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Cardioprotective Effect of Naringenin against Myocardial Ischemia-Reperfusion Injury via Alteration of Apoptotic Signaling Pathway

Cardioprotective Effect of Naringenin against Myocardial Ischemia-Reperfusion Injury via Alteration of Apoptotic Signaling Pathway

Tao Ren1, Guiqiu Cao2, Xiao Han3, Feng Tan1, Qiaoli Chen4, Shicheng Yang5 and Haiyan Zhang6*

1Department of Third Cardiology, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang, 832008, China
2Department of Cardiology, Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, 830011, China
3Department of Cardiology,General Hospital of Fuming of Liaoning Health Industry Group, Fushun, Liaoning, 113000, China
4Department of Cardiology, First People’s Hospital of Wuyi County, Jinhua, Zhejiang, 321200, China
5Department of Cardiology, Tianjin Chest Hospital, Tianjin, 300222, China
6Department of Cardiology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, 210011, China
 
Tao Ren and Guiqiu Cao are co-first authors and contributed equally to this work.
 
* Corresponding author: ysxm53460@sina.com

Fig. 1.

Effect of naringenin on the cell viability by H/R in H9c2 cardiomyocytes. A) H9c2 cells were treated with various concentration of naringenin for 24 h and MTT assay was used for the estimation of cell viability. B) H9c2 cells were treated with for 12 h with naringenin and then exposed to 6h of hypoxia. The data presented as means±SD from three independent experiments.

Fig. 2.

Effect of naringenin on the LDH release by H/R in H9c2 cardiomyocytes. The data presented as means±SD from three independent experiments. ##P < 0.01 versus control group; *P < 0.05, **P < 0.01 versus H/R group.

Fig. 3.

Effect of naringenin on antioxidant parameter of H/R in H9c2 cardiomyocytes cells. A) CAT, B) GSH-Px, C) SOD and D) MDA. The data presented as means±SD from three independent experiments. ##P < 0.01 versus control group; *P < 0.05, **P < 0.01 versus H/R group.

Fig. 4.

Effect of naringenin on ROS concentration of H/R in H9c2 cardiomyocytes cells. The data presented as means±SD from three independent experiments. ##P < 0.01 versus control group; *P < 0.05, **P < 0.01 versus H/R group.

Fig. 5.

Effect of naringenin on the biochemical parameter of I/R induced mice. A) CK-MB, B) LDH and C) AST. Dennett’s test was used for statistically significance *P<0.05, **P<0.01 and ***P<0.001.

Fig. 6.

Effect of naringenin on the antioxidant parameters of I/R induced mice. A) SOD, B) CAT and C) MDA. Dennett’s test was used for statistically significance *P<0.05, **P<0.01 and ***P<0.001.

Fig. 7.

Effect of naringenin on the lipid parameters of I/R induced mice. A) Cholesterol, B) High density lipoprotein and C) Low density lipoprotein. Dennett’s test was used for statistically significance *P<0.05, **P<0.01 and ***P<0.001.

Fig. 8.

Effect of naringenin on the Na, Ca and K level of I/R induced mice. A) Na, B) Ca and C) K. Dennett’s test was used for statistically significance *P<0.05, **P<0.01 and ***P<0.001.

Fig. 9.

Effect of naringenin on the membrane-bound enzymes of I/R induced mice. A) Mg++ ATPase, B) Na+K+ ATPase and C) Ca++ ATPase. Dennett’s test was used for statistically significance *P<0.05, **P<0.01 and ***P<0.001.

Fig. 10.

Effect of naringenin on the caspase-3 marker of I/R induced mice. Dennett’s test was used for statistically significance *P<0.05, **P<0.01 and ***P<0.001.

Fig. 11.

Effect of naringenin on the CPR level of I/R induced mice. Dennett’s test was used for statistically significance *P<0.05, **P<0.01 and ***P<0.001.

Pakistan Journal of Zoology

October

Vol. 54, Iss. 5, Pages 2003-2500

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