Mehwish Faheem1,*, Saba Khaliq2 and Khalid Parvez Lone2
...096">Catla catla was exposed to graded concentrations of bisphenol-A (10,100,1000μg/l) for 14 days. BPA exposure strongly elevates vtg mRNA level in fish exposed to 100μg/l but at 1000 μg/l exposure of BPA, vtg level decreased compared to 100µg/l exposed fish. These results showed that BPA has estrogenic action and cause endocrine disruption in juvenile C. catla at enviro...
Muhammad Mudassar Shahzad1*, Syed Makhdoom Hussain1, Farhat Jabeen1, Abdullah Ijaz Hussain 2,Sajjad Ahmad3, Abida Ashraf4,Muhammad Zubair-ul-Hassan Arsalan1
Muhammad Mudassar Shahzad1,2, Syed Makhdoom Hussain2,*, Farhat Jabeen2, Abdullah Ijaz Hussain3, Arshad Javid4, Muhammad Asrar2 and Muhammad Zubair-ul-Hassan Arsalan2
...096">Catla catla fingerlings fed MOSM based diets. Phytate in plant by-products decreases the bioavailability of minerals and deposition of nutrients in fish body, resulting in poor fish growth. Results demonstrated that phytase supplementation showed significant (p<0.05) improvement in ADC% of minerals and carcass composition of fish. Maximum digestibility of minerals and improved carcass composition of C. ...
Majid Hussain1, Syed Makhdoom Hussain1,*, Razia Iqbal2, Arshad Javid3, Muhammad Mudassar Shahzad1,4 and Muhammad Zubair-ul-Hassan Arsalan1
...096">Catla catla fingerlings. MOLM based diet was sub-divided into one control diet (0% CA) and five test diets, each supplemented with 1%, 2%, 3%, 4% and 5% CA, respectively. Chromic oxide as an inert marker was added in the diets to determine nutrient digestibility. The fingerlings were fed twice daily at the rate of 5 % of live wet body weight on their respective diets. Supplementation of CA in MOLM based diets significantly (p< ...
Shafaq Fatima*, Sumrin Sahar and Khalid Lone
...096">Catla catla) is the most important commercial carp in South Asia due to its higher growth, flesh quality and increased market demand. Present study investigated the seasonal variations in gonadosomatic index (GSI) and profiles of sex steroids such as testosterone (T), 11-ketotestosterone (11-KT), estradiol-17β (E2), cortisol (C), progesterone (P) and 17α-hydroxyprogesterone (17α-HP) during annual reproductive ...
Hamda Azmat1, Waqas Ali2, Arshad Javid2, Ali Hussain2, Syed Makhdoom Hussain3, Zara Saeed1,Syed Shafiq Hussain Bukhari4
Chromium contamination in water, sediment and its bioaccumulation in Indian major carps in River Chenab, Pakistan
...096">Catla catla, Labeo rohita and Cirrhinus mrigala was conducted from January through May, 2015. Water, sediment and fish samples were collected on monthly basis from upstream and downstream of head Trimmu, river Chenab. Throughout the study period, mmaximum Cr concentrations from upstream and downstream were recorded as 23.54± 3.43 mg/L and 21.12 ± 3.47 mg/L, respectively. Average metal concentration detected from sediment sample...
Studies on amylase and lipase activity in fishes fed with diet containing different feed ingredients
Mirfa Murtaza1, Sajid Abdullah1, Wardah Hassan1*, Khalid Abbas1, Huma Naz1, Muhammad Anjum Zia2
Studies on amylase and lipase activity in fishes fed with diet containing different feed ingredients
...096">Catla catla and Cyprinus carpio fed
with different feed ingredients. There were two glass aquaria (3×2×2 ft) for each species and ten fish in each
aquarium. Fish in one aquarium were fed on rice polish and fish in second aquarium were fed on corn gluten @ 3 %
of their wet biomass for 70 days. Results showed that fish wet weight varied significantly between the rice polish and
corn gluten fed fishes. Th...
with different feed ingredients. There were two glass aquaria (3×2×2 ft) for each species and ten fish in each
aquarium. Fish in one aquarium were fed on rice polish and fish in second aquarium were fed on corn gluten @ 3 %
of their wet biomass for 70 days. Results showed that fish wet weight varied significantly between the rice polish and
corn gluten fed fishes. Th...
Abir Ishtiaq, Muhammad Naeem*
Length-weight relationships and condition factor for farmed Catla catla (Hamilton, 1822) from southern Punjab, Pakistan
...096">Catla catla) from farming system. Mean±S.E. total
length (TL) and body weight (W) of studied samples have been calculated as 39.29±0.23 cm and 827.43±14.55 g,
respectively. For LWRs, specimens of C.catla were collected from fish ponds and grouped into three categories on
the basis of the feed offered to them which contained 15%, 20% and 25% crude protein (CP). The ...
length (TL) and body weight (W) of studied samples have been calculated as 39.29±0.23 cm and 827.43±14.55 g,
respectively. For LWRs, specimens of C.catla were collected from fish ponds and grouped into three categories on
the basis of the feed offered to them which contained 15%, 20% and 25% crude protein (CP). The ...
Jhan Zeb, Muhammad Javed
Forecasting percentage contribution of plankton biomass towards increase in fish yield under composite culture conditions
...096">Catla catla, Cirrhina mrigala, Ctenopharyngodon idella and
Hypophthalmichthys molitrix in the ratio of 27:10:10:07:13, respectively. Each triplicate group of fish T1 to T6 were fed
supplementary diets at varying protein level viz., 22, 24, 26, 28, 30 and 32% digestible protein (DP), respectively @
2% of their wet body weight daily. However, control fish (T7) although had an access to plankton; but were devoid of
t...
Hypophthalmichthys molitrix in the ratio of 27:10:10:07:13, respectively. Each triplicate group of fish T1 to T6 were fed
supplementary diets at varying protein level viz., 22, 24, 26, 28, 30 and 32% digestible protein (DP), respectively @
2% of their wet body weight daily. However, control fish (T7) although had an access to plankton; but were devoid of
t...
Fariha Latif* and Muhammad Javed
...096">Catla catla, Labeo rohita and Cirrhina mrigala towards metals mixture (Cd+Cr+Cu+Pb) were determined during present research work. Sensitivity of the three fish species (120-day old) towards metals mixture was measured in term of 96 h LC50 and lethal concentration. These acute toxicity tests were performed at constant temperature (30oC), pH (8) and total hardness (250 mgL-1). All the three fi...
Muhammad Ahmad, Zohaib Noor*, Karim Johar Khan, Waqar Younas and Ajmal Hussain
...096">Catla catla (C. catla) a surface feeder, Labeo Rohita (L. rohita) a column feeder and Cirrhinus mrigala (C. mrigala) bottom feeder, respectively, while, in group 2 only (C. catla) was replaced with (Hypophthalmichthys molitrix) H. molitrix as a surface feeder keeping other species the same as in group 1. At the ...
Huma Naz1*, Sajid Abdullah2, Khalid Abbas2, Muhammad Rizwan Tariq3, Laiba Shafique4, Ghazala Nazeer5
...096">Catla catla was computed. The genotoxicity of END+CPF mixture in RBCs of fish was also evaluated by comet assay. To check the genotoxicity of END+CPF mixture, blood of fish was sampled after 24-hr of intervals. Some fishes were also kept in clean water known as negative control (NC) and for positive control (PC) cyclophosphamide was injected into fish. The tolerance limits of C. catla against EN...
Syed Makhdoom Hussain1*, Nisar Ahmad1, Azhar Rasul1, Muhammad Mudassar Shahzad2, Muhammad Latif3, Muhammad Zubair Ul Hassan Arsalan1, Muhammad Umair1 and Hafiza Hina Shafqat1
...ical parameters of C. catla fingerlings fed nano- Cr particles supplemented sunflower meal based diets. The experiment was consisted on seven test diets on the basis of supplementation of nano Cr graded levels (0, 0.5, 1, 1.5, 2, 2.5 and 3 mg Kg-1). Chromic oxide was used as an inert marker. Fingerlings were fed at the rate of 5% of their live wet weight. Maximum improvement in the growth performance (weight gain 197%, FCR 1.49, SGR 1.21 and ...
Abir Ishtiaq and Muhammad Naeem*
...096">Catla catla (Thaila) in a polyculture system was conducted. Four experimental diets containing graded levels of protein (15, 20, 25 and 30%) were tested. Triplicate groups of C. catla stocked in outdoors earthen ponds at 2000 fish/acre were fed at 4% of body weight, 2 times a day, for 180 days. The investigation was spread over three different sites, each providing four earthen ponds. The results revealed ...
Muhammad Mudassar Shahzad1*, Syed Makhdoom Hussain2, Afia Muhammad Akram1, Arshad Javid3, Majid Hussain5, Syed Zakir Hussain Shah4 and Asma Chaudhary1
...096">Catla catla fingerlings fed Moringa oleifera leaf meal (MOLM) based diet. The phytic acid’s presence in plant by-products decreases the bioavailability of nutrients to fish, resulting in poor fish growth and low nutrient digestibility in the body. Experimental diet was divided into six groups and were supplemented with graded levels (0, 300, 600, 900, 1200 and 1500 FTU kg-1) of phytase. Cr2O...
Nosheen1, Sajid Abdullah1, Huma Naz2* and Khalid Abbas1
...096">Catla catla exposed for 4-day. After each sampling (24-hr interval) fish were dissected and required organs were obtained. Results showed that the POD activity was augmented in all selected organs of Pb+2 exposed fish in relation to control. The POD activity in organs of fish followed the order: brain>liver>gills>kidney>heart>muscle. Regression analysis showed a positive relationship between POD activity and duration of exposu...
Mehwish Faheem1*, Maleeha Rafeeq1, Aisha Majeed1 and Saba Khaliq2
Shahid Sherzada1,2,4,*, Muhammad Naeem Khan4, Masroor Ellahi Babar2, Muhammad Idrees3, Abdul Wajid2, Muhammad Nauman Sharif3, Muhammad Shahzad Iqbal3, Iram Amin3 and Muhammad Shahid3
...096">Catla catla)are very suitable fish species for culture in Pakistan. However, the identification and phylogeny of these fish species are of much interest today. DNA barcoding is used as a bio-identification tool for the organism globally. The current study was also conducted for the fast and accurate identification of Indian major carp species. The fishes used in this study were captured from four geographically isolated districts of Punj...
Hafiz Abdullah Shakir1*, Javed Iqbal Qazi2, Abdul Shakoor Chaudhry2, Muhammad Irfan3, Muhammad Khan1, Shaukat Ali4 and Saima Shahzad Mirza5
...096">Catla catla (thaila), Labeo rohita (rohu) and Cirrhinus mrigala (mori)) from river Ravi during two flow seasons at four sampling locations including upstream Lahore Siphon = A, Shahdera = B, Sunder=C and downstream Balloki headworks = D. All the metal contents in fish scales were highly significantly different (P<0.001) among sampling locations and flow seasons. Location-wise metal accumulation pattern was C > D > B > A. The high...
Rabia Iqbal, Muhammad Naeem*, Samrah Masud and Abir Ishtiaq
...096">Catla catla ♂) by feeding them at three graded protein feeds (15%CP, 20%CP, 25%CP). Eighteen days old fry of hybrid (L. rohita ♀ and C. catla ♂) of 0.12±0.08 average weight(g) and length(cm) 1.63±0.21 were acclimatized and shifted in hapas (8x6x3 ft.), fed at the rate of 5% of their wet body weight. Ten samples from each hapa were randomly selected at the end of three months (90 days tr...
Muhammad Khalid1,2, Samrah Masud2, Zara Naeem2, Muhammad Munir Shahid3, Ammar Danyal Naeem2, Abir Ishtiaq2 and Muhammad Naeem2*
Length-weight and Length-length Relationships of Farmed Catla catla during Winter Season from Muzaffargarh, Pakistan
...096">Catla catla, thirty fish specimens were collected from a fish farm located at Muzaffargarh, Pakistan during January, 2019. Total length (TL), standard length (SL), fork length (FL) and body weight was taken of each fish specimen. Mean TL and body weight (W) of studied fish specimens of C. catla was found 49.14±1.58 cm and 1775.29±191.18 g, respectively. Results of regression analyses revealed...
Rabia Iqbal and Muhammad Naeem *
...096">Catla catla ♂). The hybrid fry of mean 1.05±0.08 g body weight and 4.36±0.40 cm mean length were acclimatized and transferred to 8 X 6 X 3 ft. hapas. Fry were fed with fish meal at the rate of 10 % of body weight during acclimatization period and experimental feed at the rate of 5 % of body weight up to the end of the 90 days experiment in duplicate at the fish farms facility. The growth performance of test fishes was observe...
Md. Abdullah Al Mamun1,2*, Shamima Nasren1,3, Sanjay Singh Rathore1 and Kavalagiriyanahalli Srinivasiah Ramesh1
...ify;">Indian major carp, catla (Catla catla) that showed erratic and lethargic movement were collected from College Fish Farm, Mangalore. We examined a total of 20 catla, found all the fish infested with fish louse, firmly attached to the skin, fins, head, and operculum. Based on the morphology of the specimens, the parasites were identified as Argulus j...
Kashifa Naghma Waheed*, Tariq Rashid, Sikender Hayat, Muhammad Azeem and Sajid Ali Naqvi
...096">Catla catla fingerlings in polyculture system containing Labeo rohita and Cirrhinus mrigala during twelve-week period. The data obtained was subjected to Multivariate analysis of variance (MANOVA) to find out the statistically significant difference (p<0.01) between two treatments for their response to fish growth. The post-hoc test revealed that growth of Major carps was significantly higher with greenhouse as compared to without greenho...
Muhammad Mudassar Shahzad1*, Hamna Rashid1, Syed Makhdoom Hussain2, Sana Bashir1, Fatima Khalid1 and Nisar Ahmad3
...096">Catla catla fingerlings fed on rapeseed meal-based diet (RSM). Probiotics enhance the effectiveness of feed utilization, compete against pathogens, and provide resistance against diseases. Six experimental diets were formulated by utilizing an RSM-based meal supplemented with graded levels of probiotics such as 0, 1, 2, 3, 4, and 5 g/kg. For the experiment, triplicate tanks were utilized and in each tank 15 (avg. wt. = 6.76 g) fingerlings we...
Shafia Riaz1, Khalid Abbas1*, Tanveer Ahmed1,2*, Huma Naz3, Hina Amjad1 and Shahbaz Ahmad1
...096">Catla catla. The different hatchery stocks including Farooqabad (FQB), Lahore (LHR), Faisalabad (FSD), Bhalwal (BHL) and Qadirabad (QBD) assumed as five distinct genotypes were compared for genetic variability in relation to growth performance. Appraisal fish fingerlings were reared in glass aquaria (twenty fish per population) for 60 days under similar environmental conditions. Condition factor (K) and growth parameters (wet weight, fork an...
MARIUM ASLAM, MUHAMMAD JAVED, FAIZA AMBREEN* & FARIHA LATIF
...096">Catla catla. To check the concentration based effect, C. catla were exposed, separately, to 96-hr LC50 of zinc and its sub-lethal concentrations viz. 2/3rd, 1/4th and 1/5th of LC50 for 30 days at constant laboratory conditions. After 30-day, the fish were sacrificed and their liver and kidney were analyzed for peroxidase enzyme activity. Change in activity of peroxidase enzyme in the fish exposed to sub-le...
Jameel-ur-Rehman1, Asghar Ali Kamboh1*, Ahmad Ali Moryani1, Riaz Ahmed Leghari1, Abdullah Sethar2, Ali Raza Nizamani3, Abdul Ahad Soomro3, Kanwar Kumar Malhi1
...096">Catla catla (CC; local name Thaila) and Cirrhinus mrigala (CM; local name Morakhi). The samples were processed for isolation of bacterial organisms using conventional culture method and isolates were tested for antimicrobial sensitivity according to CLSI method. According to results, the prevalence of bacterial organisms was high (p < 0.05) in CC than CM and LR. E.coli were the most common organism in market and pond fish samples with occ...
Moazama Batool1*, Saima Naz2*, Sheeza Bano1, Sadia Nazir3, Ghulam Abbas4, Ahmad Manan Mustafa Chatha5, Maria Lateef2 and Fatima Yasmin2
...096">Catla catla and Labeo rohita, employing probit analysis. Over a period of 90 days, mortality served as the primary toxicity criterion. The experiments were conducted under consistent conditions of pH (7), temperature (28.00.00°C), and water hardness (198.00 mgL-1), with three replicates for each dosage in the tests. Significant variations were noted in the LC50 values and lethal responses for both fish species. C...
Javairia Shafi*, Kashifa Naghma Waheed, Zahid Sharif Mirza and Shaista Razaq
...Lemna minor) in Gibelion catla feed. Four different isonitrogenous feeds (with 0, 10, 20 and 30% replacement of fish meal with) were prepared and fed to juvenile catla for 60 days at the rate of 5% fish biomass. No significant difference (P>0.05) in fish growth parameters and feed efficiency was observed among the treatments. There was no significant difference in apparent digestibility coefficient (ADC) of dry matter amo...
Syed Makhdoom Hussain*, Hafiza Hina Shafqat, Muhammad Faisal, Mahnoor Saleem, Zeeshan Yousaf and Muhammad Amjad
...096">Catla catla fingerlings (average weight 6.73±0.04). Total six experimental diets were formulated containing levels I (control), II, III, IV, V and VI. Diet I constituted fish oil (FO), while diets II, III, IV, V and VI were composed of palm oil, corn oil, sunflower oil, canola oil and mixture of all mentioned vegetable oils respectively. Triplicate tanks were used and every tank had 15 fingerlings. Results demonstrated that significan...
Danish Riaz1,2, Syed Makhdoom Hussain2*, Majid Hussain3, Muhammad Zubair-ul-Hassan Arsalan4 and Eman Naeem2
...096">Catla catla fingerlings fed a diet including sunflower meal (SFM) with probiotics (Protexin®) on growth performance, nutritional digestibility, and hematological indicators. Six test diets and one control diet (0 g/kg) were formulated before the beginning of the experiment, with varying amounts of probiotics (0, 0.5, 1, 1.5, 2, 2.5 and 3 g/kg) added to the basal diet. Fish growth performance, nutritional bioavailability, and hematologica...
Muhammad Faisal1, Syed Makhdoom Hussain1*, Majid Hussain2,
Muhammad Zubair ul Hassan Arsalan3, Zeeshan Yousaf1 and Muhammad Amjad1
...096">Catla catla fingerlings cultured in 6 earthen ponds. In order to formulate experimental diets, MOLM was used as test ingredient. MOLM was used to replace fish meal (FM) in the diet at varying levels of 0%, 10%, 15%, 20%, 25%, and 30%, respectively. There were five experimental diets and one control diet used during the trial. Fingerlings were placed in a cemented pond for fifteen days under laboratory conditions for acclimatization. There we...
