauf Shakoori4
...istance and viral escape mutants of human immunodeficiency virus (HIV-1) worldwide. Therefore, a deep molecular insight unveiling the genetic characteristics of the prevailing strains is the need of the hour for successful disease control interventions in endemic countries like Pakistan. Here we present, a detailed molecular and computational analysis of the major genetic constituents, Gag-Pol region of HIV-1 for the very first time in Punjab-Pakistan includin...
Muhammad Ikram Ullah
...redict the effect on the mutant protein. A novel missense mutation c. 2710A>T; p.904Tyr>Ser was detected, and co-segregation analysis was established in the complex neurological family. The pymol analysis detected the loss of hydrogen bonding between Thr at 904 with Arg at 916 in mutant MAN2B1. It is concluded that a novel mutation is identified in MAN2B1 associated with a complex neurological disease. The results indi...
Ahmad Nadeem1,2 and Rubina Arshad1,2,*
...T1 (wild type LPA6), T2 (mutant MLPA6), T3 (wild type LPA6 + mutant MLPA6 consortium) and T4 (un-inoculated control). Inoculated and uninoculated seeds were sown in net house in three replicates. In kabuli variety, maximum increase in plant height (16.7%), root length (5.9%), number of secondary branches (19%), pod number (63.2%) and seed number per plant (75%) was observed in MLPA6 mutant...
Ghulam Akbar1*, Muhammad Anjum Zia1, Amer Jamil1 and Faiz Ahmad Joyia2
...s in women. The wild and mutant strains of Streptococcus mutans were used for this trace element optimization study. There are some salts like KH2PO4, K2HPO4, NaHCO3, CaCO3, CH3COONa.3H2O and FeSO4. 7H2O have been optimized for the production of SK. The K2HPO4 has shown the highest enzymatic activity with UV-Et mutant 680.0 UmL-1 and with CaCO3 UV-Et mutant has been found to give the relat...
Maskur1*, Muhammad Muhsinin1, Sulaiman Ngongu Depamede2
...type ++ and heterozygote mutant carrier G+ with a litter size of 1.56 and 1.65 (Kacang); 1.61 and 1.75 (Boerka), respectively. Meanwhile, FecB mutations produce three genotypes: homozygote wild type ++, heterozygote mutant carrier B+ and homozygote mutant BB with a litter size of 1.00, 1.37, and 1.65 (Kacang); and 1.00, 1.18, and 1.74 (Boerka), respectively.
Keywords | Goat, FecXG,...
Imran Muhammad Siddique1, Naseem Sharif1*, Abdul Ghaffar2, Mudassar Naseer1, Ammara Noreen3, Naseem Akhtar4 and Safeer ud Din5
...e for exploiting natural mutant kinnow strains bearing less seeded fruit of high quality characters through vegetative method of propagation thereby avoiding inevitable heterozygosity and polyembyonic nature of citrus fruits....
Fang Chen
...ffect on the activity of mutant 3’UTR luciferase of PTEN (P>0.05). The miR-4728-3p inhibitor group had less cell migration and invasion, and its apoptosis rate increased (P<0.05). The abnormal expression of miR-4728-3p mediated the apatinib resistance of SCLC by targeting PTEN and regulating PI3 K/AKT pathway....
Muhammad Aijaz1, Nasir Mahmood2, Ghulam Mujtaba3 and Imran Riaz Malik1*
...A was 29% and homozygous mutant AA was 52% in breast cancer patients. However, in healthy individuals, GG allele was found in 70%, GA in 20%, and AA in 10% population. These findings proposed that the IL-10 gene (AA polymorphism) at the -1082 position is most prevalent in local breast cancer patients in Pakistan....
Muhammad Adeel Farooq1, Shaukat Ali1*, Ali Hassan1, Rida Sulayman1, Muhammad Ahsan Kaleem2, Hafsa Shahzad1, Muhammad Summer1, Arooj Latif1, Tahreem Tanveer1
... and cucumber peels. The mutant strains of Bacillus subtilis (BSAA-5 to BSAA-40) and Bacillus licheniformis (BLAA-5 to BLAA-40) were prepared by exposure to UV radiation for 5 to 40 min to synthesize α-amylase via submerged fermentation. Then the crude α-amylase synthesized by these mutant strains was optimized and partially characterized. In contrast to wild and all other mutant
Muhammad Ilyas Riaz1, Masood Rabbani1*, Sohail Raza1, Ali Raza Awan2, Aleena Kokab1 and Iqra Nazir3
...n of ΔpurD deleted mutant by deleting the purD gene which is responsible for de novo purine synthesis. For this, the pUC19-K-UP-DN plasmid cassette was initially constructed using pUC19, kanamycin, and purD upstream and downstream fragments. Then the cassette was electroporated into electro competent Brucella abortus RB51. The ΔpurD mutant was confirmed using PCR analysis. Afterward, the growth kinetics of the
ZAHID FAROOQ1, RUQIA BIBI2, IRFAN BABOO3*, KAMRAN ABBAS4, MUHAMMAD SHAHBAZ5, KHALID
JAVED IQBAL6, DILAWAR HUSSAIN7, MUHAMMAD ABRAR8, ASMA CHAUDHRY9 & TANVEER
HUSSAIN10
...eafowl (four
mutant form; black shoulder, pied, white and common peafowl), freshly
egested fecal samples (n=100) from six different captive facilities were
collected. The prevalence of endoprasites among all the Indian peafowl
ranged from 46.6%-66.7%. The highest prevalence was recorded in black
shoulder peafowl 66.7% followed by pied peafowl (66.1%), white peafowl
(59.1%) and common peafo...
Kristina Morkūnienė*, Rūta Insodaitė, Laimutis Kučinskas, Renata Bižienė
...arr; Thr) of Mblk-1 gene mutant allele A was more common in untreated domestic and wild honey bees (18.90% and 8.14% respectively; p=0.001) compared to treated domestic bees which persistently infected with the disease. Regression analysis showed that recessive AA genotype of this polymorphism significantly reduced the odds for varroosis (odds ratio=0.166, 95% confidence interval = 0,049-0,562, p=0.004). SNP at position 7454459 (Asn → Thr) of the Mblk-1 g...
Rabiea Pervaiz, Shaista Bano*, Sarfraz Ali Tunio, Abdul Nabi Jatt and Aisha Amber Soomro
...pseudomycoides, a colony mutant of a strain showing strong antibacterial activity was obtained by screening/selection method and designated as B. pseudomycoides strain SB138m. The sequence of 16S rRNA gene of both wild type and mutant strains of B. pseudomycoides was comparable. In vitro test for antagonism of B. pseudomycoides strain SB138m against indicator cells showed that the strain contained antibacterial potential. Th...
Elkalamawyl, I.M.; Elhddadl, S.; Swelim2, M.A.; Hamdy2, S.M. and Fahmy3, Hanan A.•
...ign: justify;">Two novel mutants of the hepatitis B virus Surface antigen (HBsAg) were characterized. The mutants were isolated from asymptomatic patients who were found to be persistently positive for both I-BsAg and anti-hepatitis B surface antibody (anti-HBs) and a long-lasting anti-HBc (core) IgM. Polymerase chain reaction (PCR) and sequencing were performed to surface antigen region and revealed two genotype D
El-Sayed • l , Eman H; Mahfouze , Sherin A.; Shaltout ,A. D.; El-Dougdoug3 ,Kh. A. and Sayed1 , R. A.
...ants. Selection of plant mutants is based on morphological and ISSR-PCR markers. The DNA based marker is reliable and reproducible for mutant selection for BBTV and BMV resistance banana plants used in the study. Explants from shoot apical meristem were cultured on MS-medium supplemented with different concentrations of 2, 4-D (2, 4 and 6 mg/l) 6-Benzylaminopurine (6, 7 and 8 mg/l), and Sodium Aide ranging from (1, 2 and 3 m...
B. A. Othman; Kh. A. El-Dougdoug; M. H. Abdel Ghaffar and T. F. El-Arabi
... (parental) and Barseem (mutant), and led to decrease the growth characters and nitrogen content of clover plants. While Barseem IP2 (mutant) was not affected by phages since the growth characters and nitrogen content of clover plants were increased. ...
Uqba Mehmood
...ion. The analysis of Spo mutants (strains which lack Sporulation (Spo) gene or have its inactive form) revealed that many of these strains did not produce extracellular proteases. Some evidence for the involvement of serine proteases in sporulation has been provided by inhibitor studies. Nevertheless variation in germination response of germination defective strains with different nutrients was recorded in ALA system, but overall responses of germination defec...
Muhammad Ilyas Riaz1, Masood Rabbani1*, Sohail Raza1, Ali Raza Awan2, Aleena Kokab1, and Rida Haroon Durrani1
...d B. abortus ΔpurD mutant by deleting the purD gene from the RB51 strain through site-directed mutagenesis. For virulence attenuation comparison with the parent RB51 strain, the constructed mutant was evaluated for attenuation estimation and clearance in BALB/c mice. The B. abortus ΔpurD mutant exhibited significant attenuation of virulence and cleared from the mice spleen afte...
M. Iqbal Ahmad and K. E. Park Dr.
...oltine races sex limited mutants
were also selected for further research....
Eslam Arafa1,2, Hanan M.F. Abdien1*, Mohamed Ali Zain El-Abideen3, Emad Diab2,4, Mahmoud Assad2,5, Mohamed Tarek3, Mohsen M.Z. El-Dimerdash1, Wael K. Elfeil1
...indings confirm that the mutant ARV can skip maternal immunity induced by conventional vaccines due to the mismatch between vaccine strains and circulating variants. It is advisable to conduct further epidemiological research on various circulating genotypes and develop targeted autovaccines to address the reovirus situation in Egyptian flocks effectively.
Keywords: ARV Isolation, Viral arthritis, Running-stunting syndrome, ARV v...
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