Muhammad Abubakar1*, Shumaila Manzoor2, Jonas Johansson Wensman3, Emeli Torsson3, Qurban Ali1 and Muhammad Munir4
...haracterization revealed clustering of the PPRV within lineage IV with significant substitutions in the nucleoprotein (NP) gene. Genetic variations within NP gene, and possibly in other proteins which are essentially mediating protective immunity, may explain the extreme infectious nature of the virus and its host-specific pathogenesis. Moreover, understanding the nature of such circulating field viruses is essential to underpin the endemic potential of PPRV a...
Fida Mohammad, O.S. Abdalla, Sheraz Ahmed, Fakharuddin and S. Rajaram
Sameera Akhtar1*, Muhammad Akram Muneer1, Khushi Muhammad1, Muhammad Yasin Tipu1, Muhammad Anees2, Imran Rashid1, Raza-ur-Rehman3 and Irshad Hussain1
...thin F gene revealed its clustering to sub-genotype VIIe. The inferred residue analysis of the virus revealed a number of substitution mutations in the structural and functional domains when compared to the representative strains of each genotype including the vaccine strains (genotype II and III). Interestingly, some of these mutations were found exclusive to the study isolate. Not only do these prime findings improve our understanding about currently circula...
Jun Gao1,2, Liang-Liang Yue3, Xianhuan Jiang1, Liju Ni4, Muhammad Aqeel Ashraf5, Yuxun Zhou,1 Kai Li1,* and Junhua Xiao1,*
.... fortis individuals clustering into one monophyletic lineage. Three main clades are recovered within M. fortis: (1) North group; (2) South group; and (3) GX group. The North Group distributed on the north side of Qinling Mountains-Huaihe river line as well as the South Group was on the south. It suggests this geographic barrier played an important role in differentiation of M. fortis in China. Furthermore, the samples all from Southwest Chin...
Ghulam Abbas1, Muhammad Jawad Asghar1, Muhammad Rizwan2*, Muhammad Akram1, Jaffar Hussain1 and Fiaz Ahmad1
Ahmed Samy1*, Hala Mohamed Nabil Tolba2, Gamelat Kotb Farag3 and Ahmed Abd El Halim1
...enetic analysis revealed clustering of all tested strains within sub-genotype VIg. Three isolates were assessed for pathogenicity based upon the mean death time (MDT), the intracerebral pathogenicity index (ICPI) and the intravenous pathogenicity index (IVPI) in chicken. The ICPI and MDT revealed that all tested isolates were of moderate virulence (Mesogenic) in chickens. Mature chickens showed no clinical signs or death as assessed by IVPI. Pigeon in Egypt re...
Nida Zia1, *, Ayesha Maqbool2, Muhammad Safdar3, Umm-I-Habiba1, Altaf Mehmood4, Muhammad Usman5, Zahid Iqbal6, Javed Iqbal6, Shahid Mehmood6, Amanullah Khan7 and Sajid Umar8
...igh similarity and close clustering with FAdV-4 and FAdV-11 strains isolated from neighboring countries, suggesting geographic and temporal relationships among these strains. This evidence emphasizes the need of further detailed and more systemic approaches to evaluate FAdVs diffusion and characterization to design effective control strategies.
...
Tahir Iqbal1, Umer Rashid1*, Naveed Shahzad2, Amber Afroz1, Muhammad Faheem Malik3 and Muhammad Idrees4
... protein (ORF2) revealed clustering of Pakistani aHEV (Pak aHEV) strains with members of Orthohepevirus B species. However, Pak aHEV strains were highly divergent from other known members within Orthohepevirus B suggesting as novel aHEV strains circulating in the population of layer chickens in the country. Detection of HEV in layer chickens may pose public health risk in context of zoonosis and food borne transmission if aHEV emerges as z...
Detection and Phylogeny of Infectious Bursal Disease Virus (IBDV) during Field Outbreaks in Broilers
Umm-i-Habiba1,*, Ayesha Maqbool2, Muhammad Safdar3, Nida Zia1, Altaf Mehmood4, Muhammad Usman5, Mehwish Sharif6, Amanullah Khan7 and Sajid Umar8
...igh similarity and close clustering with vvIBDV strains isolated from neighboring countries, suggesting geographic and temporal relationships among these strains. Interestingly, one IBDV strain clustered togather with vaccinal IBDV strains and showed 99% sequence homology with attenuated vaccine strains. Our study revealed exclusive circulation of vvIBDV and these evidences emphasize the need of further detailed and more systemic approaches to study IBDV distr...
Jun Yan Bai1*, Xiao Ping Jia2, Xiao Hong Wu1, Guang Lu Li1, Heng Cao1, Xue Yan Fu1 and Kun Peng Shi1
...spectively. According to clustering analysis, Beijing white quail has the closest genetic relationship with Japan quail, partridge, and mallard, which are clustered in one group.
...
Mehtap Bayir*
... tetrapods. Phylogenetic clustering was seen between sod genes in fugu and their orthologs. Finally, highly conserved gene synteny was determined between fugu sod genes and their orthologs from teleost fish and human.
...
Lei Wang1, Lu Yang2, Lei Luo1, Yaqi Gao1*, Jian Gao1 and Ping Liu1
...ified after assembly and clustering. The resulting unigenes were then compared to homologous sequences using NCBI-NR, Swiss-Prot, KOG, Pfam, eggNOG, KEGG, COG, and GO for functional annotation and classification. Some differences in the regularity of distribution and the time of eclosion of the pupa were detected between the southern and the northern populations. We obtained 142.65 Gb of data from transcriptome sequencing and recovered 70,397 unigenes through ...
Hasaan Aziz*, Fariha Altaaf, Attia Bashir, Abdul Basit, Syed Anam Masood Gardazi and Farah Haider
...hically distinct pattern clustering the study strain to clade Asia-2 but clearly distinct from vaccine strains. Though limited and preliminary data, the findings enhance our knowledge towards CDVs circulating in Pakistan. Further studies are much necessary to ascertain genetic characteristics of CDVs over a wide geographical area of the country together exploring the potential reasons of vaccine failure. ...
Muhammad Shaheen
APPLICATION OF LABELLED K MEANS CLUSTERING FOR GIS CONTRACT AUTOMATION
...a sets. Labelled K Means clustering generate labels for the clusters obtained from K Means Clustering which makes this technique more decisive for text data sets. A novel application of Labeled K Means Clustering for automation of technical part of GIS contracts is given in this paper. Geographic Information System (GIS) contract which is signed between GIS service provider and a client re...
Israr Yasin1, Ismail Qureshi1*, Shahjahn Qaimkhani1, Gulam Mustafa Solangi3, Ismail Qaimkhani1, Ismail Brohi1 and Abdul Ahad Soomro2
A Perspective on Household Dairy Farming in District Naushahro Feroze, Sindh, Pakistan
... units) using multistage clustering method. Estimates of the study revealed that 54% of the households possessed buffaloes. Average number of buffaloes was estimated at 4.28 while 376 (63%) of the household reported cattle with average number of 4.38 heads per household. Small numbers of buffalo and cattle bulls were recorded. Average number of 5.55 goats per households was reported by 35% of all the households. On an overall basis, 2,365 liters of buffalo mil...
Jiawei Hong1,2, Mingqiang Chen 1,2, Zhenghua Deng1,2, Youning Li1,2 and Yu Wang1,2*
... genus. The hierarchical clustering heat map analysis and principal component analysis (PCA) showed that the microbial community of the high dosage group (10 mg/L) was clearly different from the other two groups. Overall, enrofloxacin at high dosage of 10 mg/L significantly altered the community diversity of noble scallop. This study characterized the variation regularity of the intestinal microbial of the noble scallop in response to enrofloxacin treatment. T...
Aly M. Abdel-Salam
...e identity indicated the clustering of the two SCBV in a separate branch from
the other known SCBV; indicating the special nature of the Egyptian SCBV isolates.
Close serologic relationship between SCBV and BSV from Egypt was detected. The
Egyptian antiserum of BSV captured SCBV antigens in leaves and insect vectors in
Immunocapture (IC) PCR analysis. The present study represents the first record of
S...
He Yujiao1,2, Xie Huichun1, Lin Gonghua2,3, ZhangTongzuo2,3, Su Jianping2,3 and Du Yurong1*
...on. We also compared the clustering analysis results generated using mtDNA versus microsatellite markers. The most obvious difference between the two markers was reflected in the fact that the populations Yelashan (YLS) and Bangda (BD) represented a separate cluster based on the microsatellite data. Additionally, our studies involving the comparison of the two markers also reveal that a few of the differentiated populations from the middle region have a higher...
Yao Zou, Shien Ren, Miao Xu, Nannan Liang, Xuxin Zhang, Chongxuan Han and Xiaoning Nan
...lax. We suggest that the clustering patterns could be related to climate and geographical factors. Finally, it was found that most of the skull measurements were positively correlated with latitude but negatively correlated with annual mean temperature and precipitation, which follows Bergmann’s rule. This work provides a significant reference value to scholars who apply themselves to pest control and to the classification and identification of zokors us...
Muhammad Shaheen1* and Abdullah2
Muhammad Jamil1*, Kamran Javed1 and Imran Akhtar2
...glomerative hierarchical clustering tools were employed. Twenty-four recently bred upland cotton strains bearing diversified origin were configured in triplicate following Randomized Complete Block Design (RCBD). The data for novel attributes were recorded. Analysis of variance results indicated that a significant level of variation was existing among the strains for disease index, plant population and micronaire value at (p<0.05), while means for all other...
Zulqarnain Haider1*, Muhammad Akhter2, Syed Sultan Ali1, Tahir Latif1, Rana Ahsan Raza Khan1, Awais Riaz1, Tahira Bibi1, Muhammad Ijaz3, Qasim Raza1, Mohsin Ali Raza4, Samina Sarfaraz1, Muhammad Iqbal1 and Muhammad Rafiq1
...orphologic stability and clustering pattern of twenty promising elite basmati rice (Oryza sativa L.) lines including three checks were investigated in advance generation. Triplicated randomized complete block design (RCBD) was used during three consecutive years from 2016 to 2018 and the data were subjected to combined ANOVA, correlation, principal component, GGE biplots in order to demonstrate the stability and performance of these advance Basmati lines. A hi...
Sara Magdy Hashim1, Elshaimaa Ismael2, Mohamed Tarek3, Faten Fathy Mohammed1*, Fatma Amer Abdel Reheem3, Rawhia Esawy Doghaim1
... that H5N8 field isolate clustering in clade 2.3.4.4b. The histopathological hallmarks, in H5N8 naturally infected ducks, included acute inflammatory, necrotic and vascular reactions that involve different tissues. The immunohistochemical characterization of viral antigen revealed direct relation between viral residence in tissue and developed pathology. The present study confirmed that H5N8 HPAI clade 2.3.4.4b, became a predominant strain during the period 20...
Yong Bin Zhao1, Hui Song1, Guo Gong Yi1*, Xi Lian Hao1, Bai Wang2, Zhi Wen Chen2
... out by using the system clustering method. The results showed a significant difference between the isotope values of the rachis and barbules from the same feather. In addition, the isotope values of the rachises and barbules differed between individuals, although the food sources of the samples might be slightly different. Based on the above results, the cluster analysis was able to distinguish individuals, with the highest accuracy of 85%; the more factors t...
Edwin Ormachea V1,2*, Bilo Calsin C1,2, Eyner Aguilar S3, Buenaventura Ormachea V4, Henry Gonzales C1, Yecenia M. Masias G5
...words | Zoometry, sheep, clustering analysis, morphology; correlation
... 
SHEIKH AJAZ RASOOL1*, MUNAZZA DANISH AJAZ2, MUHAMMAD SALMAN RASOOL3, AHSAN SATTAR SHEIKH4 & FIRDAUS KOUSAR BUKHARI5
...nts in humans and for bioclustering in the poultry houses and animals....
M. Azalou1,3*, C. C. Kpomasse1, A. S. Assani3,4, I. T. Alkoiret3,4, Wéré Pitala1,2
...d ascending hierarchical clustering (AHC) identified three groups of goose farmers. Group 1 was composed of primary and secondary level goose farmers whose main activity was trade (56.1%); group 2 was composed of secondary level herders whose main activity was agriculture (59.38%); and group 3 included public servants (27.59%) with university-level education. As for the feeding of the geese, Group 3 fed more feed than Groups 1 and 2, where the majority of farm...
A. A. El-Kholy1, S. Vilcek2 and A. M. Daoud1
Habibeh Jabbari
... 28S rDNA D2-D3, a clade clustering the four populations of cabbage cyst nematode was formed along with the sequences of H. cruciferae populations deposited in NCBI. These results showed morphometrical, morphological and molecular characters of under study populations are same and the host is the only differences between pathogenicity populations....
Shishir Sharma1*, Suk Bahadur Gurung2, Ritesh Kumar Yadav1, Bibek Phulara1 and Laxmi Prasad Joshi1
...nalysis and Ward’s clustering. Correlation assessment revealed a negative relationship between disease parameters and grain yield. According to the principal component analysis, three of the nine main components were significant (eigenvalues > 1) and accounted for 44.60%, 15.30%, and 13.60%, respectively of the total variance. PC1 comprised parameters including percent disease index (PDI), area under the disease progress curve (AUDPC), disease inciden...
M. Daud AK1,2*, Mahdi Abrar2*, Teuku Reza Ferasyi3,4, Harapan Harapan5,6,7,8
...etermine the spatial and clustering pattern of FMD. The hotspot and autocorrelation analysis with Moran’s I statistic were conducted to validate the spatial patterns. In 2022, the month with the greatest number of instances was July, followed by June, May, August, September, October, and December. There have been no recorded cases of FMD from January to August 2023. The Aceh Besar district was notably recognized as a prominent area with a high incidence ...
Ahmed E. Orbano1, Mohsen El Dimerdash1, Mohamed Abaza2, Ali Zanaty3, Mohamed Rady3, Mohamed H. Nemr3, Wael K. Elfeil1*
...alysis also showed close clustering with TCO vaccine strains, highlighting the genetic stability of the virus. These findings highlight the need for improved biosecurity, vaccination strategies, and continuous molecular surveillance to control respiratory diseases in Egyptian poultry.
Keywords: Respiratory viral infection, Broilers, ILT, Mixed infection
... 
