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Role of Glutathione S Transferase Polymorphism in the Pathogenesis of Cardiovascular Diseases: A Case Control Study

Role of Glutathione S Transferase Polymorphism in the Pathogenesis of Cardiovascular Diseases: A Case Control Study

Muhammad Akram1*, Muhammad Imtiaz Shafiq2, Amber Malik3, Farmanullah Khan4, Munir Ahmad Bhinder5 and Muhammad Sajjad6

1SFINHS, Federal Post Graduate Medical Institute, Shaikh Zayed Medical Complex Lahore
2School of Biochemitry and Biotechnology, University of the Punjab, 54590-Lahore 
3Federal Post Graduate Medical Institute, Shaikh Zayed Medical Complex, Lahore
4Profesional College of Medical Sciences (PCMS), Takhti Nasarti Near PTCL Exchange Chokara (27300) District Karak (KPK) Pakistan. 
5Department of Human Genetics and Molecular Biology, University of Health Sciences, Lahore
6School of Biological Sciences, University of the Punjab, 54590-Lahore, Pakistan.
 
* Corresponding author: aakramskzmdc@yahoo.com

Fig. 1.

(A) DNA extraction of control and disease and (B) agarose gel electrophoresis (2%) of GST polymorphism PCR, showing GSTM1 and GSTT1 alleles of healthy control and CAD patients. Extreme right lane contain 100 bp DNA marker, and extreme two left lane contain amplicons of control individual then followed by two lanes of CAD patients and β globin DNA fragment of 300 bp act as an internal standard.

Fig. 2.

Agarose gel electrophoresis (2%) of PCR-RFLP of the GSTP1 variant Ile105Val (A to G transition). M lane is 100 bp DNA ladder, Lane 1 contain variant GSTP1 (Val/Val), Lane 2 contain variant GSTP1 (Ile/Val) and Lane 3 contain GSTP1 (Ile/Ile). Band of Fragment of 9 base pairs being very small in size not shown in picture.

Fig. 3.

Frequencies of GST genotypes (M1, T1 and P1) among MI patients with reference to healthy individuals.

Pakistan Journal of Zoology

October

Vol. 54, Iss. 5, Pages 2003-2500

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