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Evolutionary Divergence of Signal Transducer and Activator of Transcription 5A (STAT5A) Gene in Riverine Buffalo

Evolutionary Divergence of Signal Transducer and Activator of Transcription 5A (STAT5A) Gene in Riverine Buffalo

Muhammad Naveed1, Asif Nadeem1,2*, Maryam Javed1, Muhammad Fahad Bhutta3 and Ruqayya Bint Khalid1

1Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan
2Department of Biotechnology, Faculty of Science and Technology, Virtual University of Pakistan
3Semen Production Unit, Qadirabad, Sahiwal

*      Corresponding author: asif.nadeem@vu.edu.pk

Fig. 1.

PCR amplification products of one sample with all primers. Lane L, 1 kb Plus ladder; Lane 1, PCR product of primer 1 (381bp); Lane 2, PCR product of primer 2 (883bp); Lane 3, PCR product of primer 3 (390bp); Lane 4, PCR product of primer 4 (331bp); Lane 5, PCR product of primer 5 (731bp); Lane 6, PCR product of primer 6 (847bp); Lane 7, PCR product of primer 7 (446bp); Lane 8, PCR product of primer 8 (837bp); Lane 9, PCR product of primer 9 (456bp); Lane 10, PCR product of primer 10 (500bp); Lane 11, PCR product of primer 11 (589bp); Lane 12, PCR product of primer 12 (471bp); Lane 13, negative control (H2O).

Fig. 2.

Change in structure of STAT5A protein due to change in amino acid (Serine to Asparagine).

Fig. 3.

Phylogenetic tree of STAT5A gene.

Pakistan Journal of Zoology

June

Vol. 53, Iss. 3, Pages 801-1200

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