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The mRNA Expression of Host Defense Peptides in Chicken Erythrocytes are Highly Related to Tibial Dyschondroplasia and Induced by Recombinant Glutathione-S-Transferase A3 Protein

The mRNA Expression of Host Defense Peptides in Chicken Erythrocytes are Highly Related to Tibial Dyschondroplasia and Induced by Recombinant Glutathione-S-Transferase A3 Protein

Sheng Niu1, Ali-Raza Jahejo1, Fa-jie Jia1, Xin Li1, Guan-bao Ning1, Ding Zhang1, Hai-li Ma1, Wei-fang Hao2, Wen-wei Gao1, Yu-jun Zhao1, Shi-min Gao1, Jian-hui Li1, Gui-lan Li1, Fang Yan1, Rong-kun Gao1, Huan-chun Chen1,3 and Wen-xia Tian1,*

1College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China
2Taiyuan Center for Disease Control and Prevention, Taiyuan 030024, China
3State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China

*      Corresponding author: [email protected]

 

Fig. 1.

Expression pattern of HDPs transcripts in the un-stimulated erythrocytes of 23-d-old broilers relative to the house-keeping gene 18SrRNA.

Fig. 2.

The changes in the expression levels of mRNAs of HDPs in chicken erythrocytes of 12-day-old broilers. Basal diet groups (A, B and C) treated with 0, 20, 50 μg/kg of recombinant glutathione-S-transferase A3 (rGSTA3) protein, and thiram-containing diet groups (D, E and F) treated with 0, 20, 50 μg/kg of rGSTA3 protein. In each panel, different lowercase letters (a–e) indicate statistically significant differences (P < 0.05).

Fig. 3.

The changes in the expression levels of mRNAs of HDPs in chicken erythrocytes of 23-day-old broilers. Basal diet groups (A, B and C) treated with 0, 20, 50 μg/kg of recombinant glutathione-S-transferase A3 (rGSTA3) protein, and thiram-containing diet groups (D, E and F) treated with 0, 20, 50 μg/kg of rGSTA3 protein, respectively. In each panel, different lowercase letters (a–e) indicate statistically significant differences (P < 0.05).

Pakistan Journal of Zoology

October

Pakistan J. Zool., Vol. 56, Iss. 5, pp. 2001-2500

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