Age and Gender-Dependent in Nitric Oxide Postnatal Change Activity in the Rat Brain
Laila Ibouzine-Dine1*, Inssaf Berkiks1,2, Mouloud Lamtai1*, Hasnaa Mallouk1, Ayoub Rezqaoui1, Abdeljabbar Nassiri1, Abdelhalem Mesfioui1, Aboubaker El Hessni1
1Laboratory of Biology and Health, Neurosciences, Neuroimmunology and Behaviour Unit, Faculty of Science Ibn Tofail University, Kenitra, Morocco; 2Department of Psychiatry and Behavioral Neurobiology, University of Alabama at Birmingham Heersink School of Medicine, Birmingham, AL 35233, USA.
*Correspondence | Mouloud Lamtai and Laila Ibouzine-Dine, Laboratory of Biology and Health, Neurosciences, Neuroimmunology and Behaviour Unit, Faculty of Science Ibn Tofail University, Kenitra, Morocco; Email:
[email protected],
[email protected]
Figure 1:
Experimental design. Male and female Wistar rat pups were sacrificed by decapitation at each of Postnatal Days (PND) 1, 2, 3, 4, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17. The prefrontal cortex (PFC), hippocampus (HC), striatum (STR), and hypothalamus (HY) were separately dissected and homogenized for the measurement of NO concentrations by using the diazotization method based on the Griess reaction.
Figure 2:
The graphic shows the Kinetics of the NO release in the Pre-frontal cortex area from male and female animals, for each postnatal day analyzed (PND1 to PND 17). Error bars represent the standard deviation of the means. The significance level is 0.05.*p < 0.05.
Figure 3:
The graphic shows the Kinetics of the NO release in the Striatum area from male and female animals, for each postnatal day analyzed (PND1 to PND17). Error bars represent the standard deviation of the means. The significance level is 0.05.*p < 0.05.
Figure 4:
The graphic shows the Kinetics of the NO release in the Hippocampus area from male and female animals, for each postnatal day analyzed (PND1 to PND 17). Error bars represent the standard deviation of the means. The significance level is 0.05.*p < 0.05.
Figure 5:
The graphic shows the Kinetics of the NO release in the Hypothalamus area from male and female animals, for each postnatal day analyzed (PND1 to PND 17). Error bars represent the standard deviation of the means. The significance level is 0.05.*p < 0.05.