Qingzhong Yu
...tigate the most suitable insertion site for optimal expression of a foreign gene without compromising the replication of the Newcastle disease virus. Recently, inserting a green fluorescent protein (GFP) gene upstream from the GE sequences of the viral genes and subsequent quantitative measurements of the GFP fluorescence intensity, we have concluded that the P and M junction region is the optimal insertion site for a high l...
Hussein Aly Hussein1*, Omneya Mohamed Khattab2, Shereen Mohamed Aly2, and Mohammed Abdel Mohsen Rohaim1
...V strains, 21 nucleotide insertion and 12 nucleotides deletions were identified in the GPCR genes of our isolates and other LSDVs. The amino acid sequences of GPCR genes of our isolates contained the unique signature of LSDV (A11, T12, T34, S99 and P199). Phylogenetic analyses showed that the GPCR genes of LSDVs identified from ticks were closest genetically to the previously detected LSDVs from infected ruminants, indicating a potential role of Ixodid ticks f...
Muhammad Abid1*, Tahir Yaqub2, Arslan Mehboob3 and Muhammad Zubair Shabbir4
... isolates illustrated no insertion, deletion and substitutions compared to A/DK/HK/Y280/97 lineage and human isolates (A/HK/1073/99 and A/HK/1074/99). Several amino acid substitutions at various regions of the NA protein were determined with unknown functions and consequences. These findings warrant future studies for analysis of whole genome sequencing of prevailing H9N2 viruses in Pakistan which may further contribute towards the better understanding of the ...
Tazeen Jamil, Saba Ijaz, Rabia Arif*, Faiza Akram and Muhammad Saleem
...ryotic genomes and their insertion in coding regions leads to altered gene expression. We examined the diversity of retroelements in four consecutive generations of different strains of Sordaria fimicola. The parental strains werecollected from two contrasting environments of Evolution Canyon i.e. harsh South facing slope and neutral North facing slope. Retroelements profiles were generated in order to detect retrotransposons. Strains from South ...
Bingbing Gao1, Na Song1, Zhonglu Li2, Tianxiang Gao3 and Liqin Liu3,*
...for genetic analysis. No insertion or deletion were found among 26 mutation loci and 27 haplotypes were detected. All six populations were characterized by low haplotype diversity (0.39–0.59) and low nucleotide diversity (0.00038–0.00073). The result of AMOVA and pairwise Fst values indicated weak or no genetic differentiation among different populations. Haplotype network and NJ phylogenetic trees revealed that there was no disti...
Iram Gull*, Muhammad Shahbaz Aslam, Imran Tipu, Roohi Mushtaq and Muhammad Amin Athar
...ess gene fusion with the insertion of sequence of choice at junction sites has been described by optimization of various parameters of overlap extension PCR. The fusion of IFNα2b gene to the gene of human latency associated protein with insertion of HCV NS3 protease cleavage site at splicing junction is reported.
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Abid Hussain Shahzad1, Abdul Sattar1,*, Nasim Ahmad1, Ijaz Ahmad2, Deniz Nak3 and Yavuz Nak3
... except the intravaginal insertion of controlled internal drug release (CIDR) inserts for 5 or 7 days, respectively. Pregnancy was diagnosed using ultrasonography on d30, d60 and d90 post AI. Ovulatory follicle diameter was measured at timed AI and progesterone profile (ng/mL) on d30 and d60 post AI. Pregnancy rate was analyzed by Chi-square procedure while ovulatory follicle diameter and Progesterone profile by one way ANOVA (α=0.05). Ovulatory follicle...
Tasbihullah1, Sadeeq ur Rahman2,*, Tariq Ali3 , Umer Saddique1, Shakoor Ahmad1, Muhammad Shafiq4, Sultan Ayaz2, Hamayun Khan1, Ijaz Ahmad1 Asadullah2, Raheela Taj5, Hafiz Nidaullah6 and Alamgir Khan7
... isolates were harboring insertion sequence common region 1 (ISCR1), which was found to be linked with blaCTXM in 50% (4/8) isolates. Results of the current study indicate contamination of eggs with ESBL-producing E. coli suggesting to improve hygienic process for end consumer during- egg production.
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Masroor Ellahi Babar*
...otide deletion, AT bases insertion and transvertions (C>G; G>T). Deletion of A bases at c.1-1075 and hetrozygosity (CT) at the c1-1046 were analyzed along with some other SNPs are very important. These evidences points towards the change of expression of those protein which are nearest to the region of gene regulation and enhancer region. After observing taxas several distinct clads of camel breeds it was found that Kharani breed showed the close relatio...
Mahmoud Mohamed Ahmed Youssef* and Suzan Abd-Elazeim Hassabo
The Role of Genetic Engineering in Management of Plant Parasitic Nematodes with Emphasis on Root-Knot Nematodes: A Review
...le material (DNA) by the insertion of nucleic acid molecules produced outside the cells into any virus, bacteria, plasmid or other vector system in which they are capable of continued propagation. Azotobacter spp. with nematicidal activity could control root-knot nematode, Meloidogyne incognita. The development of such strains by genetic manipulation by transferring gene (s) required for saponins production is effective in increasing crop yield together with f...
Muhamamd Rizwan1*, Muhammad Arshad2, Muhammad Kashif3, Aneela Zameer Durrani4, Asghar Abbas5, Tanveer Ahmad6, Muhammad Nadeem7 , Kinza Khan8
Aya El-Turkey1, A. K. El-Attar1, A. E. Aboulata1, B. Othman2 and K. A. El-dougdoug2
...e tested for the HSV-2gD insertion and transcription using both PCR and RT-PCR and the results were successful in getting insert-containing tomatoes against HSV-2gD. Specific expression of the introduced antigen construct at the RNA level was detected by RT-PCR, using specific primers for the HSV-gD2 transcript. Expression of the HSV-2 gD antigen was assayed by ELISA on protein extracts isolated from same transfected plant tissue samples as for the RT-PCR assa...
Rehman Shahzad1, Saba Irshad1* and Faisal Amin2
...n related substitutions, insertion or deletions in hem-absorbing site and loop of H9N2 isolates. Two unique substitutions, Gln39Arg and Lys47Glu have been noted in NA stalk domain of all isolates of this study which might be indirectly enhancing the virulence of virus. These findings demonstrated that new mutations are emerging in B2 sub-lineage and there is need for constant surveillance of evolving genome of H9N2 virus prevailing in the country to combat the...
Om-Hashem M. EI-Banna1, M. A. S El-Kady2, E. A Salama1 and Salwa N. Zein2
...erent. three amino acids insertion, and one amino acid deletion. Little differences were also observed regarding the 5' UTR of RNA γa as six nucleotides were changed. 12 out of 162 amino acids were differed and one amino acid deletion. Gl19 strain cross reacted specifically with the monoclonal antiserum raised against the coat protein of ND18 stain and the two coat proteins were indistinguishable in their sizes....
A.S. Gamal El din1; Sohair I. El-Afifi2; A. S. Sadik 2; Nashwa M. A. Abd El-Mohsen1; and H. M. Abdelmaksoud1
Umer Farooq1,2, Nimra Murtaza2, Abubakar Siddique1, Bilal Saleem1, Obaid Ur Rehman1, Nageen Zahra1, Muhammad Uzair1, Muhammad Naeem Riaz1,3* and Muhammad Ramzan Khan1*
Amthal Ahmed Fouad1, Basem Mohamed Ahmed2, Momtaz Abdelhady Shahein1, Hussein Aly Hussein2*
...ein genes. Deletions and insertions were observed in the intergenic spaces. It was highly similar to Egyptian RABV genomes and clustered within the Africa-4 (AF4) lineage of the cosmopolitan clade. It was submitted to NCBI GenBank under the access number OL314495. Conclusion: The study provides analysis for the 5EG-QH19 wild-type RABV genome, elucidates some key structural features of the AF4 lineage dominant in Egypt and supports the recently proposed divisio...
Hemeida, A. A., Osman, M., El-Shahat, Mohamed, Hashem, Medhat H., Mahmoud, Amal and Dahi, Hosni
...72, and 180). Nucleotide insertion was seen in one sequence in the nucleotide position of 212. Root neighbor-joining (NJ) tree revealed that the three HCV samples could be classified as genotype 4. Two of the three samples were closely related (98.2%) while the third clustered with isolate from Gabon (98.7%). The third isolate may be a new subtype but this will require an in-depth exploration on sequence variability in at least two coding regions....
Sarah G. Yousef1*, Ahmed Shehta2, Hend M. El Damaty1 and Hussein A. Elsheikh3
...a. The PCR targeting the insertion sequence IS900 gene revealed that 22.6% of examined cattle were positive for MAP. A rate was significantly higher in diseased older cattle than in young cattle with no symptoms of the disease. Furthermore, DNA sequencing and phylogenetic analysis revealed that our strains (ON816021 and ON816022) were 100% identical showed complete identity with MAP-C (M. paratuberculosis of cattle origin) as Japanese (CP066812) and German str...
Tinda Afriani1*, Jaswandi1, Yurnalis1, Putri Oktavially1, I Made Merdana2
... C>T 1659 bp, and one insertion C 1838 pb. This study found two alleles and three genotypes with a general heterozygosity value where H0>He. The growth hormone gene from the Pesisir and FH cattle crosses were polymorphic, with imbalanced genotype frequencies, and the body size of males was longer than that of females.
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Gokarna Gautam1*, Hari Adhikari1, Bhuminand Devkota1, Subir Singh2
...icity status during CIDR insertion and the presence of corpus luteum (CL) on 8-9 day after FTAI were determined using transrectal ultrasonography. Estrus expression rate (96.7% vs 68%; P=0.008) and ovulation rate (93.3% vs 76%; P=0.10) were higher in CIDR-synch than in Ovsynch group. Although pregnancy rate from FTAI did not differ between CIDR-synch (33.3%) and Ovsynch group (16%), overall pregnancy rate obtained from FTAI plus natural breeding within one mon...
Ali D. Nashmi1*, Jawad K. Hasan1, Manal A. Ibrahim2
...ight lung was lavage, by insertion of a tracheal cannula, three times with 10 ml of normal saline (NS 0.9%) at 37 °C and the broncho alveolar lavage fluid (BALF) was sent for laboratory evaluations and the left lung was incised for histopathological evaluation. Data analysis indicated that a substantial decrease in total white blood cell counts (WBC) was observed (p-value <0.05) and cells including (neutrophils, mononuclear) in BALF of rats treated with...
Shruti Yadav1,2*, Kamana Singh2, Pratap Adinath Divekar3 and Suhas Gorakh Karkute1,3*
...lysis showed single copy insertions in plants of six independent transgenic events. Cry2Aa gene was highly expressed in transgenic plants and its protein level was as high as 30.94 µg/g in fresh leaves and 20.57µg/g in fruits. Insect bioassay showed the BSFB larval mortality between 90% to 100%. Altogether it was observed that expression of the Cry2Aa protein in the shoots and fruit of transgenic brinjal lead to high BSFB larval mortality. Thus, Cr...
