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Proliferation Capacity and Expression of Cell Cycle Genes in Normal and Gestational Diabetes Affected WJMSCs

Proliferation Capacity and Expression of Cell Cycle Genes in Normal and Gestational Diabetes Affected WJMSCs

Nabila Rasheed1, Shumaila Usman2*, Madeeha Sadiq3, Bushra Wasim3, Kanwal Haneef4, Hafsa Shafqat3, Tabinda Urooj3 and Saman Rashid4
 

1Department of Anatomy,  RYK Medical and Dental College, Rahim Yar Khan, Pakistan
2Department of Molecular Medicine, Ziauddin University, Karachi
3Department of Anatomy, Ziauddin University, Karachi
4Dr. Zafar H. Zaidi Center for Proteomics, University of Karachi, Karachi
 
* Corresponding author: [email protected]

Fig. 1.

Immunocytochemistry of WJMSCs CD90 protein (A) ACTIN (B) and protein showed positive expression for the WJMSCs in cord tissue.

Fig. 2.

Relative genes expression of cell division cycle genes (CDCA2, A8, A20, CCNA2), in control and GDM WJMSCs. There was a significant change in gene expression of two groups. As compared to control, GDM WJMSCs showed highly significant down regulation of cell cycle genes, which confirms the decreased proliferation rate in GDM WJMSCs.

Pakistan Journal of Zoology

October

Pakistan J. Zool., Vol. 56, Iss. 5, pp. 2001-2500

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