Antibacterial Activity by Chlorogenic Acid Isolated through Resin from Apricot (Prunus Armeniaca L.)
Ahmed Mujtaba1, Tariq Masud1, Asif Ahmad1, Waqar Ahmed3, Saqib Jabbar3* and Robert E. Levin2
...food borne pathogens and quantification of the chlorogenic acid (CA) using High Performance Liquid Chromatography (HPLC). The chlorogenic acid was isolated using resin. The recovered amount of compound was calculated. Aliquots of the extracts at known concentration of chlorogenic acid were then applied against different bacterial strains for antibacterial activity. The minimum inhibitory concentration of isolated compound was also measured. Two antibiotics wer...
Ghulam Abbas1,Asif Nadeem1,*, Masroor Ellahi Babar2, Tanveer Hussain2, Muhammad Sajid Tahir3, Wasim Shehzad1, Rajput Zahid Iqbal3, Muhammad Tayyab1 and Maryam Javed1
...After DNA extraction and quantification, amplification of gene was done by polymerase chain reaction. PCR products were sequenced bi-directionally, aligned and single nucleotide polymorphisms were identified. Bioinformatics tools were applied for construction of phylogenetic tree and genetic diversity analysis. Lower genetic diversity was observed. The finding of this research is prerequisite for future research.
...
Samiah Shahid1,Jawaria Shaheen1, Wajeehah Shahid2, M. Waheed Akhtar1,3 and Saima Sadaf1,*
...se of reference gene for quantification of circulating miRNAs through qPCR analyses in ALL. In the current study, we identified the reference gene that is the most suitable for qPCR normalization in patient and control plasma samples of ALL. Three highly reported reference genes namely RNU6-2, mir-16-5p and cel-mir-39-1 were selected as the candidate genes for normalization. Preliminary, quantification was performed t...
Huma Naz1*, Sajid Abdullah2, Khalid Abbas2, Muhammad Rizwan Tariq3, Laiba Shafique4, Ghazala Nazeer5
Using Smartphone Application to Estimate the Defoliation Caused by Insect Herbivory in Various Crops
Muhammad Irfan Ullah1*, Muhammad Arshad1, Sajjad Ali2, Asad Abdullah1, Samina Khalid3, Hafiz Muhammad Aatif4, Syed Muhammad Ali Zahid1, Muhammad Afzal1 and Jaima Molina-Ochoa5
...synthesis process. Thus, quantification of foliar area needed for the photosynthetic process is impartment. The recent methods used to quantify the foliar damage are relatively expensive and are mainly dependent on complex and state of art devices and instruments. Instead, smartphone application may be a good source to measure the foliar damage. In this study, foliar damage of Spodoptera litura Fab. (Lepidoptera: Noctuidae) on sunflower and soybean crops was m...
Muhammad Asif Farooq1*, Muhammad Jalal Arif1, Muhammad Dildar Gogi1, Bilal Atta2 and Ahmad Nawaz1
... preparation followed by quantification with GC-ECD. Recoveries of the samples analyzed were ranged from 78-98%. Results revealed that 75.00% samples of pesticide residues mitigation in PRMM-4 followed by PRMM-3 (66.66%), PRMM-2 (33.33%) and PRMM-1 (25.00%). Similarly, the highest number of samples (41.66%) from PRMM-4 exceeded maximum residual limit (MRL) values of the Codex Alimentarius Commission while the lowest (16.66%) were observed in PRMM-1. In additio...
Yasir Irfan Badrashia1, Qaisar Alia1, Mohammad Ashraf1, Muhammad Rashid1
SEISMIC DESIGN CHARACTERIZATION OF RC SPECIAL MOMENT RESISTING FRAMES IN PAKISTAN-FIELD SURVEY TO LABORATORY EXPERIMENTS
...dentification and
quantification of material and detailing disparities between design specifications and construction practices. The effect
of these disparities was studied using quasi-static cyclic testing on two exterior beam-column connections; EJ-1A
(code-compliant) with no deficiencies and EJ-2A (non-compliant) incorporating all identified deficiencies. Damage
patterns and hysteretic force-deformation behavior of these models i...
quantification of material and detailing disparities between design specifications and construction practices. The effect
of these disparities was studied using quasi-static cyclic testing on two exterior beam-column connections; EJ-1A
(code-compliant) with no deficiencies and EJ-2A (non-compliant) incorporating all identified deficiencies. Damage
patterns and hysteretic force-deformation behavior of these models i...
Zahid Hassan Tarar1, Muhammad Salik Ali Khan2*, Shahzada Munawar Mehdi3, Raza Salim4, Irfan Ahmad Saleem1, Saima Nazar5, Munir David2, Tahir Majeed6, Muhammad Sufyan Mughal7, Muhammad Saleem8, Umer Iqbal9, Muhammad Mazhar Iqbal10 and Muhammad Khalid Shaheen3
Iram Liaqat1*, Tahir Hussain1,2, Aisha Waheed Qurashi3, Gulbeena Saleem2, Asia Bibi4, Muhammad Fiaz Qamar5,ShaukatAli1 and Ikram-ul-Haq6
...e coverslip, (2) biofilm quantification at different time intervals and (3) monitor antibiofilm effect of three proteolytic enzymes including trypsin, chymotrypsin and proteinase k against S. Gallinarum. We observed that S. Gallinarum has strong biofilm forming ability as observed by dark black colonies on congo red medium. Quantification assays such as test tubes revealed significantly (p<0.001) strong biof...
D. N. Abd-elshafy1, 2 and M. M. Bahgat2, 3
...leupeptin and EDTA. Also quantification of the activities of both intracellular and secreted HepG2 proteases was carried out using specific chromogenic substrate at different ranges of pH values. Results showed that EDTA has intracellular metalloprotease inhibitory effect that enhances rate of HAV replication and thus reduces time needed for the virus to cause cytopathic effect. Such finding will definitely facilitate the detection of the virus both in clinica...
Lamiaa M. Omar, Mohamed A. Abdrabo, Dali M. Omar, Nermeen A. Marden
... for virus isolation and quantification by ECE inoculation and rRT-PCR.
Results: the titer of original HPAI virus was 1010.3 EID50/ml. The AI titers after experimental
infection of chicken and viral re-isolation were 1010.3 EID50/ml in ECE and 1.6×107 copies/ml by
rRT-PCR to sample (1), 105.4 EID50/ml in ECE and 1.2 ×106 copies/ml by rRT-PCR to sample (2),
and 106.2 EID50/ml in ECE and 4×105 cop...
Zhi-Ming Luo*, Xiao-Yan Wang, Jiong Yin, Ying-Kun Huang, Wen-Feng Li, Rong-Yue Zhang, Hong-Li Shan, Xiao-Yan Cang and Jie Li
Nagarathinam Arunkumar*, Jainullabudeen Gulsar Banu, Nagarajan Gopalakrishnan and Arkalgud Hiriyannaiah Prakash
...mL, respectively, in the quantification process. In addition, the growth curve analysis confirmed that lipase production is associated with the growth phase of the isolates. The efficacy of the lipase producing wax degrading bacterial isolates was tested against the nymphs and adults of Solenopsis Mealybug, Phenacoccus solenopsis Tinsley and the Striped Mealybug, Ferrisia virgata Cockerell infesting cotton. The results clearly indicated that the ...
Luis Daniel Jiménez Martínez1, Vicente Morales Garcia2, Carlos Alfonso Frias Quintana3, Alejandra del Carmen Castillo Collado1, Gloria Gertrudys Asencio Alcudia4, Carina Shianya Alvarez Villagomez4, Emyr Saul Peña Marín4,5, Bartolo Concha Frias1 and
Carlos Alfonso Alvarez-Gonzalez4
...and genomic studies. The quantification of gene expression through RT-qPCR is one of the most commonly used techniques, due to its precision, sensitivity and high performance, particularly in gene expression to compare between cells, tissues and organs; as well as different populations, stages of development, metabolism, among other conditions. This study analyzed the stability and normalization of six commonly used reference genes such as alpha elongation fac...
Longjun Guo*, Jiaqi Teng, Juan Wang and Yukun Chen
...ion tensor imaging (DTI) quantification and cerebrospinal fluid τ (Tau) protein expression in leukoaraiosis patients. Inpatients who attended the Department of Neurology of our hospital from January 2018 to December 2018 were selected as study subjects, with 58 patients with leukoarais as the experimental group, and 60 patients without leukoarais in the same period as the control group. ELISA was used to detect τ (Tau) protein expression level in the c...
Yan Jiang1,2, Xia Tao3,4 and Hongxia Chen5,6*
...es such as urine protein quantification (Upro), blood creatinine (Scr), urea nitrogen (BUN)), serum albumin (ALB), nephrin, and BAFF levels before treatment, 3 months after treatment, and 6 months after treatment were compared between the 2 groups. Results showed that after treatment, the total effective rate of the study group was 93.48% higher than that of the control group 76.09% (P<0.05). The clinical symptom scores of the two groups were lower than tho...
Pham Tan Nha1*, Nguyen Thi Kim Dong2, Le Thu Thuy1
... showed that ginger made quantification of triglycerid and quantification of total cholesterol in chicken blood decrease.
Keywords | Tau Vang chicken, Ginger, Triglyceride, Cholesterol and digestion
...
Aqsa Fayyaz1, Muhammad Kashif Zahoor1*, Asma Ashraf2, Muhammad Asif Zahoor3, Azhar Rasul2, Humara Naz Majeed4, Muhammad Zulhussnain1, Kanwal Ranian1, Bushra Riaz1, Nazia Khalil1 and Attaullah1
...ion inhibition assay for quantification of NDV. Polyclonal antibodies were produced in experimental rabbits followed by titration. These antibodies were then used to detect the presence of NDV. Sixteen samples were found positive (20%), 62 negative (77.5 %) and two unreactive (2.5 %) for NDV. All of the poultry farms around Faisalabad showed positive NDV (ranging from 1 to 4 samples). We conclud that house flies can play a role in the mechanical transmission o...
Deepesh Kumar Bhuptani1, Atta Hussain Shah1*, Gul Bahar Khaskheli1, Zubair Ahmed Laghari2, Ghulam Shabir Bahram1, Muneer Ahmed Jamali1, Tarique Ahmed Khokhar1
...ducted on prevalence and quantification of AFM1 in raw buffalo milk in Sindh province. A total of three hundred and fifteen raw milk samples (n=315) from dairy farms of three regions in Sindh province i.e., southern (Karachi, Thatta, Hyderabad), central (Mirpurkhas, Umerkot, Shaheed Benazirabad) and northern (Naushahro Feroze, Sukkur and Larkana) were collected and analyzed using commercial ELISA kit. The results revealed that AFM1 was statistically higher (PË...
Muhammad Mansoor1, 2, Sheheryar1 and Shahid Ali Khan1*
...Among the goals were the quantification of mungbean flowering flushes to final yield, identification of short duration variety, to recognize varieties suitable for water-deficient areas, and varieties suitable for cotton crop intercropping before reaching their maximum canopy level. The analysis was carried out in randomized complete block design (RCBD) with three replications of split plot arrangement; varieties (NM-2011, Inqelab Mung, Dera Mung, and NM-98) w...
Muhammad Mansoor1, 2, Sheheryar1 and Shahid Ali Khan1*
...Among the goals were the quantification of mungbean flowering flushes to final yield, identification of short duration variety, to recognize varieties suitable for water-deficient areas, and varieties suitable for cotton crop intercropping before reaching their maximum canopy level. The analysis was carried out in randomized complete block design (RCBD) with three replications of split plot arrangement; varieties (NM-2011, Inqelab Mung, Dera Mung, and NM-98) w...
Emel Hulya Yukseloglu1, Fatma Cavus Yonar1*, Omer Karatas1, Gulten Rayimoglu1, Faruk Asicioglu1, Elif Canpolat1, Onur Ozturk2 and Itir Erkan3
... dynamic range, limit of quantification, stochastic threshold study, reproducibility and repeatability, mixture and contamination study parameters. Validation parameters and population genetics data were calculated by using Arlequin v 3.5.2.2 version. According to our results, SE33 (PM=0.014) locus showed the greatest power of discrimination and TPOX (PM=0.132) has the least power of discrimination power in Turkish population. Combined power of exclusion and c...
Marwa S. Khattab1*, Ahmed H. Osman1, Huda O. AbuBakr2, Rehab A. Azouz3, Asmaa A. Azouz4, Heba S. Farag5
... were below the limit of quantification in 3 samples only. Histopathology of tick-infested skin revealed severe multifocal eosinophilic dermatitis and inflammation of subcutaneous hypodermis (panniculitis) whereas, in the skin with high ivermectin residue, there was mild multifocal epithelial hyperplasia with mild perivascular mononuclear cells infiltration. Conclusion: Different levels of ivermectin residue were detected in bovine skin samples collected from ...
A. A. Galal1,2, S. F. Abou-Elwafa1,3, F. J. Kopisch-Obuch1 and C. Jung1
Shaista Naz1*, Muhammad Arif2 and Akhtar Ali3
...time allocation studies (quantification) on the subject matter were found scarce. It is therefore recommended that more empirical research should be carried out on time allocation studies and determinants of women’s participation in the livestock management as it will not only better quantify and recognize women’s role in the sector but will also be helpful in designing more effective, women’s specific policies and programs in the sector....
MUHAMMAD WAHEED MUSHTAQ1*, NAUMAN JAMIL KHAN1, NAVEED ASLAM DOGAR1, AISHA HAMEED2, ABDUL HAMEED2, MALIK ADIL2, HAROON IFTHIKHAR3 & SAIRA ISHAQ4
Wuritunashun*, Burenbatu, Narenqiqige, Jiuguniang, Eerdunduleng, Shuanglian Wang, Cuiqin Gong, Hashengaowa, Huizhi Jin, Baiwurihan and Chunhaizi
...es. We used a label-free quantification (LFQ) proteomics approach to investigate the effect of the Zhenbao pill on the differential serum protein expression of HSP patients. Out of 14 significantly differentially expressed proteins, four proteins immunoglobulin lambda variable 3-27, immunoglobulin kappa variable 3-11, neural cell adhesion molecule L1-like protein, and immunoglobulin heavy variable 3-33 were upregulated, whereas 10 proteins i.e., protein Z-depe...
Ijaz Ahmad1,5*, Haihong Hao1, Pascal Sanders2, Zafar Iqbal3, Saeed Ahmed4, Farhan Anwar Khan5, Zahir Shah5, Muhammad Ibrahim6 and Lingli Huang1
...micro;g/mL. The limit of quantification (LOQ) and limit of detection (LOD) were 0.04 and 0.01 µg/mL. The recovery of cefquinome from plasma was 73.4%, 78.33%, and 77% for low, medium and high concentration samples. The intraday and inter day relative standard deviation were less than 15%. The Cefquinome were found to be stable for more than 3 month at -70oC (degree symbol) and more than 24 h at 4oC. The present method has been applied successfully for th...
Sameh Abdel-Moez Ahmed Amer1*, Aly Mohammed Ghetas1, Asmaa Mahmoud Maatouq1, Hagar Magdy Ahmed1, Khaled Mohamed El-Bayoumi1, Mohamed Abd El-Rahman Bosila1, Ahmed Ali El-Shemy2
...immune response and also quantification of virus shed post-challenge (PC) via real-time QRT-PCR were all screened and precisely recorded. The results revealed that, group A chickens developed the highest humoral antibody titers throughout the vaccination schedule and conferred a complete protection against mortality with milder clinical signs, moreover displayed a significant decrease in the shedding of NDV with drastically blocked shedding 7 days PC compared ...
Samia Afzal1*, Jahanzaib Ahmad1, Iram Amin1, Liaqat Ali2, Muhammad Shahid1, Mohsin Ahmad Khan1 and Muhammad Idrees1
... 58% of the samples. The quantification of viral load revealed chronic active HBV infection at 11% and chronic inactive HBV infection as 63%. HDV RNA was present in 41.2 % of inactive chronic HBV carriers and only 18.1% in active HBV-infected patients. I has been observed that the presence of HDV infection may suppress the HBV replication and convert it into an inactive state and the overall prevalence of HDV in Pakistan is 35% in HBV-positive patients. <...
FARZANA SIDDIQUE*1, NAZIMA FIRDOSE2, MUHAMMAD ARSHAD2, WARDAH HASSAN2
& SHAFAAT YAR KHAN2
Saloua Ziani1*, Khalid Sohaib2 , Youssef Lhor3, Ikhlass El Berbri4, And Ouafaa Fassi Fihri4
...untries require a rabies quantification before importing animals, which varies significantly. The objective of this study is to investigate the reasons for variations in antibody titers in dogs. Data from 848 dogs exported from Morocco was collected. The results revealed that too-old dogs (over six years) or too young (under four months) are also less likely to respond to vaccination than dogs that are four months to six years old. A dog receiving a booster do...
Nauman Jamil Khan1, Rabia Tariq2, Hina Saleem2 and Muhammad Waheed Mushtaq2*
Mahdyl , A.M.M.; Hafezl , M.A.; EL-Dougdoug2, Kh. A.; Fawzyl , R.N. and Shahwanl , Eman S.M.•
...he obtained results from quantification of total SA in induced tomato plants (after 7 days of spraying inducers) showed high level of SA with kombucha &eatment followed by C. inerme and M. jalapa, while mixed (M. jalapa+C. inerme) gave the lowest level of SA compared with healthy and infected controls. On the other hand, after virus inoculation tomato plants showed reduction of virus and disease severity. M. jalapa, C. inerme and kombucha showed lowest per...
Muhammad Ather Nadeem1, Bilal Ahmad Khan*1, Saima Anwar2, Hasnain Abbas3, Muhammad Yasin1, Rizwan Maqbool3, Muhammad Mohsin Amin1, Amir Aziz5, Muhammad Sikander Hayyat1, Muhammad Shozib Javed5
...ocloa cruss-galli L. and quantification of allelochemicals present in leaves, stem and fruit of S. oleraceus weed. The experiment comprised of aqueous extracts of leaf, roots and fruits applied at 0.25, 0.5, 1, 2, 4 and 8% (w/v) along with a control (0%). Data regarding seed emergence, emergence index, emergence percentage, root length, shoot length and seedling dry weight were recorded. All the tested concentrations of all the parts of S. oleraceus significan...
