Muhammad Sarfraz*, Shahida Hasnain
...of plasmid mediated AmpC beta-lactamases (AmpC) has clinical significance due to developing resistance of clinical pathogens against several beta-lactam antibiotics. This study was conducted on 11,725 specimens collected from hospitalized and non-hospitalized patients from July 2013 to June 2016. API 20E system has been used to identify K. pneumoniae and E. coli; preliminary screening for AmpC
Syed Aun Muhammad1, Muhammad Soaib Said2, Shams Ur Rehman2, Muhammad Dawood2, Muneer Ahmed Qazi3 and Nighat Fatima2,*
... of natural compounds to beta-lactamase. Docked energies for diaporthein A and B were -8.02 and -8.41 kcal/mol, respectively. These outcomes revealed that these ligands could be potential drugs to treat infectious diseases.
...
Arifa Mehreen1, Iram Liaqat2,Muhammad Arshad3, Muzzamil Waheed4 and Najma Arshad1,*
...nce was observed against beta-lactam group followed by cephalosporin, lincosamide, tetracycline, macrolides and aminoglycosides. No resistance was observed against vancomycin and linezolid. Among genes involved in host immune evasion, Staphylococcus protein A (spa) was identified most frequently (81%) and proportions of capsular polysaccharides (CPs8), clumping factor A (clf A) andintracellular adhesion A (ica A) were 78%, 68...
Amjad Islam Aqib1,*, Shagufta Nighat2, Rais Ahmed3, Saba Sana3, Muhammad Ameen Jamal4, Muhammad Fakhar-e-Alam Kulyar2 , Naimat Ullah Khan5, Mian Saeed Sarwar5, Muhammad Asif Hussain5, Asadullah5, Attaur Rahman5 and Sadeeq ur Rahman5,*
..., grazing system, use of beta-lactam antibiotics, parity, and poor hygiene were potential risk factors. Altogether, the study concluded high incidence of SCM and isolation rate of staphylococci that were found resistant against most of the commonly used antibiotics.
...
Muhammad Hidayat Rasool1,*, Muhammad Zaheer1, Muhammad Farooque Hassan2, Muhammad Shafique1 and Muhammad Usman Qamar1
...read of acquired Metallo-beta-lactamases (MBLs) among major Gram negative pathogens is an emerging threat and a matter of great concern worldwide. Especially increasing resistance to 3rd and 4th generation cephalosporin and carbapenems is an issue of high importance. Present study was aimed to determine the frequency and antimicrobial susceptibility patterns of MBL producing carbapenem-resistant Gram-negative rods (GNRs) isolated from cli...
Farzana Rashid1, Rabia Masood1 and Mariam Faiz2*
Farheen Aslam*, Hira Lodhi, Rasheeda Bashir, Faiza Saleem and Shagufta Naz
...n hydrolyze all types of beta-lactams. The objective of the study was to screen multiple drug resistant strains of bacteria for New delhi-metallo-beta-lactamase (bla-Ndm1) gene. Blood samples (5ml) of children suffering from different infections, under treatment in a teriary care hospital, were screened for blaNDM-1 gene. Blood samples of 116 patients having tested for multiple drug resistance were analyzed for blaNDM-1 gene...
Kafeel Ahmad*, Muhammad Siqaf, Gul-E-Rana Abid, Ramla Somayya and Umama Qasim
...a lactamases and metallo beta-lactamases in E. coli isolated from fish. Fifty E. coli isolates recovered from fish samples were screened phenotypically for extended spectrum beta lactamases and metallo beta-lactamases production by disc diffusion method using the antibiotics cefotaxime (30 µg), ceftazidime (30 µg), cefepime (30 µg), amoxicillin-clavulanic acid (30 µg), imipenem (10 µg) and merop...
Sara Mohamed Hemeda1, R.H. Sayed2, Hani Hassan1, Sheima A.E.2, Hassan Aboul-Ella3, R. Soliman3*
... conjugate with the free beta-lactam antibiotics supposedly exceeding the permissible limit and existing in the tested samples or vice versa. The control line of the developed lateral flow kits was coated with polyclonal unlabeled goat anti-rabbit antibodies. If the tested milk sample contains a high antibiotic level that exceeds the permissible limits it will be consumed by reaction with the gold anti-β-lactam antibody conjugate in the conjugation pad a...
Mona F. Shousha1*, Aml M. Ragab2 and Salwa M. Helmy1
...nce of extended-spectrum beta-lactamases and plasmid-mediated quinolone resistance genes in Salmonella isolates. First, 800 internal organs (heart, liver, intestine, and yolk sac) were collected from 200 infected broilers to genetically analyze their recovered Salmonella antimicrobial resistant genes. Ten isolates of Salmonella were recovered: two (20%) for each S. enterica serovar Grampian, S. enterica serovar Kentucky, and S. enterica serovar Blegdam and the...
Lubna M. Abdul Kareem*, Ali B. Al-Deewan
...her the isolates contain beta-lactam resistance genes, and detect the bsmB gene for protease and lipase production. We collected 200 milk samples from various areas of Basrah. We found and confirmed Serratia marscecens in 11 (5.5%) of the 200 milk samples by growing them, staining them with gram stain, and then identifying them by PCR with specific primers and sequencing. We also used the disk diffusion method to investigate the antibiotic resistance of the is...
