Estrus Detection in Synchronized Dairy Buffaloes Based on Behavioral Variables for the Assessment of NEDAP Leg and Neck Monitoring Devices
Estrus Detection in Synchronized Dairy Buffaloes Based on Behavioral Variables for the Assessment of NEDAP Leg and Neck Monitoring Devices
Ray Adil Quddus1, Nisar Ahmad1*, Jalees Ahmad Bhatti1, Anjum Khaleeq2 and Asfand Yar Khan3
Experimental design to assess the NEDAP leg and neck logger tag variables for the estrus detection in dairy buffaloes. At day -35 transrectal ultrasonography (US) was done to determine the presence of CL on ovaries and verification of cyclicity at the start of experiment. First PGF2α injection was administered at the day -34 and then 11 days apart 2nd PGF2α injection at the day of -23 was administered and after PGF2α injection US was performed at 12-h intervals until ovulation was detected. The NEDAP leg and neck tag was attached at the day of -5 relation to day of estrus (day 0). From the day -3 to estrus onset (day 0) daily US was performed until ovulation and new CL was detected on ovaries. Insemination was done on the basis of AM: AM or PM: PM rule. Conception was determined at the day of +45 through ultrasonography.
Mean±SE of the automated monitoring system behaviors for Days -3 to +3 relative to estrus (Day 0). (A) mean for feeding behaviors relative estrus; (B) mean for rumination behaviors relative to estrus; (C) mean for lying behaviors relative estrus and (D) mean for standing behaviors relative to estrus, a-c different superscripts letters show significance (P<0.01) between days.
Comparison of standing (full black bar) and lying (empty bar) bouts at 3 days before estrus (Day-3) and day of estrus (Day-0) in buffaloes.
