Quality Evaluation of Reference Gene Expression on Different Tissues in Adults of Tropical Gar Atractosteus tropicus
Quality Evaluation of Reference Gene Expression on Different Tissues in Adults of Tropical Gar Atractosteus tropicus
Luis Daniel Jiménez Martínez1, Vicente Morales Garcia2, Carlos Alfonso Frias Quintana3, Alejandra del Carmen Castillo Collado1, Gloria Gertrudys Asencio Alcudia4, Carina Shianya Alvarez Villagomez4, Emyr Saul Peña Marín4,5, Bartolo Concha Frias1 and
Carlos Alfonso Alvarez-Gonzalez4
Agarose gel with PCR products of six candidate reference genes of Atractosteus tropicus. six candidate reference genes of Atractosteus tropicus for qPCR. (A) alfa elongation factor (ef1-α), (B) beta-actin (actb), (C) 18S ribosomal RNA (18s rrna), (D) beta-2-microglobulin (b2m), (E) Tubulin alpha (α- tub) and (F) Glyceraldehyde 3-phosphate dehydrogenase (gapdh).
Melting curve analyses of six candidate reference genes of Atractosteus tropicus for qPCR. (A) alfa elongation factor (ef1- α), (B) beta-actin (actb), (C) 18S ribosomal RNA (18s rrna), (D) beta-2-microglobulin (b2m), (E) Tubulin alpha (α- tub) and (F) Glyceraldehyde 3-phosphate dehydrogenase (gapdh).
Standard curves for the determination of efficiency (E) and correlation coefficients (R2) of six candidate reference genes of Atractosteus tropicus for qPCR. (A) alfa elongation factor (ef1- α), (B) beta-actin (actb), (C) 18S ribosomal RNA (18s rrna), (D) beta-2-microglobulin (b2m), (E) Tubulin alpha (α- tub) and (F) Glyceraldehyde 3-phosphate dehydrogenase (gapdh).
geNorm analysis results of six candidate reference genes of Atractosteus tropicus.
