PROP-1 Gene a Selective Marker of Wool Production in Sheep Breeds of Balochistan Pakistan
PROP-1 Gene a Selective Marker of Wool Production in Sheep Breeds of Balochistan Pakistan
Chandni Wajid1, Abdul Hameed Baloch1, Ahmed Nawaz Khosa1*, Hubdar Ali Kaleri2, Nasrullah Bangulzai1, Sarfraz Ali Fazlani1, Haleema Sadia3, Saeeda Kalsoom4, Muhammad Bilawal Arain2, Wassem Ali Vistro2
Gel Electrophoresis image showing genomic DNA bands of the samples
Amplified PCR products of exons 1-3.
Insertion of A at the position, c.63 G>A (p.L21L) at exon 1 resultingframe shift mutation in the preceding codons
A Missense heterozygous variation at exon 1, c.85 G>A (p.A29D)
A frame shift mutation at exon 1 c.94 G>T and an insertion of G, changing codon into GGT and TGT resulting frame shift mutation causing change in preceding codons
A frame shift nonsense mutation at exon 2 c.104C>G and an insertion of A resulting codon change from TCG into TCA and TGA (p.S35S and a stop codon)
A silent heterozygous variation at exon 2, c.237 G>A (p.A79A)
A silent homozygous variation at exon 3 c.432T>C (p.S144S)
A silent heterozygous variation at exon 3, c.450C>T (p. P148P)