Genetic Diversity of the CSN2 Gene and its Impact on Milk Production and Quality in Indonesian Friesian Holstein Cattle
Genetic Diversity of the CSN2 Gene and its Impact on Milk Production and Quality in Indonesian Friesian Holstein Cattle
Salsabila1, Siti Kholifah3, Ronny Rachman Noor2, Jakaria2*
ABSTRACT
This study aimed to analyze the diversity of the CSN2 gene in Indonesian FH cattle and test whether the A2A2 genotype impacts milk production and quality, including lactose, protein, and fat levels. 107 blood and 21 milk samples of FH from BBPTU-HPT Baturraden were extracted using the GeneAid kit to produce pure FH DNA isolation. The diversity of the CSN2 gene was analyzed using sequencing. The analysis results, such as genotype frequency, allele frequency, heterozygosity value, and Hardy-Weinberg equilibrium, were calculated using Popgen32. Association between milk production, fat, protein, and lactose content of FH genotype A1A1 and A2A2 using t-test. The results of this study indicate that FH milk production is high compared to several other regions in Indonesia, with high protein and fat content and low lactose based on SNI. The results of the CSN2 gene analysis based on SNP rs43703011 obtained three genotypes: A1A1 (57%), A1A2 (1%), and A2A2 (42%), with an A1 allele frequency of 57% and an A2 of 42%. These genotypes have a Hardy-Weinberg equilibrium in an unbalanced status due to selection. This SNP (A>C; rs43703011) results in a substitution of histidine (A1A1 genotype) with proline (A2A2 genotype). FH cow milk production, lactose, protein, and fat levels with genotype A1A1 were not significantly different (P>0.05) compared to genotype A2A2. It can be concluded that exon 7 of the CSN2 gene is polymorphic in FH cows. However, the A1A1 and A2A2 genotypes of the CSN2 gene are not different in lactose, protein, and fat percentage.
Keywords | Casein (CSN2) gene, Indonesian FH cattle, Milk quality and production, SNPs
To share on other social networks, click on any share button. What are these?
May 2025
Adv. Anim. Vet. Sci., Vol. 13, Iss. 5,
