Aqsa Mehboob1, Noor Khan1,*, Usman Atiq1, Khalid Javed Iqbal2, Rafia Tayyab1, Syeda Suhaira Batool1, Hafiza Saleha Batool1, Sana Amjad1 and Mehwish Tanveer1
...nsity of 25 fish fry per aquaria. There were two treatment groups; T1, T2 and a control (T0) each was replicated twice. Fenugreek as an additive was added in experimental feed at the rate of 0.5% in T1 and 1.0% in T2 while control T0 was without fenugreek supplementation. Fish was fed for six days a week at the rate of 4% of its body weight twice a day. Results showed significant (P≤0.05) hig...
Faiza Ambreen*1 and Muhammad Javed2
...ons, separately in glass aquaria for 70 days at constant laboratory conditions. Peripheral blood erythrocytes were taken on 14, 28, 42, 56 and 70 day of exposure for the DNA damage assessment in-terms of percentage of damaged cells. In general, a dose dependent response was observed in fish erythrocytes with induction of maximum DNA damage at highest concentration (1/3rd of LC50) of pesticide mixture. Statistically significant effects for...
Rimsha Nawaz1, Muhammad Javed1, Sidra Abbas1* and Safina Kousar2
...for 30 days in the glass aquaria with three replications for each treatment. After 30-day exposure of iron chloride, the fish organs viz. kidney and liver were isolated and the peroxidase enzyme activity was analyzed. Activity of peroxidase was assessed by measuring the conversion of guaiacol to tetraguaiacol, spectrophotometrically, at a wavelength of 470 nm. The peroxidase activity was measured in the selected organs exposed to various sub-lethal conc...
Maryyum Khalil1, Hamda Azmat1,*, Noor Khan1, Arshad Javid2, Ali Hussain1, Syed Makhdoom Hussain3, Asim Ullah1 and Sumaira Abbas1
...ted for 90 days in glass aquaria to check the effect of exogenous enzyme (α-amylase) added feed on growth performance, health and hematological analysis of Pangasianodon hypophthalmus (initialaverage weight 32.46±0.36 g). The 40% crude protein diet was prepared by adding feed ingredients along with 0.75, 0.50, 0.25, 0.00 g kg-1 of α-amylase as T1, T2, T3 and T4 (control). At the end of feeding trial, 100% survival rate was ...
Muhammad Javed Iqbal and Muhammad Naeem*
...h culture. A total of 15 aquaria having 20 samples each were arranged in triplicate for 90 days to study the effect of five feeding groups on Length-weight relation and external morphometric variants of Labeo rohita. A total of 75 fish samples @ 5 fish samples per aquaria were analyzed in present study. T1 showed the highest values for mean wet weight 11.32±1.78, Total length 10.54±0.51, Fork length 8.94±...
Studies on amylase and lipase activity in fishes fed with diet containing different feed ingredients
Mirfa Murtaza1, Sajid Abdullah1, Wardah Hassan1*, Khalid Abbas1, Huma Naz1, Muhammad Anjum Zia2
Studies on amylase and lipase activity in fishes fed with diet containing different feed ingredients
...ts. There were two glass aquaria (3×2×2 ft) for each species and ten fish in each
aquarium. Fish in one aquarium were fed on rice polish and fish in second aquarium were fed on corn gluten @ 3 %
of their wet biomass for 70 days. Results showed that fish wet weight varied significantly between the rice polish and
corn gluten fed fishes. The corn gluten fed fish showed significantly better growth performance than fish fed with ri...
aquarium. Fish in one aquarium were fed on rice polish and fish in second aquarium were fed on corn gluten @ 3 %
of their wet biomass for 70 days. Results showed that fish wet weight varied significantly between the rice polish and
corn gluten fed fishes. The corn gluten fed fish showed significantly better growth performance than fish fed with ri...
Maliha Ayub1, Muhammad Javed1, Fariha Latif 1*, Faiza Ambreen2, Komal Sabir1
... of 30 days in the glass aquaria of 70L water capacity. After 30-day exposure, antioxidant (peroxidase) activity was measured in the fish liver, kidney, gills and brain and then compared with control group. The results showed that the antioxidant activity increased significantly in all organs of metal stressed fish as compared to the control fish. Among all test concentrations, the activity of antioxidant enzyme (peroxidase) was significantly higher in the fis...
Fariha Latif1*, Muhammad Javed2, Hammad Ahmad Khan2 and Khalil-Ur-Rehman3
...ights (42 days) in glass aquaria at pH 7.5, temperature 30°C and total hardness 250mgL-1. After each fortnight, fish were sampled from each treatment and different organs such as liver, kidney, gills muscles and brain were taken out for determination of bioaccumulation and oxidative stress in the fish. The results showed that water-borne Pb exposure caused significant accumulation in the fish tissues that increased significantly with increasing ...
Saira Bano, Muhammad Naeem* and Samrah Masud
...ized, kept in triplicate aquaria under controlled conditions in three groups (T1, T2 and T3) and then 5 fish were selected from each treatment at the end of 12 weeks of feeding trial from January to April, 2018, during which fish were fed at the rate of 4% of their body weight. The blood variants were studied like Lymphocytes (LYM), Monocytes (MON), Granulocytes (GRA), Red Blood Cells (R.B.C), Hemoglobin (Hgb), Hematocrit (HCT), Mean Corpuscular volume (MCV), ...
Daniel Masood1, Noor Khan1*, Khalid Javed Iqbal2, Sadaf Dogar1, Abdul Hanan1, Sadia Nazir1, Sheeza Bano1, Azra Anwar2, Sameul A.M. Martin3 and Chris J. Secombes3
...tocked randomly in glass aquaria for a 90 day feeding trial. Fish were fed twice daily with four different iso-nitrogenous diets at a feeding level of 3% of total biomass. The diets contained 26% crude protein in which moringa meal substituted soybean meal at 0% (control), 10%, 20% and 30%, with the groups fed these dietsdesignated as T0, T1, T2 and T3, respectively. The highest weight gain (254.00±4.24 g) was...
Ahmed Saud Alsaqufi1, Sheikh Mustafizur Rahman2,3*, Roshmon Thomas Mathew2, Yousef Ahmed Alkhamis1,2, Md. Moshiur Rahman3,4 and Muhammed Aslam Pathiri2
... were stocked in plastic aquaria (10L) at a rate of 5 individuals/L and reared for one month under four treatments such as 24h light (24L), 24 h dark (24D), 12h light and 12h dark with PVC (12DL_PVC), and 12h light and 12h dark without PVC (12DL) conditions. A total of 108 larvae were sampled for phenotypic traits analysis. The results revealed that complete darkness (24D) significantly improved the overall growth parameters (total length, standard length, cau...
Md. Abdullah Al Mamun1,2*, Shamima Nasren1,3, Sanjay Singh Rathore1 and Kavalagiriyanahalli Srinivasiah Ramesh1
...transferred to the glass aquaria (60 L) and treated with 4 treatments for 15 days: I) Potassium permanganate II) Aquarium salt III) Formalin and IV) Mechanical. The significant lower (P<0.05) parasitic burden were found in mechanical treatment followed by salt, formalin and potassium permanganate. Clinical signs like excessive mucus production, fin erosion, pale gills, and slight hemorrhage were observed in the affected area of skin and operculum. Among the...
Sudip Debnath1, Dipta Sundar Sarker1, Pankaj Kundu1, Md. Shahin Parvez1, Shaikh Tareq Arafat1, Roshmon Thomas Mathew2, Yousef A Alkhamis2,3, Md Moshiur Rahman1, Sheikh Mustafizur Rahman1*
...andomly stocked in glass aquaria feeding ad libitum two times a day. After four weeks of feeding trial, survival of swordtail juveniles was high (≥ 90%) in all treatments and remained unaffected by the dietary treatments (P > 0.05). Differences in growth performance were realized in terms of final weight (g), final length (cm), weight gain (g, %), SGR (%), and metabolic growth rate (g kg-0.8 d-1) (P < 0.05), while swordtail juveniles fed Tubifex and c...
Zahra Hussain, Zulfiqar Ali* and Rida Ahmad
Samia Azad1, Iram Liaqat1*, Riffat Iqbal1 and Uzma Rafi2
...Cd) were tested in glass aquaria having 35 Rohu fingerlings in each. After exposure for six week, sections of intestine, kidney and liver were excised and studied histologically. Results revealed variation in histological changes from mild to severe depending on concentration of exposure. Shrinkage of sub mucosal tissue, enlarged flattened villi, increased apoptosis and degenerated nuclei alongwith missing cytoplasmic boundaries were observed at high concentra...
Shafia Riaz1, Khalid Abbas1*, Tanveer Ahmed1,2*, Huma Naz3, Hina Amjad1 and Shahbaz Ahmad1
...ngs were reared in glass aquaria (twenty fish per population) for 60 days under similar environmental conditions. Condition factor (K) and growth parameters (wet weight, fork and total length) were measured fortnightly. The morphometric data were analyzed using SPSS. Genetics characterization was performed by employing five species specific polymorphic microsatellite markers. Overall, the results of growth trial confirmed that FQB genotype performed maximum wh...
Naeem Tariq Narejo1*, Muhammad Hanif Chandio2, Faheem Saddar3, Majida Parveen Narejo4, Bushra Ainy Dars5, Hafeez ur Rehman Narejo6, Athar Mustafa Laghari2, Shafiq ur Rehman Shaikh3, Shahnaz Rashid7 and Ghulam Abbas7
...aeration provided to the aquaria was continuous for 24 hours, and feeding occurred twice daily. The findings of this study indicate that T-II demonstrated the highest performance in terms of growth metrics, followed by T-III. Conversely, a significant drop in performance was observed in T-I (p < 0.05). The highest temperature, measured at T-III, was recorded as 32 °C during the month of June. Conversely, the lowest temperature, observed at T-I, was reco...
Faris Sahib Imran1*, Layth Hamzah Merzah2, Mohammed Mohsin Kareem3
.... C. demersum is used in aquaria and pond gardens for its fast growth rateability to improve water quality by excess nutrients, and toxins, and can be included in the sheep diet as a protein source at certain levels. The study was conducted in Karbala province, using the Al-Husseiniya River as the source of C. demersum. A total of 40 Awassi sheep were divided into two groups. The experimental group received a diet containing C.demersum as the primary protein s...
ADEEBA SYED1, DILAWAR HUSSAIN2, UZMA RAFI1 & SUMAIRA MAZHAR1*
...mg/L for 96 hrs in glass aquaria. The 96 hrs LC50 value of CPF for Labeo rohita was found to be 0.01 mg/L. During chronic exposure fish were subjected to 1/3rd, 1/5th, 1/7th and 1/9th of LC50 for 30 days. At the end of the trials, tissues from various organs like intestine, liver and kidney were collected and sections were examined under digital microscope. Pronounced histological changes like necrosis, infiltration, atrophy, shrinkage and degeneration of inte...
Nageen Iqbal1,2*, Abdul Mateen2, Laiba Shafique1,*, Huma Naz3, Saif ur Rehman4, Nouman Nazir2 and Qingyou Liu4
...was carried out in glass aquaria for a period of 8 weeks. Ten (n=10) fingerlings of L. rohita were reared per aquaria having capacity of 80-L water. Feed having 100% fish meal was considered as T1 and diets having 30% and 60% replacement of fish meal by bone meal as main protein source was considered as T2 and T3, respectively. Results showed that replacement of 30% and 60% fish meal with bone meal has better effect on growt...
Maqsood Jan1, Muhammad Zubair Anjum1*, Zahir Muhammad1, Misbah Farooq1, Muhammad Qayash Khan2 and Shamim Akhter1
... and stocked in 12 glass aquaria (n=10 fish in each) in a triplicate manner with four different diet groups D1 (control), D2 (2g of L. rhamnosus/kg), D3 (4g of L. rhamnosus/kg), D4 (6g of L. rhamnosus/kg) as feed additive. Statistical analysis was done by using one-way analysis of variance and was applied the Duncan’s multiple range test for the identification of significant differences within the treated groups by using statistical package for the socia...
Sadaf Aman1, Yung Fu Chang2, Javed Iqbal Qazi1*, Rahman Ullah3* and Farah Aman4
...Before stocking, all the aquaria were dried for 3 days. One eighty hundred and eighty fish (Labeo rohita) were stocked in 9 aquaria. Twenty fishes were kept in each group. Before stocking, the fish was treated with KMnO4. In treatment 1 (T1) fish were fed with canola meal along with Lactobacillus rhamnosus (NR-113332.1). In treatment 2 (T2) fish were fed with corn gluten meal along with Lactobacillus rhamnosus. The fish were...
