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Assessment of PCR-Based DNA Fingerprinting Techniques as a Novel Approach for Genotyping of Brucella Strains in Egypt

Assessment of PCR-Based DNA Fingerprinting Techniques as a Novel Approach for Genotyping of Brucella Strains in Egypt

Nour H. Abdel-Hamid1*, Walid Elmonir2, Eman I. M. Beleta1, Rania I. Ismail1, Momtaz Shahein1, Mahmoud E. R. Hamdy1 

1Brucellosis Research Department, Agricultural Research Center, Animal Health Research Institute, P.O. Box 264-Giza, Cairo 12618, Egypt; 2Department of Hygiene and Preventive Medicine (Zoonoses), Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt.

*Correspondence | Nour Hosny Abdel-Hamid, Brucellosis Research Department, Agricultural Research Center, Animal Health Research Institute, P.O. Box 264-Giza, Cairo 12618, Egypt; Email: nour78_78@yahoo.com 

Figure 1

Differentiation of Brucella at species by AMOS-PCR. Lane 1 B. melitensis reference strain Ether; lane 2, DNA ladder; lane3, control negative; lane (4-13) B. melitensis field strains. 

Figure 2

Differentiation of Brucella at species level by AMOS-PCR. Lane 1 B. abortus reference strain 544; lane 2, DNA ladder; lane3,4,5,7,8 B. abortus field strains; lane (6) control negative. 

Figure 4

Cluster and genotypes analysis of RAPD-PCR fingerprints of 24 field strains of B. melitensis isolated in Egypt along with the reference strain Ether. Band profiles of each strain are corresponding with the lines of the dendrogram. Three major clusters 1,2 and 3 (similarity ~ 54%) are demarcated. The RAPD genotypes, clusters, Brucella species, and strain serial numbers are represented in the columns. 

Figure 5

Cluster and genotypes analysis of ERIC-PCR fingerprints of five B. abortus field isolated in Egypt along with the reference strain 544. Band profiles of each strain are corresponding with the lines of the dendrogram. Two major clusters 1 and 2 (similarity ~ 63%) are demarcated. The ERIC genotypes, clusters, Brucella species, and strain serial numbers are represented in the columns. 

Figure 6

luster and genotypes analysis of RAPD-PCR fingerprints of 5 strains of B. abortus isolated in Egypt along with the reference strain 544. Band profiles of each strain are corresponding with the lines of the dendrogram. Two major clusters 1 and 2 (similarity ~ 78%) are demarcated. The RAPD genotypes, clusters, Brucella species, and strain serial numbers are represented in the columns. 

Figure 3

Cluster and genotypes analysis of ERIC-PCR fingerprints of 24 field strains of B. melitensis isolated in Egypt along with the reference strain Ether. Band profiles of each strain are corresponding with the lines of the dendrogram. Three major clusters 1 and 2 (similarity ~ 62%) are demarcated. The ERIC genotypes, clusters, Brucella species, and strain serial numbers are represented in the columns.  

Advances in Animal and Veterinary Sciences

August

Vol. 12, Iss. 8, pp. 1410-1621

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