Exposure of Zebrafish (Danio rerio) to Titanium Dioxide Nanoparticle Causes Paraptosis: Evaluation of Ovarian Follicle Ultrastructure
Exposure of Zebrafish (Danio rerio) to Titanium Dioxide Nanoparticle Causes Paraptosis: Evaluation of Ovarian Follicle Ultrastructure
Cansu Akbulut1,*, Tuğba Kotil2, Burcu Öztürk1 and Nazan Deniz Yön1
Hıstological and electron micrographic structure of ovary of control group; a,b,c, Electron micrograph of follicles; d,e,f, light micrograph of follicules. Fe, follicular epithelium. Mi, microvilli; ZR, zona radiata; O, ooplasm; M, mitochondrium; G, golgi; RER, rough endoplasmic reticulum; Po, primary oocyte; Ca, cortical alveoli; N, nucleus; No, nucleolus; CoC, cortical alveolus stage; Vo, vitellogenic oocyte; Om, mature oocyte; Yg, yolk granule. Magnification: d, x100; e, x20; f, x40.
Hıstological and electron micrographic structure of ovary of 1 mg/L TiO2 exposed group; a, degeneration at microvili structure and mitochondrial deterioration (star) at follicular epithelium cells; b, cytoplasmic vacuolization and unification (asterix) of cortical alveoli; c, mitochondrial deterioration (star); d, mitochondrial swelling (square) and loss of organization of cristae (arrow) at mitochondrium; e, cytoplasmic vacuolization (black arrow) at primary oocytes; f, loss of nucleolus organization at primary oocyte; g, severe cytoplasmic vacuolization and degeneration at cortical alveoli structure; h, Eosinophilic granules accumulation at cortical alveolus stage ZR, zona radiata; Mi, microvilli; Fe, follicular epitheium; T, teca cells; M, mitochondrium,;N, nucleus; No, nucleolus; Ca, cortical alveoli; Eg, eosinophilic granule. Magnification: e, f,g, x100; h x40.
Lıght microscopical histological and electron micrographic strucure of ovary of 4 mg/L TiO2 exposed group. A, cytoplasmic vacuolization (red arrow) at follicular epithelium and ooplasm; b, multilamellar vesicle and cytoplasmic vacuolization (star); c, mitotic catastrophe; d, altered mitochondrium morphology and loss of cristae organization; e, cytoplasmic vacuolization (star) and multilamellar vesicle; f, dispersion at ooplasm; g, degenerated vitellogenic oocyte; h, unification of cortical alveoli (asterix). ZR, zona radiata; Fe, follicular epithelium; M, mitochondrium; ML, multilamellar vesicle; RER, rough endoplasmic reticulum; Ve, vitelline envelope; Vo, vitellogenic oocyte; N, nucleus; CoC, cortical alveolus stage; Om, mature oocyte. Magnification: g, h, x20.
Lıght microscopic and electron micrographic structure of ovary of 2 mg/L TiO2 exposed group. A, chromatin condensation (red arrow) and cytoplasmic vacuolization (blue arrow); b, opening between zona radiata and follicular epithelium and degeneration at microvili structure; c, mitochondrial swelling (red arrow) and loss of organization of cristae (circle); d, loss of organization of cristae (circle); e, vacuolization under zona radiata (blue arrows); f, cytoplasmic vacuolization; g, unification of cortical alveoli (asterix); h, shrinking of mature oocyte. ZR, zona radiata; Fe, follicular epithelium; Mi, microvilli; M, mitochondria; G, golgi; RER, rough endoplasmic reticulum; CoC, cortical alveolus stage; Vo, vitellogenic oocyte; Om, mature oocyte. Magnification: e, f, g) x40, h) 20 x.
