Study on the Extracting Technology for Antioxidant Oligopeptides from Donkey Meat by Two-Step Enzymatic Hydrolysis
Lanjie Li1, Jingjing Zhang2, Ruiyan Zhang2, Ning Zhang2, Zixiang Wei2, Guiqin Liu1,*, Riaz Hussain Pasha3, Muhammad Akram Khan4 and Saif-Ur-Rehman5
1College of Agronomy, Liaocheng University, Liaocheng 252000, China
2Institute of Bio-pharmaceutical, Liaocheng University, Liaocheng 252000, China
3Department of Veterinary Biomedical Sciences (Histology), Faculty of Veterinary and Animal Sciences, PMAS-Arid Agriculture University, Rawalpindi, Pakistan
4Department of Veterinary Pathology, Faculty of Veterinary and Animal Sciences, PMAS-Arid Agriculture University, Rawalpindi, Pakistan
5Department of Parasitology and Microbiology, Faculty of Veterinary and Animal Sciences, PMAS-Arid Agriculture University, Rawalpindi, Pakistan
Fig. 1.
Effects of pH (A), enzyme concentration (B), temperature at pH 5.96 (C) and hydrolysis time (D) on DH, PR, and DSA. The pH was 5.96. The enzyme: Substrate ratio was 0.7%. The reaction temperature was 55ºC. Data are presented as mean±SD (n=6). Error bars represent SDs.
Fig. 2.
The DPPH, hydroxyl, superoxide radical scavenging activities of Hydrolysates. Data are presented as mean±SD (n=6). Error bars represent SDs.
Fig. 3.
The molecular weight distribution of Ejiao Oligopeptid and Hydrolysates (3 h).