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Molecular Detection of Leucocytozoon spp. in Broiler Chickens in Baghdad City

Molecular Detection of Leucocytozoon spp. in Broiler Chickens in Baghdad City

Rana Mohammed Ibrahim1*, Haider Mohammed Ali Al-Rubaie2

1Department of Microbiology, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq; 2Department of Parasitology, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq. 

 
*Correspondence | Rana Mohammed Ibrahim, Department of Microbiology, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq; Email: [email protected]

Figure 1:

Thin blood smears Giemsa stained, shows different forms of Leucocytozoon spp. in boiler chickens (X100).

Figure 2:

PCR products (bp503) of electrophoresis in agarose (1.5%) at 5 Volt /cm2 for1:30 hours. Lanes 1 and 7 positive, M: DNA ladder (100). 

Figure 3:

The Leucocytozoon spp. positive results in qRT-PCR (red and violate color) with Cp. Fam. 23.4 and 23.1.

Advances in Animal and Veterinary Sciences

December

Vol. 12, Iss. 12, pp. 2301-2563

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