Ghulam Akbar1*, Muhammad Anjum Zia1, Ali Ahmad2, Neha Arooj1 and Shahneela Nusrat1
...vascular diseases. Among fibrinolytic enzymes streptokinase (SK) is commonly used in the world to cure heart diseases because its low cost and efficacy. It’s a microbial origin enzyme produced especially by Streptococcus bacterial spp. Therefore, it is antigenic in nature due to microbial source which restricts its use. However, it is necessary to truncate antigenic regions present in the SK gene for the removal of streptokinase antigenicity. The basic g...
Ghulam Akbar1*, Muhammad Anjum Zia1, Amer Jamil1 and Faiz Ahmad Joyia2
...ify;">Streptokinase is a fibrinolytic enzyme produced from various bacterial species especially beta hemolytic Streptococci. Among these beta hemolytic strains the Streptococcus equisimilis is being used for industrial streptokinase production. It has 414 amino acids globular chain with 47000 D molecular weight. The fibrinolytic drug is comparatively cheap as compared to all other fibrinolytic...
Saad Tahir, Nadeem Ahmed*, Mohsin Ahmad Khan, Muhammad Akram, Rabia Abbas and Kausar Malik
...K) is a thrombolytic and fibrinolytic protein (47kDa), naturally produced by beta-hemolytic streptococci. Purified SK is used for many blood circulatory complications, i.e., myocardial infarction, ischemic stroke and pulmonary embolism. In this study, human albumin fusion technology has been developed to increase the half-life in-vivo and also invoke less immune response. We designed codon-optimized HSA-SK fusion gene, integrated into -pPICZaB vector, cloned a...
