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Detection of ESBL Encoding Gene and the Virulence Factors of Klebsiella pneumoniae from Dairy Cattle Farms in South Sulawesi

Detection of ESBL Encoding Gene and the Virulence Factors of Klebsiella pneumoniae from Dairy Cattle Farms in South Sulawesi

Nurhashunatil Mar’ah1, Safika Safika2*, Agustin Indrawati2

1Postgraduate Student of Medical Microbiology, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, 16680, Indonesia; 2Division of Medical Microbiology, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, 16680, Indonesia.

 
*Correspondence | Safika Safika, Division of Medical Microbiology, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, 16680, Indonesia; Email: safika@apps.ipb.ac.id

Figure 1:
K. pneumoniae bacteria shown mucoid, pink and convex colonies in MAC media. Arrow: single colonies of K. pneumoniae.
Figure 2:
Electrophoresis results on molecular confirmation of K. pneumoniae (1090 bp). M: Marker; ATCC: positive control; 1-11: positive isolates of K. pneumoniae.
Figure 3:

Detection of amplicon blaTEM gene (516 bp). M: 100 bp DNA Marker. Isolates 1-5,9,10,12 were positives for blaTEM gene.

Figure 4:

Detection of amplicon blaSHV gene (768 bp). M: 100 bp DNA Marker. Isolates 4,5, and 12 were positives for blaSHV genes.

Figure 6:

Detection of amplicon mrkD gene (240 bp). M: 100 bp DNA Marker.  Isolates 1-12 were positives for mrkD gene.

Figure 7:

Detection of amplicon entB gene (371 bp). M: 100 bp DNA Marker. Isolates 1,3,4,6-11 were positives for entB gene.

Figure 8:

Detection of amplicon wabG gene (683 bp). M: 100 bp DNA Marker. Isolates 1, 3-12 were positives for wabG gene.

Figure 5:

Detection of amplicon blaCTX-M gene (551 bp). M: 100 bp DNA Marker. Isolate 4 were positive for blaCTX-M gene.

Advances in Animal and Veterinary Sciences

May

Vol. 12, Iss. 5, pp. 802-993

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