Asma Noshad1, Mudassar Iqbal1*, Zafar Iqbal1, Hamida Bibi2, Saifullah3, Salma Bibi4, Hamid Ullah Shah1
...onducted to evaluate the insecticidal activity of the ethyl acetate extracts from fruit bodies, mycelia and fermentation filtrate obtained from Pleurotus ostreatus also known as the oyster mushroom. The percent mortality of Macrosiphum rosae (rose aphids) and the LC50 values were calculated and used as an indicative of insecticidal potential. The maximum mortality of aphids was achieved at 80 µg mL-1
Xiang Nong1,2,3,*, Yao-jun Yang1,2, Guang-you Yang3, Feng-zheng Chen2, Mei Tang1,2 and Gang Wang2
... within 24 h. The insecticidal activity of stigmasterol was weaker than that of ageraphorone compound obtained from E. adenophorum Spreng. Through preliminary separation. However, in the experiments, we found an interesting phenomenon, that is, the dead state of mites treated with stigmasterol was similar to that of mites of the positive control group treated with fenvalerate. Furthermore, the dead state of mites treated with stigmasterol was dif...
Amitava Konar, S. Paul and Kiran A. More
Efficacy of different insecticidal treatment schedules against aphid and whitefly on brinjal
...he efficacy of different insecticidal treatment schedules against aphid and whitefly on brinjal was studied by conducting two field experiments at 'Adisaptagram Block Seed Farm, Hooghly, West Bengal, India in two consecutive kharif seasons i.e., 2010 and 2011. The experiment was laid in RBD with seven treatment schedules and three replications. Among all the treatments soil application of phorate at transplanting, followed by foliar spray with acephate, thiodi...
Goutam Samui and S. Jha
Biology, seasonal incidence and management of Apsylla cistellata Bucton. on mango in West Bengal
...al control (Pruning) and insecticidal control measure were taken up in the district of Murshidabad. In mechanical control, both pruning of 15 cm and 30 cm of affected shoots resulted in the formation of lower number of gall/shoot as compared to control. But pruning at 30c m had been found to be most effective in managing the psyllid gall. In insecticidal control, lowest number of galls were recorded per shoot on the branches...
Temel Gokturk1, Elif Tozlu2,* and Recep Kotan2
... were examined for their insecticidal activities in this study. The studied bio agents were tested by spraying on R. simulans nymphs and adults. B. thuringiensis subsp. kenyae, B. brevis and B. sphaericus GC subgroup D were the most effective on nymphs, whereas B. thuringiensis subsp. kurstakii, P. chlororaphi, and B. brevis were the most effective on adults. Under controlled conditions, mortality ...
Rabia Faiz and Dilara A. Bukhari*
...cuses on determining the insecticidal activity of cyt positive B.t. strains against 3rd instar larvae of mosquito. Larval mortality was noted after 24 h and GCU B.t. 4 (400± 1.15) was found to be the most toxic against 3rd instar larvae of mosquitoes. Spores LC50 values of other isolates were 451± 0.90 (GCU B.t. 1), 511± 0.85 (GCU B.t. 2), 525± .013 (GCU B.t....
Sajid Ali Khan1, Mazhar Hussain Ranjha1, Azhar Abbas Khan2,*, Muhammad Sagheer1, Amjad Abbas1 and Zeshan Hassan2
Zubair Ahmed#, Muhammad Farhanullah Khan, Habiballah Rana*
Toxicological effects of Haloxylon recurvum Bunge ex Boiss (Khar Boti) whole plant extract and novel insecticide chlorantraniliprole against maize weevil, Sitophilus zeamais Motschulsky
...plant extract had
insecticidal potential against the stored grain pest, S. zeamais. Current study
suggests H. recurvum plant extract could be a good alternative against
conventional insecticides....
insecticidal potential against the stored grain pest, S. zeamais. Current study
suggests H. recurvum plant extract could be a good alternative against
conventional insecticides....
Hafiz Muhammad Tahir1*, Zafar Iqbal Khan2, Saira Batool2, Kafeel Ahmad2, Salma Begum2
Residual effect of lambda-cyhalothrin on abundance of insect pollinators in marigold field patch
...d plant before and after insecticidal spray was
recorded. In semi-field experiment, honey bees were exposed to insecticide treated
plants for one hour. The mortality rate of honey bees in the control and insecticide
exposed group was compared. Overall, a significant decline in plant pollinators was
observed after application of lambda-cyhalothrin on the patch of marigold plants.
Lambda-cyhalothrin caused significant mortality...
recorded. In semi-field experiment, honey bees were exposed to insecticide treated
plants for one hour. The mortality rate of honey bees in the control and insecticide
exposed group was compared. Overall, a significant decline in plant pollinators was
observed after application of lambda-cyhalothrin on the patch of marigold plants.
Lambda-cyhalothrin caused significant mortality...
Hafiz Abdul Ghafoor*, Muhammad Afzal, Muhammad Luqman and Muhammad Zeeshan Majeed
...selected novel chemistry insecticidal formulations against 2nd instar nymphs of D. mangiferae using standard twig-dip bioassay method according to Completely Randomized Design. Results showed that both factors i.e. insecticidal treatments (F 9, 245 = 146.90, P < 0.001) and time (F 4, 245 = 445.75, P < 0.01) and their interaction (F 36, 245 = 9.20, P < 0.001) had a significant effect on the mortality of mealybug nymp...
Hafiz Abdul Ghafoor*, Muhammad Afzal, Muhammad Asam Riaz and Muhammad Zeeshan Majeed
... plant species for their insecticidal potential against 2nd instar D. mangiferae individuals. Standard twig-dip method was used for toxicity bioassays according to Completely Randomized Design. Mortality of mealybug individuals varied with plant materials and increased along with the extract concentration and exposure time. Botanical extracts of Azadirachta indica (neem) and Gardenia jasminoides (gardenia) were the most effectiv...
Sadaf Rahim and Mudassar Iqbal*
...timicrobial, herbicidal, insecticidal and other such beneficial activities. Production of such compounds can be increased or decreased depending on growth conditions. For these reasons a Trichoderma harzianum was selected for its growth optimization conditions and extraction of insecticidal compounds effective against two major insects pests including Diuraphis noxia and Tribolium castaneum. The pure fungal strain of Trichod...
Mubasher Ahmad Malik1, Samina Jam Nazeer Ahmad2, Muhammad Jalal Arif1 and Jam Nazeer Ahmad1,*
...ucted to investigate the insecticidal properties of native isolated Plutella xylostella granulovirus (PxGV) and Azadirachta indica (AZA) on mortality and development of P. xylostella under laboratory conditions. Both AZA and PxGV were applied alone and in integrated manners at LC20 and LC50 dose rates. The combination of 349.3 parts per million of AZA and 4.8×104 occlusion bodies per millimeter of PxG...
Erum Yawar Iqbal1*, Ashfaque Ahmed Nahiyoon1, Shahnaz Dawar2 and Shahina Fayyaz1
...ase its metabolites with insecticidal mode of actions in the surrounding culture media. New species proficiency was evaluated in comparison with other indigenous isolates and resulted in most efficient at 30ºC after 24h and proved to have good effectiveness as biological control agent and can be easily used instead of live bacteria in future formulations.
... 
Hamza Rehman*, Nida Ahmad Khan, Asad Ali, Warda Batool and Kanwal Razzaq
Bilal Atta1*, Muhammad Rizwan1, Arshed Makhdoom Sabir1, Muhammad Dildar Gogi2, Muhammad Sabar1, Bakhtawar3, Faizan Ali3 and Mehran Sarwar3
...ernatives. In this study insecticidal efficacy of different plant extracts viz. Ginger (Zingiber officinale), Neem (Azadirachta indica), Clove (Syzygium aromaticum) and Tobacco (Nicotiana tabacum) was compared against T. castaneum infesting stored wheat. The results have suggested that mortality and repellency in T. castaneum increased as the dose rate of crude plant extracts and exposure interval increased. The maximum mortality (86.67%) was achieved with the...
Muhammad Salim1*, Ayhan Gökçe1, Muhammad Nadir Naqqash1 and Orkun Ersoy2
Hafiz Abdul Ghafoor1*, Muhammad Afzal1, Muhammad Luqman2, Muhammad Arshad Javed2, Syed Wasim Hasan3 and Muhammad Zeeshan Majeed1
...erall study results, the insecticidal formulations of spirotetramat, lambda-cyhalothrin and sulfoxaflor in combination with EPF and under-canopy hoeing are recommended to the local citrus growers for an effective control of D. mangiferae infestation. ...
Sajid Amin Sarmad1, Muhammad Zeeshan Majeed1*, Muhammad Luqman1, Muhammad Asam Riaz1, Sohail Ahmed3 and Sylvain Nafiba Ouédraogo2
Development and Laboratory Evaluation of a Slow Release Formulation of Fipronil against Subterranean Termites (Odontotermes obesus Rambur)
...ecomposition and loss of insecticidal molecules active against target pests. This study was aimed to develop and evaluate a slow-release formulation (SRF) of a persistent insecticide (fipronil) against O. obesus. Technical grade insecticide fipronil was applied in the form of cellulose-made pellets made up of compressed powdered sugarcane (Saccharum officinarum L.) bagasse and maize (Zea mays L.) cobs. This pellet formulation was evaluated against worker indiv...
Muhammad Zeeshan Majeed1*, Muhammad Shahzad Akbar1, Muhammad Afzal1, Muhammad Mustaqeem2, Muhammad Luqman3, Ijaz Asghar4 and Muhammada Asam Riaz1
...were evaluated for their insecticidal and repellency potential against the subterranean termite Odontotermes obesus Ramb, a destructive insect pest of wooden infrastructures, agricultural crops, orchards and forest plantations. Standard filter paper disc method was used for both toxicity and repellency bioassays according to completely randomized design. The response of termite workers varied with plant species, botanical concentration and exposure time...
Muhammad Zeeshan Majeed1*, Muhammad Afzal1,Muhammad Asam Riaz1,Kanwer Shahzad Ahmed1, Muhammad Luqman2, Mehar Zubair Shehzad1, Muhammad Bilal Tayyab1, Mujahid Tanvir1 and Saadia Wahid1
...omparative evaluation of insecticidal potential of indigenous ethnomedicinal flora of Soone Valley and surrounding Salt Range of Pakistan. Acetone extracts (10%) of forty plant species were evaluated against Asian citrus psyllid (Diaphorina citri), armyworm (Spodoptera litura), house mosquito (Culex quinquefasciatus) and subterranean termite (Odontotermes obesus) using twig-dip, leaf-dip, aqueous exposure and filter paper-dip bioass...
Muhammad Ayub1*, Munir Hussain2, Sher Ahmad2, Syed Arif Hussain Rizvi3, Shahid Hussain1, Muhammad Qasim2, Muhammad Din2, Muhammad Ishaque Mastoi4 and Tajudin2
Wenyou Huang1, Dan Yü1, Song Huang1,2, Jian Xiao2, Ping Qi2, Anhua Song2* and Zhen Huang1*
...orrelated with increased insecticidal activity in cell-free extracts that could potentially be used directly for insect control.
...
Muneer Abbas1*, Dilbar Hussain2, Muhammad Saleem2, Abdul Ghaffar2, Sohail Abbas3, Niaz Hussain1 and Abdul Ghaffar1
... fly by avoiding harmful insecticidal spray application in order to save natural enemies and environment. Experiment was conducted in three guava, citrus and mango producing districts of Punjab i.e. Faisalabad, Sargodha, Toba Tek Singh with five treatments. Each experimental unit comprised of 1 ha area. Five treatments viz. A=sanitation, B=MAT, C= protein based baits, D= plant extracts and E= A+B+C+D were used during 2015-16. Data of % infested f...
Bushra Baig and Saeeda Yousaf*
...mpact showed the highest insecticidal efficacy against whitefly and aphids. The plots sprayed with mix-biopesticide showed the highest yield of okra and potato crops along with a healthier plant growth. The highest yield of Okra (52 kg) and Potato (272 kg)and their plant growth was recorded in plots sprayed with mix-biopesticides. It is concluded that biopesticide from these two plants could be utilized for their efficacy against various pests of okra and pota...
Hassan Shahzad Khan1, Muhammad Tariq1*, Tariq Mukhtar2 and Asim Gulzar1
Muhammad Irfan Ullah1*, Muhammad Arshad1, Abu Bakar Muhammad Raza1, Nimra Altaf1, Muhammad Afzal2
Muhammad Bilal Tayyab1, Muhammad Zeeshan Majeed1*, Muhammad Asam Riaz1, Muhammad Anjum Aqueel2, Sylvain Nafiba Ouedraogo3, Muhammad Luqman4, Kanwer Shahzad Ahmed1 and Mujahid Tanvir1
Muhammad Zeeshan Majeed1*, Muhammad Irfan Ullah1, Dilbar Hussain2, Muhammad Luqman3, Muhammad Qasim1, Gulfam Yousaf1, Hamza Latif1 and Muhammad Zeeshan1
...ificant impact of tested insecticidal formulations and exposure time on the mortality all insect pest individuals. The most effective differential chemistry insecticides against mango leafhopper, citrus psyllids, mango mealybugs and subterranean termites were nitenpyram, clothianidin, thiamethoxam and imidacloprid (causing 74–79% cumulative corrected mortality), thiamethoxam, imidacloprid, clothianidin and spirotetramat (causing 78–85% cumulative m...
Rabia Bibi1, R. Muhammad Tariq2, Samir A.M. Abdelgaleil3 and Munawwer Rasheed1, 4*
Muhammad Ismail1,*, Abu Bakar Muhammad Raza1, Muhammad Zeeshan Majeed1 and Umair Abbas1 and Riaz Hussain2
Muhammad Rizwan, Abu Bakar Muhammad Raza*, Muhammad Zeeshan Majeed and Muhammad Arshad
... of phytoextracts having insecticidal activity. The main objective of this study was to find out the toxic effect of various plant extracts against D. mangiferae. Three concentrations (i.e. 6.25, 12.5 and 25.0%) of leaf extracts of neem, Azadirachta indica A. Juss (Meliaceae), eucalyptus Eucalyptus camaldulensis Dehnh. (Myrtaceae), datura Datura stramonium L. (Solanaceae), batho Chenopodium album L. (Amaranthaceae) and Indian lemongrass Cymbopogon citratus (DC...
Nosheen Jehajo*, Nasreen Memon, Mansoor Ali Shah and Naheed Shah
...ity), to investigate the insecticidal efficacy of Azadirachta indica (A. Juss) (neem) and Ricinus cummunis (L.) (castor) seeds oil that effect the various biological parameters of C. maculatus fed on Vigna radiata (mung beans) including mortality, oviposition, and adult emergence using three replication for each treatment. Four concentrations viz; (0.25, 0.50, 0.75 and 1%) each of the oil were prepared to observe the contact toxicity and surface protectant eff...
Agha Mushtaque Ahmed1*, Fahad Nazir Khoso1, Ali Zachi Abdulqader Alhilfi2, Sohail Ahmed Otho1, Qurban Ali3, Din Muhammad Soomro1 and Zubair Ahmed Soomro1
...ontext, we evaluated the insecticidal effect of four botanical oils against C. maculatus including neem seeds (Azadirachta indica A. Juss), rocket seeds (Erucas sativa Mill.), cotton seeds (Gossypium hirsutum L.) and mustard seeds (Brassica nigra L.). The effect of these oils was recorded on insect mortality, number of adult insects, seed damage (punctured seeds and weight loss of chickpea seeds), seed germination percentage, root length (cm) and seed vigour i...
Muhammad Shakil Ahmad1, Muhammad Afzal1, Liu Yu Feng2, Muhammad Shahroaz Khan1 and Muhammad Zeeshan Majeed1*
... botanical and microbial insecticidal formulations were evaluated either alone or in binary combinations against 3rd instar larvae of S. litura under laboratory and then under the field conditions. In both trials, insecticidal treatments affected significantly the mean mortality or reduction of S. litura larvae recorded both at two and five days post-treatment. In laboratory bioassay, combinations of flubendiamide and A. ind...
Amina Rahat1, Zahin Anjum1*, Sehlina Zehra2, Fatima Umal Baneen3 and Rabia Chishti1
...potential antibacterial, insecticidal and antifungal activities against different. The research has focused on the potential therapeutic efficacy of AliumSativum, NigelaSativa, and Plantago Ovata in the treatment of microbial infections due to their significant antibacterial activity. Additionally, Different fractions of the A. sativum, P. ovata, N. sativa were prepared anad analyzed for antibacterial, insecticidal and antif...
Arshad Javaid1*, Iqra Haider Khan1, Malik F. H. Ferdosi2, Aneela Anwar3 and Mujahid Manzoor4
...lammatory, cytotoxicity, insecticidal and insect repellant properties as reported in the literature. ...
Nasir Shah1,4, Muhammad Ibrahim1*, Zarnosh Habib2, Kalsoom3 and Zahir Shah4
...to plant extracts having insecticidal properties. Extract of three native plant species (Azadirachta indica, Zataria multiflora, Achillea santolina) and their various concentration (2, 1, and 0.5%) were used for the purpose. Firstly, the artificial diet of fruit flies was subjected to these treatments, while on the other hand chikoo fruits which were used for flies to settle on, were dipped in same concentrations of these plant extracts, and dried under shade ...
Muhammad Yasin1, Azhar Abbas Khan2*, Sana Rubab2 and Sumaira Maqsood3
...d out to investigate the insecticidal properties of Beauveria bassiana (Balsamo) Vuillemin (Ascomycota: Hypocreales) in combination with commercial diatomaceous earth (DE) formulation Inert-PMS alone and in integrate manners against these pests. Beauveria bassiana was applied at 1×106 and 1×107 conidia/ml alone, or in combination with DE at 50 and100 ppm. Adult insects were exposed to the treated wheat for 5, 10 and 15 days at 27±2 ºC a...
AMBASH RIAZ1, SHAHID RAZA2* & HIRA MUBEEN3
...cially insecticides. The insecticidal property of B. thuringiensis comes from the production of Crystal protein. The present study is focused on the cloning and characterization of Cry1AB gene which expresses to produce crystal protein. Cry1AB gene was amplified using specific primers which gave successful PCR results of 3.5 kb long gene amplification. The amplified gene was further verified by inserting it into the expression vector. Furthermore, a computatio...
Muhammad Irfan Ullah1*, Khadija Akram1, Abu Bakar Muhammad Raza1, Muhammad Arshad1, Samina Khalid2, Hamza Latif1, Aqsa Amjad1, Nahdia Perveen1, Emaan Sajid1
...il exhibited the highest insecticidal activity, with a 100% mortality rate for all tested concentrations and time intervals for adult beetles. Clove oil also displayed significant insecticidal activity, resulting in mortality rates between 68.0% to 86.6% for the highest concentration (6.0%). However, lemon and orange oils exhibited the lowest insecticidal activity against adult T. castaneu...
Muhammad Shakil Ahmad1, Muhammad Afzal1, Liu Yu Feng2,
Muhammad Zeeshan Majeed1*, Hina Safdar3, Arif Mehmood1, Shahid Iqbal4 and Muhammad Adnan1
...n-conventional synthetic insecticidal formulations against 3rd instar larvae of S. litura. Bioassay with botanical formulations showed a significant toxicity of oil and extract formulations of neem (Azadirachta indica) causing 70–77% larval mortality in 72 h observation and exhibiting minimum medial lethal concentration (LC50) and time (LT50) values (i.e. 12.32 and 38.01 ppm and 16.67 and 11.68 days, respectively). Among microbial formulations tested, S....
M.Ismail Chaudhry and Ashiq Ahmad
...f this valuable crop. In insecticidal trials, out of 5 test chemicals Lebayucid 50%proved most effective against larvae at 0.05%level and against pupae at 0.1%level. Ekalux gave 92%kill in 0.05%dose and 100% kill in 0.1%dose....
K.M. Siddiqui and Mohammad Pervez
...res and
stratification, insecticidal and fungicidal treatments
on germination of blue pine seed were determined for
different seed sources of this species in these studies.
It was observed that the viability of blue pine seed is
significantly reduced within short period if it is
stored at high temperature. Seed stored in dry cold or
stratified condition retains its viability and maximum
terminations per cant as well as speed of germination
are obtaine...
Hanif Gul and M. Ismail Chaudhry
...ineffective. ...
Bashir Hussain Shah, Hanif Gul and M. Ismail Chaudhry
...ays of treatment. ...
Mohammad Shamsul Hasan Khan
...mation on biology and insecticidal control of nursery pests such as cutworms, army-worms cock-chafers, white ants, plant lice and some important insect pests of forest plantations like defoliators and borers has been comprehensively reviewed. Ekalux, Azodrin, Lebaycid and Bidrin against leaf eating insects and Dieldrin, Aldrin and Heptaclhor against white ants and wood borers have been recommended....
Hina Gul1, Amjad Usman1*, Karishma1 and Seema Zubair2
Muhammad Qasim1, Muhammad Zeeshan Majeed1*, Muhammad Arshad1, Umair Abbas1, Mehar Zubair Shehzad1 and Abu Bakar Muhammad Raza1,2
...directly proportional to insecticidal concentrations and exposure times. Significantly higher mortality was recorded by chlorpyrifos (100.0%) and fipronil (95.0%) at 72 h post-exposure with minimum LC50 values of 1.29 and 2.04%, respectively. Similar trend of effectiveness was exhibited by their LT50 values. Minimum mortality of C. heimi workers was recorded by the formulations of chlorantraniliprole and abamectin. Based on overall study results, it is conclud...
Shawky M Aboelhadid1*, Abdel-Azeem S Abdel-Baki2, Khaled M Hassan3,
Samar M Ibrahium4, Saleh Al-Quraishy5, Ahmed O Hassan6 and Asmaa A Kamel1
...re interest due to their insecticidal activities, low harmfulness, and rapid degradation in the environment. Following this approach, herein we tried to improve the insecticidal activities of Pelargonium graveolens (G) through using the geranium oil in nano-emulsion (GN) form and in combination with sesame seed oil (SO). Different concentrations ranged from 0.313 to 10% were prepared from GN and the combination of G with S a...
Hamayun Arshad1, Muhammad Waris2,3 and Qurratulann Afza Gardner1*
...s reported to exhibit bioinsecticidal property. Here, we aimed to search for local strain of L. sphaericus and test its S-layer protein for toxicity against Culex quinquefasciatus and Anopheles stephensi larvae. For this purpose, we isolated various bacterial strains from our local environment using standard microbiological techniques. Among those isolates, we found novel strain of L. sphaericus Q001, identified by ribotyping and MALDI-biotyping. The 16S ribos...
