Mohammed M. Islam1, 3 *, Damian F. J. Purcell2 and George Kannourakis1,3
... suppression subtractive hybridization that can be adapted to unanimously identify viruses of diverse genetic backgrounds. ...
John G. Bruno
...uch as PCR or gene probe hybridization....
Muhammad Shahzad Ahmed and Dilnawaz Ahmed Gardezi
... its improvement through hybridization is limited because of its flowering and viable fuzz production. New avenues suitable for flowering were explored using 20 genotypes for initiation of breeding program in the country. A site near Bagh district (Arja) Azad Kashmir was found suitable and used for morphological evaluation, phenotypic diversity analysis and their flowering behavior sugarcane under local conditions. Two year data revealed that genotype HSF-242 ...
Nirbhay Kushwaha, Achuit K Singh, Brotati Chattopadhyay and Supriya Chakraborty
Recent advances in geminivirus detection and future perspectives
...ding ELISA, nucleic acid hybridizations like dot blot, squash blot and Southern blot techniques, polymerase chain reaction and other DNA polymerase-mediated assays, and microarray detection. Of these, microarray detection provides the greatest capability for parallel yet specific testing and can be used to detect individual or combinations of viruses with sensitivity comparable to ELISA. Methods based on PCR provide the greatest sensitivity among the listed te...
Yanjie Guo1, Weini Wu1, Hongmei Wang2, Xiao Guo2 and Yongping Xu2,*
...Western blot and in situ hybridization to detect the expression and distribution of IL-18Rα in the inferior mesenteric ganglion of the female goat. Our results showed that IL-18Rα mRNA and proteins are expressed in the IMG, which provides molecular evidence for the study of immune and neuro-modulation in the female reproductive system.
... 
Z. A. Soomro, M.B. Kumbhar, A.S. Larik*, M. Imran** and S.A. Brohi***
...ssive segregants through hybridization is suggested. However, hybridization system, which exploits both fixable and non-fixable components, simultaneously, could be useful in the genetic improvement of yield and yield components in upland cotton....
Oguz Bilgin, Kayihan Z. Korkut, Alpay Balkan, and Ismet Baser*
... spike characters in the hybridization scheme, and the hybrids Kyzyltan91xIDSN261, Kyzyltan91xSvevo, Kyzyltan91xIDSN209, SvevoxZenit and Kyzyltan91xSvevo must be considered for finding out transgressive segregants in late segregations to develop a potential durum wheat variety....
Mubashir Ahmad Khan*, Muhammad Yasin Mirza*, Muhammad Amjad*, Nazakat Nawaz*, Malik Shah Nawaz* and Doulat Baig*
ASSESSMENT OF GENETIC DIVERSITY IN GERMPLASM OF LINSEED ( L.)
... and II could be used in hybridization programme by crossing with accessions of Clusters VII, VIII, IX and X followed by selection in segregating populations....
Maqsood Qamar*, S. Dilanawaz Ahmad Gardazi**, Riaz-ud-Din*, Muhammad Sohail* and Syed Haider Abbas*
PARTIAL RESISTANCE TO STRIPE RUST AND ITS EFFECT ON SUSTAINABILITY OF WHEAT YIELD
...stant cultivars/lines in hybridization programme or their cultivation can considerably reduce yield losses incurred by stripe rust....
Muhammad Ismaeel1,2*, Syed Mehar Ali Shah1, Aziz Ur Rahman1 and Mian Ahmad Raza1
...d for use in future rice hybridization programs while the F1 cross combinations DR-92/DR-83, Bas-6129/Dokri-Bas and Bas-2008/Kashmir-Bas are recommended to develop advance generations and improved rice varieties ...
Juan Augusto Hernández-Rivera1, Jaime Molina-Ochoa2,*, Luis Jorge García-Márquez1, Omar Francisco Prado-Rebolledo1, Rafael Julio Macedo-Barragán1, Arturo César García-Casillas1 and Muhammad Irfan Ullah3
... crossbreeding cattle by hybridization; the body condition score in crossbred dairy cattle; the yields in crossbreds; here is also discussed that even when there have been many crossbreeding in dairy cattle, but when the producer choose a system crossbreeding in dairy cows always mostly contemplate only body weight, body condition score, dry matter intake, and feed efficiency. Is suggested to contemplate other traits like genetic potential, lactation (305 d), ...
Tahsin Razzaq*, Muhammad Fareed Khan and Shahid Iqbal Awan
...iveness of selection and hybridization for enhancement of resistance to NCLB and improvement of yield and yield associated traits and their utilization in future breeding program.
...
Xuhai Wang1,2, Xin Li1, Fangyuan Yuan1, Chaocheng Li1, Bin Jia1* and Song Jiang1*
...ohistochemistry, in-situ hybridization was used to study the relationship and the distribution between miR-216b and IL2RB in the intestine tissue of kazakh sheep with resistant and non-resistant MHC haplotypes after oral infection with E. granulosus eggs. The results demonstrated that miR-216b negatively regulated IL2RB in the cystic echinococcosis intestinal tissues, and they were differentially expressed in the intestine tissue between the resistant s...
E. K. Allam.1; B. A. Othman.1; Hayam S. Abdelkader2; and Noher A. Mahmoud2
...0-5 dilution in dot blot hybridization assay. It was suggested that the inhibitory compounds released during the extraction of RNA constitute a limiting factor for the detection of GFLV in infected vines. Both immunological and molecular detection methods provide tools assisting in the understanding of the epidemiology and diversity of nepoviruses as well as to facilitate resistance breeding, cultivar selection, and development of control strategies....
Om-Hashem M. El Banaa l, A.A. Abou Zeid2, Fawzia I. Moursy3 and Azza, G. Farag
...trus trees. Nucleic acid hybridization using non-radioactive DNA probes through southern blot hybridization technique revealed the sensitivity of this technique for detection of S. citri Saglio et al infected citrus trees....
Yufang Liu1,2, Guiling Cao3, Yujing Xie3 and Mingxing Chu1*
... suppression subtractive hybridization (SSH) technology, and puberty-related differential genes were screened. A total of 184 differentially expressed genes were screened, including 57, 68 and 59 in the AO, BO and EO groups, respectively. There are 22 differentially expressed genes were directly associated to the ovary development. According to the gene function analysis in KOG database, the known genes were clustered into 10 subdivisions in A group, 8 in E gr...
Omaima Khamiss
...y ultrathin sections and hybridization dot blots, gel transfer to follow the probable mechanism especially with the high percentage of genome homology that was over 66% with one of five tested restriction enzymes. These observations suggest an important possible role for recombination in the early evolution and biological characteristics of these two viruses.
...
Hagag, N.M.*•, Arafa, A.*; Shalaby, M. A. ** El-Sanousi, A. A. **and Aly, M. M.
...robe and two steps using hybridization probe) were performed and compared as a diagnostic tool for H5N1 virus detection. All these formats of PCRs appeared within the same specificity for H5 gene detection, while they showed difference in sensitivity as the one step conventional RT-PCR showed to be more sensitive than two steps conventional RT-PCR by 10 folds, one step RRT-PCR TaqMan probe was more sensitive than two steps RRT-PCR TaqMan probe by 10 folds, two...
Zaghloull , A.H.; Mahmoud2, Amal; Hassanl , H.Y.; Hemeida2, A.A.; Nayell , M. A. and Zaghawal , A.A.
...infection using dot-blot hybridization. Thirty six samples were collected from Menoufia Governorate (Berket El-Sabaa, Tokh Tambesha and Salamon regions) and Demiate Governorate and were tested in dot-blot hybridization. BEFV was detected in 24 out of 36 samples (66.7%) as follow: 9/9 (100%) correspond to Berket El-Sabaa and Tokh Tambesha regions and 3/9 (33.3%) correspond to Demiate and Salamon regions. In conclusion, a loca...
El-Dougdougi, Kh. A.; Dawoud2, Rehab A.; Rezk2, A.A. and Sofy3, A.R.*
Nasr-Eldin1 , M. A.; El-Dougdoug2, K. A.; Othman2, B. Ahmedi , Sabah A, and Abdcl-Azizl , S. Il.
...ine plants than dot-blot hybridization, the number Of HSVd-infected grapevine plants were 10 plants, PSTVd were 10 plants and the CEVd was detected with high incidence level in grapevine, where 12 out of 100 grapevine trees analyzed were infected. The frequency of HSVd, CEVd and PSTVd naturally infected grapevine trees recorded different percentages as in single (8.33, 8.33 and 11.11%) respectively, double (11.11, 11.11 and 19.44%) for CEVd+HSVd, CEVd+PSTVd an...
S.A. Sidaros, S.A. El-Kewey , Hala A. Amin;Eman A.H. Khatab , A.A. Emeran l , Samaa Abd El-Khalilk and M.A.S. El-Kady
...lified product. Dot blot hybridization was used to establish the authenticity and specificity to the RT-PCR amplified Products of PMMoV. The coat protein gene of an Egyptian isolate of PMMoV was cloned and sequenced, The sequence contained a full-length ORF coding for the viral CP. It comprises 473 nt and a polypeptide chain of 157 amino acids with a M(r) of 17.27. The comparison of the CP ORF nucleotide sequence of Egyptian isolate (PMMoV-E2) with those...
*Amal Abou El-Ela A., M. A. Amer and Eman A. H. Khatab,
...plant. Nucleic acid spot hybridization assay also was used to confirm identification of the present virus as one of the Potyvirus group using DIG labeled general cDNA probe for detection of potyviruses group, It showed high levels of hybridization signal, whereas no RT-PCR and hybridization were observed with uninfected carnation tissues.
* Amal Abou El-Ela, A.
...ne (RNA-3)- Nucleic acid hybridization was useful for the detection of PNRSV in herbaceous and woody plant tissues. In successful attempt to eliminate the virus from infected dormant rose cuttings by heat therapy resulted in 29.6% virus elemination of (PNRSV).
A.A.Farrag; I.A.M. Ibrahim and, H.M. Mazyad
...on-radioactive molecular hybridization methods. The result showed that it is more sensitive than DASELISA and can be used for large scale detection. PCR product was cloned and sequenced. Comparison between local isolate sequence and other published sequences of the Same virus shows 79.4% - 84% homology....
Nurlan Akhmetsadykov1*, Tanatar Kydyrov2, Moldir Akhmetzhanova1, Gulnazi Akhmetova3, Maxat Berdikulov4
...oassay analysis and blot-hybridization techniques were employed. Obtained results showed that 1mM isopropyl ß-D-1-thiogalactopyranoside induction at 2℃ for 18 hours was the most optimal condition for expression of recombinant GM6 T. evansi antigen. From a 500 ml of growth medium, a total of 3.4 mg of recombinant protein was successfully obtained. A recombinant GM6 antigen of T. evansi encoding a 30 kDa polypeptide was developed, which has diagnosticall...
Shishir Sharma1*, Suk Bahadur Gurung2, Ritesh Kumar Yadav1, Bibek Phulara1 and Laxmi Prasad Joshi1
...ligible parent lines for hybridization purposes utilizing principal component analysis and Ward’s clustering. Correlation assessment revealed a negative relationship between disease parameters and grain yield. According to the principal component analysis, three of the nine main components were significant (eigenvalues > 1) and accounted for 44.60%, 15.30%, and 13.60%, respectively of the total variance. PC1 comprised parameters including percent dise...
