Detection of ESBL Encoding Gene and the Virulence Factors of Klebsiella pneumoniae from Dairy Cattle Farms in South Sulawesi
Nurhashunatil Mar’ah1, Safika Safika2*, Agustin Indrawati2
1Postgraduate Student of Medical Microbiology, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, 16680, Indonesia; 2Division of Medical Microbiology, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, 16680, Indonesia.
*Correspondence | Safika Safika, Division of Medical Microbiology, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, 16680, Indonesia; Email:
[email protected]
Figure 1:
K. pneumoniae bacteria shown mucoid, pink and convex colonies in MAC media. Arrow: single colonies of K. pneumoniae.
Figure 2:
Electrophoresis results on molecular confirmation of K. pneumoniae (1090 bp). M: Marker; ATCC: positive control; 1-11: positive isolates of K. pneumoniae.
Figure 3:
Detection of amplicon blaTEM gene (516 bp). M: 100 bp DNA Marker. Isolates 1-5,9,10,12 were positives for blaTEM gene.
Figure 4:
Detection of amplicon blaSHV gene (768 bp). M: 100 bp DNA Marker. Isolates 4,5, and 12 were positives for blaSHV genes.
Figure 6:
Detection of amplicon mrkD gene (240 bp). M: 100 bp DNA Marker. Isolates 1-12 were positives for mrkD gene.
Figure 7:
Detection of amplicon entB gene (371 bp). M: 100 bp DNA Marker. Isolates 1,3,4,6-11 were positives for entB gene.
Figure 8:
Detection of amplicon wabG gene (683 bp). M: 100 bp DNA Marker. Isolates 1, 3-12 were positives for wabG gene.
Figure 5:
Detection of amplicon blaCTX-M gene (551 bp). M: 100 bp DNA Marker. Isolate 4 were positive for blaCTX-M gene.