Toxicity and Behavioral Outcomes of the Ethanolic Extract of Schinus Terebinthifolius Raddi fruits in Wistar Rats
Toxicity and Behavioral Outcomes of the Ethanolic Extract of Schinus Terebinthifolius Raddi fruits in Wistar Rats
Soumia Ed-Day1*, Meryam Laanaya1, Chaimae El Kourchi3, Radia Elgui1, Fatima Ezzahra Kacimi2, Latifa Didou1, Samira Boulbaroud2, Hicham Harhar3, Fatima-Zahra Azzaoui1
Scheme of the experimental procedure.
General locomotor activity of rats in the open field test. The number of total squares visited 14 days after the oral administration of the ethanolic extract of Schinus terebinthifolius Raddi Fruits. n: 8, the results are presented as Mean ±SEM. The level of significance is 0.05 (One-way ANOVA and post hoc multiple comparisons).
Anxiety level of rats in the elevated plus maze test, 14 days after oral administration of ethanolic extract of Schinus terebinthifolius Raddi fruits. A. The number of entries in the open arms; B. The time spent in the open arms. n: 8, the results are presented as Mean ±SEM. The level of significance is 0.05 (One-way ANOVA and post hoc multiple comparisons).
Anxiety level of rats in Light-dark box test, 14 days after oral administration of ethanolic extract of Schinus terebinthifolius Raddi fruits; A: Total tenure of time spent in the black chamber; B: Total tenure of time spent in the black chamber; C: Number of entries in the white chamber; n: 8, the results are presented as Mean ±SEM. The level of significance is 0.05 (One-way ANOVA and post hoc multiple comparisons).
Effect of ethanolic extract of Schinus terebinthifolius Raddi fruits on spontaneous alternation in Y-maze test; n: 8, the results are presented as Mean ±SEM. The level of significance is 0.05 (One-way ANOVA and post hoc multiple comparisons).
Effect of the ethanolic extract of Schinus terebinthifolius Raddi fruits on A: The recognition index 2h after training (STM); B: The recognition index 24h after training (LTM); n: 8, the results are presented as Mean ±SEM. The level of significance is 0.05 (One-way ANOVA and post hoc multiple comparisons).
Photomicrographs of liver sections following single oral administration of the ethanolic extract of Schinus terebinthifolius Raddi fruits showing a normal histological structure of the hepatocyte (H), the sinusoid (S), Centrilobular vein (CV), Kiernan’s spaces (KS), Hepatic cell cords (HCC), Portal vein (PV), Portal area (PA), and Binucleate hepatocyte (H and E-stained under 20× magnification power).
Photomicrographs of kidney sections following single oral administration of the ethanolic extract of Schinus terebinthifolius Raddi fruits showing a normal histological structure of the glomerulus (G), distal convoluted tubules (DCT), and Proximal convoluted tubules (PCT). (BV) and (BC) indicate blood vessels and capillaries (HandE-stained under 20× magnification power).
