Syed Makhdoom Hussain1,*, Tasneem Hameed1, Muhammad Afzal2, Arshad Javid3, Nosheen Aslam4, Syed Zakir Hussain Shah6, Majid Hussain5, Muhammad Zubair ul Hassan Arsalan1 and Muhammad Mudassar Shahzad1
...Cirrhinus mrigala fingerlings fed sunflower meal based diet. The chromic oxide was added as inert marker in the diets. Three replicate groups of 15 fish (Average weight 5.05±0.013 g fish-1) were fed at the rate of 5% of live wet weigh and feces were collected twice daily. The results of present study showed improved growth performance of Cirrhinus mrigala fingerlings in response to phytase sup...
Faiza Ambreen*1 and Muhammad Javed2
...as estimated for 180-old fingerlings of O. niloticusin a static system and then four sub-lethal concentrations viz. 1/3rd of LC50, 1/4th of LC50, 1/5th of LC50 and 1/6th of LC50 were calculated and fish were exposed to these concentrations, separately in glass aquaria for 70 days at constant laboratory conditions. Peripheral blood erythrocytes were taken on 14...
Muhammad Mudassar Shahzad1*, Syed Makhdoom Hussain1, Farhat Jabeen1, Abdullah Ijaz Hussain 2,Sajjad Ahmad3, Abida Ashraf4,Muhammad Zubair-ul-Hassan Arsalan1
...eral availability to fishfingerlings fed Moringa oleifera leaf meal (MOLM) based test diets. Anti-utritional factors (phytate) are present in plant by-products reduces the bioavailability of minerals to fish, resulting in poor fish erformance. Test ingredient was used as M.oleiferaleaf meal (MOLM) to prepare six experimental diets that were supplemented with graded levels of phytase (0, 300, 600, 900, 1200 and 1500 FTU kg-1). The finge...
Abdul Malik1,2, Ghulam Abbas1,*, Abdul Ghaffar3, Ghulam Dastagir4, Sara Ferrando5, Lorenzo Gallus5, Asad Ali Muhammad1,6, Abdul Jabbar2 and Khalil-ur-Rehman2
... shows that Nile tilapia fingerlings reared from 10‰ - 20‰ was significantly (P <0.05) higher than 25% and 30% salinity levels. Present study suggests that Nile tilapia can be reared up to 20% salinity to get good growth and higher survival rate.
...
Syed Makhdoom Hussain1,*, Muhammad Zubair ul Hassan Arsalan1, Arshad Javid2, Abdullah Ijaz Hussain3, Nosheen Aslam4, Qasim Ali5, Majid Hussain6, Muhammad Masoom ul Hassan Rehan7, Muhammad Mudassar Shahzad1,8, Anam Khalid1 and Danish Riaz1
...lity in L. rohita fingerlings. MOLM was added in the diet by replacing fish meal at the levels of 0%, 10%, 20%, 30% and 40%. Triplicate tanks were used for each treatment having 15 fingerlings. These fingerlings fed at the rate of 5% of live wet weight. Chromic oxide as an inert marker was also added in the diets. Feces from each tank were collected twice a day to estimate the nutri...
Syed Makhdoom Hussain1,*, Nosheen Aslam2, Arshad Javid3, Shehzadi Liaquat1,Muhammad Mudassar Shahzad1, 4, Muhammad Zubair-ul-Hassan Arsalan1 and Muhammad Adnan Khalid1
...reochromis niloticus fingerlings fed canola meal-based diet. Six test diets were prepared by using graded levels of probiotics (Protexin) such as 0, 1, 2, 3, 4 and 5 gKg-1 level in the canola meal based diet. Triplicate tanks were used for each treatment and 15 fingerlings were stocked in each replicate. Fingerlings were fed at the rate of 5 % of live wet weight. Plant meal ...
Muhammad Mudassar Shahzad1,2, Syed Makhdoom Hussain2,*, Farhat Jabeen2, Abdullah Ijaz Hussain3, Arshad Javid4, Muhammad Asrar2 and Muhammad Zubair-ul-Hassan Arsalan2
...1) of phytase. The fingerlings were fed at the rate of 4% of live fish body weight twice a day and faeces were collected from each tank. Present research work was carried out to determine the effect of phytase supplementation on mineral digestibility and carcass composition of Catla catla fingerlings fed MOSM based diets. Phytate in plant by-products decreases the bioavailability of minerals and deposition of nu...
Laiba Shafique1,*, Muhammad Afzal1, Syed Zakir Hussain Shah2, Mehroze Fatima3, Huma Naz4, Qaisra Saddique1
...s in Labeo rohita fingerlings. Five experimental diets were prepared containing organic acid (0%), malic acid (2%), citric acid (2%), formic acid (2%), lactic acid (2%) and designed as “OA1, OA2, OA3, OA4 and OA5”, respectively. Eight weeks experimental trail were performed. Water quality characteristics (dissolved oxygen, pH and temperature) were checked daily throughout the trail. Resu...
Rana Zeeshan Habib1, Muhammad Afzal1, Syed Zakir Hussain Shah2,*, Mahroze Fatima3, Muhammad Bilal4 and Syed Makhdoom Hussain5
...y of Labeo rohita fingerlings fed on canola meal based diet. Nine experimental diets were prepared by supplementing CA (%) and PHY (FTU/kg) at the level of 0, 0 (control); 0, 1000; 0, 2000; 1.5, 0; 1.5, 1000; 1.5, 2000; 3, 0; 3, 1000; 3, 2000 respectively. Chromic oxide was used as inert marker in diets to estimate mineral digestibility. Fish fed on PHY supplemented diet showed higher apparent digestibility coefficient (ADC%) of Ca, P, Na, K, Mg, Fe, Cu...
Majid Hussain1, Syed Makhdoom Hussain1,*, Razia Iqbal2, Arshad Javid3, Muhammad Mudassar Shahzad1,4 and Muhammad Zubair-ul-Hassan Arsalan1
...ce of Catla catla fingerlings. MOLM based diet was sub-divided into one control diet (0% CA) and five test diets, each supplemented with 1%, 2%, 3%, 4% and 5% CA, respectively. Chromic oxide as an inert marker was added in the diets to determine nutrient digestibility. The fingerlings were fed twice daily at the rate of 5 % of live wet body weight on their respective diets. Supplementation of CA in MOLM based diets si...
Farkhanda Asad1*, Samina Qamer1, Tayyaba Ali1, Ammara Behzad1 and Tahira Yasmin2
...corn in Cirrhinusmrigala fingerlings for 90 days. Using various levels of chromium chloride hexahydrate six test dietsdesignated as; T1(G/0.0, CrCl3.6H2O mg Kg-1), T2(NG/0.0, CrCl3.6H2O mg Kg-1), T3(G/0.2, CrCl3.6H2O mg Kg-1), T4(NG/0.2, CrCl3.6H2O mgKg-1), T5 (G/0.4, CrCl3.6H2O mg Kg-1) and T6(NG/0.4, CrCl3.6H2O mg Kg-1) were prepared. Results showed highest apparent digestibility coefficient (ADC) of nutrientsdry matter, crude lipid and gross energy in test ...
Mahroze Fatima1*, Muhammad Afzal2 and Syed Zakir Hussain Shah3
...E on Labeo rohita fingerlings. Five experimental diets were formulated to contain one control (VE0), two adequate levels of vitamin E supplementation, i.e. 100 and 150 mg/kg diet (VE100, VE150) and two high levels of vitamin E supplementation, i.e. 1000 and 1500 mg/kg diet (VE1000, VE1500). At the onset of feeding trial, 375 fish of nearly uniform initial body weight (3.58±0.04 ...
Syed Makhdoom Hussain1*, Nisar Ahmad1, Azhar Rasul1, Muhammad Mudassar Shahzad2, Muhammad Latif3, Muhammad Zubair Ul Hassan Arsalan1, Muhammad Umair1 and Hafiza Hina Shafqat1
...eters of C. catla fingerlings fed nano- Cr particles supplemented sunflower meal based diets. The experiment was consisted on seven test diets on the basis of supplementation of nano Cr graded levels (0, 0.5, 1, 1.5, 2, 2.5 and 3 mg Kg-1). Chromic oxide was used as an inert marker. Fingerlings were fed at the rate of 5% of their live wet weight. Maximum improvement in the growth performance (weight gain 197...
Maliha Ayub1, Muhammad Javed1, Fariha Latif 1*, Faiza Ambreen2, Komal Sabir1
...s (n=10) of one year old fingerlings of Labeo rohita were exposed to 96-hr LC50 and sub-lethal concentrations (2/3rd,1/4th and 1/5th of LC50) of manganese for a duration of 30 days in the glass aquaria of 70L water capacity. After 30-day exposure, antioxidant (peroxidase) activity was measured in the fish liver, kidney, gills and brain and then compared with control group. The results showed that th...
Laiba Shafique1,*, Muhammad Afzal2, Syed Zakir Hussain Shah3, Mahroze Fatima4, Huma Naz5, Saif ur Rehman1, Youchuan Wei1 and Qingyou Liu1,*
...ain) of C. idella fingerlings showed significant improvement by feeding formic acid and vitamin D3. Result showed that C. idella fed with vitamin D3 increased the crude protein, ash and decreased the fat contents in muscles while same result was observed by supplementation of formic acid. In conclusion, dietary formic acid and vitamin D3 improves the growth performance and muscle proximate analysis for the C. i...
Muhammad Mudassar Shahzad1*, Syed Makhdoom Hussain2, Afia Muhammad Akram1, Arshad Javid3, Majid Hussain5, Syed Zakir Hussain Shah4 and Asma Chaudhary1
...f the Catla catla fingerlings fed Moringa oleifera leaf meal (MOLM) based diet. The phytic acid’s presence in plant by-products decreases the bioavailability of nutrients to fish, resulting in poor fish growth and low nutrient digestibility in the body. Experimental diet was divided into six groups and were supplemented with graded levels (0, 300, 600, 900, 1200 and 1500 FTU kg-1) of phytase. Cr2O3 wa...
Daniel Masood1, Noor Khan1*, Khalid Javed Iqbal2, Sadaf Dogar1, Abdul Hanan1, Sadia Nazir1, Sheeza Bano1, Azra Anwar2, Sameul A.M. Martin3 and Chris J. Secombes3
...n of Labeo rohita fingerlings. L. rohita (average weight 190.25±00g) were stocked randomly in glass aquaria for a 90 day feeding trial. Fish were fed twice daily with four different iso-nitrogenous diets at a feeding level of 3% of total biomass. The diets contained 26% crude protein in which moringa meal substituted soybean meal at 0% (control), 10%, 20% and 30%, with the groups fed these dietsdesignated as T0, T1, T...
Syed Zakir Hussain Shah1*, Muhammad Afzal2, Mahroze Fatima3, Syed Makhdoom Hussain4 and Tanveer Ahmed5
... energy digestibility by fingerlings when fed PHY sprayed diet. Similarly, CA addition in the diet resulted in increased (p<0.05) digestibility of these nutrients. Also, the minerals digestibility was significantly (p<0.05) affected by top spraying of phytase on the diet pellets. Fish having CA acidified diet also showed improved (p<0.05) minerals digestibility performance. Interaction plots showed synergistic effect of bot...
Asma Chaudhary1, Qurat-ul-Ain Ahmad1, Afia Muhammad Akram1, Sadia Roshan2 and Javed Iqbal Qazi3*
...esof Labeo rohita fingerlings challenged to P. fluorescens intraperitoneally appeared in a dose dependent manner with symptoms like hemorrhage, swelling of the belly and anus, scale damage etc. The pathogen challenged fish were administered with Sphingomonas sp.AsCh-P3 (group-G1) and B. aerius AsCh-A7 (group-G2) treated feed in different experimental set ups. Highest Relative Percent Survival (80-90%) was recorded for fish receiving...
Anila Kousar, Muhammad Naeem*, Samrah Masud, Abir Ishtiaq, Zara Naeem and Rabia Iqbal
...ed Farmed Tilapia (GIFT) fingerlings, a developed strain of Nile tilapia (Oreochromis niloticus). Feed preparation criteria was based on cost effectiveness and local availability of cheaper plant protein feed ingredients. Ten specimens were randomly selected from each treatment hapa for evaluating the concentrations of selected essential and non-essential elements in GIFT fingerlings fed with dietary protein levels of 15% (T...
Asma Chaudhary1, Qurat-ul-Ain Ahmad1, Afia Muhammad Akram1, Mehwish Iqtedar2 and Javed Iqbal Qazi3,*
...s of Labeo rohita fingerlings challenged with well-renowned fish pathogenic bacterium Vibrio anguillarum. Positive control-C2 as well as experimental groups was challenged with V. anguillarum intraperitoneally. Probiotic treated feed with antibacterial potential against V. anguillarum was administered to experimental fishes in different groups; G1 (soon after challenge for 30 days), G2 (for 15 days prior and 30 days after challenge)...
Muhammad Javed Iqbal1,*, Muhammad Afzal1, Khalid Abbas1 and Muhammad Shahid2
...e="text-align: justify;">fingerlings. Twelve experiment diets containing four protein levels (24, 26, 28, and 30 %) at three dietary energy levels (2400, 2700, and 3000 kcal/kg) with P/E ratios from 80.00 to 125.00 mg / kcal were evaluated. Each diet was randomly assigned to triplicate groups of seventeen fish averaging 6.5±0.6 g (mean±SD) for 10 wk. The chromic oxide in the diets was added as an additional inert marker to determine the digestibi...
Kashifa Naghma Waheed*, Tariq Rashid, Sikender Hayat, Muhammad Azeem and Sajid Ali Naqvi
...ival rate of Catla catla fingerlings in polyculture system containing Labeo rohita and Cirrhinus mrigala during twelve-week period. The data obtained was subjected to Multivariate analysis of variance (MANOVA) to find out the statistically significant difference (p<0.01) between two treatments for their response to fish growth. The post-hoc test revealed that growth of Major carps was significantly higher with greenhouse as compared to without greenhouse (a...
Syed Makhdoom Hussain1,*, Hina Gohar1, Muhammad Asrar1, Muhammad Mudassar Shahzad2, Azhar Rasul1, Majid Hussain3, Muhammad Zubair ul Hassan Arsalan1, Nisar Ahmad4 and Aqsa Sharif1
...ology of Cyprinus carpio fingerlings. The diets were formulated in such a way that sufficient supply of all required nutrients were ensured for normal fish growth. Collection of feces from each tank was done twice a day. Impact of each treatment on the absorption of minerals, proximate analysis and hematology were determined using standard methods and formulae. Highest minerals absorption (Ca, Na, K, Fe, Cu, P, Mg and Al) was observed in the fish fed at 400mg/...
Nisar Ahmad1, Syed Makhdoom Hussain2,*, Azhar Rasul2, M. Mudassar Shahzad3, Arshad Javid4, Hamda Azmat5, M. Zubair ul Hassan Arsalan2, Sadia Tabassum2 and Bilal Ahmad2
...omposition of C. mrigala fingerlings fed nano-Se particles supplemented sunflower meal based diets. The experiment was consisted on seven test diets on the basis of supplementation of nano Se graded levels (0, 0.5, 1, 1.5, 2, 2.5 and 3 mg/kg). Chromic oxide was added as an inert marker. Fingerlings were fed at the rate of 5% of their live wet weight. Maximum improvement in mineral absorption (Ca, Na, K, Cu, P and Al) was obs...
Nuzhat Naseem, Sajid Abdullah and Sana Aziz*
...le-body of Labeo rohita (fingerlings) fed distiller’s dried grains with solubles based diet, supplemented with phytase. Six different feeds were formulated by supplementing different concentrations of phytase. i.e. D1 without phytase supplementation, D2, D3, D4, D5 and D6 with 250, 500, 750, 1000 and 1250 FTU phytase per kilogram of diet, respectively. An experiment was conducted for 2 months. During this experiment, water quality parameters were also ex...
Hanan A. Abo-State*, Mosaad M. El-Monairy, Yasser A. Hamouda, Hussein M.A. Hassan
... (Oreochromis niloticus) fingerlings diets on growth performance, feed utilization and body composition. Nile tilapia (4.12g fish-1) were fed the basal diet contained 30.36% crude protein and 3879 kcal kg-1 gross energy with different levels of PSO (0.0 (control), 25%, 50% and 75% from the source of vegetable oil that was added to the basal diet). Fish (300 male) were distributed randomly into four groups (4 groups x 3 replicate x 25 fish of each). All groups ...
Majid Hussain1, Syed Makhdoom Hussain2, Razia Iqbal3, M. Mudassar Shahzad4,*, Syed Zakir Hussain Shah3, Afia Muhammad Akram4, Nisar Ahmad5 and Muhammad Zubair ul Hassan Arsalan2
...ces in Cirrhinus mrigala fingerlings. Basal diet was supplemented with 0%, 1%, 2%, 3%, 4% and 5% CA resulting in the formulation of six experimental diets. Ten fingerlings were stocked in tanks in triplicate for each treatment. Feed was given at 5% live wet body weight of fingerlings for 90 days. Results showed that diet acidification with CA significantly (p< 0.05) improved the mineral...
Samia Azad1, Iram Liaqat1*, Riffat Iqbal1 and Uzma Rafi2
...s aquaria having 35 Rohu fingerlings in each. After exposure for six week, sections of intestine, kidney and liver were excised and studied histologically. Results revealed variation in histological changes from mild to severe depending on concentration of exposure. Shrinkage of sub mucosal tissue, enlarged flattened villi, increased apoptosis and degenerated nuclei alongwith missing cytoplasmic boundaries were observed at high concentration of exposure (0.4-1...
Muhammad Mudassar Shahzad1*, Hamna Rashid1, Syed Makhdoom Hussain2, Sana Bashir1, Fatima Khalid1 and Nisar Ahmad3
...mposition of Catla catla fingerlings fed on rapeseed meal-based diet (RSM). Probiotics enhance the effectiveness of feed utilization, compete against pathogens, and provide resistance against diseases. Six experimental diets were formulated by utilizing an RSM-based meal supplemented with graded levels of probiotics such as 0, 1, 2, 3, 4, and 5 g/kg. For the experiment, triplicate tanks were utilized and in each tank 15 (avg. wt. = 6.76 g)
Amber Iqbal1, Mahroze Fatima2, Syed Z. H. Shah3,*, Muhammad Afzal1, Moazama Batool4, Muhammad Bilal5, Naheed Bano6, Ayesha Khizar2 and Sumbal Iqbal7
...min E supplementation on fingerlings of Labeo rohita during refrigerated storage was investigated. Six graded levels of vitamin E, between 0-125 mg/kg vitamin E, were fed to fish. After 60-day feeding trial, fish were sacrificed, fillets were stored in refrigerator at -20°C and analyzed on 0, 15 and 30th day of storage. Upon refrigeration the vitamin E fed fish showed reduced level of Thiobarbituric acid reactive substances (TBARS) at all storage days. The...
Assessment of Dietary Requirement of Vitamin E for Rohu, Labeo rohita Fingerlings Fed Practical Diet
Uzma Zulfiqar1, Mahroze Fatima2, Syed Zakir Hussain Shah3*, Muhammad Afzal1, Shazia Shamas3, Sadia Roshan3, Moazama Batool4 and Muhammad Bilal5
...nt of vitamin E for rohu fingerlings. Six practical diets were fed to fingerlings including one control diet (E0) and five test diets (E25, E50, E75, E100 and E125) supplemented with graded vitamin E levels including 0, 25, 50, 75, 100 and 125 mg/kg. After the completion of trial, fish growth, vitamin E, antioxidant enzymes activities and fatty acids profile was determined. From the results it was concluded that the graded s...
Ghazala Nasreen1, Ali Hussain2, Komal Tayyab1, Muhammad Rashid3 and Sumaira Aslam1*
... was investigated in the fingerlings of Ctenopharyngodon idella in this study. For this purpose, the fingerlings of C. idella were fed on different concentrations of bioflocs bacteria enriched on banana peels) under different diet treatments for a period of 50 days. The diet treatments devised in this study were, T1 (bioflocs only with zero water exchange), T2 (bioflocs only with 10 % weekly water exchange), T3 (bioflocs + 5...
Shafia Riaz1, Khalid Abbas1*, Tanveer Ahmed1,2*, Huma Naz3, Hina Amjad1 and Shahbaz Ahmad1
...formance. Appraisal fish fingerlings were reared in glass aquaria (twenty fish per population) for 60 days under similar environmental conditions. Condition factor (K) and growth parameters (wet weight, fork and total length) were measured fortnightly. The morphometric data were analyzed using SPSS. Genetics characterization was performed by employing five species specific polymorphic microsatellite markers. Overall, the results of growth trial confirmed that ...
Abdelwahab Mohammed Abdelwahab1,2*, Ahmed Almadani1, Ibrahim Almehsen1
...c feeding to red Tilapia fingerlings. The diets were control and biofloc (00.0%, 33.0%, 66.0%, and 100.0%). The diets were fed to red Tilapia fingerlings (14.3 ± 0.15 g) thrice a day. The study lasted fourteen weeks. Feed utilization, growth performance, morphological fish characters, chemical fish composition, flesh color, and blood profiles were determined. The obtained results illustrated that fishmeal replacement ...
Hasina Basharat1, Muhammad Ramzan Ali2*, Aziz Ahmed2, Rehana Kausar2 and Shamim Akhter1
... stocking rate of 10,000 fingerlings per hectare (500 fish/acre). The experiment design was CRD with four treatments (2%, 4%, 6% and 8% feeding level of wet fish body weight daily) having 3 replications. The feeding the fish at higher levels showed higher final weight and weight gain, percent weight gain and specific growth rate. The values of Feed Efficiency (FE) and Food Conversion Ratio (FCR) were best at the lower feeding levels. Although maximum weight ga...
Sadaf Aman1, Fouzia Tabssum2, Ali Hussain3, Shamsa Jabeen1 and Javed Iqbal Qazi1*
...formance of Labeo rohita fingerlings fed with Lactobacillus rhamnosus supplemented plant by products based feeds. The study comprised of two experimental and one control groups. In treatment 1 (T1), fish were fed with soybean meal along with L. rhamnosus. In treatment 2 (T2), fish were fed with sunflower meal along with L. rhamnosus. The control group was served with commercial feed. Initial mean weights of the experimental fish was recorded as 40.00 ± ...
MUHAMMAD MUDASSAR SHAHZAD1*, FATIMA KHALID1, MEHWISH FAHEEM2, SYED ZAKIR HUSSAIN SHAH3, SANA BASHIR1 & SYED MUHAMMAD KHAN1
...ibility in selected fish fingerlings. Graded levels of the prepared feed containing (0, 2, 4, 6, 8, and 10 g/kg) of Tau were fed to C. carpio fingerlings for 60 days. Test diets were prepared using Linseed meal and other ingredients. Highest digestibility of gross energy (72%), crude fat (66%) and crude proteins (73%) was observed in fish at 4 g/kg level of Tau in LSM based diet. Likewise, better absorption of the maj...
Nwafili Sylvanus Anene, Ibinabo Jessica
...experience with eggs and fingerlings mortality. Majority of the respondents (81.63%) strongly agree that eggs mortality experienced in hatchery was due to fungal infection while 40.82% strongly agree that fungal infection was responsible fry/fingerlings losses in the hatchery. In general, the catfish farmers’ perception of causes of eggs and fry/fingerlings mortality were positive (s...
Muhammad Mudassar Shahzad1*, Tehreem Shabbir1, Syed Makhdoom Hussain2, Fatima Yasin1, Humayoun Huma Maqbool1, Aasia Karim3
...treatment having fifteen fingerlings per tank. Fingerlings were fed at the rate of 4% wet weight twice a day for 70 days. The results revealed that C. carpio fingerlings given 20% BM, as a protein source (BM-III) had the most improved carcass composition (crude protein; 17%, crude fat; 8%, gross energy; 3kcal/g, ash; 6% and crude fiber; 2%), immunological indices (WBCs; 7.48×103mm-3)...
Zahir Muhammad1, Muhammad Zubair Anjum1*, Shamim Akhter1, Muhammad Irfan1, Saira Amin1, Yousaf Jamal1, Sharjeel khalid2 and Shakira Ghazanfar2
...loticus). A total of 120 fingerlings were acquired, assigned randomly into 4 groups (n=30/group) received one of four experimental diets each (30% crude protein supplemented with either T1-L. plantarum1×108 cfu), T2-(P. pentosaceus 1×108 cfu), T3-(L. plantarum and P. pentosaceus1×108 cfu) or (T0-(No probiotics) for 60 days in a triplicate manner (n=10/replicate/aquarium of 1 ft3). Growth performance was assessed by final weight (FW) weight ga...
Syed Makhdoom Hussain*, Hafiza Hina Shafqat, Muhammad Faisal, Mahnoor Saleem, Zeeshan Yousaf and Muhammad Amjad
...conducted on Catla catla fingerlings (average weight 6.73±0.04). Total six experimental diets were formulated containing levels I (control), II, III, IV, V and VI. Diet I constituted fish oil (FO), while diets II, III, IV, V and VI were composed of palm oil, corn oil, sunflower oil, canola oil and mixture of all mentioned vegetable oils respectively. Triplicate tanks were used and every tank had 15 fingerlings. Result...
Kanwal Razzaq1*, Mudssar Aslam1, Sana Arif2, Ansa Idrees3 and Fatima Mubashir3
...retention in silver carp fingerlings when fed with diet containing various organic acids. Five experimental diets such as D1 contains no supplemented organic acids, while D2 contain (malic acid 2%), D3 (citric acid 2%), D4 (formic acid 2%) and D5 (lactic acid 2%) were formulated. The feeding trial time was eight weeks. Throughout experiment, water quality parameters comprising of temperature, pH and DO was checked. Results showed that organic acid supplementat...
Huma Naz1*, Sajid Abdullah2, Tanveer Ahmed3*, Khalid Abbas2, Syed Qaswar Ali Shah1, Muhammad Rizwan1, Fareeha Latif4, Adnan Ahmad Qazi1, Muhammad Adeel Hassan5
...cronucleus Assay. Twenty fingerlings of L. rohita was kept in LC50 concentration of CPF+END, (1.95±0.02 μgL-1 for 96 h) for 5 days after acclimatization. The blood sample of 5 fish was collected after 24, 48, 72 and 96 h to see the DNA damage and nuclear anomalies. Results of the current study revealed that damaged nuclei (DN%) and genetic damage index (GDI) in RBCs of L. rohita exposed to insecticides mixture was increased throughout the experiment....
Muhammad Adnan Khalid1, Syed Makhdoom Hussain1*, Shafaqat Ali2,3*, Abdullah Ijaz Hussain4, Muhammad Asrar1, Nisar Ahamd5, Majid Hussain6 and Muhammad Zubair-ul-Hassan Arsalan7
... Triplicate groups of 15 fingerlings were kept in V-shaped tanks. In present study, a trial of 90 days was conducted to assess the effects of different types of biochar on body composition, mineral status and antioxidant activity of Cirrhinus mrigala. At the conclusion of feeding trial, the protein content was highest (18.51±0.13%) in fingerlings fed with poultry waste biochar and lowest (15.35±0.11%) in fish f...
Danish Riaz1,2, Syed Makhdoom Hussain2*, Majid Hussain3, Muhammad Zubair-ul-Hassan Arsalan4 and Eman Naeem2
...upplementing Catla catla fingerlings fed a diet including sunflower meal (SFM) with probiotics (Protexin®) on growth performance, nutritional digestibility, and hematological indicators. Six test diets and one control diet (0 g/kg) were formulated before the beginning of the experiment, with varying amounts of probiotics (0, 0.5, 1, 1.5, 2, 2.5 and 3 g/kg) added to the basal diet. Fish growth performance, nutritional bioavailability, and hematological indi...
Nageen Iqbal1,2*, Abdul Mateen2, Laiba Shafique1,*, Huma Naz3, Saif ur Rehman4, Nouman Nazir2 and Qingyou Liu4
...d of 8 weeks. Ten (n=10) fingerlings of L. rohita were reared per aquaria having capacity of 80-L water. Feed having 100% fish meal was considered as T1 and diets having 30% and 60% replacement of fish meal by bone meal as main protein source was considered as T2 and T3, respectively. Results showed that replacement of 30% and 60% fish meal with bone meal has better effect on growth of L. rohita analysed whole body composition revealed that lower values of tot...
Muhammad Faisal1, Syed Makhdoom Hussain1*, Majid Hussain2,
Muhammad Zubair ul Hassan Arsalan3, Zeeshan Yousaf1 and Muhammad Amjad1
...rformance of Catla catla fingerlings cultured in 6 earthen ponds. In order to formulate experimental diets, MOLM was used as test ingredient. MOLM was used to replace fish meal (FM) in the diet at varying levels of 0%, 10%, 15%, 20%, 25%, and 30%, respectively. There were five experimental diets and one control diet used during the trial. Fingerlings were placed in a cemented pond for fifteen days under laboratory conditions...
Asad Alam1, Asad Ullah2*, Imad Khan2, Yaseen1, Rafiq Ullah1, Tahira Tayyeb1, Muhammad Hanif1, Maiz ur Rahman1, Muhammad Owais Khan1, Fatima Syed3, Raheela Taj3, Shumaila Gul4, Muhammad Sadeeq5 and Muneeb Islam6
...19g in tank third. Total fingerlings (750) stocked were divided in three tanks (dimensions of length was 15feet, height=4.5feet, water level=3.2 feet) with water volume of 17000 liters in each tank. Formulated feed was used with 30% and 40% crude protein (CP) and buffalo meat. Water temperature was maintained at 31oC using heater (500Watts) with thermostat. The results were recorded with significant value in the growth of fish during 4th to 9th weeks. The valu...
A 90-day feeding trial was designated to evaluate how canola meal (CM)-based diet supplemented with phytase (PHY) and citric acid (CA) affects whole-body composition and hematological parameters in Cirrhinus mrigala and Cyprinus carpio fingerlings. Sixteen experimental diets (T1─T16) with varied CA (0 and 2.5%) and PHY (0 and 750 FTU/kg) levels were prepared. The fingerlings were fed at the rate of 5.0% of live fish body mass. Fifteen fish were randomly stocked into triplicate tanks for each of sixteen test diets. Significant (p<0.05) increase in crude protein (CP) and crude fat (CF) was noticed in fish fed T12 (2.5% CA and 750 FTU/kg PHY) diets. Comparison of treatments showed maximum values of RBCs (3.57×106 mm-3 and 3.18×106 mm-3), PLT (83.32 and 80.35), Hb (8.92g/100ml and 8.62g/100ml) and PCV (29.07% and 30.07%) in C. mrigala and C. carpio fingerlings, respectively, when fed with T12 diets. Conclusively, a 50% CM-based diet with 2.5% CA and 750 FTU/kg PHY supplementation, performed better in terms of whole-body composition and hematological parameters in C. mrigala and C. carpio fingerlings.
...gala and Cyprinus carpio fingerlings. Sixteen experimental diets (T1─T16) with varied CA (0 and 2.5%) and PHY (0 and 750 FTU/kg) levels were prepared. The fingerlings were fed at the rate of 5.0% of live fish body mass. Fifteen fish were randomly stocked into triplicate tanks for each of sixteen test diets. Significant (p<0.05) increase in crude protein (CP) and crude fat (CF) was noticed in fish fed T12 (2.5% CA and 75...
Maqsood Jan1, Muhammad Zubair Anjum1*, Zahir Muhammad1, Misbah Farooq1, Muhammad Qayash Khan2 and Shamim Akhter1
...mposition of grass carp (fingerlings). Grass carp is a fresh water fish of edible test and having multiple feed sources. The study was aimed to enhance the production and improve the meat quality of a grass carp in controlled environment. A total of 120 grass carp (fingerlings) were selected and stocked in 12 glass aquaria (n=10 fish in each) in a triplicate manner with four different diet groups D1 (control), D2 (2g of L. r...
Al-Sadik Y. Saleem1, Mohamed Y.I. Youssef1, Fayza A. Ahmed1*, Shereen El. Abdel-Hamid1, Enas N. Said1, 2, Heba S.A. Gharib1, Asmaa I. Abdelaty1
...the optimal Nile tilapia fingerlings’ behaviour and improved their growth rate. Thus, the usage of extracted oils as a feed additive is very essential to increase growth performance and achieve welfare of Nile tilapia fingerlings.
Keywords | Extracted Oils, Behavioural Patterns, Crossing Test, Growth Rate, Welfare
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Muhammad Ramzan Ali1*, Hasina Basharat2, Aziz Ahmed1, Mubeen Fakhar1 and Shamim Akhter2
...evelopment, one thousand fingerlings of Clarias gariepinus were stocked in earthen ponds. The shooter fish were separated and kept in separate ponds of 0.04-hectare area. The mature 100 males and 100 female brooders fish were selected on basis of morphometric characters. Monthly gonadosomatic index of male and female Clarias gariepinus was lower in male as compare to female fish. Milt volume of male African catfish was directly related to its body weight. It w...
Mukhtiar Ali1, Syed Makhdoom Hussain1*, Muhammad Asrar1, Majid Hussain2, Muhammad Zubair ul Hassan Arsalan3, Zeeshan Yousaf1 and Aumme Adeeba Bano1
...position of Labeo rohita fingerlings. Seven experimental diets were formulated with graded levels, 0, 50, 100, 150, 200, 250 and 300 mg/kg of polyphenols supplementation. Each test diet was allocated randomly to triplicate tanks with 15 fingerlings in every tank. These were fed @ 5% of live wet body weight. Results showed that L. rohita fingerlings fed on test diet T4 with dietary suppleme...
Sadaf Aman1, Yung Fu Chang2, Javed Iqbal Qazi1*, Rahman Ullah3* and Farah Aman4
... respose of Labeo rohita fingerlings fed with Lactobacillus rhamnosus supplemented feed. In a completely randomized design fishes were divided into two different treatments based on feeding with and without probiotics in canola meal and corn gluten meal. The control group was fed with commercial feed. Before stocking, all the aquaria were dried for 3 days. One eighty hundred and eighty fish (Labeo rohita) were stocked in 9 aquaria. Twenty fishes were kept in e...
