Nirbhay Kushwaha, Achuit K Singh, Brotati Chattopadhyay and Supriya Chakraborty
Recent advances in geminivirus detection and future perspectives
...dizations like dot blot, squash blot and Southern blot techniques, polymerase chain reaction and other DNA polymerase-mediated assays, and microarray detection. Of these, microarray detection provides the greatest capability for parallel yet specific testing and can be used to detect individual or combinations of viruses with sensitivity comparable to ELISA. Methods based on PCR provide the greatest sensitivity among the listed techniques but are limited in pa...
Muhammad Zafarullah Khan*, Shah Saud Ahmad and Asif Nawaz
... (13%), potato (34%) and squash (29%). Similarly, majority of the respondents obtained urea (73%), SSP (54%), DAP (60%) and Nitro-Phos (55%) from the FSC whereas 91%, 99% and 96% respondents obtained Micron, Supper Silica and Gypsum from the concerned dealers. It is concluded that there was a gap in invitation of farmers for general body meeting and participation in elections by FSC members. FSC administration did not fulfill its obligation regarding provision...
Muhammad Adnan Khan1*, Majid Suhail Hashmi1, Ali Muhammad1, Muhammad Muneeb1, Haris Bilal1 and Gul Wali2
...y of orange date blended squash at different ratios, i.e. OD1 (orange 90% + date 10%), OD2 (orange 80% + date 20%), OD3 (orange 70% + date 30%), OD4 (orange 60% + date 40%), OD5 (orange 50% + date 50%) and OD6 (orange 40% + date 60%). Orange date blended squash was prepared by the combination of pulp: sugar: water, with 3: 4: 1 ratio respectively. All samples were tested for total soluble solids (TSS), pH, titratable acidity...
Tahira Jatt1, Ghulam Sarwar Markhand1, Lane Rayburn2, Ray Ming3, Mushtaque Ahmed Jatoi1 and Ameer Ahmed Mirbahar1,4*
... the traditional Fuelgen squash method. Results indicated that all seven commercial varieties and four wild types of date palm are diploid in nature and having 2n=36 chromosomes. First time detailed karyotype of these varieties have been developed which includes total chromosome length (TCL), relative length (RL) and centromeric index (CI). The chromosome complement consists of metacentric (m), sub-metacentric (sm), sub-telocentric (st) and telocentric (t) chr...
Muhammad Ibrahim Kubar1, Fahad Nazir Khoso1*, Imran Khatri1, Niaz Hussain Khuhro2 and Arfan Ahmed Gilal1
...rd, sponge gourd, Indian squash and bitter gourd were designed in Randomized Complete Block Design (RCBD) having 16 treatments with 5 replications each. Basically, there were five treatments; T1= Untreated control, T2 = Spinosad (Tracer), T3= Cue-lure, T4= Protein hydrolysate, T5 = Trybliographa daci. Furthermore, combinations of these treatments were designed to find out the most effective combination. The results revealed that among the solo treatments, the ...
Amro A. Farrag1; Ahmed K. El-Attar1; Om-Hashem M. El-Banna2; Ibrahim A. I.2; H.
M. Mazyad1
Amro A. Farrag1; Ahmed K. El-Attar1; Om-Hashem M. El-Banna2; Ibrahim A. I.2; H. M. Mazyad 1
Amel, S.M. Abo-Senna1; M.A. Nasr-Eldin2; B.A. Othman3 and A.A. Megahed4
...resh weight and yield of squash plants....
Muhammad Jan1*, Muhammad Ashraf Sumrah1, Shoaib Akhtar1, Inam Ul Haq2, Muhammad Ramzan Anser1 and Iqra Yasmin1
...ts like pickles jams and squash enforcing the farmer to produce more and more. The increasing demand creates the competition among the farmers. To produce more it is important to apply fertilizer at a right time, right source and right method. In case of soil application, apply first dose of nitrogen, all phosphorus and all potassium fertilizers during the December. Apply second dose during May-June and third dose during September in Pothwar. Foliar applicatio...
Atef El-Sagheer1*, Anas El-Mesalamy1, Abdel-Monem Anany2 and Nashaat Mahmoud1
...st M. javanica infecting squash plants under greenhouse conditions. Data indicated that treatment with P. guilliermondii filtrate (1012) caused best percentage of nematode reduction (71.46%). Followed by S. roseus at (1012) (42.95 %), then in third treatment with C. albidus (103) by (40.50 %). On the other hand, all tested treatments decreased the negative effects of nematodes and enhanced growth characters of squash plants....
Elbeshehy, E. K.F. and Sallaml, A.A.A.
... plant species including squash, pumpkin, pepper, bean, Chenopodium amaranticolor and cowpea showing foliar symptoms of mosaic, deformations and necrotic and chlorotic ring spots, that resemble those induced by CMV. SDS-PAGE test showed various distinguishable sole novel protein bands in four cucumber cultivars infected with CMV but not in healthy one. RT-PCR, with the primer CMV 1 and CMV2 for CMV-CP. gene, yielded 422 base pair DNA fragments. The following s...
M. Osman1, Kh. El-Dougdoug2, E. T. Abd El-Salam3, R.M. Taha4 and R.M. El-Hamidl
...solate was propagated on squash plants var. Eskandarani. After 15 days. systemic symptoms appeared in the newly formed leaves in the form of vein banding, severe mosaic, yellowing, malformation, and leaves wilting. Histopathological studies of CMV-infected squash leaves showed changes such as compact mesophyll, disintegrated and less lignified metaxylem, slight upward blistering of leaf blade, closed stomata, short palisade ...
Wagner Antonio Gorozabel Muñoz1*, Mayra Jossenka Loor Solórzano1, Josselyn Gema Palacio Intriago1, Virginia Vanessa Andrade Andrade1 and Carlos Alfredo Cedeño-Palacios1,2
...dorian industry, such as squash. This was accomplished by evaluating its properties and conducting physicochemical, antioxidant, and beta-carotene analyses on the flours obtained from the pulp of three squash varieties: vermilion squash, pepo and macre. For the research project, the squash samples were subjected to a process of selection, classification,...
