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Leverage of Chromium Supplementation on Growth and Blood Profiles in Weaned Rabbits

AAVS_12_12_2356-2363

Research Article

Leverage of Chromium Supplementation on Growth and Blood Profiles in Weaned Rabbits

Efe Peterson Irikefe-Ekeke1, Oghenebrorhie Obakanurhe1*, Adimabua Mike Moemeka1, UfuomaGodstime Sorhue2, Ifeoma Jane Onwumelu1, Ifo Alex Amata2

1Department of Animal Production Dennis Osadebay University, Asaba, Delta State, Nigeria; 2Department of Animal Science, Delta State University, Abraka, Delta State, Nigeria.

Abstract | Animal performance has considerably deteriorated as a result of recent, ongoing consequences of climate change that are raising temperatures. Chromium (Cr), especially when added to animal diets, can halt the lipid peroxidation caused by climate change because of its strong antioxidant qualities. A four-week study to determine the response of weaned rabbits at 5 weeks old on varying levels of organic and inorganic chromium supplementations was conducted. One hundred and twenty-six composite breeds of weaned rabbits of both sexes were randomly divided into seven groups. This was replicated thrice with six rabbits per replicate and was assigned to 7 diets in a completely randomized arrangement. The diets involved three levels of organic chromium (0.02, 0.04 and 0.06g/kg diet) and three inorganic chromium levels (0.02, 0.04 and 0.06g/kg diet), and the treatment without chromium was the control in a completely randomized design. Feed and water were supplied to the animals’ ad libitum. Data were collected on performance and blood indices. Dietary inclusion of organic and inorganic chromium supplementation had a significant (p< 0.05) effect on all parameter measured respectively. The diet containing 0.04g/kg and 0.06g/kg organic chromium produced the superior weight gains per day and better feed conversion ratio. It was concluded and recommended that an addition of organic chromium at 0.04g/kg and 0.06g/kg can be successfully supplemented in weaned rabbit’s diets without any negative effect on their performance and their blood biochemical.

Keywords | Blood indices, Climate change, Growth performance, Inorganic chromium, Organic chromium, Weaned rabbits


Received | June 21, 2023; Accepted | October 17, 2023; Published | October 23, 2024

*Correspondence | Oghenebrorhie Obakanurhe, Department of Animal Production Dennis Osadebay University, Asaba, Delta State, Nigeria; Email: [email protected]

Citation | Irikefe-Ekeke EP, Obakanurhe O, Moemeka AM, Sorhue UG, Amata IA, Onwumelu IJ (2024). Leverage of chromium supplementation on growth and blood profiles in weaned rabbits. Adv. Anim. Vet. Sci. 12(12): 2356-2363.

DOI | https://dx.doi.org/10.17582/journal.aavs/2024/12.12.2356.2363

ISSN (Online) | 2307-8316; ISSN (Print) | 2309-3331

Copyright: 2024 by the authors. Licensee ResearchersLinks Ltd, England, UK.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).



INTRODUCTION

Chromium is the 21st most abundant mineral in the earth’s crust. Theoretically, chromium may occur in the oxidation states from -2 to +6, however, it is mostly found in the trivalent and hexavalent forms (Amata, 2013). Trivalent chromium (Cr3+) happens to be the more stable oxidation state in which chromium is found in living organisms and is considered to be a highly safe form of chromium (Amata, 2013) as trivalent chromium (Cr(III) plays a primary role in animal metabolism as a constituent of the Glucose Tolerance Factor (GTF), and as anenzymatic complex able to increase insulin activity (Monga et al., 2022). Trivalent chromium exist in organic and inorganic complexes, which exists in small insignificant quantities in grains. The principal problem of this research is that Chromium supplementation is usually not considered an essential trace mineral for our livestock, however, studies (Sun et al., 2018) have provided evidence that suggests that it has a nutritional (micronutrient) and physiological role in animal nutrition. 

Chromium supplementation has been found to reduce the negative effects of stress (Sahin et al., 2001; Shimaa et al., 2019; Hashem and Al-saadi, 2019) in rabbits, and in recent years, there has been considerable research interest in the utilization of Chromium (Cr) in animal feeds,however, its intake in feeds is relatively low because of its insignificant quantities in feedstuffs. Several authors have established its possible inclusion for optimum growth performance in both animals and man (Sahin et al., 2001Moeini et al., 2011; Rao et al., 2012; Rajalekshmi et al., 2014Shimaa et al., 2019; Hashem and Al-saadi, 2019; Van Hoeck et al., 2020) with the recommended quantity of chromium for adult human consumption been up to 200ppb of trivalent chromium (Moghanapriya et al., 2018). 

However, in rabbit nutrition, there have been no levels of inclusion recommended (NRC, 1994; Shittu et al., 2022), although in ruminants, supplementation during heat stress periods, early lactation, and during infections has shown significant results, Its administration up to 5mg/head/day at the last three weeks pre-partum and about 6mg/head/day at the first few weeks post-partum has been effective. Thus, it became necessary to investigate the role of chromium in its organic and inorganic forms in weaned rabbits with a view to its potential toxicity (Amata, 2013; Zoroddu et al., 2019), with an aim to investigate the effects of organic and inorganic chromium supplementation in the weaned rabbit’s diets.

MATERIALS AND METHODS

Experimental Location and House

The experiment was carried out in the Rabbit unit, Department of Animal Science Farm, Delta State University Asaba Campus, Delta State, which is located on longitude 60 Celsuis 450E and Latitude 60 Celsius 120 N with the average monthly temperature varying between 27.5 and 32.9o Celsius, and an average relative humidity of 60- 85% (Asaba Metrological Station, 2023). The Rabbit unit is dwarf-walled and fully netted for proper ventilationand to prevent birds and rodentsfrom coming in contact with the experimental animals. The entrance of the rabbit unit has a foot dip to ensure proper disinfection (EndoSan Disinfectant) when accessing the unit, which contains well-constructed hutches of 1m by 1m made of wooden poles to house the animals, and each hutch having a drinker and two feeders made from aluminum and secured to the walls of the hutches to avoid toppling.

Experimental Animals and Ethical Approval

This experiment was approved by the Board of Studies (BOS), Department of Animal Science 0021/27, Delta State University, Abraka Nigeria. A total of 126 composite breeds of weaned rabbits (35 days old) were used for this experiment.

Animals Management

The rabbits were treated against internal parasites using albendazole, and against external parasites using diazintol. Animals were also given treatments against coccidiosis. The animals were exposed to the experimental diets a week before data collection, to acclimate them to the diets. Feeds were weighed and offered to the animals twice a day (8:00 am and 5:00 pm) and the left-over were also weighed the next morning prior to fresh feed. The water administered were certified for consumption from the farm’s bore-hole by the Ethical Committee on Animal Welfare in the School.

Experimental Diets and Design

Using a completely randomized design, the weaned rabbits were allotted into 7 treatments with 3 replicates of 6 animals per replicate (126 animals). The control (T1) had no chromium supplementation, while the other treatments had graded levels of organic and inorganic chromium supplementation. Brewer’s dry grains (BDG) (4.381g/kg embedded chromium) and chromium chloride were used as organic and inorganic sources of Chromium, respectively.

Treatment groups consisted of chromium supplementation levels as follows: Control Group (TI) no supplementary chromium in the experimental diet, T2 contained 0.02 g/kg chromium in BDG in the experimental diet, T3 contained 0.02g/kg of supplemental chromium chloride, T4 contained 0.04g/kg of chromium in BDG, T5 contained 0.04g/kg of supplemental chromium chloride, T6 contained 0.06g/kg of chromium in BDG, and lastly, T7 contained 0.06g/kg of supplemental chromium in the diet.

The entire feeding trial lasted for four weeks. During the experiments, the animals were fed with the different experimental feeds and provided with fresh drinking water adlibitum.

Data Collection

Performance characteristics fed experimental diets: The animals were weighed once a week in the morning before feeding. Live weight changes were estimated as the difference between the initial and final body weights of the animals, feed intake, and feed conversion were analyzed according to Helen et al. (2020) and Obakanurhe et al. (2022).

 

Table 1: Compositions of dietary treatments fed the weaned rabbits.

Ingredients

T1

T2

T3

T4

T5

T6

T7

Maize (kg)

42.40

38.00

42.40

37.54

42.40

36.70

42.00

Wheat offal (kg)

28.60

29.70

28.20

26.40

28.76

24.00

29.50

Soya bean meal (kg)

15.50

14.40

15.50

14.00

15.30

13.80

15.34

Groundnut cake (kg)

9.00

8.80

9.00

8.36

9.00

7.20

9.00

Fish meal (kg)

1.50

1.50

1.50

1.50

1.50

1.50

1.50

Bonemeal (kg)

2.50

2.50

2.48

2.50

2.48

2.50

2.30

BDG (kg)

----

4.60

---

9.20

---

13.80

---

Salt(kg)

0.20

0.20

0.20

0.20

0.20

0.20

0.20

Oil(kg)

0.10

0.10

0.10

0.10

0.10

0.10

0.10

Premix(kg)

0.20

0.20

0.20

0.20

0.20

0.20

0.20

Chromium (g/kg)

-----

-----

0.02

----

0.04

----

0.06

Total

100.00

100.00

100.00

100.00

100.00

100.00

100.00

Calculated values

Crude Protein (%)

19.13

19.63

19.14

19.72

19.17

19.78

19.19

Crude fibre (%)

5.14

5.62

5.12

5.63

5.13

5.66

5.22

Ether Extract (%)

4.64

4.81

4.64

4.83

4.66

4.88

4.68

Metabolizable energy/Kcal.Kg

2598.05

2535.16

2596.01

2554.06

2599.60

2588.35

2604.17

 

Provided: Vit A:20500000iu; vit D3: 4,250,000iu; Vit E: 250,000iu; Vit K: 8000mg; Vit B1:– 20,000mg; Vit B2: 15,000mg; Vit B6: 20,0000mg; Vit B12: 15.00mcg; Vit B3: 90,000mg; Vit B5: 40.00mg; Vit B4: 4,000 mg; Vit C: 350,000mg; Vit B7: 500mcg; Choline Cloride: 600,000mg; Iron: 40,000mg; Manganese: 30,000mg; Zinc: 40,000mg; Magnesium: 60,000mcg; Copper: 4,000mg; Iodine: 2,000mg; Cobalt: 750mg; Selenium: 200mcg; Molybdenum: 100mg; Inositol: 50,000mg; Methionine; 50,000mg; Lysine: 50,000mg; Antioxidant: 125,000mg.

 

Table 2: Proximate compositions of dietary treatments fed to weaned rabbits.

Parameters

T1

T2

T3

T4

T5

T6

T7

Moisture (%)

9.81

13.44

12.34

13.01

10.34

12.34

10.23

Crude Protein (%)

17.50

17.52

17.25

18.01

17.55

17.85

17.60

Crude fibre (%)

8.58

9.06

9.66

8.87

8.92

8.76

8.56

Ether Extract (%)

5.34

5.67

4.88

4.67

4.23

4.98

4.67

Ash (%)

4.25

4.94

4.35

4.88

5.04

3.86

4.54

Nitrogen free extracts

54.52

49.37

51.52

50.56

53.92

52.21

54.40

Metabolizable energy/Kcal.Kg

2453.00

2217.81

2284.74

2256.76

2275.70

2230.35

2276.04

 

Blood indices of weaned rabbits fed experimental diets: At the end of the feeding period, at 9 weeks old, blood samples were collected from three rabbits per replicate for haematological and blood biochemical analysis (63 blood samples). The ear marginal vein was used to bleed the rabbits, the samples were divided into two lots and used for haematological and biochemical research . Each sample’s initial 2.5 ml was taken and utilized for haematological examination in a labeled, sterile, universal vial containing 1.0 mg/ml ethyldiaminetetracetic acid. A further 2.5 ml was taken from the anticoagulant-free bottle. Within three hours of collection, the blood was allowed to clot at room temperature before the serum was separated by centrifugation. Using the Beckman Coulter Ac-T10 Laboratory Haematology Blood Analyzer and the Bayer DCA 2000+ HbA1c analyzer, respectively, serum biochemistry and haematological parameters were assessed according to Onwumelu et al. (2022).

Data Analysis

According to Obakanurhe and Akpodiete (2021), all Data collected during this study were subjected to analysis of Variance (ANOVA) in a Completely Randomized Design (CRD) using GenStat (Release 4.24) statistical package (Genstat, 2014). Differences between means were separated by Duncan’s Multiple Range Test (DMRT).

RESULTS AND DISCUSSION

Proximate Compositions of Experimental Diets

As the inclusion levels (brewers dry grains and chromium chloride), increased across the experimental treatments, there were varying values of DM, CP, EE and CFof the proximate compositions of experimental diets, with treatment T3 and T4 recording the least and highest values respectively. The ash and ether extract recorded range from 3.86% – 5.04% and 4.23% – 5.67% respectively.

Performance Characteristic of Weaned Rabbit Fed Experimental Diets

The performance characteristics of rabbits on dietary treatments showed significant (p<0.05) differences in all parameters analysed in Table 3. There was an increase in the final body weight, with Treatment 6 being significantly (p<0.05) higher than treatments 2, 4, and 7, with treatments 6 and 7 recording the highest and least values respectively. For weight gain, it was observed that the final weight results recorded followed a similar trend as to the weight gain result, with treatments 2, 4 and 6 had higher mean values compared to the other treatments; this could be attributed to the organic chromium which is absorbed better than the inorganic sources (Irikefe et al., 2020; 2024). Daily feed intake results observed showed that treatments 2 and 5 recorded the highest and lowest mean values among the treatment means, however, treatment 6 showed a superior feed conversion ratio, which can be an indication that organic chromium supplementation up to 0.06 g/kg in the diet did not negatively affluence consumption, while treatments 3 and 7 which had the lowest values respectively could be an indication of the negative effect of inorganic chromium on feed intake.

Haematology and Serology Parameters of Weaned Rabbit Fed Experimental Diets

Dietary treatments had significant (p<0.05) effects on all haematological and serological parameters as shown in Table 4. The haemoglobin, RBC and WBC of weaned rabbits on treatment 6 showed significantly (p<0.05) higher values than in other treatments.

The values generated for the daily weight gains of the weaned rabbits in this study were comparable with values of 9.31 - 12.71g obtained by Evans et al. (2022), in their research on the evaluation of weaned rabbit’s growth performance and some haematological characteristics fed pawpaw peel meal-based diets in cross river rain forest zone, and also with values of 10.38- 15.18g by Ebegbulem et al. (2017),

 

Table 3: Performance characteristics of weaned rabbits fed experimental diets.

Parameters

T1

T2

T3

T4

T5

T6

T7

IWT(g)

413.3±17.63ab

415±8.6ab

406±7.26b

414±13.38ab

387±6.61c

437±3.14a

391±5.49c

FWTg)

744.6±10.12b

761±8.66b

676±4.30cd

758±6.74b

694±3.28c

814±5.72a

643±3.81d

TWG(g)

331±7.57ab

346±3.55ab

275±9.52cd

331±10.7ab

307±13.36bc

377±2.59a

251±2.69d

ADG(g)

11.82±0.20b

12.35±0.08a

9.82±0.22c

11.82±0.25b

10.96±0.82bc

13.46±0.06a

8.96±0.05d

DFI(g)

42.87±0.34bc

45.12±0.11a

43.14±0.02bc

44.28±0.58ab

41.69±0.40c

42.8±0.80bc

43.05±0.76bc

FCR

3.71±0.12b

3.65±0.07b

4.38±0.18b

3.75±0.31b

3.80±0.87b

3.17±0.15c

4.80±0.10a

 

a,b,c,d Means within the row with different superscripts are different at P < 0.05; IWT: initial weight; FWT: final weight; TWG: total weight gains; ADG: average daily gains; DFI: daily feed intake; FCR: feed conversion ratio.

 

Table 4: Haematology and serology parameters of weaned rabbits fed the experimental diets.

Parameters

T1

T2

T3

T4

T5

T6

T7

PCV(%)

36.17 ± 0.03b

36.20 ± 0.06b

35.17 ± 0.20c

36.23 ± 0.03a

34.33 ± 0.17d

36.17 ± 0.03b

34.03 ± 0.03e

HB(g/dl)

11.51 ± 0.02b

11.02 ± 0.02c

11.52 ± 0.01b

10.88 ± 0.01d

10.54 ± 0.06e

11.63 ± 0.06a

10.89 ± 0.03d

RBC(x1012/l)

5.54 ± 0.03c

5.64 ± 0.003b

5.34 ± 0.06f

5.45 ± 0.03d

5.38 ± 0.03e

6.02 ± 0.06a

5.38 ± 0.03e

WBC(x109/l)

6.13 ± 0.03d

6.25 ± 0.01b

6.18 ± 0.003c

5.85 ± 0.01g

5.95 ± 0.02f

6.84 ± 0.03a

6.10 ± 0.00e

cholesterol(g/dl)

71.84 ± 0.01b

69.51 ± 0.01d

70.17 ± 0.02cd

70.38 ± 0.05c

72.88 ± 0.28a

71.73 ± 0.06b

69.55 ± 0.05d

Total protein (g/100ml)

6.40 ± 0.01b

6.40 ± 0.01b

6.17 ± 0.02c

6.42 ± 0.03b

6.53 ± 0.06a

6.52 ± 0.03a

6.43 ± 0.03c

Creatine (mg/dl)

1.52 ± 0.03a

1.51 ± 0.03a

1.45 ± 0.01b

1.52 ± 0.01a

1.44 ± 0.01b

1.52 ± 0.03a

1.41 ± 0.03c

Urea (mmol/l)

23.8 ± 0.01a

23.25 ± 0.03ab

22.32 ± 0.27c

23.32 ± 0.34ab

22.71 ± 0.06cb

23.50 ± 0.33a

22.65 ± 0.01cb

Albumin (g/l)

40.76 ± 0.40a

38.64 ± 0.02c

37.32 ± 0.10d

38.55 ± 0.02c

37.11 ± 0.33d

39.45 ± 0.05b

36.53 ±0.01e

Glucose (g/dl)

83.58 ± 0.31a

82.67 ± 0.10a

81.40 ± 0.01b

79.04 ± 0.54c

75.85 ± 0.67d

82.83± 0.00a

76.51 ± 0.05d

 

a-b-c-d Means within the row with different superscripts are different at P < 0.05; PCV: packed cell volume; HB:haemoglobin; RBC: red blood count; WBC: white blood count.

 

on the growth performances and carcass characteristics of weaned rabbits fed Alchornea cordielia leaf meal but was higher than the values 4.48 - 6.32g obtained by Wafer et al. (2018) and Adedeji et al. (2006), on their research on performance and blood indices of weaned rabbits fed boiled castor seed cake diets. This is a clear indication of the chromium playing a role of a metabolic modulator to balance metabolism (Guo et al., 2020). It is noted that the values of average daily weight gains from this research were different from the values (17.8 – 21.3g) obtained by Mervat Ghazal et al. (2013), in their research on growing rabbit’s productive and physiological responses to dietary organic chromium addition. Also, similar values (32.91g -33.44g) were obtained in a separate study by Amer et al. (2019) on the effect of dietary chromium supplementation on performance, some blood indices and tissue chromium contents of rabbits. These observed values could be attributed to the time frame during which these researches were carried out (16 weeks) without distinction between weaner phase and grower phase which was in contrast to this study that specified the weaner phase as the first four weeks, afterwards the grower phase.

Based on average feed intake, values from this research (41.69 – 45.12g) were comparable to several authors (Oyawoye and Ogunkunle, 1998; Adedeji et al., 2006; Onyekwere et al., 2010; Ozung et al., 2012) but lower than the values obtained by Ebegbulem et al. (2017). Feed efficiency performance were comparable for the control, treatments 2, 4, 5, and 6 , which was different from the values reported by several authors (Adedeji et al. 2006; Ozung et al. 2012; Amer et al. 2019) were lower. The results for feed intake and efficiency in this research were similar to results reported by Van Hoeck et al. (2020), who used organic chromium in the form of chromium propionate in the broiler finishing phase. These results from this experiment however, differ from the reports of Ibrahim et al. (2022) and Şahin et al. (2001), who observed that chromium-yeast supplementation did not affect daily weight gain, and feed efficiency during the rabbit fattening period. Although, their results are not clear, since most researchers agree that chromium supplementation during periods of higher stress has a significant effect on weight gain. These discrepancies could be attributed to an increase in the glucose conversion to acetyl-CoA brought on by the addition of organic chromium (Moffett et al., 2020). Acetyl-CoA is essential for biological reactions and is employed in many of them (Moffett et al., 2020). The changes observed in the final body weight across treatments with organic chromium could be as a result of the influence of organic chromium addition to the biological activity of insulin by improving the insulin-sensitive cell receptors or binding activity (Levina and Lay, 2019). This could account for the significantly better final body weight of weaned rabbits during the entire fattening period. Overall, experimental animals on the organic chromium sources compared favourably to the control. This indicates the place of organic over inorganic. However, the values recorded in this experiment were still within the normal range. The values recorded from adding organic and inorganic chromium in this study were in agreement with a separate study involving broiler-fed chelated chromium (Ibrahim et al., 2022), which confirms that chelated chromium inclusion could enhance immune system activity.

In contrast to these present results, Adebiyi and Makanjuola (2011) and Sarai et al. (2022), recorded no significant (P≤0.05) differences in the Hb, WBCs, and RBCs for broiler chickens fed with 0.25 mg/kg chromium chloride. In general, chromium inclusion increases the total RBCs, which also revealed increase in theHb values (Shah et al., 2020), this is linked to the positive interaction between RBCs and Hb. The inclusion of chromium increases the Haemoglobin values (within a normal range) which aligned with its effects on haematological parameters (Feldman et al., 2000). Cholesterol levels showed significant differences (p<0.05). The level increases as the chromium level increases (organic and inorganic). This trend was also observed by Anthony and Ashawe (2014), in their experiment on the effect of Moringa oleifera leaf meal (MOLM) on the haematological parameters and the cholesterol level of rabbits, which also agrees with the work of Anthony and Ashawe, (2014).

All values for cholesterol in this experiment fell within the range reported by Medirabbits (2011), it can therefore be concluded that the supplementation of these levels of chromium can conveniently be used in feeding weaned rabbits without any detrimental effects on cholesterol levels. This study’s results therefore indicate that 0.02g/kg, 0.04g/kg and 0.06g/kg of organic chromium and 0.04g/kg of inorganic chromium supplementation did not influence the protein, urea, and cholesterol concentrations of rabbits, which also agrees with results by other authors using other animals (Saeed et al., 2017: Lashkari et al., 2018). Lashkari et al. (2018) reported that serum total protein, urea, and triglycerides were not affected by supplementation of chromium, this is in contrast with this research and several other studies which have documented that chromium supplementation decreased serum triglycerides (Lashkari et al., 2018; Staniek et al., 2020). Also, in a seperate study on cow, chromium supplementations in the diets of calves did not affect the serum cholesterol, protein, urea, and triglycerides (Ansari et al., 2022).

CONCLUSIONS AND RECOMMENDATIONS

This study revealed that while the different levels of chromium inclusion significantly influenced the performance characteristics and blood indices of weaned rabbits studied, organic chromium inclusion especially at 0.04g/kg (T4) and 0.06g/kg (T6) had a greater influence on the various parameters studied. It is recommended that the inclusion levels in this study were very tolerable without no detrimental effects on the rabbit.

ACKNOWLEDGMENTS

We acknowledge the entire laboratory staffs for their time and assistance.

NOVELTY STATEMENT

To our knowledge, this study is the first to evaluate the presence of chromium in brewers dried grains in Nigeria, also the influence and recommendation of 0.04- 0.06g/kg of organic chromium for weaned rabbits are published for the first time by this study. 

AUTHOR’S CONTRIBUTIONS

Efe Peterson Irikefe- Ekeke assisted in conceptualization, methodology, writing original draft, Efe Peterson Irikefe- Ekeke and Oghenebrorhie Obakanurhe assisted in data collection, Data Analysis and designing of the manuscript. Ifo Alex Amata supervised and evaluated the manuscript content. UfuomaGodstimeSorhue, Adimabua Mike Moemeka and Ifeoma Jane Onwumelu proof read and drafted the manuscript.

Funding

The research received no external funding.

Conflicts of Interest

The authors declare no conflict of interest.

REFERENCES

Adebiyi AO, Makanjuola BA (2011). Effect of inorganic chromium supplementation on performance, meat quality, residual chromium and mineral composition of heat stressed broiler birds. International journal of agriscience vol 1(7): 273-380.

Adedeji JA, Apata DF, Aderinola OA, Rafiu TA, Amao SR (2006). Performance and haematological/serum characteristics of rabbits fed boiled castor seed cake based diet. World J. Zool., 1(2): 91- 93.

Amata IA (2013). Chromium in livestock Nutrition; A Review, Global Adv. Res. J. Agric. Sci., 12: 289-306.

Amer SA, Omar AE, Abd El-Hack ME (2019). Effects of selenium-and chromium-enriched diets on growth performance, lipid profile, and mineral concentration in different tissues of growing rabbits. Biol. Trace Elem. Res., 187, 92-99. https://doi.org/10.1007/s12011-018-1356-4

Ansari M, Kargar S, Eslami MA, Falahati R, Albenzio M, Caroprese M, Kanani M (2022). Potential benefits of early-life supplementation of liquid feed with fennel (Foeniculumvulgare) seeds or oregano (Origanumvulgare) leaves on growth, health, and blood metabolites in Holstein dairy calves. J. Dairy Sci., 105(8): 6639-6653. https://doi.org/10.3168/jds.2022-21776

Anthony VP, Ashawe D (2014). The effect of Moringaoleifera leaf meal (molm) on the hematological parameters and the cholesterol level of rabbits. Am. J. Biol. Chem. Pharm. Sci., 2(3): 1-6.

Asaba Metrological Station (2023). Nigerian Meteorological Agency, Asaba Station.

Ebegbulem VN, Dauda A, Udoudo M (2017). Growth performance and carcass characteristics of weaned rabbits fed Alchornea cordieolia leaf meal. Acad. J. life Sci., 3 (12): 94-97.

Evans EI, Sam IM, Ukpanah UA (2022). Replacement value of cassava peel meal for maize on the reproductive potential of rabbit does in Nigerian tropical region. Niger. J. Anim. Prod., 49(1): 313-321.

Feldman, L. J., Aguirre, L., Ziol, M., Bridou, J. P., Nevo, N., Michel, J. B., Steg, P. G. (2000). Interleukin-10 inhibits intimal hyperplasia after angioplasty or stent implantation in hypercholesterolemic rabbits. Circulation, 101(8), 908-916.

Genstats (2014). VSN international, Hemel Hermpstead, UK. Webpage: Genstat.co.uk

Guo WL, Chen M, Pan WL, Zhang Q, Xu JX, Lin Y, Lv C, Lv XC (2020). Hypoglycemic and hypolipidemic mechanism of organic chromium derived from chelation of Grifolafrondosa polysaccharide-chromium (III) and its modulation of intestinal microflora in high fat-diet and STZ-induced diabetic mice. Int. J. Biol. Macro-Mol., 145: 1208-1218. https://doi.org/10.1016/j.ijbiomac.2019.09.206

Hashem SH, Al-Saadi MJ (2019). Chromium picolinate effects in diet of male rabbits exposed to heat stress on some productive traits and blood serum. The Iraqi J. Agric. Sci. Baghdad,  50(3):1100-1108. https://doi.org/10.36103/ijas.v50i4.755

Helen ON, Job AO, Oghenebrorhie O (2020). Antibiotic and antihelminthic effects of garlic bulbs (Allium sativum) and ginger rhizomes (Zingiber officinale) as growth promoters on broiler chickens in the tropics. Adv. Anim. Vet. Sci., 8 (10): 1019-1027. https://doi.org/10.17582/journal.aavs/2020/8.10.1019.1027

Ibrahim H, Gajere J, Zaharadeen A, Abdulmalik S (2022). Effect Of Dietary Supplementation of Baker’s Yeast (Saccharomyces Cerevisiae) On Growth Performance And Carcass Characteristics Of Weaner Rabbits (Oryctolagus Cunniculus). Fudma J. Agric. Agric. Technol., 8(1): 64-69. https://doi.org/10.33003/jaat.2022.0801.021

Irikefe-ekeke, E. P., Okagbare, G. O., Sorhue, G. U. (2020). Comparative performance of West African Dwarf goats fed Alchornea cordifolia and Gliricidia sepium supplemented with Pennisetum purpureum. Nigerian Journal of Animal Science, 22(3), 325-330.

Irikefe-ekeke, E. P., Obakanurhe, O., Bratte, L. I., Adimabua, M. M., Ade, S. O., Onwumelu, J. (2024). Growth performance, nutrient digestibility and carcass characteristic of growing rabbits fed diets supplemented with organic and inorganic chromium. Nigerian Journal of Animal Production, 50(3), 1-10.

Lashkari S, Habibian M, Jensen SK (2018). A review on the role of chromium supplementation in ruminant nutrition—effects on productive performance, blood metabolites, antioxidant status, and immunocompetence. Biol. Trace Elem. Res., 186: 305-321. https://doi.org/10.1007/s12011-018-1310-5

Levina A, Lay PA (2019). Redox chemistry and biological activities of chromium (III) complexes. Nutr. Biochem. Chromium (III) (281-321). Elsevier. https://doi.org/10.1016/B978-0-444-64121-2.00009-X

Makanjuola BA, Adebiyi OA (2012). Effect of Inorganic Chromium Supplementation on the Intestinal Mucosa Development of Heat-Stressed Broilers. Global Journal of Science Frontier Research. 12 (5) Version 1.0. Double Blind Peer Reviewed International Research Journal. Global J. Inc. (USA).

Medirabbit., 2011. Complete blood count and biochemical reference values in rabbits. Retrieved from www.medirabbit.com, 22nd July, 2016, 12:30 pm

Mervat N, Ghazal H, Hoda MA, Shabaan SZ, Gad-Alla EM, Abdel-Kafy K, El-kholy H (2013). Productive and Physiological Responses of Growing Rabbits to Dietary Organic Chromium Addition. Proc. 4th Sci. Conf. Anim. Prod. Res. Inst., 384-398

Moeini MM, Bahrami A, Ghazi S, Targhibi MR (2011). The effect of different levels of organic and inorganic chromium supplementation on production performance, carcass traits and some blood parameters of broiler chicken under heat stress condition. Biol. Trace Elem. Res., 144: 715-724. https://doi.org/10.1007/s12011-011-9116-8

Moffett JR, Puthillathu N, Vengilote R, Jaworski DM, Namboodiri AM (2020). Acetate revisited: A key biomolecule at the nexus of metabolism, epigenetics and oncogenesis—Part 1: Acetyl-CoA, acetogenesis and acyl-CoA short-chain synthetases. Front. Physiol., 11: 580167. https://doi.org/10.3389/fphys.2020.580167

Moghanapriya GP, Veeramani P, Pasupathi K (2018). Concentration of chromium in egg yolk and meat of Japanese quails in response to dietary chromium supplementation. Indian J. Anim. Hlth, 57(1): 93-98. https://doi.org/10.36062/ijah.57.1.2018.93-98

Monga A, Fulke AB, Dasgupta D (2022). Recent developments in essentiality of trivalent chromium and toxicity of hexavalent chromium: implications on human health and remediation strategies. J. Hazard. Mater. Adv., 7: 100113. https://doi.org/10.1016/j.hazadv.2022.100113

Mowat DD (1994). Organic chromium. A new nutrient for stressed animals. In: Biotechnology in the Feed Industry (Lyons, T.P. and Jacques, K.A.eds), University Press, Nottingham, UK., 275-282.

National Research Council (NRC) (1994). Nutrient requirement of poultry. 9th rev. edition. National Academy press, Washington, DC.

Obakanurhe O, Akpodiete OJ (2021). Performance characteristics of broiler chickens fed sweet potato (Ipomoea batatas) leaf in the tropics. Livestock Res. Rural Dev., 33 (1).

Obakanurhe O, Onwumelu IJ, Irikefe-Ekeke EP, Adimabua MM, Iwegbu A, Inweh AD (2022). Influence of Varied Dietary Inclusion of Cassava Root Meal on Performance Characteristics of Broiler Chickens in the Starter Phase. Proc. 56th Ann. Confer. Agric. Soc. Niger., 1145-1149.

Onwumelu IJ, Obakanurhe O, Iwegbu A, Moemeka AM, Inweh DA, Irikefe-Ekeke EP (2022). Haematological and Serum Biochemical Parameters of Finisher Broilers fed Vernonia Amygdalina and Ocimum Gratissimum Meals. Proc. 56th Ann. Confer. Agric. Soc. Niger., 1155-1159.

Onyekwere, M. U., Olabode, A. D., Okechukwu, S. O and Iheukwmere F. C., (2010).Effects of feeding boiled bambara nut (Voandeia subterranean) waste on performance haematology and serum biochemistry of weaned rabbits. Proceedings 33rd conference of Nigerian Society for Animal production, 14-17th march, 2010, University of Ibadan. pp 203

Oyawoye EO, Ogunkunle M (1998). Physiological biochemical effects of reaw jack beans on broilers. Proc. Of 23rd Niger. Soc. Anim. Prod., 141-142.

Ozung PO, Ubua JA, Anya MI, Nsa, EE, Ebegbulem VN, Etim SE (2012). Evaluation of Growth Performance and some Haematological Characteristics of weaned rabbits fed pawpaw peel meal based diets in Cross River Rainforest Zone. Int. J. Sci. Nat. (IJSN). 3 (4): 784-787.

Rajalekshmi MC. Sugumar H, Chirakkal S, Ramarao V (2014). Influence of chromium propionate on the carcass characteristics and immune response of commercial broiler birds under normal rearing conditions. Poult. Sci., 93: 574-580. https://doi.org/10.3382/ps.2013-03373

Rao SVR, Raju, MVLN, Panda AK, Poonam NS, Murthy OK, Sunder GS (2012). Effect of dietary supplementation of organic chromium on performance, carcass traits, oxidative parameters and immune responses in commercial broiler chickens. Biol. Trace Elem. Res., 147: 135-141. https://doi.org/10.1007/s12011-011-9314-4

Saeed AA, Sandhu MA, Khilji MS, Yousaf MS, Rehman HU, Tanvir ZI, Ahmad T (2017). Effects of dietary chromium supplementation on muscle and bone mineral interaction in broiler chicken. J. Trace Elem. Med. Biol., 42: 25-29. https://doi.org/10.1016/j.jtemb.2017.03.007

Sahin K, Kucuk O, Sahin N, Ozbey O (2001). Effects of dietary chromium picolinate supplementation on egg production, egg quality and serum concentrations of insulin, corticosterone and some metabolites of Japanese quails. Nutr. Res., 21: 1315-1320. https://doi.org/10.1016/S0271-5317(01)00330-X

Sarai FJS, Shakouri MD, Seidavi A (2022). Effect of Chromium Picolinate, Alone or in Combination with Vitamin C or Formic Acid on Growth, Carcass Traits, Immune and Blood Parameters of Broilers under Heat Stress.

Shah N, Khisroon M, Shah SSA (2020). Assessment of copper, chromium, and lead toxicity in fish (CtenopharyngodonidellaValenciennes, 1844) through hematological biomarkers. Environ. Sci. Pollut. Res., 27(26): 33259-33269. https://doi.org/10.1007/s11356-020-09598-z

Shimaa A, Amer A, Onar E, Mohamed E, Abd El-Hack E (2019). Effects of Selenium- and Chromium-Enriched Diets on Growth Performance, Lipid Profile, and Mineral Concentration in Different Tissues of Growing Rabbits. Biol. Trace Elem. Res., 187: 92-99. https://doi.org/10.1007/s12011-018-1356-4

Shittu MT, Alagbe JO, Ojebiyi OO, Ojediran TK, Rafiu TA (2022). Growth performance and haematological and serum biochemical parameters of broiler chickens given varied concentrations of Polyalthialongifolia leaf extract in place of conventional antibiotics. Anim. Sci. Genet., 18(2). https://doi.org/10.5604/01.3001.0015.9185

Staniek HZ, Król E, Wójciak RW (2020). The interactive effect of high doses of chromium (III) and different Iron (III) Levels on the carbohydrate status, lipid profile, and selected biochemical parameters in female wistar rats. Nutrients, 12(10): 3070. https://doi.org/10.3390/nu12103070

Sun GY, Simonyi A, Fritsche KL, Chuang DY, Hannink M, Gu Z, Beversdorf DQ (2018). Docosahexaenoic acid (DHA): An essential nutrient and a nutraceutical for brain health and diseases. Prostaglandins, Leukot. Essent. Fatty Acids, 136: 3-13. https://doi.org/10.1016/j.plefa.2017.03.006

Van Hoeck V, Sonawane M, Sanchez ALG, Van Dosselaer I, Buyens C, Morisset D (2020). Chromium propionate improves performance and carcass traits in broilers. Anim. Nutr., 6(4): 480-87. https://doi.org/10.1016/j.aninu.2020.03.005

Wafar RJ, Yakubu B, Yusuf HB, Antyev M (2018). Effect of feeding raw and differently processed kapok (Ceibapentandra) seed meal on the growth performance and carcass characteristics of weaner rabbits. Niger. J. Anim. Sci., 20(2): 258-270.

Zoroddu MA, Aaseth J, Crisponi G, Medici S, Peana M, Nurchi VM (2019). The essential metals for humans: a brief overview. J. Inorg. Biochem., 195: 120-129. https://doi.org/10.1016/j.jinorgbio.2019.03.013

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