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Expression profile of Toll-like Receptors Pathway Genes in Chicken Erythrocytes Infected with Mycoplasma synoviae

Expression profile of Toll-like Receptors Pathway Genes in Chicken Erythrocytes Infected with Mycoplasma synoviae

Xin-yu Han1,2, Afrasyab Khan1,2, Ali Raza Jahejo1,2, Qian-qing Cheng1,2, Meng-li Qiao1,2, Raza Ali Mangi1,2, Muhammad Farhan Qadir1,2, Ding Zhang1,2, Ying Wang1,2, Yu-hai Bi1,3, Rui-wen Fan1,2 and Wen-xia Tian1,2

1College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China
2Shanxi Key Laboratory of protein structure determination, Shanxi Academy of Advanced Research and Innovation, Taiyuan 030032, China
3CAS Key Laboratory of Pathogenic Microbiology and immunology, Collaborative Innovation    Center of Infectious Diseases, Institute of Microbiology, Center for Influenza Research and Early-Warning (CASCIRE), Chinese Academy of Science, Beijing 100101, China 
 
Xin-Yu Han and Afrasyab Khan have contributed equally to this study.
 
* Corresponding author: wenxiatian@126.com

Fig. 1.

The transmission electron micrograph (TEM) revealed intearction between erythrocytes with M. synoviae.

Fig. 2.

The expression pattern toll-like receptors pathway genes in chicken erythrocytes on 0, 2, 6, and 10 h. This experimental study group was as follows: the control group (Con, erythrocytes treated with PBS, n = 3) and the experiment group (Exp, erythrocytes infected with M. synoviae, n = 3). Expression levels of TLR pathway genes were relatively calculated to that of 18S rRNA the housekeeping genes via qRT-PCR. SEM (standard error of mean) represented, by error bars. Bars with asterisks indicate a significant (*P < 0.05, **P < 0.01, ***P ≤ 0.001) Up or downregulated relative mRNA expression when compared to uninfected control group.

Pakistan Journal of Zoology

February

Pakistan J. Zool., Vol. 56, Iss. 1, pp. 01-501

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