Zafar Iqbal1* and Muhammad Khurshid2
...capture the ToLCNDV, two antisera raised against Tomato yellow leaf curl virus-coat protein (TYLCV-CP) and African cassava mosaic virus-coat protein (ACMV-CP) were used. However, immunocapture of ToLCNDV could only be achieved by using the TYLCV-CP antisera followed by PCR detection by using specific and degenerate primers. ToLCNDV is geographically wide spread Begomovirus (Family Geminiviridae), considered as a close relati...
Housh Muhammad Solangi1, Javaid Ali Gadahi1*, Mansoor Tariq2, Bachal Bhutto1, Zubair Ahmed Laghari1, Jamila Soomro3, Taufeeq Ahhmed Khosa1 and Abdullah G Arijo1
...s were recognized by the antisera produced by using the HcCP as antigen. Infective stage of the H. contortus (L3) was used for the challenge infection. Protein band pattern ranging from 10 to 170 kDa was observed and protein bands at 10-20 kDa, 24, 26 and 40-60 kDa were recognized by the antibodies against HcCP through western blot. Feacal egg count reduction test (FECRT) revealed that egg production was significantly reduced as 75.06%, 76.36% and 57.01...
Allam A. Megahed1; Hoda M.A. Waziri2; Khaled A. El-Dougdoug3; Badawi A. Othman3; Sirag M. Lashin1; Mohamed D. Hassanin1 and Mahmoud A. Ibrahim4
... technique. The produced antisera were evaluated and compared with foreign antisera using tissue printing immunoassay (TPIA) and dot blot immunoassay (DBIA). The produced antisera were found more efficiency in development the purplish blue color than the positive reaction when the foreign antisera applied, therefore it should be use produced
M. A. Kararah1, Om-Hashem M. El-Banna1, Salwa N. Zein2 and Abd-Elrehiem, A.F.3
...iters of the
antisera after first, second and third bleeding were 1/800, 1/1600 and 1/3200
respectively.The optimum concentrations of IgG and IgG conjugate were 1.0
μg/ml and 1/1000, respectively. The antigen dilution end point was
1:500.The produced antiserum was evaluated by ELISA and DBIA. Electron
microscopy f ultrathin-section of PMV- infected leaf tissue revaled several
morphologi...
A. A. Rezk1,3, K. A. Alhudaib2 and A. M. Soliman2,3
... (ELISA). The conjugated antisera that prepared in this study were succeeded to detect the virus in infected plants....
Aly M. Abdel-Salam
...as used for induction of antisera for CYSDV and CVYV. RT-PCR was used to amplify coat protein genes for the two viruses using specific primers. DAS-ELISA and immuno-blotting were used for evaluating the induced antisera. Results: Antisera for CYSDV and CVYV were produced efficiently and used for virus diagnosis through DAS-ELISA, DBIA, and TBIA. RT-PCR confirmed the nature of the two virus...
Dalia M. Omar1, Nermeen A. Marden1, Lamiaa M. Gaafar1, Elham A. El-ebiary1, Khalid El-Dougdoug2, Badawi Othman2, Abd Elsattar Arafa3, Hussein A. Hussein4
...n (HI) using standard AI antisera for H5 antigen and genetically by RTPCR
using specific primer for H5 gene and it was confirmed to be H5N1 and grouped according to the
year of isolation as 2016 isolate strain.
Results: According to the phylogenetic analysis it falls into the classical group of Egyptian AI viruses
subclade 2.2.1.2 which is dominant in Egypt since 2012 till now. Therefore, the virus was identified...
Akram I. Aboelkhair1, Ayman H. El-Deeb2, Momtaz A. Shahin1 and Hussein A. Hussein2
... ( SNT ) using reference antisera which confirm
presence of LSDV . By nucleotide sequences and genetic characterization was conducted on (G-PCR)
gene segment of these isolates.
Results: 93 samples were positive for LSDV by using conventional PCR direct from nodular lesion, 4
positive samples were inoculated on CAM showed characteristic pock lesion, formerly after passaging
of samples on MDBK cell line...
Nazia Haider, Asia Bibi* and Nahid Kausar
Shawki, Khaled K.; Carter, M.J.; Alnashar, Nariman M., El-Farrashl, Mohamed A. and Taher, Sahar
...ure studies by producing antisera directed predominantly at the most conserved S-domain of Norovirus capsid....
Omarl , Dalia M.; El-Ibiaryl , Elham A.; Sadik2, Atef S.; Abdel-Ghaffar2, Mamdouh. H. and Othman2, Badawy A.
...t (HI) using standard Al antisera and genetically by RT-PCR using specific primer. All the isolates were confirmed to be H5N1 and grouped according to the year of isolation as 2006, 2007, 2008, 2009 and 2010 group isolates. The HA titers of the above mentioned isolates were 6, 7, 8, 7 & 7 respectively. All the isolated Al viruses were titrated in ECE and examined for determination of their pathogenicity in specific pathogen free (SPF) 4-6 week old chicken....
Zein Salwa N; Abd El-khalik, Samaa; Khatab , Eman A.A.H and Azzam4,Clara R.
...m. Titer of the prepared antisera as determined using ELISA was 1/2000. Electron microscopic examination of infected leaves of N. clevelandii founed various cytological abnormalities. Due to the non-availability of sources of resistance in Egypt to TMV-S in sunflower, a mutation breeding program was initiated. Seeds of two genotypes were subjected to four doses of gamma rays; O, 100, 200 and 300 Gy from a 60Co source. Ml and M2 generations were sown at the exp...
Abdel Razek,B.Omar*and Magda M.Sayed**
...ion of the prepared LSDV antisera was 0.8g/dl. Separation of anti-LSDV immunoglobulins 1gG were done using ammonium sulphate followed by conjugation with fluorescein isothiocyanate at pH 9.6. The anti-LSDV IgG conjugated fluorescein sterile and was used to detect LSDV in the MDBK cells and gave good results to dilution 1/20 while the reference conjugate to 1/30....
Sabry Y. M. Mahmoud; Maher H. Hosseny and Mamdouh H. Abdel-Ghaffar
... assay (DAC-ELISA) using antisera against PVY, Potato virus X (PVX) and Potato leaf roll virus (PLRV). The results indicated the occurrence of single and mixed infections of three viruses in potato plants. Survey results indicated highly distribution of PVY infected plants, which its yield was affected strongly. Tubers of potato plants which gave a positive reaction to PVY only were collected at harvest, stored in cold store room for three months and then plan...
Manal A. El-Shazly1, A. s. 2 Abdel Wahab and Salwa N. Zein3
...y. Titer of die prepared antisera as determined using indirect ELISA were 1/3000 and 1/8000 for TSWV and IYSV, respectively. Authentic and induced antisera for both TSWV and IYSV were used for virus detection using different serological diagnostic methods such as indirect ELISA and dot-blot immunoassay (DBIA) on nitrocellulose membranes....
Nahed A. Mohamed l, H. A. Hussein2, Fathia M. Mohamed1 and M. A. Shalaby2
...standard known anti-BVDV antisera revealed that three positive samples for the presence of BVDV antigen. In a trial for the isolation of the BVDV, 69 buffy coat samples were inoculated in MDBK cells, five selected samples that induced a clear cytopathic effect on inoculated cells were passaged for 7 times and subjected for further antigenic and molecular characterization using virus neutralization test and different formats Of PCR assays. The results revealed ...
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