Hira Akhter1, Bilal Aslam2*, Naveed Shahzad3, Tanzeela Farooq4, Muhammad Umer5 and Muhammad Hidayat Rasool2
Aziz-ul-Rahman, Farooq Yousaf, Naveed Anwar and Muhammad Abubakar
Mumtaz Ali Khan1, Aneela Zameer Durrani1, Sher Bahadar Khan2, Shehla Gul Bokhari1, Ikramul Haq1,*, Imdad Ullah Khan3, Naimat Ullah4, Naimat Ullah Khan4, Kashif Hussain1 and Azmat Ullah Khan2
... evaluated with indirect haemagglutination test. The results indicated significantly higher (P< 0.05) immune titer in animal group vaccinated with toxoid vaccine prepared from pathogenic isolates and provide best protection against challenge infection.
... 
Ihsan Ali1#, Asim Aslam1*, Habib-ur-Rehman2, Beenish Zahid3, Ishtiaq Ahmed1
Effect of fish oil on growth performance and immune response in experimentally infected broiler chicken with avian influenza virus H9N2
...y up to 42 day of age by haemagglutination inhibition test. The
mean GMT for H9N2 of group A, B, C, and D after challenge were 76.0, 273.7, 0.5
and 0.35, respectively. However, after challenged the highest GMT for NDV was
observed in group C with 158.8, followed by group B, D and A with GMT of 72.6,
49.3, and 11.32, respectively. Congestion, depletion of lymphocytes, dysplasia of
thymic lobules, necrosis, disappearance of lym...
mean GMT for H9N2 of group A, B, C, and D after challenge were 76.0, 273.7, 0.5
and 0.35, respectively. However, after challenged the highest GMT for NDV was
observed in group C with 158.8, followed by group B, D and A with GMT of 72.6,
49.3, and 11.32, respectively. Congestion, depletion of lymphocytes, dysplasia of
thymic lobules, necrosis, disappearance of lym...
Ragia S. Mohamed1, Rania F. El Naggar2, Mamdouh. M. Hamoud1, Mohamed M. Hamoud3, Abdulrhman M. Gamal1, Samah E. Laban1, Shimaa A.E. Nasr1, Manal M. Zaki1, ElShaimaa Ismael1, Osama K. Zahran1*
...s of virus isolation and haemagglutination assay (HA). Our results revealed that pH of the manure from time of its mixing with the NDV infected allantoic fluid till day 4 was 7, then pH values changed progressively and recorded 8.5 on day 12. After that, pH increased towards the alkaline side and recorded pH = 9 till day 33 of the experiment. Regarding moisture %, after mixing the manure with allantoic fluid the moisture % of the mixture was 67%, and this wet ...
Fady Samir1, Rania F. El Naggar2, Mohamed M. Hamoud3, Manal M. Zaki1, Abdulrhman M. Gamal1, Samah E. Laban1, Shaimaa A. E. Nasr1, El Shaimaa Ismael1, Osama K. Zahran1*
...us isolation followed by haemagglutination assay (HA) which revealed that NDV survived in the manure up to 28 days. Likewise, monitoring for the manure pH and moisture % was carried out. Our explanation for the long-time course for NDV persistence due to increasing the moisture %. In this context, the findings of this study revealed that genotype VII viruses of class II NDV are circulating in Egypt. Furthermore, genotype II-based vaccines seem to be not effici...
Mumtaz Ali Khan1,*, Sher Bahadar Khan2, Shakoor Ahmad2, Irshad Ahmad3, Ikramul Haq4, Kashif Prince1, Asad Ullah5, Muhammad Shoaib2, Shahid Zaman6, Amjad Islam Aqib7, Ghazunfar Rashid1, Mahboob Ali1, Imdad Ullah Khan4,Imad Khan5, Naimat Ullah4 and Muhammad Shahid8
...accination with indirect haemagglutination test (IHA). The results showed significantly higher (P< 0.05) immune titer in vitamin E supplemented animals groups against C. perfringens type D epsilon toxin.
...
Beenish Zahid*1,2,7, Javed Iqbal Qazi2, Amir Zohaib2, Asim Aslam3, Raheela Akhter3, Haleema Sadia4, Qurat ul Ain5, Razia Sultana6, Irfan Irshad3 and Sobia Alyas7
...us was confirmed through haemagglutination and haemagglutination inhibition test. Virus isolation rate of NDV from cloacal swab was 12% (6/50) and from oropharygeal swab was 8% (4/50). The 238-bp region of F gene of NDV was amplified by using specific primers of reverse transcriptase-polymerase chain reaction (RT-PCR). Out of 50 field samples 11 cloacal swabs (34%), were positive and out of 50, nine oropharyngeal swabs (18%)...
Muhammad Faisal Ayoob1,2, Zaheer Ahmed Nizamani1, Asghar Ali Kamboh3, Mansoor Ayoob4, Waseem Ali Vistro3 and Abdul Sattar Baloch3*
Saad A. Moussa1, Mohammed Ahmed Mahmoud Abdullah2*, Mahmoud Saied1, Mustafa Saleh1, Mohamed A. Soliman2 and Ali Mahmoud Zanaty2
... allantoic fluids showed haemagglutination activity by using 1% RBCs and also haemagglutination inhibition in case of using NDV reference antiserum, As well as realtime polymerase chain reaction by using standardized NDV specific primers and finally eight viruses were selected for further sequencing for the partial fusion protein, The eight NDVs isolates of velogenic genotype VII and contain the unique cleavage site motif 11...
NawalM. Abdulla1, M. Haroun1*,Mohamed A. Shalaby2and Ahmed A. Elsanousi2
...n eggs
(ECE),haemagglutination (HA), haemagglutination inhibition (HI) and mean death time
(MDT)used in the investigation to reveal virus identity and pathogenicity. Both NDV
isolates were found to be velogenic strains. Real time reverse transcriptase polymerase
chain reaction (rRT-PCR) used as a confirmatory technique, successfully amplified and
detected cDNA fragm...
Afaf. A. Khedr*, Ekram Salama*, A. A. Samy**, Abdella, Y.A.*** and Suzan. K. Tolba***
... done by challenge test, haemagglutination inhibition test, ELISA test for Avibacterium Paragallinarum, and ELISA test, indirect haemagglutination inhibition test and ciliostasis score for infectious Bronchitis virus (IBV). The immune response for the antigenic component of both vaccines show high titre of antibodies, but Montanide ISA-70 adjuvanted vaccine was superior as shown in mean antibody titers against Avibacterium p...
Dalia M. Omar1, Nermeen A. Marden1, Lamiaa M. Gaafar1, Elham A. El-ebiary1, Khalid El-Dougdoug2, Badawi Othman2, Abd Elsattar Arafa3, Hussein A. Hussein4
Sameh Abdel-Moez Ahmed Amer1*, Mohamed Abdel-Aziz Kutkat1, Mohamed Mahmoud Abdel-Baki1, Asmaa Mahmoud Maatouq1, Omnia Mohamed Kutkat2, Hagar Magdy Ahmed1, Khaled Mohamed El-Bayoumi1
...s and further testing by haemagglutination test. Whereas, reverse transcription polymerase chain reaction (RT-PCR) using specific primers to amplify 1681 base pair (bp) of the whole NDV F-gene was carried out to confirm the presence of NDV and then sequenced. Our results demonstrated that, RT-PCR was successfully detecting 62 NDV samples. While, Sequencing and phylogenesis of six selected isolates revealed that, all isolates were virulent NDV with amino acid m...
Aqsa Fayyaz1, Muhammad Kashif Zahoor1*, Asma Ashraf2, Muhammad Asif Zahoor3, Azhar Rasul2, Humara Naz Majeed4, Muhammad Zulhussnain1, Kanwal Ranian1, Bushra Riaz1, Nazia Khalil1 and Attaullah1
... was then titrated using haemagglutination inhibition assay for quantification of NDV. Polyclonal antibodies were produced in experimental rabbits followed by titration. These antibodies were then used to detect the presence of NDV. Sixteen samples were found positive (20%), 62 negative (77.5 %) and two unreactive (2.5 %) for NDV. All of the poultry farms around Faisalabad showed positive NDV (ranging from 1 to 4 samples). We conclud that house flies can play ...
Mohamed Lebdah1, Sahar Abd El Rahman2*, Ahmed Attia3, Reham Karam2, Naglaa Fathy Saeed Awad1, Mohamed Ibrahim El Bagoury1
...s were examined later by haemagglutination (HA) and haemagglutination inhibition (HAI) test. The allantoic fluids of 5 pooled samples expressed positive using specific Newcastle disease virus (NDV) antiserum. The NDV field isolates were confirmed by reverse transcription polymerase chain reaction (RT-PCR) and partial sequencing targeting F gene of amplicon size (362) bp containing the region 112RRQKRF117 motif, which is the ...
Abdullah Iqbal1, Muhammad Abubakar2, Shumaila Manzoor3, Muhammad Kamran Ameen4 and Rani Faryal1*
...route was checked by the haemagglutination test and RT-PCR. All group 2 bucks, developed humoral protection against PPR within the 1st week. Only, 50 percent bucks of group 1 developed humoral protection against PPR after one week of vaccination. The mean percent inhibition value of competition-ELISA of group 2 was half the mean percent inhibition values of group 3 and group 4 after one week of vaccination. There was no antigen shedding through nasal or fecal ...
Soshe Ahmed1*, Mst. Ishrat Zerin Moni1, Maksuda Begum2, Md. Jafar Eqbal3, Md. Shahidul Islam4, Mst. Samira Tanjim1, Mst. Rokeya Sultana1
Olabode, H.O.K1, Mailafia, S1, Martha Echioda-Ogbole1*, Ameh, J.A1, Amadi, K.I1 and Meseko C.A2
Omarl , Dalia M.; El-Ibiaryl , Elham A.; Sadik2, Atef S.; Abdel-Ghaffar2, Mamdouh. H. and Othman2, Badawy A.
...Al) isolates using rapid haemagglutination test (HA). It was found that, all the inoculated isolates caused high mortalities up to 100% for the embryos 24-48hrs post inoculation and gave very strong RBCs agglutination. The haemagglutination positive samples were identified and subtyped antigenically by serological tests using Haemagglutination inhibition test (HI) using standard Al antiser...
*Hanan M.M. Elzahed, Abd EL-Moneim A.A, *Afaf A. Khedr, *Elham A. Elabiare, Waleed S. Abd El-Latif, Reda M.I.
Dilruba Akter Mir1, Md. Sahidul Islam¹*, Mosammat Mahamuda Khatun1, Sharmin Zaman1, Bidyut Matubber², Nazmin Sultana Runa3, Zannatul Ferdous3, Tithe Saha3, Md. Anwar Jahid4
...and recovered birds. The haemagglutination inhibition (HI) test was used to analyze the chicken sera samples for determination of the titer of the antibodies against NDV. The test results showed that the overall seroprevalence was 13.84% (n = 58/419). Layer chickens (17.62%) were more prevalent for ND than broilers (9.38%). However, a chi-square (χ2) statistical analysis revealed that factors such as the age of chickens, seasons, floor condition of the chi...
Dilruba Akter Mir1, Md. Sahidul Islam¹*, Mosammat Mahamuda Khatun1, Sharmin Zaman1, Bidyut Matubber², Nazmin Sultana Runa3, Zannatul Ferdous3, Tithe Saha3, Md. Anwar Jahid4
...and recovered birds. The haemagglutination inhibition (HI) test was used to analyze the chicken sera samples for determination of the titer of the antibodies against NDV. The test results showed that the overall seroprevalence was 13.84% (n = 58/419). Layer chickens (17.62%) were more prevalent for ND than broilers (9.38%). However, a chi-square (χ2) statistical analysis revealed that factors such as the age of chickens, seasons, floor condition of the chi...
Quart ul Ain1, Arfan Ahmad1*, Aftab Ahmad Anjum1 and Mamoona Chaudhry2
...se were also measured by haemagglutination inhibition (HI) test using blood samples collected at an interval of 5 days till the age of 40 days. In this study, none of the vaccines found effective to prevent virus shedding however statistically, the difference in viral load was non-significant (P<0.5) within the local vaccinated groups while significant (P>0.5) when compared local with imported vaccine and control groups. Post challenge infection, virus s...
Yasser Asaad Hameed Al-Shareef, Firas Hussain Kadim Abawi*
...yonated eggs followed by haemagglutination and haemagglutination inhibition assays. Using reverse transcriptase-polymerase chain reaction, the presence of the virus was confirmed and mean death time approach was applied to determine the pathotype of isolates (velogenic). Next, we assessed the most important determinant of ND virus pathogenicity by the analysis of the fusion protein cleavage sites, phylogenetic analysis sand ...
