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Expression Pattern of Apoptotic Genes During Different Stages of Corpus Luteum Development in Egyptian Buffaloes

Expression Pattern of Apoptotic Genes During Different Stages of Corpus Luteum Development in Egyptian Buffaloes

Samaa M Galal1, Sally Ibrahim2, Karima Mahmoud2, Ola Adel1, Aya A. Shokry3, El-Belely MS1, Ismail Sayed Taha1* 

1Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza square, 12211, Egypt; 2Department of Animal Reproduction and AI, Veterinary Research Institute, National Research Centre, Dokki, 12622, Egypt; 3Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza square, 12211, Egypt.

*Correspondence | Ismail Sayed Taha, Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza square, 12211, Egypt; Email: Sayedtahaismail52@cu.edu.eg  

Figure 1

Expression patterns of TNFα, BAX, CASP3, FASLG, AGTR2 and NOS2 mRNAs in different stages of corpus luteum in Egyptian buffaloes. Bars are presented as mean±SEM. a,b,cStatistical differences between different stages of CL (statistical significant at P<0.001). 

Figure 2

Concentrations of NO in CL homogenate during early, mid and late stages of CL. Bars are presented as mean±SEM. a,b,cStatistical differences between different stages of CL (statistical significant at P<0.001). 

Figure 3

Histological evaluation of different stages of CL (early, mid and late stages). a) Photomicrograph of early stage of corpus hemorrhagicum, showing blood filled cavities (arrow) (H&E). b) Photomicrograph of mid stage of corpus luteum, showing complete luteinization of granulosa cells with existence of connective tissue septa (arrow) (H&E). c) Photomicrograph of late stage of corpus luteum, showing development of fibrotic masses (arrow) (H&E). 

Figure 4

Estimation of progesterone levels during different stages of CL. Bars are presented as mean±SEM. a,b,cStatistical differences between different stages of CL (statistical significant at P<0.001). 

Advances in Animal and Veterinary Sciences

May

Vol. 12, Iss. 5, pp. 802-993

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