Search for Adipose

Thermal Physiology and Bioenergetics in Ambient or Seasonal Acclimatization in Apodemus chevrieri

Wan-Long Zhu* and Gao Wenrong

... both in liver and brown adipose tissue, suggesting that A. chevrieri was more sensitive to cold than that of photoperiod. Together, these data suggested that A. chevrieri mainly depend on increasing thermogenic capacity to cope with cold or winter condition.

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Effect of Fasudil Hydrochloride and H2 on the Post-Thaw Viability of Cryopreserved Porcine Adipose-Derived Stem Cells

Maoying Zhu, Yuntao Ji, Xiaoyu Wang, Junying Pu and Changqing Qu*

...of cryopreserved porcine adipose-derived stem cells. Four different combinations of cryoprotectants with and without hydrogen gas (H) (purified H₂ was dissolved into normal cryopreservation solution for 2 hrs under 0.6 MPa were tested including the following groups: control (CK), 100µm GSH, 10µm fasudil hydrochloride (FH) and a combination of the two (GSH + FH). A solution of 10% (v/v) Me₂SO + 20% FBS was used as the standard ...

mRNA Expression of Genes Associated with Puberty Onset in the Adipose and Hypothalamic Tissues of Anhui White Goat

Wenqiao Hui¹, Jishun Tang¹, Dejian Zhu¹, Qian Ban²* and Sheng Chen¹*

...al metabolic cue linking adipose and the onset of puberty. In the present study, we first assessed the morphological changes in adipose tissue at pre-puberty and puberty onset stages of goats, and then determined the role of leptin to activate signaling pathways which modulate the expression of hypothalamic genes involved in reproduction and metabolism. We found that leptin significantly upregulated the LEPR in the

Effect of Butyrate on Genetic Expression of Sirt1/AMPK and Akt/mTOR Axes in Murine Adipose Tissue

Tania A. Shakoori^1,*, Soumble Zulfiqar², Khadija Irfan Khawaja³ and Faisal Masud⁴

..., AMPK, Akt, and mTOR in adipose tissue from mice treated with NaB versus placebo. Four groups of mice (n=27) mice were administered oral NaB, intraperitoneal (i.p) NaB, oral placebo or i.p placebo for 16 days. RNA was isolated from the epididymal fat pads and relative genetic expression of AMPK, Sirt1, mTOR and Akt was measured using real time PCR. Oral NaB group showed decreased genetic expression of AMPK (0.04 fold, p<0.001), mTOR (0.30 fold, p=0.04), Ak...

Frequency of Impaired Glucose Tolerance in Obese Patients

Mian Sajjad Ahmad¹, Somia Iqtadar¹, Sami Ullah Mumtaz^1*, Zafar Niaz¹, Iqra Waheed²and Sajid Abaidullah¹

...s a state of excess body adipose tissue. Impaired glucose tolerance is a risk factor for developing diabetes Mellitus & is associated with hyperglycemia and insulin resistance. To determine the frequency of impaired glucose tolerance in obese patients. This Cross Sectional study was carried out in North Medical Ward, King Edward Medical University/ Mayo Hospital Lahore for 6 months i.e. January to June 2014. Total 160 obese patients visiting outpatient dep...

Nucleotide Polymorphism of Leptin Gene in Anatolian Water Buffaloes

Selçuk Kaplan

...h is highly expressed in adipose tissue. Leptin is one of the most significant candidate gene marker for MAS studies. Therefore, bubaline leptin gene exon 2, part of the intron 1 and intron 2 region were amplified and sequenced to identify nucleotide variations in Anatolian water buffaloes. Sequence analysis were revealed seven polymorphic site (G1072A, T1081C, T1131G, T1143C, T1145G, T1151G and C1221T) and one monomorphic site (C1071T) in Anatolian water buff...

Bioinformatics Analyses of Bovine Adipose Tissue Transcriptome from Lilu Beef Cattle at Different Stages of Growth

Guifen Liu^1,2, Xiaomu Liu^1,2, Khuram Shahzad³, Wei You^1,2, Juan J. Loor^3,* and Fachun Wan^1,2,4,*

...

The accumulation of adipose tissue is largely influenced by the genetic background of cattle. Therefore, we analyzed the transcriptome changes within adipose tissue in beef cattle at different stages of growth. Microarray analysis of data at 12 vs 18 months, 12 vs 24 months, 12 vs 30 months, 18 vs 24 months, 18 vs 30 months and 24 vs 30 months uncovered a total of 10625 differentially expressed genes (P ≤ 0.05; no fo...

The Expression of Cytoplasmic and Membrane Proteins in Dog Adipose-Derived Stem Cells on Different Passages During Cultivation in Vitro

Larysa Kladnytska¹, Anatoliy Mazurkevych¹, Natalia Bezdieniezhnykh², Oleg Melnyc¹, Sergiy Velychko³, Mykola Malyuk¹, Vasyl Danilov¹, Yuriy Kharkevych¹ and Magdalena Gryzinska⁴*

...ymal stem cells from dog adipose tissue during in vitro cultivation. Materials and Methods: Mesenchymal stem cells were obtained from dog adipose tissue by explant method. The cells were cultured in CO₂ incubator by standard procedure. Expression of cytoplasmic and membrane proteins on dog stem cells from fat tissue at the IV^th and X^th passages was examined by immunohistochemical method using...

Effects of Novel Oral and Plasmid-Injected KISS1 DNA Vaccines on Immunocastration and in Inhibiting Mutton Odour of Male Goats

Yan-Guo Han, Jia-Yuan Wu, Ri-Su Na, Wei-Jiang Si, Yu-Qin Han, Yan Zeng, Yong-Ju Zhao and Yong-Fu Huang*

...id in waist subcutaneous adipose tissue were detected using gas chromatography. Immunisation of oral and plasmid-injected KISS1 DNA vaccines induced strong antibody response, significantly suppressed testosterone secretion and reduced scrotal circumference and testis weight. It also significantly reduced the concentrations of 4-methyloctanoic acid and 4-methylnonanoic acid in adipose tissue. These findings indicated t...

Polymorphism and Comparative Expression Analysis of THRSP Gene in Fat-Tailed and Thin-Tailed Sheep Breeds

Q. Sun¹, Q. Liu¹, R. Di¹, Y. Wang¹, S. Gan², S. Liu², X. Wang¹, W. Hu¹, X. Cao¹, Zh. Pan¹, X. Guo¹, Y. Yang³, H.E. Rushdi⁴* and M. Chu¹*

...els in both subcutaneous adipose and tail fat tissues were significantly (p<0.01) higher than the other tissues analyzed. Levels of THRSP expression in liver, subcutaneous adipose and tail fat tissues in Tan sheep (thin-tailed breed) were significantly (p<0.01) higher than that in Altay sheep (fat-tailed breed). The results obtained might expand our understanding of the important regulating role of THRSP ...

Effect of Regular Exercise on Fat Metabolism in Rats Fed on Zinc Picolinate Supplemented Diet

Ramazan Erdogan¹*, Mikail Tel², Vedat Cinar² and Ragip Pala²

...ha;2-glycoprotein (ZAG), adipose triglyceride lipase (ATGL), glucose transporter-4 (GLUT-4) and insulin receptor substrate-1 (IRS-1) in adipose tissue of rats fed on zinc picolinate supplement diet. A total of 42 male 8-week-old Wistar albino rats were randomly divided into 6 groups, each of seven; Control (C), Zinc picolinate (ZP), Chronic exercise (CE), Chronic exercise+Zin picolinate (CE+ZP), Acute exercise (AE), Acute ex...

Analysis of Genomic DNA Methylation in Six Tissues of the Greater Horseshoe Bat (Rhinolophus ferrumequinum)

Sen Liu^1,2, Yue Zhu², Haixia Leng², Ying Wang¹, Xiangju Yin¹ and Yajun Zhao¹*

...chnique, including brown adipose tissue (BAT), brain (B), heart (H), kidney (K), liver (L), and muscle (M) tissues. An average of 67.0% CCGG loci was methylated in each tissue. There were significant methylation differences among tissues in full-methylation (p < 0.001) and total methylation (p = 0.001). Moreover, various unique methylated loci (15–59) were also detected. Non-negligible level of hemi-methylation (31.4%) existed, indicatin...

Immunohistochemical Localization of Leptin and Leptin-Receptor Proteins in Different Tissues of Chinese Alligator, Alligator sinensis

HuaBin Zhang^1,2, YaSen Li¹, Tao Pan¹, Peng Yan¹, En Li¹, Hui Xue¹ and XiaoBing Wu^1,*

...n the adipocyte of white adipose tissue, the gastric gland of the stomach, the lamina propria of the intestine and the interstitial cell and seminiferous tubule of testis, leptin receptor staining was detected in the adipocyte of white adipose tissue, the gastric gland and submucosa of the stomach, the submucosa, muscular layer and intestine villi of the intestine, the granulose cell and follicular membrane cell of the ovary...

Plasticity of White Adipose Tissue in Tupaia belangeri under Food Restriction and Refeeding

Dong-Min Hou¹, Ting Jia², Chun-yan Liu¹, Zheng-Kun Wang¹ and Wan-Long Zhu¹*

...daptive changes of white adipose tissue (WAT) in Tupaia belangeri under food restriction (FR, fed 70% of ad libitum food intake) and refeeding (Re), body mass, food intake, resting metabolic rate (RMR), morphology, the positive expressions of uncoupling protein 1 (UCP1) and Cd137, and the relative expressions of PR domain containing 16 (PRDM16), bone morphogenetic proteins 7 (BMP7), peroxisome proliferator-activated receptor α (PPARα)...

Differentially Expressed Genes Analysis of Brown Adipose Tissue During Cold Acclimation in Male Tree Shrews (Tupaia belangeri) based on RNA-Seq

Dong-Min Hou¹, Ting Jia², Hui-Juan Wang¹, Zheng-Kun Wang¹ and Wan-Long Zhu¹*

...ogenic function of brown adipose tissue (BAT) was known to be markedly elevated when animals were exposed to the cold. In the present study, transcriptome sequencing of BAT in Tupaia belangeri between control and cold acclimation group were carried out by Illumina novaseq 6000 platform. Then the pathways and key genes related to lipid metabolism and energy metabolism were screened out by bioinformatics analysis. The results showed that there were significant d...

Characterization of Stromal Vascular Fraction Harvested from Inguinal Fat of Rabbit for Healing of Full Thickness Burn Wound

Abdul Aziz^1,2, Hamad Bin Rashid¹*, Muhammad Arif Khan¹, Asim Khalid Mahmood¹, Ayesha Hassan¹, Hamid Akbar¹, Sadaf Imran¹, Naveed Hussain¹, Muhammad Umar¹, Muhammad Asif¹, Sajjad Javaid¹ and Mamoona Chaudhry³

...rization of SVF from the adipose tissue of rabbits. Twelve healthy rabbits having body weight of 1000-1500g and of the same age were included in this study. SVF was prepared from bilateral inguinal fat that was harvested under general anesthesia. The harvested fat was enzymatically digested with 0.1% collagenase-I. It was then centrifuged and the SVF pellet was collected. The pellet was characterized through total viable cell count, cell per gram and percentag...

Development of nuclear polyhedrosis virus in silkworms, Bombyx mori Larvae and its impact on sericulture in Punjab, N.W.F.P. and Azad Kashmir

R. H. Jafri Dr., K.M. Saleem, R. Iqbal and Qaisra Fazal

...howed that the nuclei of adipose tissues, tracheal membrane, dermal cells and blood cells developed polyhedral bodies within a week after infection. Nuclear polyhedrosis virus multiplied rapidly and finally occupied the whole cell. Due to rapid increase in number of nuclear polyhedrosis virus, the cells and finally all the tissues disintegrated, resulting in death of the individual. Nuclear polyhecrosis virus infection in silkworm caused heavy losses to poor S...

The Leptin Hormone Administration on the Estrus Intensity and the Appearance Time of Postpartum Estrus in Primiparous Bali Cattle

Desak Nyoman Dewi Indira Laksmi¹*, I. Gusti Ngurah Bagus Trilaksana¹, I. Wayan Sukernayasa¹, Nyoman Oka Widiarta², I. Wayan Nico Fajar Gunawan¹, I Made Merdana³

...nergy reserves stored in adipose tissue increases when there is a negative energy balance after childbirth. Leptin is a hormone secreted by adipose tissue that plays a role in conveying metabolic information to the hypothalamic-pituitary axis of the ovary. Therefore, this study aims to show that leptin can increase the estrus intensity and the appearance time of postpartum estrus in primiparous cattle. This is a true experim...

Investigation of Litter Size in Awassi Sheep and their Correlation to Adipose Tissue Biomarkers

Suroor Hafedth Mohammed, Tahreer Mohammed Al-Thuwaini*

... of litter size with the adipose tissue biomarkers in Awassi ewes. In the current study, 100 sexually mature, not pregnant or lactating, and healthy ewes between the ages of 3 and 4 years were evaluated. The live body weight and age of each animal were determined. Serum was collected to measure leptin and visfatin hormones using ELISA kits made by SunLong Biotech. This study revealed that litter size significantly (P ≤ 0.05) influences the live body weight ...

Litter Size and Its Relationship to Leptin and Visfatin Hormones in Hamdani Sheep

Tamadhur H. Hussein, Layth H. Merzah, Tahreer M. Al-Thuwaini*

...produced and secreted by adipose tissue, including leptin and visfatin. Adipokines increase energy levels and stimulate the production of essential reproductive hormones to enhance reproductive activity in livestock. Therefore, this study examined the relationship between litter size and adipokines in Hamadani ewes. This study examined 140 ewes aged between 3 and 4 years, which were sexually mature, not pregnant or lactating, and healthy. Live body weight, age...

A New Herbal Combination and Trigonella foenum graceum Improve Insulin Resistance, Insulin Signaling Genes, Adipokines Level and Body Weight in Type 2 Diabetic Rat Model

Sana Eijaz, Asmat Salim* and Mohammad Anwar Waqar

...s in skeletal muscle and adipose tissue including insulin receptor (INSR), insulin receptor substrate-1 (IRS-1), protein tyrosine phosphatase-1B (PTP-1B), phosphoinositide 3-kinase (PI3-K), protein kinase B (PKB), and phosphoinositide-dependent protein kinase-1 (PDK-1) and protein kinase C-theta (PKC-θ) genes and on different adipokines level. Wister rats were used to develop type 2 diabetes using high fat diet and streptozotocin and treated with the ext...