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Synthesis, Characterization and Investigation of the Anticoccidial Activity of New formulation Curcumin - Olive Oil Nano-Composite

Synthesis, Characterization and Investigation of the Anticoccidial Activity of New formulation Curcumin - Olive Oil Nano-Composite

Reem M. Ramadan1, Fady Sayed Youssef2, Gehad Genidy Mohamed3, Sameh Hamed Ismail4, M.M. El-Bahy1, Shimaa Abdel-Radi1* 

1Department of Parasitology, Faculty of Veterinary Medicine, Cairo University, 1221 Giza, Egypt; 2Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, 1221 Giza, Egypt; 3Chemistry Department, Faculty of Science, Cairo University, 12613 Giza, Egypt and Nanoscience Department, Basic and Applied Sciences Institute, Egypt-Japan University of Science and Technology, New Borg El Arab, Alexandria, 21934, Egypt; 4Faculty of Nanotechnology for postgraduate studies - Cairo University- Sheikh Zayed Branch Campus, Sheikh Zayed City, Giza PO 12588, Egypt.

*Correspondence | Shimaa Abdel-Radi, Department of Parasitology, Faculty of Veterinary Medicine, Cairo University, 1221 Giza, Egypt; Email: shimaa.abdelradi@cu.edu.eg 

Figure 1

SEM and TEM images of the produced C-Oo.Nc 

Figure 4

Variations in the degree of lesion score in chickens inoculated by sporulated oocysts exposed to LC50 of C-Oo.Nc (A) in comparison with non-exposed control oocysts (B) with a marked difference in the level of the lesion in the ceci. 

Figure 5

Comet assay demonstrate the damage in DNA of Eimeria spp. oocysts exposed to C-oo.Nc; (A&B) are control non-exposed oocysts; (C&D) low level of damage recorded in that exposed to 5 & 10 ppm/24h and (E&F) high level of damage in recorded in oocysts exposed to 40 ppm and Amprolium (20%) 240 ppm /24h. 

Eq1
Figure 2

AFM images for (C-Oo.Nc)  

Figure 3

Demonstrating the effect of different C-Oo.Nc doses and exposure time on sporulation inhibition of the exposed immature oocysts as well as calculation of LC50 & LC90.  

Advances in Animal and Veterinary Sciences

May

Vol. 12, Iss. 5, pp. 802-993

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