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Quality Evaluation of Reference Gene Expression on Different Tissues in Adults of Tropical Gar Atractosteus tropicus

Quality Evaluation of Reference Gene Expression on Different Tissues in Adults of Tropical Gar Atractosteus tropicus

Luis Daniel Jiménez Martínez1, Vicente Morales Garcia2Carlos Alfonso Frias Quintana3, Alejandra del Carmen Castillo Collado1, Gloria Gertrudys Asencio Alcudia4, Carina Shianya Alvarez Villagomez4, Emyr Saul Peña Marín4,5, Bartolo Concha Frias1 and 
Carlos Alfonso Alvarez-Gonzalez4

1Laboratorio de Biología Molecular, DAMJ-UJAT, Dirección: Carretera Estatal Libre Villahermosa-Comalcalco Km. 27+000 s/n Ranchería Ribera Alta, C.P. 86205, Jalpa de Méndez, Tabasco, Mexico.
2Laboratorio de Docencia, DAMC-UJAT, Dirección: Rancheria Sur cuarta sección Comalcalco, Tabasco, Mexico.
3Laboratorio de Investigación en Biotecnologia Acuicola (LIBA), Tecnologico Nacional de Mexico Campus Boca del Rio (ITBoca). Boca del Rio, Veracruz, Mexico.
4Laboratorio de Acuicultura Tropical, DACBIOL-UJAT, Carretera Villahermosa-Cárdenas Km 0.5, C.P.86139 Villahermosa, Tabasco, Mexico.
5Cátedra-CONACyT, CDMX, Mexico.
 

*      Corresponding author: alvarez_alfonso@hotmail.com 

Fig. 1.

Agarose gel with PCR products of six candidate reference genes of Atractosteus tropicus. six candidate reference genes of Atractosteus tropicus for qPCR. (A) alfa elongation factor (ef1-α), (B) beta-actin (actb), (C) 18S ribosomal RNA (18s rrna), (D) beta-2-microglobulin (b2m), (E) Tubulin alpha (α- tub) and (F) Glyceraldehyde 3-phosphate dehydrogenase (gapdh).

Fig. 2.

Melting curve analyses of six candidate reference genes of Atractosteus tropicus for qPCR. (A) alfa elongation factor (ef1- α), (B) beta-actin (actb), (C) 18S ribosomal RNA (18s rrna), (D) beta-2-microglobulin (b2m), (E) Tubulin alpha (α- tub) and (F) Glyceraldehyde 3-phosphate dehydrogenase (gapdh).

Fig. 3.

Standard curves for the determination of efficiency (E) and correlation coefficients (R2) of six candidate reference genes of Atractosteus tropicus for qPCR. (A) alfa elongation factor (ef1- α), (B) beta-actin (actb), (C) 18S ribosomal RNA (18s rrna), (D) beta-2-microglobulin (b2m), (E) Tubulin alpha (α- tub) and (F) Glyceraldehyde 3-phosphate dehydrogenase (gapdh).

Fig. 4.

geNorm analysis results of six candidate reference genes of Atractosteus tropicus.

Pakistan Journal of Zoology

February

Vol. 54, Iss. 1, Pages 1-501

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