Molecular Detection of Leucocytozoon spp. in Broiler Chickens in Baghdad City
Molecular Detection of Leucocytozoon spp. in Broiler Chickens in Baghdad City
Rana Mohammed Ibrahim1*, Haider Mohammed Ali Al-Rubaie2
Thin blood smears Giemsa stained, shows different forms of Leucocytozoon spp. in boiler chickens (X100).
PCR products (bp503) of electrophoresis in agarose (1.5%) at 5 Volt /cm2 for1:30 hours. Lanes 1 and 7 positive, M: DNA ladder (100).
The Leucocytozoon spp. positive results in qRT-PCR (red and violate color) with Cp. Fam. 23.4 and 23.1.